LOCUS       c00215_gnlPro..         7263 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MFBLIHAM_215.
ACCESSION   c00215_gnlPro..
VERSION     c00215_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 09:02:25
            Original ID  :: gnl|Prokka|MFBLIHAM_215
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7263
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7263
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[227:3713]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      228..3713
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..7263
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7263
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             complement(228..983)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg215_1"
                     /sec_met_domain="Fer2_3 (E-value: 3.4e-27, bitscore: 83.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 1.1e-12, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.2e-06, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDIKVTLKVWRQKSPKDKGQFETYQIDKINQSVSFLEMLDILN
                     ERLVSEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFNDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFVSVNTGGVPDANSIAIPRADAEMAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(1011..2954)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 195.7; E-value:
                     4.7e-60)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg215_2"
                     /sec_met_domain="FAD_binding_2 (E-value: 3.8e-61, bitscore:
                     195.7, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 3.8e-39,
                     bitscore: 122.1, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQIDSKIPQGPLAEKWKNYKDHQKLVNPANKRRLDVIVVGTGLA
                     GASAAASLAEMGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKG
                     GDYRAREANVYRLAEVSNSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQT
                     GQQLLLGAYSALSRMIGLNKVKMYTRHEMLDVVKVDGRARGIIARNLITGQIERFSAHA
                     VVVATGGYVNTFFLSTNAMASNGSAAWQCYKKGAYFANPCMVQIHPTCVPVKGDYQSKL
                     TLMSESLRNDGRIWVPKKLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDV
                     ASRAAKERCDAGFGVNNTGLAVFLDFSDAINRLGKDVVKAKYGNLFDMYEEITNDDPYE
                     TPMMIYPALHYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYF
                     VLPYTIQNYLSDQIQVPRFSTDLPEFAEAEKGIKDRIKKLMSIKGKESVDSIHKKLGHI
                     MWEHVGMARDAKGLQEAIQMLKELKKEFWSNVYIPGEADNLNVELDKALRLADFIEIGT
                     LMAHDALDRAESCGGHFRTEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNY
                     QFVVPQTRNYKK"
     CDS             complement(2988..3713)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg215_3"
                     /sec_met_domain="SucD_FumR (E-value: 8.7e-77, bitscore:
