LOCUS       c00318_gnlPro..         4424 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|CILBIEBH_318.
ACCESSION   c00318_gnlPro..
VERSION     c00318_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 11:23:08
            Original ID  :: gnl|Prokka|CILBIEBH_318
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4424
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4424
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:3989](-)"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      complement(3..3989)
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..4424
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4424
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             complement(3..3989)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg318_1"
                     /sec_met_domain="HGD-D (E-value: 6e-30, bitscore: 92.1,
                     seeds: 370, tool: rule-based-clusters)"
                     /sec_met_domain="BcrAD_BadFG (E-value: 2.2e-36, bitscore:
                     112.8, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKIGIDVGSTTAKLVAVDENDNLLFSKYERHNAKAKETILSFLK
                     ELLSEIGDKDISVRITGSIGMGISEKCSLPFVQEVVAAAKAIQKDYSHVTSMIDIGGED
                     AKVVFFNDAEATDLRMNGNCAGGTGAFIDQMAIILGVNIDELNQLAMNATQVYPIASRC
                     GVFCKTDIQNLIAKNVSRENIAASIFRAVAVQTVVTLAHGCDITTPVLFCGGPLTFIPA
                     LRKAFIDYLSLDEKEIILPANGTLLPALGAALFHLDKEDYCKLSHLIDKIDTTLNGNGN
                     LASTGLEPVFASNEEYEAWKERISRHKMISSGLKAGTQDVFIGIDSGSTTTKIVVLDED
                     SRLLYSYYNINGGNPIKAVEEGLNQLNDECLKQNAVLNIKGSCSTGYGEDLIRSAFQLH
                     SGIIETIAHYMAAHYLNKDVSFILDIGGQDMKVIFVNNGVIDRMEINEACSSGCGSFLE
                     TFAKSLGYSAQEFSLAACHSEAPCDLGTRCTVFMNSKVKQVLREGATINDIAAGLAYSV
                     VKNCLYKVLKLKDISVLGKDIVVQGGTMRNDAVVRAIELLSGAEVARCDTPELMGAFGC
                     ALYAMKHQGESVSLDEIINKAQYSARSLYCKSCDNRCLVIRYEFESGKSYYSGNRCEKV
                     FTNGESSNRKGLNVYRQKEELLFHRSAEIAAPEQIIGIPRCLNMYEEYPFWHTLFTSCG
                     IQVCLSDPSNFRKYEHNARMVMSDNICFPAKLVHSHVQDLIEKKVDRIFMPFVIFERKG
                     MEQNSYNCPIVTGYSEVIKSVQSEGIPVDSPAITFKDRNLLFKQCREYLSGLSVDDRTI
                     QQAFQKAESEQHSFINTLVDYNKNILQQTGKGETLTVMLAGRPYHTDSLIQHKVSDMLA
                     DMGVNVIADDLVRQMDIPTDDAHFVAQWAYTNRILKAAKWCATQGKNIQFVEMTSFGCG
                     PDAFLVDEVRDLLMRHNKSLTLLKLDDINNIGSMKLRVRSMIESLKLANADGTEDGVKD
                     FTTVPVYDKSYRDRKILVPYFTPFISPLIPAIMKVAGYDAENLPLSDNDSSEWGLKYAN
                     NEVCYPATLIVGDIIKALKSGKYDISKIAVAITQTGGQCRASNYISLIKKALVDAGYTD
                     IPVISISVGSDIDNDQPAFKVNWMKVVPITFHAVLYSDCIAKFYYASVVREKEAGASAK
                     LRDKYLQLAPEVILRRDIKGLNSLLQSAITEFNEVCRAVDTPKVGIVGEIFLKFNPFAQ
                     KEIIDWLIGRKIEVVPPILADFFMQGFVNRKVNTDSYIKNRQLPEFIYDWIYKFVKKQI
                     DKIQIQLLRRKDGRLLGHLPVIAVRLMVSLDLLGIIGRKQQENKD"
     CDS             complement(4107..4424)
