LOCUS       c00265_gnlPro..         5067 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CILBIEBH_265.
ACCESSION   c00265_gnlPro..
VERSION     c00265_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 11:23:08
            Original ID  :: gnl|Prokka|CILBIEBH_265
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5067
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5067
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:1856]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..1856
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5067
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5067
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..1114
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg265_1"
                     /sec_met_domain="Gly_radical (E-value: 1.4e-47, bitscore:
                     149.7, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 1.3e-137, bitscore:
                     448.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="EFCAKVSIDTSSIQYENDDLMREVRQSDDYGIACCVSYQEIGKQI
                     QFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTLKFEEVMDNYKKVLTEIAR
                     VYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGLSIALDSLSAIKYAKVTAR
                     RNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEELKKLPVYKNARPTLSLLTI
                     TSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDSQDGISN
                     TFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLNRDMLYDAMEHPENYPQLT
                     IRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             1128..1856
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.5;
                     E-value: 1.4e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg265_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.3e-29, bitscore:
                     92.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(1849..2298)
                     /locus_tag="ctg265_3"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDKDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(2285..2452)
                     /locus_tag="ctg265_4"
                     /transl_table=11
                     /translation="MKEIVSRILDVIMYLIPFFGKRKRDRVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             complement(2512..3015)
                     /locus_tag="ctg265_5"
                     /transl_table=11
                     /translation="MAMTVSYSVVPRKNPAKKSEPAKYYAQAQASGELDFEELCEAITS
                     RSTCTETDVRAAISGILYEVKRALKAGRIARLGDLGSLQIGLNSEGAASVKEFSGSMIK
                     GAHLIFRPGKTLAELMKILSYQQVQTRAVAQAGAGDGGGEDPDKNPDSGGDGSGDEEAP
                     DPTV"
     CDS             3209..3439
                     /locus_tag="ctg265_6"
                     /transl_table=11
                     /translation="MNYKEDSFLIRTYTKAELAHLYNPHVCLKVALQILRRWIIYNLPL
                     LHELEQEGYRARNRLLSPKQVATIIHFLGEP"
     CDS             3526..5067
                     /locus_tag="ctg265_7"
                     /transl_table=11
                     /translation="MKHFTIFQGFYYAIAEMTEEEIVSTIGSFTYREKVEEIRRIFAEQ
                     GEKAANEKKKELPAIAFSASYRGRRTKVNLVKYLGHIVIDIDHLSKEELARILPIIKRC
                     DYTRIAFISPKGMGVKIIVRACHPDETLPETLQEIEDFHHAAYTRLVSFYTELCQIEID
                     TSGQDVARTCLFSYDPEIYFNPNADAFLVDQPQASYKVSNRKNLSGSKQQTPPDGPPTN