                     245.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWLLNSSIGKKLIMSVSGLFLVLFLLFHLSMNVAAAFSGEAYNMI
                     CSLLGANWYAVAATLVLAAGVVIHFVYAVLLTLQNRKARGNDRYAINARPKGVEWASQN
                     MLVLGIIIILFMVLHFSQFWYKMMFAELLGNHMVTLCDGTTTVSPQDGAAFINYYFQGN
                     ILNTVLYLVWYVALWFHLTHGFWSAIQTIGWSNTIWMNRWICISKIVATVICVVFAIIT
                     IVFYLNGVGGDYVAVCAGH"
     CDS             3966..5363
                     /locus_tag="ctg215_4"
                     /transl_table=11
                     /translation="MLQRKMLSFLFMMIFIPSVRAQWKDSTRFINKEHEFETTYNKISE
                     NKVYQMLRVPVPLFAISAITYGQGDKFRTLRNTHTPNFHYRYDDFLQYTPLLAVYGMKL
                     GGVESRSSWPRMIVSNAFSATIMAATVNSMKYTIKRERPDGSKNNSFPSGHTATAFMAA
                     TIMHREYGLTRSPLYSIGGYTVATATAFSRQLNNRHWLSDVLAGAGIGILSTELGYFLA
                     DLIFKDKGLNLPDKLRESAPKGGRPSFLEFAVGYSIINGSIEVGKNIRLSSPGATNVSV
                     KGGYFFNPYFGIGGEITALASPMAFDMSLYTGTTPSTKYDITRIHADPTGVFTFTAGPY
                     FSLPVASKLLLGTKFQAGYSYLVKNEVEMDLQEKHPPYGTINNIAFMKGNGSSNYVLRT
                     GISATGIVSRNLGIRMFVDYDYTFLKAPYEILTSLRDSQPVYGESHLSKHKIHYFTIGA
                     TVTALFW"
     CDS             complement(5367..7190)
                     /locus_tag="ctg215_5"
                     /transl_table=11
                     /translation="MNKTILLVVFCFSLFVSGYSQGIRFDSYPSHPRLLVKKGEEQKIK
                     SDLALNPEMLRLHNYLLGQCDDLITTPVLAYKKEGKRLLAVSREALRRIFYLSYAYRMT
                     GEDKYRLRAEQEMIAVCSFTDWNPSHFLDTGEMTMAVAIGYDWLFDRLSPDARRLVQEA
                     ILNKGFAPSRDKRYNWFLRATNNWNQVCNAGLVYGALAVLEDHPEEAREIIERALSTVG
                     LTLKGYGPDGAYPEGYNYWGYGTTFQVLLNAALESALGTDGGLSSAEGFLASARFMQFM
                     TGTTGKCFNFSDSRESVFALPAMYWFADKLQDPTLLWNEKDMLSRAELSFSDEESRFLP
                     LIPIYGSQYKMNELVPPAMKLWAANGQTPVVLVRTGWENGEGFYLGIKGGTASTSHAHM
                     DAGSFVFDASGVRWVQDLGMQEYYSLEKENVDLWNMSQNGQRWNVFRYNNLAHNTLTVN
                     GKYHDVNGFVPVTNIYSEAHKLGASLNMTDLFGGELENAVREVALINEKYLSVIDRVKA
                     GSQPATVRWTIVTGAIPRVLDSHMVELTKDGKCMQLIVDSPSVTSVSVLDNVSKNSYDA
                     SNEGTCRIVFDVSLESGQESDIKVRLVPAAD"
ORIGIN
        1 ttgttaacct taaatctaat actattatga aaaaaccaca gtacaaagat acaggtattc
       61 tccaacttgt caatagttaa aatgctaatt agtgttgtat agcatataag aaacactaac
      121 aacattcaag atatttatct atgacttcag aaatgttaaa ataggggcaa aaaagaagag
      181 gcgctgccat aagcaacgcc tctccggaca tacatatata atgtatatta gtcttgcaat
      241 ttagcacaaa tgaactcgcg gttcaaacga gcgatgttgc tgacagagat gttcttcgga
      301 cattccattt cgcaagcacg cgtgttggta cagttaccga agcccagttc gtccatcttg
      361 gcaaccattg ctttcgcacg acgagcagct tctaccttac cctgaggcaa cagagccaac
      421 tggctaacct ttgcagaaac gaacaacata gccgaaccgt tcttacaagc agcagcacaa
      481 gcaccgcaac cgatacatgc agcagcatcc atagccattt cagcatctgc acgaggaata
