LOCUS       c00025_gnlPro..         5650 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JHMCPDBA_25.
ACCESSION   c00025_gnlPro..
VERSION     c00025_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 11:07:32
            Original ID  :: gnl|Prokka|JHMCPDBA_25
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5650
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5650
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1950:5440]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1951..5440
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..5650
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5650
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             393..626
                     /locus_tag="ctg25_1"
                     /transl_table=11
                     /translation="MNLQKQQLLLRLFRYFKSRKGEPRFFNKRNICLLAAAGAYILTPD
                     EFLFDGIGRLGLLDDAAVILGAVLTVFFPDGD"
     CDS             complement(660..1445)
                     /locus_tag="ctg25_2"
                     /transl_table=11
                     /translation="MVCRTTDEYRAMAQAGCLAVGEPAFWAGYDRSSADGFRDYFVQLT
                     ECEPARAAKFGMDHYTWLCINPKEAQDVAFARDVMKLIPEFIDRPNVLGIGEIGLNRNT
                     SHEMTIFEEHLDLAVRLNQLVLIHTPHLEDKLKGTVMILSALKNRPDLDPCRVLVDHCE
                     EHTFPLVKEAGYWAGLTLYPTSKLNPKRAADILELYGMDRVWANSAADWGDSDPLALTA
                     LACELRRRGFSRQETERLLLENPETFMGQSPKFRKVSSR"
     CDS             complement(1478..1750)
                     /locus_tag="ctg25_3"
                     /transl_table=11
                     /translation="MKRSKKEVLDADFAAARAGVLDVAAFLDRVDRGEGEADFRYHALM
                     DVLPLLSASGPGRVRAILEALSYRDDTVPETAAAKAACGAPNLSK"
     CDS             1951..2652
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg25_4"
                     /sec_met_domain="SucD_FumR (E-value: 2.5e-14, bitscore:
                     42.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNALVKTICTFVTSSIGRKIIVALTGLCLVLFLAGHLAGNLLIFG
                     GPEWINTYAHGLHSMPEAALWGIRAGLAVIFIIHVWLTIQLKLENHAAREPYVFKNTIK
                     ATLSSRYMIYTGLTVLVFLIYHLYQYTLRVGYDPAQFTTFISDGTVETFDVYKMIVTGF
                     SNVWCSAFYILAVLMLFSHLRHGVQSIFQTVGVDSRKIRPFYNFIAIAYGAIICLGFIS
                     VPVSVLLGIIK"
     CDS             2674..4632
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 176.1; E-value:
                     4.2e-54)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg25_5"
                     /sec_met_domain="FAD_binding_2 (E-value: 4e-55, bitscore:
                     176.1, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 1.1e-38,
                     bitscore: 120.8, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINFPVSDWMPDAKIPSGPIQDKWSKYKLESKLINPNNKRKYTVL
                     VVGSGLAGASAAATLAELGYNVKCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRL
                     FYDTVKGGDFRAREANVYRLAEVSSNIIDQCVAQGVPFGREYGGLLDNRSFGGAQLKRT
