LOCUS       gnlProkkaFKIBLBHE_55   13542 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FKIBLBHE_55.
ACCESSION   gnlProkkaFKIBLBHE_55
VERSION     gnlProkkaFKIBLBHE_55
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-18 04:36:35
            Original ID  :: gnl|Prokka|FKIBLBHE_55
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 8934
            Orig. end    :: 22476
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..13542
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[5000:8542]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      5001..8542
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..13542
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..13542
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             51..740
                     /locus_tag="ctg55_16"
                     /transl_table=11
                     /translation="MKLGIMQPYFMPYIGYFQLIKAVDKYVVYDDVNYIKGGWVNRNNI
                     LVNGEKKMFTITLKGASAYKHFNEIEIGDDFRKFMKTLELNYSKAPFYHETMELLYRII
                     MFPNRQLALFLANSFKEILDYLNIQTEILLSSDLPKDCSLKGKDKVLHICKLLSADTYY
                     NAIGGKQIYNKEDFMQNGINLKFVDTIIKRYPQQSKEFVPYLSIIDVLMNNSKEETCML
                     LDSFSLQ"
     CDS             740..1702
                     /locus_tag="ctg55_17"
                     /transl_table=11
                     /translation="MSIGGYFELEIREGEHFHQGAIKLSTARQCFEYILKARRYTKVYI
                     PYYTCDVIIEPLMKLNVKYEYYHINSLLDPVFDKKIESSEAFLYTNYFGLKQDTVLYLS
                     RFYPNLIVDNAQAFFAKPIEGVDTFYSARKFFGVPDGAYLYTTALLDIDFPIYNPIDNI
                     NYLIGRITDSAEGYYKDFQESEERMSNQEIHRMSVLSDKILSSLDYVSIIERRRFNYHL
                     LYSALTAFNKFDFSISENQNVPLIYPFYTSKAGLRQQLILEHVYVPQYWQNVVNKIDAK
                     CLEYSIANNMIALPIDQRYDEKTMNILIEKISNLNNHLS"
     CDS             1918..2694
                     /NRPS_PKS="Domain: NAD_binding_4 (23-160). E-value:
                     3.4e-07. Score: 21.4. Matches aSDomain:
                     nrpspksdomains_ctg55_18_NAD_binding_4.1"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1010:NAD-dependent epimerase/dehydratase (Score: 88;
                     E-value: 1.3e-26)"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg55_18"
                     /transl_table=11
                     /translation="MLLSAQIPSYMDTYCPIKYLDSIVIGGFNVLEYCRKCHVDRIIYT
                     QTVFDISLYSHDTILKPDMKPNFSYTGDHAIYVISKNTALDLIEHYYQEYGLKKFIFRL
                     PTIYSYSPYPYYFPNGVKTKRPIYQMIENAQKGLPIEIWGDPHYAKDMVHVNDFSQMLC
                     KAVEVEQESGFYNVGTGMPITLEEQIRTIISVFSPKESPSEVIYRPDKVCGGGYIMNIE
                     NAKKELGYVPQYDCRKLFEDYKSEMEIKRFAELRLK"
     aSDomain        1987..2397
                     /aSDomain="NAD_binding_4"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg55_18_NAD_binding_4.1"
                     /evalue="3.40E-07"
                     /label="ctg55_18_NAD_binding_4.1"
                     /locus_tag="ctg55_18"
                     /protein_end="160"
                     /protein_start="23"
                     /score="21.4"
                     /tool="antismash"
                     /translation="VIGGFNVLEYCRKCHVDRIIYTQTVFDISLYSHDTILKPDMKPNF
                     SYTGDHAIYVISKNTALDLIEHYYQEYGLKKFIFRLPTIYSYSPYPYYFPNGVKTKRPI
                     YQMIENAQKGLPIEIWGDPHYAKDMVHVNDFSQ"
     CDS             2736..3635
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg55_19"
                     /sec_met_domain="Glycos_transf_2 (E-value: 3.3e-24,
