LOCUS       c00149_gnlPro..         4479 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GMBFNPJD_149.
ACCESSION   c00149_gnlPro..
VERSION     c00149_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 14:58:51
            Original ID  :: gnl|Prokka|GMBFNPJD_149
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4479
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..159
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg149_1"
                     /sec_met_domain="CT_Vparvula (E-value: 3.2e-17, bitscore:
                     51.0, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="EAFYNPYVAAGMGQITEVINPEETRAKIAFALRTLLNKKEVRPAK
                     KHGNIPL"
     protocluster    1..4479
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:4478]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..4478
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..4479
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4479
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             181..633
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg149_2"
                     /sec_met_domain="OAD_gamma (E-value: 1.4e-10, bitscore:
                     30.5, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLTTLAFAFAQGIANVMNNLNYQVVGIMVVMLCLGGMAVILTVS
                     GSIAAAIQNRAKAKTAAPVAVPAAAPAAAGKPGMTPEMLAILSAAVDSAMTELTPEMVA
                     VISAAVDAGLDGMSHRIVEIKQSPGSGYAAAGRAEIFASHRIRPSR"
     CDS             706..1107
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 58.8; E-value:
                     1.2e-18)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg149_3"
                     /sec_met_domain="Biotin_lipoyl (E-value: 1.2e-19, bitscore:
                     58.8, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKLRITVDGKVFDVSVELLDQVSSTTAAPAPAPVAAPAPAASAP
                     VAAPAPAPAPAPAPAAASAGDVPSPLAGKVVSLDVAAGAPVKAGDQILTLEAMKMNTII
                     YAPASGTLTAFCVNPGDTVQEGQALAKIG"
     CDS             1186..2850
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg149_4"
                     /sec_met_domain="OAD_beta (E-value: 7.4e-160, bitscore:
                     520.2, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLFDSLITFLQGMGVFSLSWQMVGMWGIAILLLYLGVAKQYEPL
                     LMVPIAFGALIANIPDNGMLITQLNQQVISSNEQGEVTATSLNNVGYLRVHVAPLQQAP
                     SKVPANLTTPESRAQYQEIMQQPMQVYPGSQLTVSKIKSVRESQEKAKADAARLGDDSL
                     TVDPNLKDFQNVTEDNGNEPVFLLTNGEGTTVVRQQGVNYFDTSGNRIPVDLKTQKLEP
                     LVVSAAGKYVAVGQHTQELLVTSIHGGLYDWIGLGIKAEIFPPIIFLGVGALTDFGPLL
                     AAPRTLLLGAAAQVGVAATFFMALFMGFTPNESASIGIIGGADGPTSIFLTMKLAPHLL
                     GAVAVAAYTYMSLVPLIQPPIMALLTTKKERLIRMKSLRTVSKSEKLFFAVLVTIVTIL
                     LIPDASPLIGMLMLGNFLRECKVTERLVQASQNEIINIVTIFLGTSVGLTMQGDRFLQS
                     ETLLIILLGIVAFGVATAGGVIAAKIMNLIWRKNPVNPLIGSAGVSAVPMAARVSHNVG
                     QKYDPSNYLLMHAMGPNVAGVIGTAVIAGYYIATLAK"
     CDS             2888..3667
                     /locus_tag="ctg149_5"
                     /transl_table=11
                     /translation="MNSAASFSTPPEEEGPCGDWTYHEWAEAFSTNDLARTLPPGHIAV
                     CRVQTGGRGRFNRKWIGEKGGLWASFTVPLSAPEDTAAGVHWGHLPLVAGLALFNMLRG