                     FYARGQTGQQLLLGAYQALEKEVAKGHIEMFSRHEMMDLVLVDGEAKGIVTRNLVTGEI
                     KSYAGDTVLLCTGGYGNVFFLSTNAMGCNVTAAYKAYKRGAFFANPCFTQIHPTCIPVK
                     GDYQSKLTLMSESLRNSGRIWVPKSRETAEKIRKKEIKPSDVPEEERDYYLERKYPSFG
                     NLAPRDISSRAAKEACDDGRGVAVTGKGVFLDFADEIKRMGREWIDGQYGNLFDMYEEI
                     TDDDPHETPMMIYPASHYTMGGLWVDYNLMSNVKGLHVLGEANFSDHGANRLGASALMQ
                     GLSDGYFVIPATLPAYLTTTKPGAVTTAAPEFKAAEDEVKERINKLLNVKGTKTVDSFH
                     RELGLIMWEDCGMARTEESLTHAIERIPQLRKEFWENVRVTGTADGINAELEKACRVAD
                     FLEFAQLLCYDALNRQESCGAHFRVEYQLPDGEAKRNDDEYAYVAAWEYTGVDSLPVLH
                     KEPLTFENVHLAIRSYK"
     CDS             4673..5440
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052:Succinate dehydrogenase-2Fe-2S iron-sulfur
                     cluster binding domain (Score: -1.5; E-value: 8.6)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg25_6"
                     /sec_met_domain="Fer2_3 (E-value: 2.1e-24, bitscore: 74.4,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 7.2e-12, bitscore: 34.5,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 4.1e-07, bitscore: 19.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTLNLTLKVWRQENREAKGRIETYAAKDIPVDASFLEMLDIVN
                     EGLVKEGKEPIHFDHDCREGICGMCSLTINGIPHGPERQVTTCQLHMRKFKDGDTVWIE
                     PFRAKAFPILRDLMVDRSALDRIIAAGGYIDVRTGSAPNANNIAVEKVKADAAFDAAAC
                     IGCGACVASCKNASAMLFTSAKIAHLNLLPQGQPERSKRVLAMMKQMEAEGFGNCTNQY
                     ECEAACPKGISVDNIARLNRDFMRANAKEGLCF"
ORIGIN
        1 ctcacaaaat cgtgagccgg ccttttcctt atccgttcaa acatctgccg aaaaaacaga
       61 ggggaaaagc ttccatttgc tgcggcaacc tcacggccag cacttgacgg aaaaatagca
      121 ttcagtctcc cccggtaggg tcatttcaag agcttccggc cccgccccac ttccgtacac
      181 gccccttttt cccatgccgg gacagccgga accggagcat gcaaagcccg gcttccctca
      241 gcatccggaa cagcccatcc tttcagcaat ccatttcccc gcacggtccg gatcgctgcg
      301 gttccgtccg tcggcaacag actccttccc cgaaacaggg aaaaggagtg ggaaaatggc
      361 tgagcttgaa cctttatccc ccgttcccgt ccatgaacct tcaaaaacag cagctgctac
      421 tgcgcctgtt ccggtatttc aaaagcagaa aaggagaacc gcgttttttc aacaaacgga
      481 atatatgcct tctggccgcc gccggcgcct acatcctgac tcccgacgaa ttcctgtttg
      541 acggaatcgg caggctgggc cttctggatg acgcggcggt catcctgggc gccgtcctca
      601 ccgtcttttt cccggacggg gactagaggg ccgtaccctc cgccgcgcct cgggcggaat
      661 tagcgggacg acactttccg gaacttcggg gactgtccca tgaatgtctc cggattttcc
      721 aggagaagac gttccgtttc ctgccgggaa aaaccgcgcc tgcgcagctc gcaggccagg
      781 gccgtcagcg ccaggggatc ggaatccccc cagtccgccg cggaattggc ccagacacgg
      841 tccattccgt acaattccag aatatccgcc gcccgcttgg gattcagctt actggtggga
      901 taaagggtca gcccggccca gtaaccggcc tccttcacca gcgggaaggt atgttcctca
      961 cagtgatcca ccagcacgcg gcaagggtcc agatccggac ggtttttcag ggcggaaaga
