LOCUS       c00186_gnlPro..         7547 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KKMBOKBE_186.
ACCESSION   c00186_gnlPro..
VERSION     c00186_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 15:14:02
            Original ID  :: gnl|Prokka|KKMBOKBE_186
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7547
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7547
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3256:6770]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3257..6770
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..7547
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7547
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             2..355
                     /locus_tag="ctg186_1"
                     /transl_table=11
                     /translation="KSLGLSDIRILLNTLGDEYFILQNYKKVKTVEELAQLGGYTVSTL
                     RRIFNNVFHEPVYEWMQARRKEGILDDLNNSEYSISEICYKYGFESLPHFSNFCKKFFG
                     ASPRMLRTSKKNV"
     CDS             703..2229
                     /locus_tag="ctg186_2"
                     /transl_table=11
                     /translation="MRTKRAFKEFCVLGGLASSVCGVAQERPNIIVFLVDDMGLMDTSV
                     PFIADESGQPVRHPLNDWYHTPNMERLAKQGICFSTFYAQSVSSPSRASIMTGQNATRH
                     GVTNWINAESNNRNPFGPPQWNWKGLRKDMPTMPRVLQQAGYKTIHVGKAHFGCMGSEG
                     ENPLNIGFDVNIAGSGIGHPGSYYGEWGYGHIKGQKIRAVPDLEKYHGTDTFLSEALTI
                     EANREITKAVEEKRPFYLNMAHYAVHSPFQADKRFLSRYTDPDKNEQARAFATLIEGMD
                     KSLGDIMDQLEKLEIAENTLILFLGDNGGDAPLGDERGYGSSAPLRGKKGTEFEGGMRV
                     PFIAAWAKPEKKSKVQKNLPIEVGSMQTQLGTIMDIYPTVLSVAGCEVPQNYVIDGFDL
                     KKQLSGKVDKKRPESFLMHFPHAHRGSYFTTYRMGDWKLIYYYLPETPKQPKALLYNLK
                     DDPEERNELSSAHPDKCREMIQEMSAQLEKEGALYPVDKQGNELKPFVYF"
     CDS             complement(2407..2631)
                     /locus_tag="ctg186_3"
                     /transl_table=11
                     /translation="MTEIMLDKQETLNEIGTKIRKLRESKNLSIQEFADKLEMEYNNVI
                     RIEKGRTNFTIGTLVKIANSLEVNLKDIV"
     CDS             2757..3149
                     /locus_tag="ctg186_4"
                     /transl_table=11
                     /translation="MDTLNLHSNFDNLLYIGRSVLNNTSSHIQKLFFKKEMCIYEYLYK
                     EEVSKGIEIKIDDVVLVCALEHDKCNKSILYFNDVANIARYIHYCNSNFEYKEELNGWI
                     MSDSYLALSISEDDYEKRFAFIQTLL"
     CDS             complement(3257..4012)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer4_7"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_5"
                     /sec_met_domain="Fer4_7 (E-value: 5.9e-13, bitscore: 38.5,
                     seeds: 208, tool: rule-based-clusters)"
                     /sec_met_domain="Fer2_3 (E-value: 4.2e-26, bitscore: 80.3,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 2.6e-07, bitscore: 20.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKNISFTLKVWRQAGPKAKGAFETYQMKDIPGDTSFLEMLDILN
                     EQLISERKEPVVFDHDCREGICGMCSLYINGHPHGPATGATTCQIYMRRFNDGDTITVE