                     MGLDSVRLRWPNDLLAGKSKLAGILVERPAENMAVIGIGINVFNDVSSIAGEIKDPPAR
                     LADLVRDCPSLHAVMASLGSHLNQVLSVFSHGGVPSLQEGLSRAWAGERPVSILTDDET
                     VRGIFTGIDDQGNPVLSLPAGTVRTIPAHLINRLLEE"
     CDS             3729..4478
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: AcetylCoA_hydro"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg149_6"
                     /sec_met_domain="AcetylCoA_hydro (E-value: 3.4e-45,
                     bitscore: 143.1, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYKVLTPEEAASLIKHGHHIGLSGFTPAGTAKAVTAALAKIAEA
                     EHEKGNPFQVSVFTGASTGDSCDGILSRAKAIRYRAPYTTNVDFRKAVNNGEIAYNDIH
                     LSQMAQEVRYGFMGNVDIAIIEACEVTADGKIYLTAAGGIAPTICRLADKIIVELNAAH
                     SKSAMGLHDVYEPLDPPYRREISIFKPSDRIGKPYIQVDPKKIVGVVETNWPDEARSFA
                     AADPLTDKIGQNVADFLAADMKRGIIPS"
ORIGIN
        1 gaagcctttt acaacccgta tgtggcggcc ggcatgggac agattacgga agtcatcaat
       61 ccggaagaaa cccgcgccaa aatcgccttc gccctgcgca ccctgctgaa caagaaggaa
      121 gtgcgcccgg ccaagaaaca cggcaacatt ccgctctaac atccatccca acggatttat
      181 atgttactta caacactcgc ttttgccttc gcacagggca tcgccaacgt tatgaacaat
      241 ctgaactatc aggttgtcgg catcatggtg gttatgctgt gcctgggagg catggccgtc
      301 attctgaccg tcagcggctc catcgccgcc gccattcaga acagggccaa agccaaaaca
      361 gccgctcccg tagccgtgcc tgccgccgct cccgctgcgg cgggcaaacc gggcatgact
      421 ccggaaatgc tcgccatcct ttccgccgcc gtggattccg ccatgacgga gcttacgccg
      481 gaaatggtgg ccgtcatctc cgccgccgtg gacgcaggcc tggacggcat gagccaccgc
      541 atcgtggaaa tcaagcaatc cccgggttcc ggctatgcgg ccgcgggcag ggcggaaatt
      601 ttcgcctccc accgcatccg gccttcccgc tgaactactg cattattcac ccttaccccg
      661 cccagtcgga atcatttaac ttaatatccc ataccacaca ctattatgaa gaaacttcgc
      721 atcaccgtag acggcaaagt atttgacgta tccgtagaac ttctggacca ggtgtcctcc
      781 accactgcgg cgccggcccc cgcacctgtg gccgctcccg ctcctgcggc ttccgcaccc
      841 gtggccgctc cggccccggc tcccgcacct gctccggctc cggccgccgc cagcgcagga
      901 gacgttccca gcccgcttgc cggtaaggtc gtctccctgg acgtggcagc aggcgcaccc
      961 gtgaaggccg gtgaccagat tctgacgctg gaagccatga agatgaacac catcatctac
     1021 gctcccgcct ccggcacgct caccgctttc tgcgtgaacc cgggcgacac ggttcaggaa
     1081 ggacaggcac tggccaaaat tggttaatcc cggcggcggc agcccggaaa cgccaacagt
     1141 accgggctcc gactcaaata ataaaccgct acaaaaatat tccctatgag tctttttgat
     1201 tcattaatca cctttttaca ggggatgggc gtcttctccc tatcatggca gatggtaggg
     1261 atgtggggca ttgccattct cctcctgtat ctgggtgtag ccaagcaata cgaaccgctc
     1321 ctgatggttc ccattgcttt cggcgccctc attgccaaca ttccggataa cggaatgctc
     1381 atcacccagc tgaaccagca ggtcatctcc tccaatgaac agggggaagt aaccgccacg
     1441 tctctgaaca acgtggggta cctgcgcgtt cacgtggctc cgcttcagca ggctccctcc
     1501 aaggtgccgg ccaatctgac cacgcctgaa tcccgtgccc agtatcagga aatcatgcag
     1561 cagcccatgc aagtttaccc cggcagccag ctgaccgttt ccaagatcaa gtccgtgcgg
     1621 gaatcccagg aaaaagcaaa agctgacgcg gcccgcctgg gcgacgacag cctgacggtg
     1681 gaccccaacc tgaaggattt ccagaacgtg acggaagaca acggcaatga accggtcttc
     1741 ctgctcacga acggagaagg gaccaccgtc gtccgccagc agggcgtcaa ttactttgac
     1801 accagcggca accggattcc ggtggatctg aaaacccaga agctggagcc cctggtggtt
     1861 tcagccgcag gcaaatacgt ggccgtaggc cagcataccc aggaactgct ggtcacctcc