     1021 atcattacgg tccccttaag cttgtcttcc agatggggag tgtgaatcag cacaagctgg
     1081 ttgaggcgca ccgccagatc cagatgctct tcaaaaatag tcatttcatg ggaagtattc
     1141 cggttcagcc ctatttcccc gatgccgagg acattgggcc ggtcaatgaa ctccggaatc
     1201 agtttcatca catcccgcgc gaaagccacg tcctgcgcct ccttgggatt gatgcacagc
     1261 catgtgtaat ggtccatgcc gaacttggcc gccctggcgg gttcgcattc cgtaagctgt
     1321 acgaaataat cccggaaacc gtccgcggac gaacggtcgt aaccggccca gaacgccggt
     1381 tcccctaccg ccaggcagcc ggcctgcgcc atggcccggt attcgtccgt ggtgcggcac
     1441 accatatgta tatgaggttc tatgaaagcc atcattctta tttgctgagg ttcggggctc
     1501 cgcaggccgc tttggctgcg gcggtttccg gcacggtgtc atccctgtag gaaagggctt
     1561 ccagaatggc ccggacgcgt ccggggccgg atgcggacaa gaggggaaga acatccatca
     1621 gggcatggta gcggaaatcc gcctcccctt ccccgcggtc cacccggtcc agaaatgcgg
     1681 ccacatccag aacgccggcg cgcgccgctg cgaaatccgc gtccagcacc tcctttttac
     1741 ttcttttcat gcacagacga tacgcctatg cggaaagcct cgtcaagccg gaacaggcaa
     1801 caaaatgata accattccaa actgataaac agcaacctct ttccccggtt tttcaaaaat
     1861 ccgaaaagcg ccggaaattc aagaagaggc ttgccatccg ggcttgaacc gggcatcctg
     1921 tgaagacgca tttatttcat tagagcgcct atgaatgcac tagtaaaaac catatgtaca
     1981 ttcgtgacat cctccatcgg ccgcaaaatc attgtggctc tgacggggtt atgtttggtc
     2041 ttgttccttg cgggacacct ggcaggcaac cttctcatct tcggaggccc tgaatggatc
     2101 aacacctacg cccacggcct gcactccatg ccggaagcag ccctctgggg aatccgcgcg
     2161 gggcttgcgg tgatcttcat catccacgtc tggctgacca tccagctgaa gctggaaaac
     2221 catgcggccc gcgaacccta cgtgttcaag aacaccatta aggcaacgct ctcctcccgc
     2281 tacatgatct ataccggttt gaccgtcctg gtgttcctga tctaccacct gtaccagtac
     2341 accctgcgcg tgggatacga ccccgcccag ttcaccacct ttatttccga cggtacggtg
     2401 gaaacctttg acgtgtacaa gatgatcgtg accggattct ccaacgtatg gtgctccgcc
     2461 ttctacatcc tggccgttct gatgctgttc agccatctac gccacggggt acagtccatc
     2521 ttccagactg tgggcgtcga ttcccgcaaa atccggccct tctacaactt tatcgccatc
     2581 gcctacggcg cgattatctg cctgggcttc atctccgtgc cggtctcggt tcttctgggt
     2641 atcatcaaat aaccatcaac tcctctcaaa gtcatgatta attttcccgt aagcgactgg
     2701 atgccggatg ccaaaattcc ctccggccct attcaggaca aatggtccaa gtacaaactt
     2761 gagtccaaac tgatcaatcc gaacaacaag cgcaagtaca cggtgcttgt ggtaggttcc
     2821 ggcctggctg gcgcctccgc tgccgccacg cttgccgaac ttggctacaa cgtcaaatgc
     2881 ttctgctacc aggacagccc ccgccgcgcc cactccattg cggcccaggg cggcattaac
     2941 gcagccaaga actaccagaa cgacggcgac tccgtttacc gcctgtttta cgacaccgtt
     3001 aaaggcggcg acttccgcgc ccgtgaagcc aatgtctacc gtctggcgga agtctcctcc
     3061 aacatcattg accagtgtgt ggcccagggc gttcccttcg gccgtgaata cggcggcctg
     3121 ctggacaacc gctccttcgg cggcgcccag ctcaagcgaa ccttctacgc acgcggacag
     3181 acgggccagc agctcctgct gggtgcctac caggccctgg aaaaggaagt ggccaaggga
     3241 cacattgaaa tgttctcccg ccatgaaatg atggatctgg tgctggtgga cggcgaagcc
     3301 aagggcatcg tcacccgcaa tctggttacc ggcgaaatca aatcctacgc aggggatacc