                     PWRSAGFPVIKDLMVDRTAYDKIMQAGGYVSVRTGAPQDANAILIPKPIADEAMDAASC
                     IGCGACVAACKNGSAMLFVSAKVSQLNLLPQGKPEALRRAKAMLSKMDELGFGNCTNTR
                     ACEAECPKNISISNIARLNRDFIIAKLKD"
     CDS             complement(4049..6028)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 200.6; E-value:
                     1.5e-61)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_6"
                     /sec_met_domain="FAD_binding_2 (E-value: 2e-62, bitscore:
                     200.6, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 4.3e-37,
                     bitscore: 116.1, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKIDSKIPEGPVAEKWTNYKAHQKLVNPANKRRLDIIVVGTGLA
                     GASAAASLGEMGFRVFNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTVKG
                     GDYRAREANVYRLAEVSNAIIDQCVAQGVPFAREYGGTLDNRSFGGAQVSRTFYAKGQT
                     GQQLLLGAYSALSRQVNVGTVKLFTRYEMQDVVIIDGRARGIIAKNLITGELERFAAHA
                     VVIATGGYGNAYFLSTNAMGCNCTAAISCYRKGAVFANPAYVQIHPTCIPVHGDKQSKL
                     TLMSESLRNDGRIWVPKKKEDAIKLQKGEIKGSDIPEEDRDYYLERRYPAFGNLVPRDV
                     ASRAAKERCDAGFGVNNTGLAVFLDFSEAINRLGIDVVLQRYGNLFDMYEEITDVNPGE
                     LAKEISGVKYYNPMMIYPAIHYTMGGIWVDYELQTTIKGLFAIGECNFSDHGANRLGAS
                     ALMQGLADGYFVLPYTIQNYLADQITVPRFSTDLPEFAEAEKAVQAKIDKFMSIQGKES
                     VDSIHKKLGHIMWEYVGMGRTAEGLKKGIAELKAVRKEFETNLFIPGSKEGMNVELDKA
                     IRLYDFITMGELVAYDALNRNESCGGHFREEYQTEEGEAKRDDENFFYVACWEYQGDDE
                     KAPVLIKEPLVYEAIKVQTRNYKS"
     CDS             complement(6066..6770)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_7"
                     /sec_met_domain="SucD_FumR (E-value: 1e-131, bitscore:
                     425.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWLSNSSVGRKVVMSVTGIALVLFLTFHMAMNLVAIISADGYNMI
                     CEFLGANWYALVATAALAALFIIHIIYAFWLTMQNRTARGSERYAVVDKPKTVEWASQN
                     MLVLGIIVIVGLGLHLFNFWAKMQLPELMHNLDMHADTLTLAYAANGAYHIQQTFSCPV
                     YVVLYLIWLFALWFHLTHGFWSSMQSLGWNNKVWINRWKCISNIYSTIVVLGFALVVVV
                     FFVKSLLCGGAC"
     CDS             complement(6891..7406)
                     /locus_tag="ctg186_8"
                     /transl_table=11
                     /translation="MVMNPRLFYLINDVAAYLDESTPACGKYIDLKCQEMVYMITNYYP
                     LPQLRAFFYPISTYTESFQYFVMQNYSKAKNVEEFAHLGGYNTTTFRRLFRNLYGVPVY
                     EWMLEKKREGILEDLQHSNMRITEICNRYGFDSLSHLAHFCKDSFGDTPRALRKKAANG
                     EKIGKITD"
ORIGIN
        1 gaaaagcctg ggtctgtccg atatcaggat tctgctgaac accctgggag acgaatactt
       61 catccttcag aactataaga aagtaaaaac ggtagaagaa ctggctcaac tgggaggtta
      121 taccgtcagc accttgcggc gtatcttcaa taatgtattc cacgaaccgg tatacgaatg
      181 gatgcaggcg cgacgtaagg aaggtatttt ggatgacttg aataactctg agtacagtat
      241 ctccgaaata tgctataaat acggttttga gtctctgcct cacttctcca atttctgtaa
      301 gaagttcttt ggagcttctc cacgtatgct tagaacaagc aagaagaacg tctgataatt