      541 gcgattgaat tggcatcagg tacaccacct gtgttcactg atacgaaacc acctgcctgg
      601 ataattttat cgtaagcata acggtctacc atcaagtcgc ggattaccgg gaaaccggca
      661 gaacgccacg gttcgattgt aatagtttcg ccatcattga agcgacgcat ataaagctgg
      721 caagtagttg cgccggttgc aggtccgtgc ggatgtccgt ttacatacaa agaacacata
      781 ccgcagatac cttcacgaca gtcgtgatcg aatacgatcg gctctttacc ttcactgacc
      841 aaacgttcat tcaaaatgtc taacatttcc aggaaagaaa cactctgatt gattttgtct
      901 atttggtaag tctcaaattg tcctttatct ttcggacttt tctgacgcca aactttaagt
      961 gttactttta tatctttatc catgattcta atttttgtga ttgatgatta ttacttcttg
     1021 tagttacgtg tttgaggaac aacgaactga tagttcaaat cttccttcag cataaccgga
     1081 tctttgtctt caccctggta ttcccaacaa gatacgtaca tgaacttatc gtcatgacgc
     1141 agggcttcgc cttcctcggt ctggtgctct gtacggaagt gtcctccaca agattcggca
     1201 cggtccaatg catcgtgtgc catcaatgta ccaatctcaa taaagtcagc caaacgcaat
     1261 gccttatcca actctacgtt caggttatcg gcttcacccg gaatatatac attactccag
     1321 aactctttct tcagctcttt caacatctgg atcgcttcct gcaaaccttt tgcatcacgt
     1381 gccataccta catgttccca catgatatga cccagtttct tatggataga atctaccgac
     1441 tctttaccct tgatactcat caatttcttg atgcggtctt tgatcccctt ttcagcttct
     1501 gcaaactcag gcaggtcggt gctgaaacgt ggaacctgga tctggtctga cagatagttc
     1561 tgaattgtat aaggcaatac aaagtagcca tcggccaatc cctgcatcaa cgcagaagca
     1621 cccagacggt tagctccgtg gtcagagaag ttagcttcac cgatagcgaa cagacccgga
     1681 atagaagtca tcaattcata gtcaacccac agaccaccca tcgagtagtg caatgcagga
     1741 taaatcatca tcggagtttc gtatggatcg tcgttggtaa tttcttcgta catgtcgaac
     1801 aagttaccat atttagcttt tacaacatct ttacccagac ggttgatggc gtcggagaag
     1861 tcgaggaaca cggccagacc tgtgttgttc acaccgaagc cggcgtcgca acgttcttta
     1921 gcggcacgtg aagccacgtc acgcggaacc aggttaccga atgccggata acggcgttcc
     1981 agatagtagt cgcggtctgc ttcaggaatg tctttccctt tcttagttcc ggcctgtaat
     2041 gcttttgcat cttccagttt cttcggaacc cagatacgac cgtcgttacg caatgattca
     2101 gacatcaaag tcagcttaga ctggtagtca cctttcaccg gtacacaagt cgggtggatc
     2161 tgcaccatac aagggttagc gaagtaagca cctttcttgt agcactgcca tgcagcagaa
     2221 ccgttggaag ccattgcgtt agtagacagg aagaaagtat ttacataacc acctgttgcc
     2281 accactactg cgtgagcaga aaaacgttcg atctgacctg tgatcaggtt acgtgcgatg
     2341 ataccgcggg cacggccatc caccttcaca acgtccagca tttcgtggcg ggtatacatc
     2401 tttactttgt tcaaaccgat catacggctc aaagcagagt aagcacccag caacaactgc
     2461 tgtccggtct gaccgcgggc atagaacgta cgggatacct gagcaccacc gaatgaacgg
     2521 ttatccagca gaccaccgta ttcgcgagcg aaaggaacac cctgtgctac gcactggtcg
     2581 ataatagagt tggaaacttc agccagacga taaacgtttg cttcgcgggc acgatagtca
     2641 ccacctttga ttgtatcgta gaacagacgg taaacagagt caccatcatt ctgataattc
     2701 tttgcagcat tgataccacc ctgtgcggcg attgagtgtg cgcgacgggg agaatcctgg