                     EDTALNAHSANASLVLTLTYYHNKSEKYIVGNRNNYLHHLSCTFNRYGIPQEEASAFIK
                     SQFTDFPADETASLINSAYAHTDEFNTCKLNGTQKRILRIEQYISEHYETRYNEVLHIM
                     EYRRRHPDTEKPEPFRILDEMMENSIWIEINELGYACTVKTIENLIYSDFSQSYHPIRE
                     YFELLPEWDGTDYIRILADSVQTSHQEFWAECLERYLVAMCAAATQENIVNHTVLLLCS
                     EIQNIGKTTFINNLLPPELRAYLSTGLINSNNKDDLAKITQAMLINLDEFEGMSGR"
ORIGIN
        1 agaattctgt gcaaaggttt ctatcgacac ttcttctatc cagtacgaaa atgacgacct
       61 gatgcgcgaa gtacgtcagt ccgacgacta cggaatcgcc tgctgcgtat cataccagga
      121 aataggtaag cagattcagt tcttcggtgc ccgttgcaac ctggccaaag ccctgttgct
      181 tgccatcaac ggcggacgtt gcgagaatac cggtacagtc atggtgaaga acatccccgt
      241 actgaccagt gacacgctga agttcgaaga agtaatggac aactacaaaa aagtgctgac
      301 cgaaatcgcc cgcgtatata acgaagcgat gaacatcatc cactatatgc acgacaagta
      361 ttactacgag aaggcccaaa tggctcttgt agatacgaac ccgcgcatca acctagctta
      421 cggtgtagcc ggactttcca tcgccctcga ttcactgtct gccatcaaat atgcgaaagt
      481 aactgcccgc cgcaatgata tcggtctgac agaaggcttc gacatcgaag gagaattccc
      541 ttgcttcggt aacgacaacg acaaggtaga ccaccttggc gtagacctgg tatacttctt
      601 cagcgaagaa ttgaagaaat tgcctgttta caagaatgcc cgtcctaccc tctccttgct
      661 gactatcact tccaatgtga tgtacggcaa aaagaccggt gctactcccg acggacgtgc
      721 caaaggtgtt gccttcgctc cgggtgccaa cccgatgcac ggacgcgaca agaatggtgc
      781 gatcgcttct ctgagttctg tagcgaaact tcgttaccgt gactcacagg acggtatcag
      841 caacaccttc tctatcgttc cgaaatcact gggtgctacg gacgaagacc gtatcgagaa
      901 cctcgtaacg atgatggacg gttacttcac caaaggtgcc caccacctga acgtaaacgt
      961 actgaaccgt gatatgctgt acgatgccat ggagcatccg gaaaactatc cgcagctgac
     1021 tatccgcgtt tccggctatg ccgtaaactt cgtgaagctg agccgcgaac accaactgga
     1081 agttatcagc cgtagcttcc acgaacgtat gtaatctatt actctatatg atgataaacg
     1141 tacattcata cgaaagtatg ggaacattcg acgggccggg cttacggctc gtcgtttttc
     1201 ttcaaggatg caacttccgt tgtctctatt gcgccaatcc ggatacgata gcaggaaagg
     1261 gaggcacgcc taccccaccg gaagaaatcg tccgcatggc aatgagccag cgccccttct
     1321 tcgggaaacg aggaggaatc actttctccg gaggcgaacc gacgtttcag gcgaaagcac
     1381 tcgtcccgct ggtccgcgaa ctgaaagaaa aaggcatcca tgtctgtctg gacagcaacg
     1441 gaggactttg gaacgaagac gtagaagaat tattcagact gaccgatctt gtgttattgg
     1501 atataaagga attcaaccct aaccgccatc aaacgctcac cggaagaagc aacgagcaaa
     1561 ccatccgcac ggccgcctgg ctggaagaac aggagaaacc tttctggcta cgctacgtgc
     1621 tagtgcccgg atacagcgat tttgaagaag acatacgcgc actgggagaa gcactcggaa
     1681 aatacaagat gatccaacgg gtggaaatac tgccttacca tacactgggc gtacataagt
     1741 acgaagcgat gggacaggaa tacaagatga aaggggtaaa agaaaatacc cccgaacagc
     1801 ttgaaaaagc tgcggaggta ttcaaagagt atttcactac ggtggtagtt aattaatgga
     1861 gaattctcca atgacctcaa agcaagggca ttctttaatc cgttcccaag gatctacgat
     1921 cccattatag ttggtgtcgg gacttaaatc ccgatgccct actactttag cttccggata
     1981 acgctgcaac aattcgtgta ccaagcgatg tagcgcctcc ttctgacggg gtgtacgcgt
     2041 atccgcagct tttccattct catccagacc gccctcataa catacaccta agctgatcgc
     2101 attatagcca cgtgcatgag ctccgaccgt atctttatcc cgcatcggct caatctcgcc
     2161 cgacttgcgt atataataat gataccccca gcacgaaaag ccacgataac ggtgagccgt
     2221 atccacatcc tttgccgtaa agtcgcaatc gacacgggtg gcggaacaat ggatcacaat
     2281 cagactaatc tttctcatgt tccttttccc tttccgtcgt tttcactttg cacacctcct
     2341 tacaagtagt atgatagcgc acttctctca ccaccctgtc ccgctttctc tttccgaaga