                     bitscore: 76.9, seeds: 256, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTYNHEKYIRQCLDGLIMQKTEFDFEILVHDDASSDATQSIINEY
                     VVAYPNLIKPILQIRNQYSQGISPLRQILFPLVKGKYVALCEGDDYWTDPLKLQKQVNA
                     MESDHSLAGCAHQSLVFYENGTPSHMFHHVKKQILKTKDLLDVRLFHTASFLFKKEVLD
                     YIKDFNSSLYAGDRFLFLACSLKGNILYMPDVMCCYRKNAMGVTSRVSYSLHVLDLKVP
                     EYFHNKDLKFPYYRYLAFVHRAIFSESLDISLKKYIYHFTLFVKYSFSYFPKNLKDIYK
                     ALYVSFWWLTKGQKYRIK"
     CDS             3881..5011
                     /locus_tag="ctg55_20"
                     /transl_table=11
                     /translation="MKVTTDNSFYNLVIGNNKMILLLMLACIVTYYYAMKFALVIELYG
                     AGEGRMMRFEAGELFTSNLEVYLYDFLIKPFTQFVMLIVACMLLSKIKRRILLLSVLFL
                     FLYASVGNGRGGLIVFLFYCFIIYYLRRELYYINLLASKRKKWNFGVVSFVCLFVGVGI
                     IVVVISYMTAQRMGFDTFSAEVVKIGFEELKSQAVWYFLGPFRALDYSFRANYESITGF
                     WYGRATMGGIDNLLKLVIHYLGGSYTIAHDEIGHLLQNSKISVGNGIIFNYAYTNIIFH
                     YLDFGYVGVLLFPFLYGFIVRYSIYKFTQKPTLLTLGIMIYLVYISMFNIFSWEFQKPS
                     SIIYVLLLLFFQYVYSHKFVILKLNGKKKGLCRHEC"
     CDS             5001..6278
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 202.7;
                     E-value: 1.2e-61)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg55_21"
                     /sec_met_domain="CTP_transf_like (E-value: 1.5e-12,
                     bitscore: 39.1, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 2.2e-119, bitscore:
                     388.9, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 7.3e-64, bitscore:
                     206.7, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSADIIHPGHLNIIHEASKLGHVTVGVLTDSAIASYKRLPYLNFD
                     QRSLIVLNLKGVDEVIPQTTLDYVPNLELIRPDFVVHGDDWKEGIQQETRQRVIDTISK
                     WGGKVIDVPYTKGISSTLLNTRLKEIGTTPEIRLKRLKRLIAAKSIVRICESHSGLTGL
                     IIENTSVEVNGVKREFDGMWSSSLTDSTSKGKPDIEAVDLTTRLHDLNDALECTTKPVI
                     FDGDTGGKIEHFVFTVRTLERLGISAVIIEDKIGLKKNSLFGTDAVQTQDTIEGFCDKI
                     QAGKRAQITDDFMIIARIESFIAGKGLDDALGRALAYIKAGADGIMIHSKDKSGEDIKN
                     FCLALRKIHKSIPIVVVPTTYNHISEDELASWGVNVVIYANHMLRSAYPAMLNTAKSIL
                     ANARSYEANDLCMPIKEILELIPGTK"
     CDS             6310..7440
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 69;
                     E-value: 6e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg55_22"
                     /sec_met_domain="TPP_enzyme_N (E-value: 5.1e-17, bitscore:
                     53.2, seeds: 75, tool: rule-based-clusters)"
                     /sec_met_domain="TPP_enzyme_C (E-value: 8.5e-21, bitscore:
                     65.5, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISPNIFVDSLGAMQMDFFTGVPDSLLKNICAYLTDHLASEHNII
                     AANEGAAIGLATGYYLATRKIPVVYMQNSGLGNAVNPLMSLTDKEVYNIPLLLLIGWRG
                     EPGVKDEPQHIKQGKVTLPLLEAMGIRYAVLSQDEKKFISQLNVAKSYMDETREPFAFV
                     IQKDTFLEYSSENCEDERLSLKREAAIHFIAASLTEKDIIISTTGMISRELFEYRESTS
                     LEHERDFLTVGSMGHASQIALGIALKKRDCKVYCFDGDGSTLMHMGSLAIVGSLHPENY
                     VHVVFNNGAHDSVGGQPTVALDIDLCKIAQACGYEIVLSVSDNEGLCEALKKIQQIRGP
                     ILLEVKVRKGARKNLGRPANTPIQNKEAFMLFLDNL"
     CDS             7448..8542
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Fe-ADH"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg55_23"
                     /sec_met_domain="Fe-ADH (E-value: 4.3e-76, bitscore: 247.2,