     3361 gtcctgctct gcacgggcgg ctacggcaac gtgttcttcc tgtccaccaa cgcgatgggc
     3421 tgcaacgtca ctgccgccta caaggcctac aagcgcggtg ccttcttcgc caacccctgc
     3481 ttcacgcaaa tccaccccac ctgcattccg gtcaagggcg actaccagtc caaactgacg
     3541 ctgatgtcgg aatccctccg caacagtggc cgcatctggg tgcccaagtc ccgtgaaacc
     3601 gcagaaaaaa tccgtaaaaa ggaaatcaag ccttccgatg ttcccgaaga agaacgggac
     3661 tactacctgg aacgcaagta tccctccttc ggcaacctgg ctccgcgcga catttcctcc
     3721 cgcgccgcca aggaagcctg cgacgacggc cgcggcgtgg ccgtcaccgg caagggcgtc
     3781 ttcctggatt ttgcggatga aatcaagcgc atgggccgcg aatggattga cggccaatac
     3841 ggcaatctgt ttgacatgta cgaagaaatc acggacgacg acccccatga aacgcccatg
     3901 atgatctacc ccgcctccca ctacaccatg ggcggcctgt gggtggacta caacctcatg
     3961 tccaacgtga aggggctgca cgtcctcggt gaagccaact tctccgacca cggcgccaac
     4021 cgcctgggag cctccgccct gatgcagggc ctgtctgacg gctacttcgt cattcccgcc
     4081 acgttgcccg cttacctgac gaccaccaag cccggcgccg tcaccaccgc cgccccggaa
     4141 ttcaaggctg cggaagacga agtgaaggaa cgcatcaaca agctcctgaa tgtgaagggc
     4201 accaagacgg tggactcctt ccaccgggaa ctgggcctca tcatgtggga agactgcggc
     4261 atggcccgca cggaagaaag cctgacccac gccattgaac gcattcccca gctccgcaag
     4321 gaattctggg aaaacgtgcg cgtaaccggc acggcggacg gcatcaacgc ggaactggaa
     4381 aaagcctgcc gcgtggccga cttcctggaa ttcgcccagc tgctctgcta tgacgccctg
     4441 aaccgccagg aatcctgcgg cgcccacttc cgcgtggaat accagctgcc ggacggcgaa
     4501 gccaagcgca acgacgatga atacgcctat gtagccgcct gggaatacac tggcgtggac
     4561 agcctgcctg ttctccacaa ggaaccgctg accttcgaga acgtgcacct tgccatccgc
     4621 agctacaagt aaagatagcc aatacattaa cactcaaaag aaagattata acatggctaa
     4681 aacactcaat ctcactctca aggtctggcg ccaggaaaac cgggaagcca agggccgcat
     4741 tgagacctat gccgccaagg acattccggt ggacgcctcc ttcctggaaa tgctggacat
     4801 cgtcaatgaa ggcctggtca aggaaggcaa ggaacccatc cattttgacc acgactgccg
     4861 ggaaggcatc tgcggcatgt gctccctgac catcaacggc attccccacg ggccggaacg
     4921 ccaggtgacc acttgccagc ttcacatgcg caagttcaag gacggcgata ccgtctggat
     4981 tgaacccttc cgcgcgaagg ctttcccaat cctgcgggac ctgatggtgg accgctccgc
     5041 cctggaccgc atcatcgccg ccggaggcta tattgacgtg cgtaccggtt ccgcgccgaa
     5101 cgccaacaac atcgccgtgg aaaaagtgaa ggcggacgcc gcctttgacg cggccgcctg
     5161 catcggctgc ggcgcctgcg tggcctcctg taaaaacgct tccgccatgc tgttcacctc
     5221 cgccaagatc gcccatctca atctgctgcc gcagggccag ccggaacgtt ccaagcgcgt
     5281 cctggccatg atgaagcaga tggaagcgga aggctttggc aactgcacga accagtatga
     5341 atgtgaagcc gcctgcccga aaggcatcag cgtggacaac atcgcccgcc tcaaccgcga
     5401 cttcatgcgg gccaacgcca aggaaggcct ctgtttctaa agaagattcc ttcttaagac
     5461 gctctgtaaa aactcccggc ggcatcggct gtcgggagtt ttttcattac cagatttcct
     5521 tgaaccagga ataaaatacc agacagttgc ttcctcatcg tcgcgcagcg taaaaaattg
     5581 ttacaccgga aggcacccag taatccagga aaccgtactt ggaaatgccg gacgaaacct
     5641 aagcaggccc
//
