LOCUS       c00288_gnlPro..         4752 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CILBIEBH_288.
ACCESSION   c00288_gnlPro..
VERSION     c00288_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 11:23:08
            Original ID  :: gnl|Prokka|CILBIEBH_288
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4752
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..861
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: HBT"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg288_1"
                     /sec_met_domain="HBT (E-value: 4.8e-18, bitscore: 53.9,
                     seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="IQNEARLNNLKVIKEEEFSVAIDFAPCKCMLVSDDEEALVSLEGH
                     WKRRLNGALDVFRSEPYFLEVVPCAIDKANTLGALLEELDVKREEVIAIGDGVCDVTMI
                     QLAGLGVAMGHSQDSVKVCADYVTASNEEDGVALAVEKAIIAEVRAAEIPLDQLNAQAR
                     HALMGNLGIQYTYADEDRVEATMPVDHRTRQPFGILHGGATLALGETVAGLGSMILCQP
                     DEIVVGMQVSGNHISSAHEGDTVRAVATIVHKGRSSHVWNVDVFTSTNKLVSSIRVVNS
                     VMKKR"
     protocluster    1..4752
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:4485]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..4485
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /neighbourhood="10000"
                     /product="TPP_fatty_acids"
                     /protocluster_number="1"
     cand_cluster    1..4752
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /kind="single"
                     /product="TPP_fatty_acids"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4752
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_fatty_acids"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and (minimum(2, [ACD1_car,
                     ACP_syn_III_C, AcetylCoA_hyd_C, AcetylCoA_hydro,
                     Acyl-CoA_dh_1, Acyl-CoA_dh_2, Butyrate_kinase,
                     CT_Propionyl_CoA1, CT_Propionyl_CoA2, CT_Propionyl_CoA3,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_2_CAF, CoA_trans_6_Glutaconate, E1_dh, ECH_1,
                     ECH_2, HBT, HBT_2, HCDH, HpaB, Ketoacyl-synt_C, Thiolase_C,
                     Thiolase_N, adh_short, adh_short_C2]) or FA_synthesis) and
                     not (PFL-like or PFL_6_pcresol or PFL_9_tma or Gly_radical
                     or Gly_430_pcre or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and not
                     (POR or POR_N))"
                     /tool="gutsmash"
     CDS             858..1970
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Chorismate_bind"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg288_2"
                     /sec_met_domain="Chorismate_bind (E-value: 1.3e-58,
                     bitscore: 186.9, seeds: 119, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIDEKSNLTTIDALIQRKQPFAVYRVPGEKYPRLLTEDVGAVRLI
                     FDLKELNGQRGFVIAPFRIDKSCPIVLIQSDRTGQPLPMEIVAEEEQDLQSYPEESFHT
                     LCTGKYATCFHTFIEALRDATFDKLVLSRSLTIGKNPEFSPSAVFRAACQRYIHSYIYL
                     CYTPQTGVWLGSTPEIILSGEKNEWNTVALAGTQPLQNGKLPQVWDDKNRQEQDYVASY
                     IRRQLLSLGIRSTESGPYPAYAGALSHLKTDFHFSLKDNKNLGDLLKVLHPTPAVCGLP
                     KEEAYRFILENEGYDRKYYSGFIGWLDPEGRTDLYVNLRCMHIEDEQLTLYAGGGLLAS
                     SELNDEWQETEKKLQTMRRILVSAPIMMNH"
     CDS             1990..3657
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg288_3"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.4e-09, bitscore:
                     25.6, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYSDKKNILQLVALLEAHGITKVVLCPGSRNAPIVHTLSTHPGFT
                     CYAMTDERSAGYFAIGLALNGGHPAAVCCTSGTALLNLHPAVAEAYYQNIPLVVISADR
                     PAAWIGQMAGQTLPQPGVFQTLVKKSVNLPEIQTEEDEWYCNRLVNEALLETNHHGKGP