                     seeds: 218, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MEQRFFLGESSIKELIPILQEYTPDHIFLVRGKKSYEKCGAQAVL
                     ESVFVKLACGVTEFFDFEANPKIEDMEKGLSLLKEYPTNMIIAIGGGSVLDMAKLLRFF
                     YSYSGNPTGGEFEKKRQLIPLITLPTTAGTGSEATHFSVLYKDKVKYSVEHDAILPDVA
                     IVYPKFTYGNSKYLTSCTGFDALAQAIEAYWNVNATEESDKYAAKAIKLLWPNLPLVVN
                     SPTAEARNKVSEGSYWAGRAINITKTTAPHAFSYSFTTDYGYPHGHAVALTFPFFMRYN
                     IGNSDILLNPNINPYLYKIKMQKMLQWLNMECVDVAIAMKEYIRNLGLDIDISQIDISK
                     IVSSVNIQRLINNPYKVEAICLEE"
     CDS             8527..9243
                     /locus_tag="ctg55_24"
                     /transl_table=11
                     /translation="MSRGISEKEQRWLLVLSQVIEVLDKNNLLYFIDTGTLLGAVRDKM
                     FIPWDNDIDLAVIGGDTSTTNLLKVSKLLYALGFYVTLTKKEIAICSRDGVNLGIKLYK
                     KQASHYEACMGKVYRHRLFYSINLYLSGEVLYKKGFSFKYKAISLFVKFSQVFKLKLSC
                     IWQMKLLFWAKYDEKTVIIPFEFVDAIIDYNFYNIIVKVPNKPERYLAYRYGENWKVPK
                     QDYDYMIDDKSIINID"
     CDS             9740..10513
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glycos_transf_1"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg55_25"
                     /sec_met_domain="Glycos_transf_1 (E-value: 6.9e-13,
                     bitscore: 39.8, seeds: 63, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNDSPYFQRMSWKELMIEKARNLYLYYYFKRGVTAFWTENPYISE
                     KVKHLFPTIKAYTVSNYYNQVFDDENTQIEHALPSFDGCTLLSINAPYPHKNLAIAIEV
                     ARYLKKHYSEFSFRFVFTVTENDLPPIPSYLREHFCLIDKVSVAECPSLYRQADIMFQP
                     TLLECFTATYVEAMRMQVPILTTDLPFAHGLCGNAAVYYSPLDAEDCAKSIYKMAVDTS
                     FQDYLVGEGIKQLKSFDNYNRRAEKLIDLISNLDD"
     CDS             complement(10531..11679)
                     /locus_tag="ctg55_26"
                     /transl_table=11
                     /translation="MAKIVFFSKGAIHSLFLSCCIIQIKDLFTLDFRTLRLFLHPEVFA
                     DEAEFQKKLFHALHIQAGGVMAVIVLYTGNILIAFREELVIVQVTGVARDAVVVAHVDS
                     LCHLFAGYQCLIQFLAVARADGLHLRFTVFRINLRVCFLQGLRQYIQGGGRSLLHEQVT
                     VMAVLKRIDYQIHGIVQRHHEAGHVGVRYRNGPAVLYLLHPQGNYRTAACHHIAVTGTA
                     DGRTGFLTQRTALGYGNLLHHGLADTHGIDGIGGLVGRKHHHILYTMRYGRFQYIVRTD
                     HIGLHSLQREELATGNLLQGGSGKYIIHSHHGTVYRLAVTDITNVKFYFGILQVMTHVI
                     LLFLVTTKNTNLLDIRIKETTQYSVTKTAGTSGYKEDFVFKY"
     CDS             11681..11923
                     /locus_tag="ctg55_27"
                     /transl_table=11
                     /translation="MKLIIENMAATVLNSVQQHLLKMFAFDGSEERLNEVKEVLTKYFS
                     QKLDDRLNDLWDSGVLNQEKLDELRKIDVRTLPKR"
     CDS             11920..12324
                     /locus_tag="ctg55_28"
                     /transl_table=11
                     /translation="MNRIVLDTNSLIQCISPKSRYRKIWDCFLDGTNQLCVSDEILNEY
                     EEILERLAGVEIAKLVIDVILNSPYTLFFTPYYNFNLIKSDPDDNKFVDCAVVANAKFI
                     VTEDHHFHILKQYNFPKVSFIGLDDFCHSL"
     CDS             complement(12374..13051)
                     /locus_tag="ctg55_29"
                     /transl_table=11
                     /translation="MSERPYQFHNAAQGNGMYYGHFAIITVAVFPAAHGRDKLVHHIVY
                     VNQVHHHRRVVHPDGLVMGDVMAESGHRAVIVGAAPFAENIREAVHQYLRPRFLGVFEQ
                     QLLPCPLRFTVRVVECGLRGRGDHYRTGIAVFPQCVEQRGGEAEVPFHKLRRILGTVHA
                     RQIEDKVSLPAIDIQFGRVTVHVILVNGKFLTCNAVIAGFPVTDVVQLGYKVPADEPSG
                     AGH"
