LOCUS       c00666_gnlPro..         3087 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PGHLLOJK_666.
ACCESSION   c00666_gnlPro..
VERSION     c00666_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 11:21:06
            Original ID  :: gnl|Prokka|PGHLLOJK_666
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3087
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3087
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:2795]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..2795
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3087
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3087
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..554)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 68.6;
                     E-value: 2.2e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg666_1"
                     /sec_met_domain="Radical_SAM (E-value: 1.9e-34, bitscore:
                     107.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARY"
     CDS             complement(555..2795)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg666_2"
                     /sec_met_domain="Gly_radical (E-value: 1.2e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.3e-282, bitscore:
                     926.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRRVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNSLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             complement(2966..3085)
                     /locus_tag="ctg666_3"
                     /transl_table=11
                     /translation="TGLGLTISRAIVEAAGGEVGFTSKLGVGSTFWAWLPLRP"
ORIGIN
        1 acataccgag ctaacgcctc cagccactcg tcattatccg tatatccggg gacaataaca
       61 tggcgaatcc aagtatctat gccacgtctc tccaattcat ccaagaacag aagcgtatta
      121 tcctgcttga cacccgccag taacggatgt aaatccgggt ttaaggtttt gatgtccaat
      181 aacactaagt ccgcataatc caagacttcc caagcttttg aggtacatac gaaaccagat
      241 gtgtccaaag ctgtgtgaag accttgctct tggcacaaac ggaaaaactc tttcagaaac
      301 tcaggttgca ataaaggttc cccaccggta acagtcaccc ccccacgggc gataaaactc
      361 ttataccgta agacttccgt caatagctcg ccgggagtca tctgatattt caccttccct
      421 ttcgggttcc aagtatccgg gttatggcaa tacaggcaac gcaacgggca gccctgcata
      481 aaaacgacaa agcgaattcc gggaccatct acggtcccga aactctctaa tgaatgtatc
      541 ttacctttta tcacttacag tgaatggtta atggttcttg aaataacatc caactgttgt
      601 tcacgagtca gtttcacgaa gtttaccgca tatccggata cacggatcgt aagttgcgga
      661 tatttctcag gatgttccat agcgtcgatc aacaaatcct tatcaaatac gttaacattc
      721 agatgctgac ctccatccgg agtaaaatag ccatccaata agctaaccag attattgatc
      781 tgcacttcac gctcctttcc caacgtagcc ggagaaacag cgaaagtata agagataccg
      841 tcgtgcgcat gctggaatgg aagcttcgct accgaagcta gcgcagcgat agcacctttc
      901 gtatcacgac cgttcatagg gttcgcgccc ggagcgaaag gagttccacc gatacggcca
      961 tccggtgtcg taccagtctt cttaccatat accacattag aggtgatcgt aaggatggac
     1021 tgcgtaggtt tcgcatggcg ataggtctcg tgctgacgca aatattccat gaacttctcg
     1081 gtgatctcta cagcgatact atccgtacga tcgtcgttat taccgaaagg aacatattcg
     1141 ccctcacgtt cgaaatcaac cgccaagcca cgctcgtcac ggatcacacg tactttcgta
     1201 tcacggatag cagccaacga atcggcaacg atagaaagac cggcgatacc tgtcgcacgg
     1261 atacgctcta catctccatc gtgaagcgac atctcgaatg cctcatatgc gtatttatca
     1321 tgcatataat ggattatctt caaggcgttc acatacactt tcgccaacca gcgcatcatt
     1381 tgctcatact tagccatcac ctcatcgtat tccagatact cgccgcgaac cggctcgaac
     1441 atcggggcta cctgtacgcc gctgcgctca tcccgtcctc cgttgatcgc atataataaa
     1501 catttcgcca aattcgcacg ggctccgaag aactgcatct gcttaccgat cttcatagga
     1561 gaaacgcaac aagcgatacc ataatcatct ccataatccg gacgcatcaa atcgtcgttc
     1621 tcatattgga tagccgatgt atcgatagag accttggcac agaaacgttt ccagttctcg
     1681 ggagcgttct taaaccacag aaccgtcaaa ttgggttctg gtgccggacc tagattatat
     1741 aatgtatgga gataacgata ggaagttctt gttaccaaag aacggccatc cactccttgg
     1801 ccgcccaagg actctgttac ccaaaccgga tcaccggaga acaaatcatt gtattccggc
     1861 gtacgtaaga aacgaacgat acgcaacttg atgatcagct ggtctaccaa ttcttgcgcc
     1921 tcctcttcgg tcaacttacc ctcacggatg tctttctcga tataaatatc caaaaacgta
     1981 gaagtacgtc cgatagacat cgccgcgcca tcttggtctt ttaccgccgc cagataagct
     2041 aagtacacaa actgaactgc ctcacgagcg ttttgtgccg gacgagtcac atcaaagcca
     2101 tagctagcgc acattttctc gaacgctttc aaagccttga tctgttcgga aatctcctca
     2161 cggctctgga tgatctcttc cgtgatctct tggatatcca aactattcaa gaaacgcttt
     2221 ttctcctcga tcaagattgc cgtaccatat aaagcgacac gacgatagtc accaataata
     2281 cgtcctctac catacgcgtc gggcagaccg gtgataatag ccgaacgacg tgcggcaatc
     2341 atctcttccg tatatgcgga gaaaacgcct tcgttatgag ttttacggta cttcgtgaag
     2401 atctctttcg tcatcgggtc caaatgataa ccataagctt ccaagctatt ctcaaccata
     2461 cggataccgc ccttcgggaa gataccacgt ttcagcggag catcggtttg taacccaacg
     2521 atcacctcat tctccttatc aatatagccc gggccatata catcgatccc ttgaggtaat
     2581 tttgtctcgg cgtcataaac tcccttttcc ctctcgacct taaacatctc ggtcagcttg
     2641 ttccagaccc tacgggtctt ctcggaagaa gcgactaaaa aggaatcatc cccttcataa
     2701 ggcgtatagt tactctggat aaaatctctt acattgatct ctcttttcca ggtctcacct
     2761 ttaaagtgat cccagcactc attctcaaat ttcataaaca atcttatcta ataccacatt
     2821 tttaccgccg caaaggtagg agaattatcg tcatatttct gtcacatatg ctacaccagt
     2881 tctaaaatcc ctataaattg cgataaaaaa gcttctcatc cttcactatt ttaaaaggac
     2941 attaccaata aatggggagt cagacctaag ggcgaagagg caaccatgcc caaaaagtag
     3001 atccgacacc aagttttgaa gtaaagccca cttctccccc tgccgcttct acgatagctc
     3061 tggagatcgt caagccaagt ccggtac
//