     2761 atacagaagt tcaatacgtt gaatcccatt tctgccagag aggcagcagc agaagcaccg
     2821 gccagaccag taccaactac aatcacgtcc agacgacgtt tgtttgcggg gttcaccagt
     2881 ttctgatgat ctttataatt tttccacttc tcagccaacg ggccctgagg gattttagaa
     2941 tctatttgag tcataatgat ttattttcaa tattgttaca ccttaaatta atgtcctgca
     3001 caaacagcta catagtcacc acctacacca ttcaggtaga aaacgatagt aatgatagca
     3061 aacactacgc aaataacggt tgctacaatc ttggaaatgc agatccaacg gttcatccag
     3121 atcgtgttac tccaaccgat agtctgaata gcgctccaga acccgtgagt caggtggaac
     3181 cacaaagcta cgtaccatac cagatacagc actgtattca ggatattacc ctggaaatag
     3241 tagttgataa acgctgcacc gtcctgagga gatacggttg ttgttccgtc gcaaagtgtt
     3301 accatgtgat tgcctaggag ttcggcgaac atcatcttat accagaactg gctgaagtgc
     3361 aacaccatga acaggataat gataataccc agtaccagca tgttctgaga tgcccattcc
     3421 acacctttcg gacgagcgtt aattgcataa cggtcgttac cgcgagcctt acggttttgc
     3481 agtgtaagga gaacagcata cacaaagtga atcacaacgc ctgctgccag caccaatgtt
     3541 gctgccacgg cataccaatt tgctcccaac aatgaacaaa tcatgttata ggcttctccc
     3601 gagaaagcag ccgccacatt cattgacaag tggaacaata agaacaaaac aagaaaaagg
     3661 cctgatacac tcattatcag cttcttcccg atagaagaat taagtaacca cataagatnn
     3721 nnnnnnnnag ataataattt aagttattta attgttttta gaaattcata tcaatatact
     3781 caattatcag gcaaaggtat gctaaataca cgtatcacgc aaacgattaa agaaatattt
     3841 cgccaatcaa acctatacat ttcaggaggg acaaacggta aatcctattc aataaataaa
     3901 ttaacgaaaa tgtaaccagt actaaccggc atttttatat cttcgtggca taatcagggg
     3961 atcgtatgtt acaacgtaaa atgctgagct tcttatttat gatgattttt ataccgtctg
     4021 ttcgtgctca gtggaaagat agtacgagat tcataaataa agaacatgaa tttgaaacca
     4081 cttacaacaa aatatcagag aataaggtat accagatgct gcgtgtccct gttcctcttt
     4141 tcgccatttc agccataaca tatgggcagg gcgataaatt ccggacattg cgcaatacgc
     4201 atacgcccaa cttccattac agatacgatg attttctgca atatactccg ttgcttgctg
     4261 tttacgggat gaagctcggc ggtgtggaaa gtcgtagttc atggccccgg atgatcgtat
     4321 cgaacgcttt ttcggcaacg atcatggccg ctaccgtcaa cagcatgaaa tatacgatca
     4381 aacgcgaacg ccccgacggc tcaaagaaca actcattccc ttcgggacac acggcaaccg
     4441 ctttcatggc tgctaccatc atgcaccggg aatatggtct gacccgcagc ccgttatata
     4501 gtatcggggg atatacagtc gccacagcca ccgctttcag ccggcaactg aacaaccgcc
     4561 actggctgag cgacgtactg gccggggccg gtatcggcat cttatccaca gagttagggt
     4621 attttctggc agacctgatc ttcaaggaca aaggcttgaa tctccctgac aagctcaggg
     4681 agtctgcccc caaaggaggg cgcccttctt tcctggagtt cgccgtagga tacagtatca
     4741 tcaacggaag tatcgaggta ggtaaaaata tacgcctgag ctcgccggga gcaaccaatg
     4801 tcagcgtaaa aggcggttac ttcttcaatc cttatttcgg tatcggcggc gaaataacag
     4861 cgctggcaag cccgatggca ttcgacatgt cgctttatac aggaacaacc ccttcaacca
     4921 aatatgacat cacccgcatc catgccgatc cgaccggtgt ttttactttt acggcagggc
     4981 cttatttctc gttacctgta gccagtaaat tgcttttagg aacaaagttc caggccggat