                     /locus_tag="ctg318_2"
                     /transl_table=11
                     /translation="QCEANYSNAHGTPWVYKHQNIGKLVGMPVPGTMTSVSWETLQDPS
                     LVFGIPIIGYRLPDGSYLENTQLEPDIKVANSPETIVKGEDTQLKMAVDELLKEIDSRK
                     K"
ORIGIN
        1 ggatctttat tttcctgctg tttacggcct atgataccca gaagatcaag gcttaccatc
       61 aggcgtactg ctatgacggg cagatggccc agaaggcgtc catctttgcg gcgcagcagt
      121 tgtatctgga ttttatcaat ctgtttcttt acaaacttgt agatccagtc gtaaataaac
      181 tcaggcaact gtctgttttt tatgtaagaa tccgtattga ctttcctgtt cacaaacccc
      241 tgcatgaaaa agtcagccag aatcggcgga accacttcta tcttgcggcc tatcagccaa
      301 tcaatgatct ctttttgtgc gaaaggattg aatttcaaga agatttctcc tacaattccg
      361 actttaggag tatctacagc cctacatacc tcattaaatt cagtgattgc cgattgcaac
      421 aaagagttca acccctttat gtctcttctc agtatcactt caggtgccag ttgcaggtat
      481 ttatcacgca gctttgccga tgcacctgct tctttctcac gtaccacaga cgcataataa
      541 aacttagcaa tgcaatcact atacaaaacg gcatgaaaag ttatcggtac tactttcatc
      601 cagttgacct tgaacgcggg ctggtcatta tctatgtcac tgcccacaga gatagagatt
      661 accggaatat ccgtatatcc ggcatccacc aacgcctttt ttatcaagga aatatagttg
      721 ctggcacggc attgtcctcc cgtttgggtg atggctacgg ctatttttga aatatcatat
      781 ttcccgcttt taagtgcttt tataatatcg ccgactatca gagtagccgg ataacagacc
      841 tcattattcg catacttcaa cccccattca cttgaatcat tatcactgag aggaaggttt
      901 tccgcatcat aaccggctac tttcataata gcaggaatca aaggtgaaat aaacggagta
      961 aagtaaggaa cgagtatctt acggtcacgg taagacttgt cataaacagg aacggtcgta
     1021 aagtccttta caccgtcttc cgttccatcc gcattcgcaa gtttgaggct ttcaatcatg
     1081 gaacgtactc tcaacttcat tgaaccgata ttattgatat catccagttt caataaggtc
     1141 agcgatttgt tatgacgcat cagcaaatcc cttacttcat ccaccagaaa agcatccggt
     1201 ccacatccga acgaagtcat ctctacaaac tgaatattct ttccttgtgt cgcacaccat
     1261 ttcgcagcct tcaggatacg gttcgtatac gcccattgag caacaaaatg cgcatcatcc
     1321 gtcggtatat ccatctgacg caccaaatca tccgctatca cattcactcc catatccgca
     1381 agcatatccg agactttgtg ctgtatcaga gaatcggtat gataaggacg tccggcaagc
     1441 atgacagtca aagtctctcc ctttccagtc tgctgcaata tgtttttgtt ataatcgacc
     1501 aatgtattaa taaaagagtg ctgttcgctt tccgcttttt ggaatgcttg ctgaatcgtc
     1561 cgatcgtcca cgctcaaacc gctcagatat tcacggcatt gtttaaacaa cagattccgg
     1621 tctttgaaag ttattgcagg ggaatcaacc ggtatacctt cggactgcac acttttgata
     1681 acctccgaat aacctgtcac aatcggacaa ttataactgt tttgttccat cccttttctt
     1741 tcgaaaataa caaaaggcat aaaaatccgg tcgaccttct tttctatcaa gtcctgaaca
     1801 tgactatgta ccagcttagc cggaaagcag atattgtccg acatcaccat acgcgcattg
     1861 tgctcatact ttctgaaatt ggacggatca gacaaacaga cttgtatgcc acaggaagtg
     1921 aaaagagtgt gccagaatgg atattcctcg tacatattca gacatcgggg gattccgata
     1981 atctgctcgg gtgctgcaat ctctgcgctg cgatgaaaca aaagctcttc tttttgccga
     2041 tagacattca gacctttccg gttactgctc tctccattcg tgaaaacttt ctcgcacctg