                     VHINIPISEPLFQFTVESLPEVRVITRYQGLNVYDRDYNDLIERLNRYQKRMIIVGQMN
                     LIYLFEKRHTKLLYKHFVWLTEHIGNQTVPGIPVKNFDAALYAMPEEKTGQMTPELLIT
                     YGGHVVSKRLKKYLRQHPPKEHWHVSADGEVVDLYGSLTTVIEMDPFEFLEKIAPLLDN
                     RVPEYPRVWENYCKTIPEPEFGYSEMSAIGALIKALPESCALHLANSSVIRYAQLYQVP
                     STIEVCCNRGTSGIEGSLSTAVGYAAGSDKLNFIVIGDLSFFYDMNALWNINVRPNLRI
                     LLLNNGGGEIFHTLPGLDMSGTSHKYITAVHKTSAKGWAEERGFLYQRVENEEQLAEAM
                     KTFTQPEAMEQPVLMEVFSNKNKDARILKDYYHQLKQK"
     CDS             3661..4485
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_fatty_acids: ECH_1"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg288_4"
                     /sec_met_domain="ECH_1 (E-value: 8.6e-83, bitscore: 265.8,
                     seeds: 9, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTQREWTTIREYEDILFDYYNGIARITINRERYRNAFTPTTTAE
                     MSDALRICREEADIDVIVITGAGDKAFCSGGDQNVKGRGGYIGKDGVPRLSVLDVQKQI
                     RSIPKPVIAAVNGFAIGGGHVLHVVCDLSIASENAIFGQTGPRVGSFDAGFGASYLARV
                     VGQKKAREIWFLCRKYNAQEALDMGLVNKVVPLEQLEDEYVQWAEEMMLLSPLALRMIK
                     AGLNAELDGQAGIQELAGDATLLYYLTDEAQEGKNAFLEKRKPNFKKYPKFP"
     CDS             4489..4752
                     /locus_tag="ctg288_5"
                     /transl_table=11
                     /translation="MDCKIDIIPRVLHFKQPAGTSRGTYTTRKVWYLHFTAPEFPNWVG
                     IGECAPLPNLSCDDLPDYEEVLAKICRQVENQGGKLDMEALCK"
ORIGIN
        1 atccagaatg aagctcgctt gaataatttg aaagtgatca aggaagagga attttctgta
       61 gcaattgact ttgctccgtg taagtgtatg cttgtcagcg atgacgagga agcgttggtt
      121 agtctggaag gacactggaa gagacggttg aacggagctt tggatgtttt ccgttccgaa
      181 ccttattttc tggaagtagt gccgtgtgcg attgataaag ccaacacatt gggagcttta
      241 ttggaagaac tggatgtgaa gcgtgaagaa gtcatcgcta taggagacgg tgtgtgcgat
      301 gtaacgatga ttcagctggc aggactggga gtggcgatgg ggcattcgca ggattcggta
      361 aaggtctgtg cggattatgt gacagcttcc aatgaggaag acggagtggc tctggctgtc
      421 gaaaaggcga taatagcgga agttcgcgct gcagagattc ctttggacca gttgaatgca
      481 caggcgcgtc acgcattgat gggtaatctg ggtatccaat atacatatgc cgacgaagac
      541 cgtgtagagg cgacgatgcc ggtagatcac cgtacccgtc agccgttcgg tattttgcac
      601 ggtggagcta cgcttgcatt gggagaaaca gttgccggac tcggctcgat gattctctgt
      661 cagccggatg aaattgtggt aggaatgcag gtcagcggaa atcatatatc ttctgcgcac
      721 gaaggtgata cagtacgtgc agtagcgacg attgtacaca aaggacgttc ctcccatgta
      781 tggaatgtgg acgtttttac ttcaaccaat aaactggtgt cctctatacg ggtggtcaac
      841 agtgtgatga aaaaaagatg attgacgaaa aaagtaactt gacaactatt gatgcactta
      901 ttcagcggaa acagcccttt gctgtttatc gtgttcccgg ggagaagtat cctcgtctgt
      961 tgacagaaga tgtcggagct gtccgcttga ttttcgattt gaaagaactg aatggacaaa
     1021 gaggatttgt gattgctccg ttccgtatag ataagtcatg tccgattgtg ttgatacagt
     1081 ccgacaggac tggacaaccg ttgcctatgg aaatcgtggc agaggaagag caggacttgc
     1141 aaagttatcc ggaggaatct tttcataccc tttgtactgg gaagtatgcg acctgttttc
     1201 atacatttat tgaagccttg cgtgatgcta cctttgataa actggtgctt tcccgtagtc
     1261 tcactattgg aaagaatccg gagttttccc cttcagctgt ttttcgtgca gcttgtcaac
     1321 gttatattca ttcctatatc tatctgtgtt atactcctca gacgggcgtg tggctgggaa
     1381 gtacccccga aattatattg tcgggcgaaa aaaatgaatg gaacacagtt gccctggcag
     1441 gaactcaacc tttgcagaat ggtaaacttc cgcaagtatg ggatgacaaa aaccgtcagg
     1501 agcaggatta tgtggcgtcc tatatccgtc ggcagcttct ttcgctgggc attcgctcca
     1561 cagagagcgg accttatccg gcttatgccg gtgccttgtc acatctgaaa actgatttcc
     1621 atttctcttt gaaagataat aaaaatctgg gagaccttct gaaagtcctg catccgactc
     1681 ctgccgtgtg cggactgcct aaagaagaag cctaccggtt cattttggaa aatgaagggt
     1741 acgatcgtaa gtattattcc ggttttatcg gttggctcga tccggaggga cgcacagatt