      361 tttcagagag aaagtcaaaa actatttttt tctttgtggg tgtagtacat ttgttgtata
      421 ttgctagctg tttcctttaa aaccagtcta atacatcttt attataaagt cagatcactt
      481 caaataaagc catgataagg cggatacgga aattaaactt tttatttcaa cgaattaaaa
      541 tcaggtttca aagcagaata ataacatgat agattatgct gaatgaatga aagataatag
      601 gatatttttc taatactctt ttgtgtaatc agaagagagt ctgagtttga aatctattat
      661 ggcacgtaca ataactagga tataaatcat aaatactatg taatgagaac aaaacgagct
      721 tttaaggaat tttgtgtatt gggtggtttg gcatcttccg tttgcggagt agctcaggag
      781 cgcccgaata ttattgtttt ccttgtagat gacatgggac ttatggatac gtcggttcct
      841 tttatcgcag atgagagtgg tcagccggtg cgtcatccgc tgaatgactg gtatcatacc
      901 ccgaatatgg agagactggc taaacagggt atttgttttt ctacttttta cgcacagagc
      961 gtgagttccc cttccagagc ttccatcatg accggacaga atgcgaccag gcatggagtc
     1021 actaactgga ttaatgctga gagcaacaat cggaatcctt tcggaccgcc ccaatggaac
     1081 tggaaaggtc tcagaaaaga tatgcctacc atgccgagag tcctgcaaca agccggatat
     1141 aaaaccattc atgtaggaaa agcacatttc ggctgtatgg gaagcgaagg agagaatccg
     1201 ctgaatatcg gctttgatgt aaatatcgca ggttccggca ttggacatcc cggcagctat
     1261 tatggcgaat ggggatacgg gcatatcaag ggacagaaga ttagagcagt tcctgacctg
     1321 gagaaatatc acggtacaga taccttcctg agtgaagcgc tgactataga agcgaatcgg
     1381 gagatcacca aagccgtaga agaaaaacga cctttctatc tgaatatggc ccattacgca
     1441 gttcattcac ctttccaggc agacaaacgt ttcttgtcac gctacaccga cccggataag
     1501 aacgagcagg caagagcttt tgctacattg atagaaggca tggataaatc attaggcgac
     1561 atcatggacc aactcgaaaa gctggaaatt gccgaaaata cgctgattct ctttttagga
     1621 gataacggtg gtgatgctcc attgggagat gaacgtggct atggttcttc cgctccgctg
     1681 agaggtaaaa aggggacgga gtttgaaggt ggtatgcgtg ttcctttcat cgcagcttgg
     1741 gcaaagcctg agaagaagag caaagtacag aagaacttgc ctattgaagt aggaagtatg
     1801 cagacacagt tgggcacaat catggatatc tatccgactg ttctgtctgt ggcaggatgc
     1861 gaagttcccc aaaactacgt gatagacgga ttcgatctga aaaagcaact gagcggtaag
     1921 gtggacaaga agcgtcccga gtctttcctg atgcacttcc ctcacgcaca ccgcggcagc
     1981 tacttcacca cctatcgcat gggcgactgg aaactgattt attattatct tcccgagact
     2041 ccgaaacaac ccaaagcctt gctttacaat ctgaaagacg atccggaaga aagaaatgaa
     2101 ctttcttccg cacatccgga taaatgcagg gagatgattc aggaaatgtc tgctcaatta
     2161 gagaaggaag gagctcttta tccggtggat aaacaaggaa atgagttgaa accttttgtt
     2221 tacttttgat tggcattgat tttggtttgc aagagtattg atgtcttatc cgcaaattgt
     2281 tttaaatgta attgcataga catttcagtc aatatatgaa gttcagttga taatagttat
     2341 gcttcatata tacagctcct taggttagcg ttctaaggag ttgtataatc cttgcatctc
     2401 aaattgctat actatatcct ttaaatttac ttctagggaa ttggctattt tcacaagagt
     2461 accaatagtg aaattagttc tccctttctc tattcgaata acgttgttgt actccatttc
     2521 aagtttatct gcgaattctt ggatagatag attcttactt tcccgtaatt ttctaatttt
     2581 agtacctatc tcatttaaag tttcctgttt atcaagcata atctctgtca tatatgattg
     2641 ttttcaaagg aaagtattta tatttgcata tccgcaatca tatatgattg tatggtgaga
     2701 tataataatg ctaatggaat attcagttga gtaataacaa taaaaaaaat gatgagatgg