     1921 atccacgggg gcctgtatga ctggatcggg ctgggcatca aggctgaaat tttcccgccc
     1981 atcatctttc tgggcgtagg agccctgacg gacttcggcc cgctgcttgc cgctccgcgc
     2041 accctgcttc tgggcgcagc ggcccaggtg ggcgtagccg ccacgttctt catggccctg
     2101 ttcatggggt tcaccccgaa tgaatccgcg tccatcggca tcattggcgg cgctgacggc
     2161 cccacctcca tcttcctgac gatgaagctg gccccgcacc tgctgggcgc cgtggccgtg
     2221 gccgcataca cgtacatgtc cctggttccg ctgatacagc cgcccatcat ggcgttgctg
     2281 acgaccaaga aggaacgcct catccgcatg aaatccctgc gaacggtcag caagagcgaa
     2341 aaactgtttt tcgccgtgct ggtaaccatt gtcaccattt tgctgattcc ggacgcatcc
     2401 ccgctgatcg gcatgctgat gcttggcaac ttcctgcgcg aatgcaaggt gacggaacgc
     2461 ctggttcagg cctcccagaa tgaaatcatc aacatcgtca ccatcttcct gggaacctcc
     2521 gtggggctca cgatgcaggg cgaccgcttc ctgcagtcgg aaacgctgct gatcatcctt
     2581 ctgggtatcg tcgccttcgg cgtggcaaca gcgggcggag tgattgccgc caagattatg
     2641 aacctcatct ggcgcaagaa tccggtcaac cccctgatcg ggtccgcagg cgtctccgcc
     2701 gtcccgatgg cagcccgcgt ctcccacaat gtgggccaga agtacgaccc ctccaactac
     2761 ctgctcatgc acgccatggg cccgaacgtt gcaggcgtga tcggcaccgc agtcatcgcg
     2821 ggctactaca tcgccactct ggcgaaataa ccagaattga aaattactcc cggcctcggc
     2881 ccgtcccatg aattccgcag cctcattttc cacccctccg gaggaggaag gaccatgcgg
     2941 cgactggact tatcatgaat gggccgaggc tttttccacc aatgatctgg cccgcaccct
     3001 ccccccgggg catattgccg tctgccgtgt tcagacagga ggccgcggca ggttcaaccg
     3061 caaatggata ggggagaaag gcggcctgtg ggcatccttc accgtccccc tgtccgctcc
     3121 tgaggataca gcggccggcg tacactgggg ccatttgccc ctggttgcgg ggctggccct
     3181 gttcaacatg ctccgcggca tggggctgga ttccgtgcgc ctgcgctggc cgaacgacct
     3241 gctggcgggc aaatccaaac tggcgggaat actggtggaa cgcccggcgg agaacatggc
     3301 cgtcatcggc atcggcatca atgttttcaa tgatgtatcc tccatcgccg gggaaatcaa
     3361 ggaccctccg gcaaggctgg cggatcttgt tcgggactgc ccttccctgc acgccgtcat
     3421 ggcttccctg ggcagtcatt tgaaccaggt tctttccgtc ttttcccacg gcggcgtccc
     3481 ctcccttcag gaggggctgt cccgggcatg ggccggagaa aggccggttt ccattcttac
     3541 ggatgacgaa accgtccggg gcatttttac agggattgac gatcagggca atcctgtgct
     3601 atcccttccg gccggcaccg tccgcaccat tccggcgcac ctgatcaaca ggctcctcga
     3661 agaatgagcc taccgctccg tttttacacg gggtttcttt cttttttcat ccaacaaaaa
     3721 tcactgacat gagttacaag gtactgacac cagaagaagc agcaagcctg atcaagcatg
     3781 gccaccatat tgggctgagc ggattcacgc ccgccggaac ggccaaggcc gttacggctg
     3841 cgctggcaaa aatcgcagaa gcggaacatg agaaggggaa ccctttccag gtgagcgtct
     3901 tcaccggtgc ttccaccggc gattcctgcg acgggatcct ttcccgggcc aaggcaatcc
     3961 gctaccgcgc tccctacacc accaacgttg atttccgcaa ggcggtgaac aacggggaaa
     4021 tcgcctataa tgacatccat ttgtcccaga tggcccagga agtgcgctac gggttcatgg
     4081 gcaacgtgga catcgccatt attgaagcct gtgaagtcac ggccgacggc aaaatttacc
     4141 tgacggccgc gggcgggatc gctcccacca tctgccgcct ggcagacaaa attatcgtgg
     4201 aattaaacgc cgcccacagc aagagcgcca tgggcctgca cgacgtttac gaacccctgg
     4261 atcctcccta ccgccgcgaa atttccatct tcaaacccag cgaccgcatc ggcaagccgt
     4321 acatccaggt tgacccgaaa aagattgtgg gcgttgtgga aaccaactgg ccggatgaag
     4381 cccgttcctt cgccgcagcg gatcccctga ccgataaaat cgggcagaac gtggccgatt
     4441 tcctggctgc cgacatgaaa cggggcatca ttccctcca
//