     CDS             complement(13121..13411)
                     /locus_tag="ctg55_30"
                     /transl_table=11
                     /translation="MRIAGNHLHDLHPPAVYLEADNLLRTYLAELYQTVPAHHHKLLVL
                     GMVPVLAFRNARLGDVHRELPAVGSTDNLRKTAAVVHIHFQGIAEPFFRQV"
ORIGIN
        1 tgtggattgt cgaatgaaga tgttgaaaga attttaaatc tgatattgaa atgaaattag
       61 gtataatgca accttatttt atgccttata taggttattt ccagctaatt aaggctgttg
      121 ataaatacgt tgtatacgat gatgttaatt atattaaagg agggtgggtg aatcgtaaca
      181 atatattggt aaatggtgaa aagaaaatgt ttactatcac tttgaagggg gctagtgcct
      241 ataaacattt caatgaaata gagataggag atgattttcg taaatttatg aaaacattgg
      301 aattgaatta ctctaaagct cctttctatc atgaaacaat ggaattgttg tataggataa
      361 ttatgtttcc aaatagacaa ttggctctct tcttggctaa tagttttaaa gaaattttag
      421 attatctaaa tattcagact gaaatattgc tatcttcaga tttacctaaa gattgctctt
      481 taaaaggaaa ggataaagtt cttcatattt gtaagttact atcggcagat acttattata
      541 atgctatagg tggaaaacaa atctataata aggaagactt tatgcaaaat ggtattaatt
      601 tgaaatttgt agatacgata ataaaaaggt atccccagca atctaaagaa tttgttccat
      661 atttatcaat aatagatgta ctaatgaata attctaaaga agaaacttgt atgttattag
      721 attcatttag tttacaatga tgtcaatagg tggctatttt gaactggaaa tacgtgaagg
      781 agaacatttt catcaaggtg caataaaact gagtactgct cgtcaatgct ttgagtatat
      841 attaaaagct agacggtata caaaagtgta tattccatat tacacttgtg atgtgataat
      901 agaacctctc atgaaattaa atgtaaagta tgaatattat catataaata gtttactgga
      961 tcctgtgttt gataaaaaaa tagaaagctc agaggctttt ctatatacta attatttcgg
     1021 cttaaaacaa gatactgttc tttatttatc tcggttttat cctaacttaa tagttgataa
     1081 tgcacaagct ttttttgcaa aaccaattga aggggtagat acattttatt ctgcccgtaa
     1141 attttttgga gttccagatg gcgcttattt gtacacaact gcgttactgg atattgattt
     1201 cccgatttat aatcctattg ataatattaa ctacttgata gggcgtataa ctgactctgc
     1261 agaggggtat tataaagatt ttcaagaatc tgaagaaagg atgtcaaatc aagagataca
     1321 tagaatgtct gtattgtctg ataaaatttt gtcttctttg gattacgtct caataattga
     1381 aaggcgtcgt tttaattatc atcttctcta cagtgcatta actgcattta ataaatttga
     1441 ttttagtatt tctgaaaatc aaaatgtccc gttgatatat cctttttata cgagcaaagc
     1501 tgggcttcgc caacaattaa tattagagca cgtatatgta cctcaatatt ggcaaaacgt
     1561 agtgaataag atagacgcta aatgtctgga atactcaatc gcgaataata tgattgcatt
     1621 gcctatagat cagagatatg atgaaaaaac aatgaatata ttaattgaaa aaatatctaa
     1681 cttaaataac catttatcat gaaaaagaaa attattattt ttggagcaac cggtaataca
     1741 gggtcttatt tgttagaata cgcattaaat ttctttaata aggaagagta tgaaataata
     1801 gcttcaggac gtcgcgaaac tacttttttt tccaaaaggg gaataccata ttattctgtg
     1861 gacattacga aggaagaaga ttttgataag cttccaatgg aaaatataca tgcagttatg
     1921 ctactctctg ctcaaatacc ttcttatatg gatacttatt gccccataaa gtatttagat
     1981 tctattgtaa taggaggatt taatgtatta gagtattgcc gcaaatgtca tgtagatagg
     2041 ataatttata ctcaaaccgt ttttgatatt tcattgtatt ctcatgatac tattttaaaa
     2101 cccgatatga aaccaaattt tagctatacg ggtgatcatg ctatatatgt aatttccaaa
     2161 aatactgctt tagacttgat agagcattat tatcaagaat acggtttgaa aaaattcatt
     2221 tttaggcttc caacaatata tagctatagc ccttatccgt actactttcc taatggagtc
     2281 aagacgaagc gacctattta ccaaatgatt gaaaatgctc agaaaggact acctatagag
     2341 atatggggag atcctcatta tgcaaaggat atggttcatg ttaacgattt tagtcaaatg
     2401 ttgtgtaaag ctgttgaggt ggaacaagaa agtggctttt ataatgtggg tacaggaatg
     2461 ccaattacat tagaagaaca aattcgcact attattagtg ttttttcgcc taaggagtca