     2401 aaggaatcag atacatgatc acatctaaaa tccgacttac tatttctttc atcttatcaa
     2461 aatattaatg gaagttattc taattcgtta ttaccttgac ccgtctgcca attataccgt
     2521 agggtccgga gcctcttcat caccggagcc atcaccaccg gaatccggat tcttgtcagg
     2581 gtcttctccc ccgccatcgc ctgcacccgc ctgcgccacc gcacgagtct gcacttgctg
     2641 ataactcaga atcttcatca actccgccag cgtcttaccg gggcggaaaa taagatgcgc
     2701 ccccttaatc attgaaccgg aaaattcctt gaccgatgcc gcaccttcac tgttgagtcc
     2761 tatctgcaaa ctgcccaaat cgcccagtct cgcaattctt cctgccttca acgcacgctt
     2821 cacttcgtaa agaatacccg aaatagccgc acgtacatcc gtttccgtac aagtagaacg
     2881 actggtgatc gcttcgcaca actcttcaaa gtccaactct cccgacgcct gcgcctgtgc
     2941 ataatacttt gccggttcgc tcttttttgc aggattcttg cgcggcacca ctgaataaga
     3001 tactgtcatt gccataataa aataatttta aagtttataa tacataggat gcatcccctt
     3061 cttgttaaca gcacaaagat aaggatagcc ggacaagcca tattccccaa cgaagtttct
     3121 tgattcccaa cgaataaaaa cgaagtcaaa cctttgaata acaaggaatt ttatgtaact
     3181 ttatagcata aaacaagaag aaaacatcat gaattacaaa gaagattctt tccttatccg
     3241 cacctatacc aaagcggaac tggctcacct ttacaatccc catgtctgcc tcaaagttgc
     3301 tttacaaata cttcgtcgtt ggattattta caacctccct ctcctgcacg aactcgaaca
     3361 ggaaggctac cgcgcacgca accgtctcct ttcgccgaaa caagtggcaa ctatcataca
     3421 ctttttgggc gaaccgtagt aacttattgc cattttatac aaagttcctt tacacatttc
     3481 cgcaaagaag ttcctttgca tcgacaaaca ataatcaaaa taaaaatgaa acatttcacc
     3541 atatttcaag gtttctacta cgcaatagca gagatgaccg aagaagaaat agtgtccact
     3601 atcggcagtt ttacctaccg ggaaaaagtc gaagaaatac gccgcatctt tgcagagcaa
     3661 ggagaaaagg ctgccaatga gaagaaaaaa gaattgccgg caatcgcctt ctccgccagc
     3721 taccgaggaa ggcgtaccaa agtgaacctc gtcaaatatc tgggacacat cgtgatagac
     3781 atcgaccacc tcagcaaaga agaattggca cgcatcctcc ctatcatcaa gagatgcgat
     3841 tacacccgca tcgcctttat cagccccaaa gggatgggcg tgaaaatcat agtacgcgca
     3901 tgccatccgg atgagacatt gcccgaaacc cttcaggaaa tagaagactt ccatcatgca
     3961 gcttacacca gactcgtttc tttttacacc gaactctgtc agatagaaat cgacacctcc
     4021 ggtcaggatg tggcacgcac ttgtctgttc tcttacgatc cggagattta tttcaatccg
     4081 aacgctgacg cttttcttgt agatcaacct caggcatcct acaaggtttc aaatcgtaaa
     4141 aatctctccg gcagtaaaca acagactcct ccggacggcc ccccgacgaa tgaagacaca
     4201 gcgctcaacg cccattccgc caacgcttct ctggtgctga ctttaaccta ctatcacaac
     4261 aagtccgaaa aatacatagt gggcaaccgg aacaattatc tgcaccatct ctcatgtacc
     4321 ttcaatcgct acggcattcc gcaggaggaa gcatccgcct ttatcaaaag ccagtttaca
     4381 gactttccgg cggacgagac agcctctttg atcaatagcg cctacgctca taccgacgaa
     4441 tttaatacct gtaagctaaa tggcacgcag aaacgaatat tacggataga gcaatatatc
     4501 agcgagcact acgaaacacg ctacaacgaa gtgctgcata tcatggaata tcgcagaagg
     4561 catcccgaca ccgagaaacc ggagcctttc cgcatcttgg atgagatgat ggaaaacagt
     4621 atctggatag aaatcaacga actgggatat gcctgcactg tcaaaacaat cgaaaatctg
     4681 atttactccg atttcagcca gtcgtatcat cccattcgcg agtattttga attactgccc
     4741 gaatgggacg gaaccgacta tatccgtatc ctggcggata gtgtccagac gagtcatcag
     4801 gagttttggg cggaatgcct ggaacgttat ctggtggcga tgtgcgcagc ggctactcaa
     4861 gagaatattg tcaatcacac ggtacttctg ctatgcagcg agatacagaa cataggaaag
     4921 actaccttta tcaataatct cctgcctccc gaactgcgtg cttatctctc taccggacta
     4981 atcaattcga acaataaaga tgatttagcc aaaataacgc aggccatgct gatcaatctc
     5041 gacgagtttg aagggatgag cggtcgg
//