     1801 tgtatgtgaa tctgcgttgc atgcacattg aagacgagca attgacactg tatgccggtg
     1861 gcggattgtt agcctcttca gagttgaatg atgaatggca ggaaacggaa aagaaattgc
     1921 agaccatgag gcgtattctt gtgtctgctc caataatgat gaatcactaa caagctaatc
     1981 actatcaata tgtattcgga caagaaaaat atactccagt tggtcgcgct gcttgaggcg
     2041 catggaatta cgaaagtagt gttatgtccc ggtagccgta atgcacctat tgtgcatacg
     2101 ctgtctactc atccgggttt tacttgttat gctatgacgg atgaacggag tgccggttat
     2161 tttgcgattg gtcttgcgct gaatggcggg catcctgctg ccgtttgctg tacttccggt
     2221 acagcgttgc tcaatcttca tcctgctgtg gcagaagcgt attatcagaa tataccttta
     2281 gttgttatct ctgccgatcg gcctgctgcc tggattggac agatggcggg acagacattg
     2341 ccgcagcccg gtgtttttca gacattggtg aagaagtcgg taaaccttcc ggagattcag
     2401 acagaagagg atgaatggta ctgtaaccgc ttggtgaatg aagccttgct ggaaaccaac
     2461 catcatggaa aaggtccggt acatattaat attcctatat ccgaaccttt gttccagttt
     2521 acggtggagt cgcttcccga agtacgtgtc attacgcgct atcaggggct gaatgtatac
     2581 gaccgggatt acaatgacct gatcgaacgc ttgaacagat atcagaagcg gatgattatt
     2641 gtcggacaga tgaacttgat ttacttgttt gagaaacggc ataccaaatt gttatataaa
     2701 catttcgtgt ggctgaccga gcatatcggt aatcagacag tacccggtat tccagtgaag
     2761 aattttgatg cggcgcttta tgcgatgccc gaagagaaaa ccggccagat gactcccgag
     2821 ttattgatta cttatggcgg acatgtggtt tccaagcggt tgaaaaaata tcttcggcag
     2881 catccgccta aagaacattg gcatgtgtct gctgatggag aagtggttga cctttacggt
     2941 tctctgacta ccgtcatcga aatggacccg tttgagtttc tggagaaaat agctccattg
     3001 ctggataacc gcgtgccgga gtatccccgt gtatgggaga attattgcaa gaccattccc
     3061 gaaccggagt tcggatattc ggaaatgtcg gctatcggtg ctttgataaa agcattgccg
     3121 gaatcatgtg cattgcatct ggcaaatagt tctgttatcc gctatgctca gttataccag
     3181 gttccttcta cgattgaggt atgctgtaat cggggtacaa gcggcatcga aggctcgttg
     3241 tccacggcag tgggatatgc cgccggttcg gataaactaa attttatagt tataggtgac
     3301 cttagtttct tttatgatat gaatgctttg tggaacatca atgtccgtcc caatctacgc
     3361 attcttttgc tgaataacgg aggaggggag attttccata cattacccgg actggatatg
     3421 tcgggtacat cgcacaagta tattacagcc gttcacaaga catctgccaa aggatgggcg
     3481 gaagaacgcg gattccttta tcaacgggta gagaatgaag aacagcttgc cgaagcgatg
     3541 aaaaccttca cacaaccgga agccatggaa caaccggttc tgatggaagt attcagcaat
     3601 aaaaataaag atgcacgtat actgaaagat tattatcatc aactaaaaca aaaatagatt
     3661 atgtcaacac aaagagaatg gacaaccatc agagaatacg aagatatcct ctttgattac
     3721 tataatggga ttgcccgcat caccattaac cgtgaacgct atcgaaatgc atttactccg
     3781 actacaacgg ctgaaatgag tgacgcattg cgcatttgcc gggaagaagc ggatattgat
     3841 gtgatcgtga ttacgggagc tggtgacaag gctttctgtt cgggcggtga ccagaatgtg
     3901 aaagggcgtg gcggctatat cggcaaggac ggtgttcccc gtctgagtgt tctggatgta
     3961 cagaaacaaa tccgcagcat tccgaaaccg gtaattgctg ccgtaaatgg atttgccatt
     4021 ggtggcggac acgtgcttca tgtagtttgc gatttatcga ttgcttcgga gaatgcgatc
     4081 ttcggacaga ccggtcctcg cgtaggtagc tttgatgcag gtttcggagc ctcttatctg
     4141 gctcgtgtcg tcggtcagaa gaaggcacgt gagatatggt tcctttgccg gaaatacaat
     4201 gcgcaggaag cgttggatat gggattggtg aataaagtag ttccattgga gcaactggag
     4261 gatgagtatg tgcaatgggc ggaagaaatg atgctgctca gtccgttggc cttgcggatg
     4321 attaaagccg gattgaatgc cgaactggac ggccaggcag gtattcagga gctggcaggg
     4381 gatgcgacct tgctttacta tctgacggat gaggctcagg aaggaaagaa tgcatttctg
     4441 gagaaacgta aacctaactt taagaaatat ccgaagttcc cttaaacaat ggattgcaaa
     4501 atagacatta ttccgcgggt gcttcatttc aagcagccgg ccggaacttc acgaggcaca
     4561 tacacaacgc gaaaagtctg gtatcttcat tttactgctc ccgaatttcc caattgggta
     4621 gggatcgggg agtgcgctcc tctgccaaat cttagctgtg atgatcttcc cgattacgag
     4681 gaagtgctgg ctaaaatctg ccggcaggta gagaatcagg gaggtaagct ggacatggaa
     4741 gctctatgca ag
//