     2521 ccatctgaag ttatatatag accagacaaa gtttgtggtg gtgggtacat aatgaacatt
     2581 gaaaatgcaa agaaagaatt aggatatgtg cctcaatatg attgtcgtaa gttgtttgag
     2641 gattataaaa gtgaaatgga aataaagcgt tttgctgaat taagattaaa ataannnnnn
     2701 nnnngaacgg ttcaataaca gttagcatat gttgcttgac gtataatcat gaaaaatata
     2761 taaggcagtg tttagatggc cttataatgc aaaaaacaga atttgatttt gaaattcttg
     2821 ttcatgatga tgcgtcttct gatgctactc agagtattat taatgaatat gttgtggcat
     2881 atcctaatct tattaaacca attcttcaga ttcgaaacca gtattctcaa ggtatttctc
     2941 cgttacgtca aatattattt cctttagtaa aaggtaaata tgttgcgctt tgtgaggggg
     3001 atgattattg gactgatccg ttgaagttac aaaaacaagt aaatgcaatg gagagtgatc
     3061 attcgttagc aggttgtgcg catcaatctt tagtttttta tgagaatgga actccatccc
     3121 atatgtttca tcatgtgaaa aaacagattt taaaaactaa agacttattg gatgtaagac
     3181 tttttcatac agcgtctttc ctttttaaaa aagaagtact tgattatatt aaagatttca
     3241 atagtagttt atatgctgga gatcgttttc tgtttttggc ttgttcgttg aaaggaaata
     3301 ttttatatat gccagatgta atgtgttgtt ataggaagaa tgcaatggga gttacttcta
     3361 gagtttctta ttcattacat gtattagacc taaaagttcc tgaatatttt cataataaag
     3421 atttgaaatt tccttattac agatatttgg catttgttca tagagctata ttttcagaat
     3481 cacttgacat ttccttaaag aaatatatat atcattttac tttatttgtg aaatattctt
     3541 tttcttattt tcctaagaat ctgaaagata tctataaggc tttatatgtt tctttctggt
     3601 ggttgacaaa gggacaaaag tatagaatca aatgattgct ataatttttc ttattctatt
     3661 cttaattcag attttgatat ctaataggct tataattgat tctttttcaa gaaaaataag
     3721 tttcatatat ttattgtggt ggggaactgc tattgttatt tctacttttg atccagctga
     3781 actatattct gtgtcaatga aaacatatat gttattattg atcaatgttt gggcgtttaa
     3841 ttggggattt ctttgttttg gaaaaataaa aaaaatacga ttgaaggtta caacagataa
     3901 tagtttttat aatctggtta ttggcaataa taagatgata ttacttctta tgttggcatg
     3961 tattgttact tattattacg caatgaagtt tgctctagtt attgaactat atggagcggg
     4021 cgaaggtcgg atgatgagat ttgaggctgg tgaacttttt acgagtaatt tagaagtata
     4081 tctatatgat tttctaataa aaccttttac acagtttgtt atgctaattg tagcgtgtat
     4141 gctactttct aagattaaac gtagaattct gttgctatcg gtgctttttc tgtttttata
     4201 cgcatctgtt ggtaatgggc gtgggggatt gattgttttt ctattttatt gttttatcat
     4261 ttattattta agaagagaat tgtactatat caatttgtta gccagcaaaa ggaaaaaatg
     4321 gaattttggt gtagtttctt ttgtatgctt gttcgttggc gttggaataa tagttgtggt
     4381 tatttcatat atgacagctc aaagaatggg atttgataca tttagtgctg aggtcgtaaa
     4441 aataggtttt gaagaactaa aaagtcaggc cgtatggtat tttttaggtc catttagagc
     4501 tttagactat tcatttagag ctaattatga atcgataacc gggttttggt atggaagggc
     4561 aacaatgggt ggaattgata atttattaaa acttgtaatc cattatttag gaggaagtta
     4621 taccattgct catgatgaaa taggtcatct tttgcaaaat tcaaaaattt cagttgggaa
     4681 tggaatcatc tttaattatg catacactaa tataatattt cattatttgg attttggtta
     4741 cgttggcgtt cttttattcc catttttata tggctttatc gtacgatata gtatttataa
     4801 atttactcaa aaaccaacat tactgactct tggtattatg atatatttgg tctatatatc
     4861 aatgttcaat attttttcgt gggaatttca gaagccttcc tcaattattt atgttttgtt
     4921 gttgctattt tttcaatatg tttattctca taaattcgta atattaaaat taaatggaaa
     4981 aaaaaaaggt ttatgtcggc atgagtgctg atattattca tccaggtcat ttgaatatca
     5041 ttcatgaggc ttcaaaattg gggcatgtaa cggttggtgt attaacagat tcagctattg
     5101 ccagttacaa gcgtttgcct tacttgaatt ttgatcagcg ctcattgata gttctgaatt
     5161 tgaagggtgt tgatgaagta atacctcaaa ctactcttga ttatgttccg aatttggaat
     5221 tgattaggcc tgactttgta gtacatggtg atgactggaa agagggtatt caacaagaaa