     2101 tttcccgaat aataactttt gccgctttcg aactcgtaac gaatgaccag acaacgatta
     2161 tcgcaactct tgcaatataa agaacgtgcc gaatattgag ctttattgat aatctcatcc
     2221 aatgatacgg actccccctg atgtttcatc gcatataatg cacatccgaa tgctcccatc
     2281 aattcgggtg tatcacacct tgccacttcc gctcccgaga gaagttcaat ggcacggacc
     2341 accgcatcat tgcgcatggt gcctccctgc acaacaatat cttttcccaa aacagatata
     2401 tctttcagtt tcaacacctt atacaggcaa ttcttgacaa cggaatatgc cagtccggcg
     2461 gcaatatcat tgatcgttgc cccctccctg agtacctgtt tcacttttga gttcataaaa
     2521 acggtgcacc ttgttcccaa atcacacggt gcttccgaat ggcaggctgc cagagaaaac
     2581 tcttgtgccg aataccccag agatttagca aacgtttcaa gaaaagaacc acatccggaa
     2641 gagcatgctt cattaatctc catccggtca atcacgccat tattcacaaa aataaccttc
     2701 atatcctgtc cgccgatatc gaggataaaa gacacatcct tgttcagata atgcgctgcc
     2761 atataatggg cgatcgtttc gataatcccc gaatgaagct gaaaggcaga cctgataagg
     2821 tcttccccat atccggtaga acagcttcct tttatattga gtacggcatt ctgcttcaaa
     2881 cattcatcgt tcaactgatt cagcccctct tccacagctt ttatcggatt gccgccatta
     2941 atattataat aagaatacag aagccttgaa tcttcatcca gtacaacaat ctttgtcgta
     3001 gtagaacctg aatcgatgcc gataaagaca tcctgtgttc cggctttcaa gccggaagat
     3061 atcatcttat gacgggatat tctttctttc catgcttcat attcttcatt acttgcaaaa
     3121 acaggttcca atcctgttga ggcaaggtta ccgttaccgt ttaaagtagt gtcaatctta
     3181 tcgatcaaat gggacagttt acagtaatcc tctttatcaa gatgaaatag agcagcacct
     3241 agtgcaggga gcaaagtgcc gttggccggc agtatgatct ctttttcatc gagggacaaa
     3301 taatcaatga aggctttccg cagtgccgga atgaaggtta acggaccacc acaaaacaat
     3361 acgggagttg taatatcgca accgtgcgcc agcgtcacta ccgtttgaac tgctaccgca
     3421 cgaaagatgg aagccgcaat attctcacga cttacatttt tagctatcag attctgaata
     3481 tccgttttac agaaaacgcc acaacgggag gcaataggat atacctgtgt cgcattcatg
     3541 gctagttgat tcaattcatc aatattcact cccagtataa tcgccatctg gtcaataaaa
     3601 gcacccgtcc ctcctgcgca attgccgttc atacgcaaat cggtcgcttc ggcatcatta
     3661 aagaagacga ctttcgcatc ttctcctccg atgtctatca tggaagtcac atgagaataa
     3721 tccttttgta tcgctttcgc tgccgcaaca acctcctgta caaacggcaa cgaacatttc
     3781 tctgaaattc ccattccgat agagcctgtt atcctcacgg aaatatcttt atctccgatt
     3841 tcagataaca gctctttcaa gaatgataga attgtctctt ttgctttcgc attgtgtctc
     3901 tcgtactttg aaaataatag attatcattt tcatcgacgg caactaactt cgccgtcgtt
     3961 gaacctacat caattcctat tttaatcatg ttctacacta tgttccccta gtttgacact
     4021 attgtcaaac acaaaagtaa gaataagttg gacacatcat ttcaataagc ttttttttcg
     4081 gttcttttaa cacaaacgaa cgatgtctat ttcttccgac tatctatttc tttaagcaat
     4141 tcatccacag ccattttaag ttgcgtatcc tctcctttca ctatagtctc cgggctgttg
     4201 gcaaccttga tgtcaggttc caactgagta ttttccagat aactgccatc cggtagacga
     4261 taaccgatga taggaatccc aaataccaat gaagggtcct gcaaagtttc ccaagataca
     4321 cttgtcatag ttcccggaac aggcatacct accagttttc caatgttctg atgcttgtac
     4381 acccacgggg tgccatgcgc attcgaataa ttggcttcac actg
//