     2761 atacacttaa tttgcattca aactttgaca acctactata tataggtaga agcgtattaa
     2821 ataatacgag cagtcacatt caaaaattat tctttaaaaa agagatgtgt atatatgagt
     2881 acttgtataa ggaggaggta agtaaaggca ttgaaattaa aatagatgat gttgtcttag
     2941 tctgtgcttt agaacatgat aaatgcaata agtccattct ttattttaat gacgtagcaa
     3001 atatcgcacg ttacattcat tactgtaaca gtaattttga atataaagaa gaactcaatg
     3061 ggtggatcat gtcagatagt tatcttgctt tatctatatc tgaggatgat tatgagaagc
     3121 gatttgcgtt tatacaaact ttactatgaa cttaactgag tcgttttatt atacttttgc
     3181 taatgagtaa aaagaaaatc ccccgccagc taacgccgac gggggattta tgctattaat
     3241 caaatcaagc tcacgcttag tctttcagtt tagcgatgat gaagtcgcgg ttcaggcggg
     3301 cgatgttgct gatagagatg ttcttcggac attcagcctc gcaagcgcgg gtgttggtac
     3361 agttaccgaa tccgagttca tccatcttag acaacatagc tttcgcacgg cgcagagctt
     3421 ccggtttacc ttgcggaagc aagttcagct ggctgacctt tgcagaaacg aacaacatag
     3481 cagaaccatt cttacaagca gctacacaag caccgcaacc gatacaagaa gcggcatcca
     3541 tagcttcgtc agcgatgggc ttcgggatca ggatagcgtt ggcatcctgc ggagcgcctg
     3601 tacgaacgct tacataacca ccggcctgca tgatcttgtc ataagcagta cggtctacca
     3661 tcaagtcctt gatgacaggg aaaccggccg aacgccaagg ctcaacagtg atggtgtcac
     3721 catcgttgaa acgacgcatg taaatctggc aagtagtagc acctgttgca ggaccgtgcg
     3781 gatgtccgtt gatgtaaaga gaacacatac cgcagatacc ttcgcggcag tcgtggtcga
     3841 atacaaccgg ttcttttctt tcgctgatca gctgttcgtt cagaatatcc agcatttcga
     3901 ggaaggaagt atcaccgggg atatctttca tttggtaggt ttcaaaagcg cctttagcct
     3961 tcgggcctgc ttgacgccat accttcagtg taaatgatat atttttatcc attttcgtct
     4021 aattctttta aatgtttaac ttcaccgatt agctcttgta gttacgggtc tgaaccttga
     4081 tagcttcgta taccagcggt tctttgatca atacaggagc cttttcatcg tcaccctgat
     4141 attcccagca agctacgtag aagaagtttt cgtcatcacg ttttgcttct ccttcttcag
     4201 tctggtattc ttcacggaag tgaccaccac aactttcgtt acggttcaat gcgtcgtaag
     4261 caacgagctc gcccatagtg atgaagtcat acagacggat agccttgtcg agctcaacgt
     4321 tcataccttc cttagaaccg gggataaaca gattggtttc gaattccttg cggactgctt
     4381 tcagttcggc gatacctttc ttcaggcctt ctgccgtacg tcccatacct acgtattccc
     4441 acatgatgtg acccagtttc ttgtggatag aatcaactga ttctttaccc tggatgctca
     4501 tgaacttgtc gatcttagcc tgaactgcct tttctgcttc ggcaaattcc ggaaggtcag
     4561 tagagaaacg aggaacagta atctgatctg ccagatagtt ctggatagtg taaggcaata
     4621 cgaagtaacc gtcggccaga ccttgcatca atgcggaagc accgaggcgg tttgcaccgt
     4681 ggtcggagaa gttacattca ccgatagcga acagaccctt gatggtagtc tgcagttcgt
     4741 agtctaccca gataccaccc attgtgtagt ggatagccgg ataaatcatc atcggattat
     4801 aatatttcac accactgatt tcttttgcca gttcgcccgg attaacgtca gtgatttctt
     4861 catacatatc gaagaggtta ccataacgct gaagcactac atcgatgccc aggcggttga
     4921 tagcttcaga gaaatcgagg aatacggcca gacctgtgtt gttcacaccg aaacctgcgt
     4981 cgcaacgttc tttagctgca cggctggcaa cgtcacgtgg aaccaggtta ccgaatgccg
     5041 gataacggcg ttccagataa tagtcgcggt cttcttcagg aatatcgctt cccttgattt
     5101 cgcctttctg caatttgata gcgtcttctt tcttcttcgg aacccagata cgaccgtcgt