     5281 ctcgccagcg tgtgattgat actatctcta aatggggtgg aaaagtgata gatgttcctt
     5341 atacgaaagg tatttcttcc actttattga atactaggtt gaaggaaata ggaaccactc
     5401 ccgaaattcg tttgaagcga cttaaacgct taattgctgc taaaagtatt gttcgtatct
     5461 gtgaatctca tagtgggtta acaggtttga taatagaaaa taccagtgta gaagtcaacg
     5521 gagttaagcg tgaatttgat gggatgtggt ccagtagtct tactgattcg acttctaaag
     5581 gtaagcctga tattgaagcc gttgacttga caactcgtct tcatgatttg aatgatgctt
     5641 tggagtgtac taccaaacca gtaatttttg atggtgatac aggtggtaaa atagaacatt
     5701 ttgtatttac tgttcgcacg ctggaacgtt tagggatatc agctgttatt atcgaggata
     5761 aaataggtct gaagaagaac tcgctttttg gaacagacgc tgttcagacc caggatacaa
     5821 ttgaagggtt ttgtgataaa attcaagcgg gtaaacgtgc tcagataacg gatgatttta
     5881 tgataatagc tcgtattgaa agttttattg ctggaaaagg tttagatgat gctttaggac
     5941 gtgcattagc atatattaaa gcaggtgcgg atgggattat gatccatagc aaagataagt
     6001 ctggtgagga tataaagaac ttctgtcttg ctttacgcaa aatacataaa tccattccga
     6061 tagtggttgt tccaactact tataaccata tatcagaaga tgaattggct tcttggggag
     6121 taaatgttgt aatatatgca aatcatatgc ttagaagtgc atatcccgct atgcttaata
     6181 cggcgaagtc cattcttgcc aatgcacgct cttatgaagc caatgactta tgcatgccta
     6241 ttaaagaaat tctggaattg attccaggga ctaagtaaat agactcttta aaattaaatt
     6301 cttattatta tgatttctcc taatattttt gtagactctc ttggcgctat gcagatggat
     6361 ttttttactg gagttcccga ttctttattg aagaatatat gtgcatattt aacagatcac
     6421 cttgcttctg aacataacat tattgccgct aatgaaggtg ctgctatagg tttggctaca
     6481 ggatattatt tggctacgag gaaaattcca gttgtttata tgcagaattc aggtcttgga
     6541 aatgcagtaa atcctttaat gtcattgacg gataaagaag tttataatat tccattgtta
     6601 ttgctaattg gttggcgtgg tgaacctggt gtgaaagatg agccacaaca tattaagcag
     6661 ggaaaagtca cacttccatt acttgaggca atgggtatca gatatgctgt tttatctcaa
     6721 gatgaaaaga agtttatatc tcaacttaat gttgcaaaat cttacatgga tgagacaaga
     6781 gagccttttg cttttgtaat tcagaaagac actttcttag agtatagttc tgaaaattgt
     6841 gaagatgaac ggttgtcttt gaaacgcgaa gctgctattc atttcatagc agcttcatta
     6901 acagaaaaag atattataat atcgacaaca ggtatgatat cacgtgaatt atttgaatat
     6961 cgtgaatcta cttctttaga acatgagaga gattttttga ctgtaggttc tatggggcac
     7021 gcgtcgcaga tagcattggg aatagctctt aaaaagagag actgtaaagt ttattgcttt
     7081 gatggagatg gttccacttt gatgcatatg ggttcgttag caattgtcgg ttctttacat
     7141 ccggaaaact atgtacacgt agtctttaat aatggcgctc acgattctgt ggggggacaa
     7201 ccaacagtcg ctttagatat tgatttatgt aaaattgctc aagcttgtgg ttatgaaata
     7261 gtattgtctg tttctgataa cgaaggttta tgtgaagctc ttaaaaagat acagcaaata
     7321 cgcggcccta ttttgttgga agtaaaggtt cgaaagggag ctcgtaagaa tttgggaaga
     7381 ccagccaaca ctcctataca aaataaagag gcattcatgc ttttcttaga taatttatag
     7441 agtaattatg gaacagcgtt tttttttagg agagtcttct ataaaagaat tgattcctat
     7501 cttgcaagaa tatactcctg atcatatatt tcttgtaaga ggaaaaaagt cttatgagaa
     7561 atgtggtgca caggctgtgt tagaatctgt ttttgttaaa ttagcttgtg gcgtgacgga
     7621 attctttgat tttgaagcaa atcctaaaat tgaagatatg gaaaaaggac tttcattatt
     7681 gaaagaatat ccgacaaata tgattattgc tataggtgga ggtagtgtat tagatatggc
     7741 aaaattgctt cgtttttttt attcctattc tggtaatcct acgggggggg agtttgaaaa
     7801 gaagcgacaa ttaattccat taataacact tcctactaca gctggtacag gtagtgaagc
     7861 aactcatttt tctgttttat ataaagataa agtaaaatat tcagtagaac atgatgctat
     7921 tcttccggat gtagcgattg tatatccaaa atttacttat ggaaattcaa aatatctaac
     7981 atcttgtaca ggctttgatg ctctagcgca ggcgatagaa gcttattgga atgtgaatgc