     5041 attcctatct ggtgaaaaat gaagtggaaa tggacttaca ggaaaagcac cccccatacg
     5101 gaaccataaa taatattgct tttatgaaag gcaacggtag cagcaactat gttttacgca
     5161 caggcatttc agcgaccggg atcgtcagcc gcaacctggg tatccgtatg ttcgtagatt
     5221 acgattatac gttcctgaaa gcaccttacg aaatcttaac cagcctcaga gacagccaac
     5281 cagtctacgg cgagagccat ttaagcaagc acaaaataca ctattttaca ataggggcaa
     5341 ccgtaacggc gctgttctgg taattatcag tcagcagcag gaaccagccg gactttgata
     5401 tcgctctcct gcccgctttc cagacttaca tcgaaaacga tacggcaggt tccctcattg
     5461 gaagcatcat agctattctt cgatacatta tcaaggacgg acacagaagt tacggatgga
     5521 ctatcaacga tcagttgcat acatttgccg tcttttgtca gttccaccat atgactgtcc
     5581 aatacacggg gaatggcccc ggttacaatc gtccatctga cagttgccgg ctgactgcca
     5641 gcttttacac gatcaatcac cgacagatat ttttcattga tcagagcaac ttcacggacg
     5701 gcattttcca attctccgcc gaacaagtcc gtcatattca gagaagctcc gagtttatgc
     5761 gcttccgaat agatatttgt cacaggtaca aaaccgttta catcatggta tttaccgttt
     5821 acagtcagtg tattatgcgc caggttatta taacggaaca cattccagcg ctgcccgttc
     5881 tgcgacatat tccataaatc tacattttcc ttttccagcg aataatattc ctgcatcccc
     5941 aggtcctgca cccagcgaac acccgaagca tcgaacacaa acgatccggc atccatatgg
     6001 gcatggctgg tcgaagccgt tcctcctttt attcccagat agaaaccttc tccattctcc
     6061 caacccgtac ggaccagcac gacaggcgtc tgtccgttag ccgcccacaa tttcatagca
     6121 ggaggcacca gttcattcat tttatattgt gaaccataaa tcggaatcaa cggaaggaaa
     6181 cggctctcct catccgaaaa agacaactcc gcccgactca acatatcctt ttcattccaa
     6241 agcagtgtag ggtcttgcag cttgtcggca aaccagtaca tagccggcaa agcaaagaca
     6301 ctctcacggg aatcgctgaa gttaaagcat ttaccggtcg ttccggtcat aaactgcata
     6361 aaacgggcag aagccagaaa accttcagcc gaagagagac cgccgtcagt ccccaaggcg
     6421 gattccaacg cggcattcaa aagaacctgg aaggtagtcc cgtatcccca ataattatat
     6481 ccttcgggat aagcgccatc gggaccatag cccttcaaag tcaagccgac tgtagataaa
     6541 gcacgttcaa tgatttcacg ggcctcttcc ggatgatctt ctaaaacggc aagtgcaccg
     6601 taaaccagtc cggcattaca gacctggttc cagttattgg tcgctctcag aaaccagtta
     6661 tatcttttat ccctcgacgg ggcaaaacct ttattcagaa tcgcctcctg caccagcctc
     6721 cgggcatccg gagataaccg atcgaacaac cagtcataac cgattgcaac cgccatagtc
     6781 atttcgcccg tatccagaaa atgggacggg ttccaatcgg taaacgaaca cacggcaatc
     6841 atttcctgct ctgcccgcaa acgatactta tcctctcctg tcatccggta agcatatgaa
     6901 agatagaaga tacgccgcaa ggcttcacgc gatacggcca gcaaccgttt cccttctttt
     6961 ttatatgcca gcacaggcgt cgtgatcaga tcgtcgcatt gcccaagtag atagttatgc
     7021 aaccggagca tctccggatt aagagccaga tcggatttaa tcttttgctc ctctcctttc
     7081 tttaccagca accggggatg agaagggtaa gaatcaaagc gtattccctg tgaatacccc
     7141 gaaacgaaca gggaaaagca aaacacaaca agcaggatag ttttattcat acaggtatta
     7201 tatttaattg gagttacaaa cttacataaa aattgagatt tataagcgcg tctaataaaa
     7261 ata
//