     5161 tacgcagaga ttcggacatc agagtcagtt tggactgctt gtcaccgtgt accgggatac
     5221 aagtcgggtg aatctgaacg tatgcggggt tagcgaatac agcacccttg cggtagcaag
     5281 agatagcagc tgtacagtta caacccatag cgttggtaga caggaagtaa gcgttaccgt
     5341 aaccaccggt agcgattact acggcgtgag cagcgaaacg ttccagttcg ccggtgatta
     5401 agttcttggc gatgataccg cgtgcacgtc cgtcgatgat cacaacgtcc tgcatttcat
     5461 aacgggtgaa caacttcacc gtacctacgt ttacctgacg gctcagtgcg gagtaagcac
     5521 ccagcaagag ttgctgaccg gtctggcctt tagcgtagaa tgtacgtgat acctgtgcgc
     5581 caccgaaaga acggttgtcc agtgtaccgc cgtattcgcg tgcgaaagga acaccttgtg
     5641 ccacgcattg gtcgatgata gcgttggaca cttcggccag acggtaaacg ttggcttcac
     5701 gtgcacgata gtcaccacct tttactgtat cgtaaaacag acggtaaacg gagtcaccat
     5761 cattctgata attctttgca gcattgatac caccctgtgc agcgatagag tgtgcacggc
     5821 gcggagagtc ctgaatacag aagttgaata ctctgaaacc catttcaccg agggatgcag
     5881 cagcagaagc acctgccaga cccgtaccca caacgatgat atccaaacgg cgtttattag
     5941 cggggttcac caatttctgg tgagctttat agttggtcca tttctcagcc accgggcctt
     6001 ctggaatttt tgaatctatc ttgatcataa tattatttac aatttaatca tttacaattt
     6061 actatttagc aagcaccgcc acaaaggagt gatttcacga agaataccac tactacgagt
     6121 gcgaaaccga gtactacaat cgtagaatag atgttagaaa tacatttcca acgattgatc
     6181 cataccttgt tgttccatcc cagtgattgc atggagctcc agaaaccgtg agtcaggtgg
     6241 aaccacagag caaacagcca aatgaggtag agcactacat aaaccggaca agagaatgtc
     6301 tgctggatgt gatatgcacc gtttgccgca taagccagcg tcagtgtatc agcgtgcatg
     6361 tccaggttgt gcatcagttc cggcaactgc atttttgccc agaagttgaa gaggtgcaaa
     6421 ccgaggccta cgataacgat aatgcctaac accaacatgt tttgagaagc ccactctaca
     6481 gttttcggtt tgtcgactac tgcatagcgt tcgctaccgc gcgctgtgcg attctgcatt
     6541 gtcagccaga acgcgtagat gatgtgaata atgaaaagag cagccagtgc tgcggtagct
     6601 accagcgcat accagtttgc tcccaaaaac tcacaaatca tgttgtaacc atcagccgag
     6661 atgattgcaa ctaagttcat cgccatgtga aatgttagaa acaggacaag ggcgataccg
     6721 gtaacgctca tcaccacttt ccttcctaca gatgaattac ttaaccacat aaatgattga
     6781 atttaaatta tttaattgtt agaactaaaa tttcgacgtt ttacacctct tacagactgc
     6841 aaaaataggg gaaatagatt gattgaccaa agaattaaag aaataattcc ttaatcggtg
     6901 atctttccga ttttttcgcc attggccgct ttcttgcgca aggcgcgcgg ggtgtctccg
     6961 aaggagtctt tgcagaagtg tgcaaggtgg gacagcgagt cgaaaccata ccggttgcaa
     7021 atctctgtaa tccgcatatt agagtgctgt aggtcttcga ggatgccttc ccgttttttc
     7081 tccagcatcc attcgtagac gggcacgccg tataagtttc ggaagagacg gcggaaggtg
     7141 gtggtgttat agcctcccag atgtgcgaat tcttcgacgt tcttcgcttt gctgtaattc
     7201 tgcatgacaa agtattggaa actttccgta taggtgctga tcgggtagaa gaaagccctc
     7261 aactggggca ggggatagta attggtgatc atatacacca tctcctgaca tttcaggtcg
     7321 atatatttgc cgcaggcggg tgtcgattcg tcaaggtatg cggctacgtc gttgatgaga
     7381 tagaacaggc gcgggttcat caccattggg gtgtaggtca gcgggccttc tgcctggtcg
     7441 atgatttcac ggtagcggtt ttcgcatagc aacggcagtt cgttgaacca gacccctgat
     7501 tattccggtc cgcctgacgc agggtgccct cacaggtcat gccgcat
//