     8041 tacggaagag tcggataaat atgcggcgaa agcaattaaa ctcttatggc ctaatttgcc
     8101 attggttgta aattctccta cagcggaggc taggaataag gtttcggaag gctcttattg
     8161 ggctggtcgt gcaattaata taactaagac tacagctcca catgcttttt cgtattcatt
     8221 tacaactgat tatggatatc cgcatggaca tgcagtagca ttaacatttc ccttttttat
     8281 gagatataat attggaaatt cggatatctt attgaatcct aatataaatc cttacttata
     8341 taagattaaa atgcaaaaaa tgttacagtg gttaaatatg gaatgcgtag atgttgcaat
     8401 cgctatgaaa gagtatataa gaaacttggg attagatatt gacatatctc aaattgacat
     8461 atcgaaaatc gtatcttctg ttaatattca aagattaata aacaatccat ataaagttga
     8521 agcgatatgt ctagaggaat aagtgaaaag gagcaacgat ggttgttagt attatcacaa
     8581 gttattgaag ttcttgataa aaataattta ttatatttta ttgatactgg tactctttta
     8641 ggagctgtta gagataaaat gtttattcct tgggataatg atattgattt agcagttatt
     8701 ggaggagata catctactac taatttgctt aaagtgtcaa aattgttata tgctttaggc
     8761 ttttacgtca ctttgacaaa gaaagaaatt gctatctgtt ctagagacgg ggtaaattta
     8821 ggaattaaat tatataaaaa gcaagcaagt cattatgaag catgtatggg aaaagtttat
     8881 agacatagac tattttattc tataaattta tacctctctg gagaggtcct gtataaaaaa
     8941 ggcttttctt ttaagtataa agctatctca ctctttgtga agtttagtca agtctttaag
     9001 ttaaaattgt cttgtatttg gcaaatgaag ttattgtttt gggctaagta tgatgaaaag
     9061 acggtgatta ttccttttga gtttgtagat gcaattattg attataattt ttataatata
     9121 attgtgaaag tgccaaataa gccagaaaga tatctagcat atagatatgg tgaaaattgg
     9181 aaggttccca aacaagacta tgattatatg attgatgaca agtctattat aaatattgat
     9241 taaaaaaata tgaaaacaaa tttcaacata aggagtatta ttgtaattaa taaaattata
     9301 atcaactttt tctttagaca atatagatgg tatttataaa tagcactttc atttatacac
     9361 caaaattggg cacttatata ttatattctc ggaacaaatg aacattctaa taaattgctc
     9421 caatctcaag aaaggtggag ggcttcaagt cgctgattcg gtttgttctt gtttgaatac
     9481 aattccccaa catgattttt ttttagtatt atcctcatgt atggatgata ctgctgatag
     9541 ggtatcggaa tacaaaaatg tcactgtagt gcgtcatgat atacgaaaca gttggtatac
     9601 attgttttgg ggacgtgata aggctatgga taatttggta accagacaca atattggtgg
     9661 agttcttacc atattcggac ctgcaagatg gaagccgaaa tgtaagcatc tatgtggatt
     9721 tgccagatcc catctcgttt tgaacgattc tccctatttt caacgtatgt cttggaagga
     9781 actaatgata gaaaaggcga gaaatcttta tttatattat tacttcaaac gtggtgtgac
     9841 tgctttttgg actgagaatc cttatatttc agagaaagta aagcacttgt tccctaccat
     9901 aaaagcctat actgtatcga attattataa tcaggttttc gatgatgaaa atactcaaat
     9961 agaacatgcc ttaccgtctt ttgacggttg tactttactg tccataaatg ctccttatcc
    10021 tcataagaat ttagcaattg ctatcgaagt cgctcgatat cttaaaaaac attattccga
    10081 atttagtttt cgattcgttt ttacggtgac agagaatgac ttgccaccta ttccttctta
    10141 tttgcgagag catttctgtc tgattgataa agttagtgta gcagaatgcc cttccttata
    10201 tcgtcaggca gatataatgt ttcaaccaac tttacttgaa tgtttcactg ccacatatgt
    10261 cgaagcgatg cgaatgcaag tgccaatttt aactacagat ttgccatttg ctcatggatt
    10321 atgtggaaat gcagctgtgt actattcgcc gttagatgca gaagactgtg ccaaatcaat
    10381 ttataaaatg gctgtagaca catcctttca agattatctg gtcggagaag gaataaaaca
    10441 acttaagtcc ttcgataatt ataatcggag agcagaaaaa ttaatcgatt tgatatcgaa
    10501 tttggatgat taatacatat tatatttatg tcagtattta aagacaaagt cctccttata
    10561 accggaggta ccggcagttt tggtaacgct gtattgcgtc gtttccttga ttctgatatc
    10621 aaggagattc gtatttttag tcgtgacgag aaaaagcagg atgacatgcg tcatcacctg
    10681 caaaatccca aagtaaaatt ttacattggt aatgtccgtg accgccagtc tgtagacggt
    10741 gccatgatgg gagtggatta tgtatttgcc gctgccgcct tgaagcaggt tcccagttgc
    10801 gagttcttcc cgttgcaggc tgtgcagacc aatgtgatcg gtacggacaa tgtactggaa
    10861 tctgccatag cgcatggtgt aaagaatgtg gtggtgcttt ctaccgacaa ggccgcctat
    10921 cccatcaatg ccatgggtat cagcaaggcc atgatggaga aggttgccat agccaagggc
    10981 cgtgcgttgg gtgaggaagc cggtacgacc atctgctgta cccgttacgg caatgtgatg
    11041 gcaagccgcg gttctgtaat tcccttgtgg gtggagcaga taaagaacgg caggcccatt
    11101 acggtaacgg accccaacat gacccgcttc atgatgacgc tggacgatgc cgtggatctg
    11161 gtaatctatg cgtttgagca cggccataac ggtgacctgt tcgtgcagaa ggctcctgcc
    11221 gccaccttgg atgtactggc gcaggccttg aaggaaacat acgcgcaggt tgatccgaaa
    11281 tacggtgaaa cggaggtgaa gaccatcggc acgcgccacg gcgagaaact gtatgagaca
    11341 ctggtaaccc gcgaagagat ggcaaaggct gtcgacatgg gcgactacta ccgcatccct
    11401 tgcgacaccc gtgacctgaa ctatgacaag ttcttcacgg aaggcaatga ggatattgcc
    11461 cgtatagagg actatcacag ccataacacc gcccgcctgg atgtggaggg catgaaaaag
    11521 cttcttttga aactccgctt catccgcgaa gacctcggga tggaggaaaa ggcgaagagt
    11581 gcggaaatca agagtgaata agtctttaat ttgaataata caacaggaaa gaaagaggga
    11641 atgtattgct cctttagaaa aaaaaactat ctttgccata ttgaaactaa taattgagaa
    11701 tatggcagca acagtattaa attcggtcca gcaacatctt ttgaagatgt ttgcttttga
    11761 tggttcggag gagcgattga atgaagttaa agaagtgttg accaaatatt tttctcagaa
    11821 gttagatgac cgcttgaatg acttatggga ttcgggagtt ttaaaccaag aaaaacttga
    11881 tgaactgaga aaaattgatg tccgcacact tccgaagaga tgaataggat tgtattagat
    11941 acaaatagcc tgatacagtg catttctccc aaaagcaggt accgtaagat ttgggattgc
    12001 tttctggatg gaacgaatca gttatgcgtt tctgatgaaa ttctaaatga atatgaggaa
    12061 atattagaac gtttggcagg tgtggaaata gctaagctgg tgattgatgt cattctcaat
    12121 agcccttata ctttgttctt tactccttat tataacttca atctgataaa atcagatccg
    12181 gatgataata agtttgtgga ctgtgcagtt gtggctaatg ccaagtttat tgttacggaa
    12241 gatcatcatt ttcatatatt gaagcaatat aatttcccaa aagtaagttt tataggtttg
    12301 gatgatttct gtcattcact atgaatatct tctcttttct gtgacaaaca gaacccatta
    12361 gtttatgaaa atcttagtga ccggcgcccg agggttcgtc ggccggaacc ttgtatccca
    12421 gctgcacaac atccgtgacg ggaaagcccg caattacggc attgcaagtg aggaacttgc
    12481 catttacgag tatgacatgg acagtgaccc tgccgaactg gatgtctatt gcaggcaggc
    12541 tgactttgtc ttcaatctgg cgggcgtgaa ccgtccccag gatgcgtctg agtttatgaa
    12601 agggaacttc ggcttcgcct ccacgctgct cgacacattg aggaaacacg gcaatacctg
    12661 tccggtaatg atctcctctt ccacgcaggc cgcactcgac aacccttacg gtgaatcgaa
    12721 gcgggcaggg gagcaactgt tgttcgaata ctccaaggaa acgggggcga aggtactggt
    12781 gtaccgcttc ccgaatgttt tcggcaaatg gtgccgcccc aactataaca gcgcggtggc
    12841 cactttctgc cataacatcg cccatgacca gcccatccgg gtgaacgacc cttcggtggt
    12901 gatgaacctg gtttacatag acgatgtggt ggacgagctt gtctctgcca tgggcggcag
    12961 ggaacaccgc aacggtgatt attgcgaagt gcccgtagta cataccatta ccttgggcgg
    13021 cattgtggaa ctgataaggt ctttcagaca aatgcccggt accttgtccg tcccggattt
    13081 gagcgatgcc tttacaaaga aactctattc cacatacctc tcatacctgc ctgaagaacg
    13141 gttcagctat cccctgaaaa tgaatgtgga cgaccgcggc agttttacgg agattatccg
    13201 tacttccgac cgcgggcagt tctcggtgaa catctccaag ccgggcgtta cgaaaggcca
    13261 gcactggcac cataccaaga acgagaagtt tgtggtggtg agcgggcacg gtctgataca
    13321 gctccgcaag ataggtacgg aggaggttgt cagcttcgag gtaaacggcg ggaggatgga
    13381 ggtcgtggag atgattcccg gctatacgca caacattgtc aacctttcgg aaacggaaga
    13441 cctggtgacc ttcatgtggt gcaacgagtg tttcgacccg gcacgtccgg acacttattt
    13501 tgaaaaagtc tgaaacgagt taaagaatat aggaacagat gg
//
