LOCUS       c00281_gnlPro..         5459 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KKMBOKBE_281.
ACCESSION   c00281_gnlPro..
VERSION     c00281_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 15:14:02
            Original ID  :: gnl|Prokka|KKMBOKBE_281
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5459
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5459
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:3836](+)"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..3836
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /neighbourhood="10000"
                     /product="Others_HGD_unassigned"
                     /protocluster_number="1"
     cand_cluster    1..5459
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D,
                     HleuD1, HleuD2, HleuS1, HleuS2, Lactoyl-CoA1,
                     Lactoyl-CoA2]) and minimum(1, [ACP_syn_III, ACP_syn_III_C,
                     AcetylCoA_hyd_C, AcetylCoA_hydro, Acyl-CoA_dh_1,
                     Acyl-CoA_dh_2, AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG,
                     Biotin_lipoyl, Carboxyl_trans, CoA_trans,
                     CoA_trans_230_acto, CoA_trans_6_Glutaconate, CoA_transf_3,
                     ECH_1, ECH_2, ETF, ETF_alpha, FGGY_C, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, Mal_decarbox_Al, Peptidase_M22,
                     Peptidase_M23, Thiolase_C, Thiolase_N, ketoacyl-synt]) and
                     not (minimum(2, [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /kind="single"
                     /product="Others_HGD_unassigned"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5459
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Others_HGD_unassigned"
                     /region_number="1"
                     /rules="(minimum(1, [HAAA1, HG2B1, HG2B2, HGD-D, HleuD1,
                     HleuD2, HleuS1, HleuS2, Lactoyl-CoA1, Lactoyl-CoA2]) and
                     minimum(1, [ACP_syn_III, ACP_syn_III_C, AcetylCoA_hyd_C,
                     AcetylCoA_hydro, Acyl-CoA_dh_1, Acyl-CoA_dh_2,
                     AdoHcyase_NAD, AlaDh_PNT_C, BcrAD_BadFG, Biotin_lipoyl,
                     Carboxyl_trans, CoA_trans, CoA_trans_230_acto,
                     CoA_trans_6_Glutaconate, CoA_transf_3, ECH_1, ECH_2, ETF,
                     ETF_alpha, FGGY_C, HCDH, HCDH_N, Hacid_dh, Hacid_dh_C,
                     Mal_decarbox_Al, Peptidase_M22, Peptidase_M23, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and not (minimum(2,
                     [AcetylCoA_hyd_C, BcrAD_BadFG, CoA_trans,
                     CoA_trans_230_acto, Mal_decarbox_Al]) and Lactoyl-CoA2 and
                     Lactoyl-CoA1) and not (minimum(4, [ACP_syn_III,
                     ACP_syn_III_C, Acyl-CoA_dh_1, Acyl-CoA_dh_2, AlaDh_PNT_C,
                     BcrAD_BadFG, ECH_1, ECH_2, ETF, ETF_alpha, HCDH, HCDH_N,
                     Hacid_dh, Hacid_dh_C, IlvN, NAD_Gly3P_dh_N, Thiolase_C,
                     Thiolase_N, ketoacyl-synt]) and HAAA1 and not HleuS1))"
                     /tool="gutsmash"
     CDS             3..3836
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: BcrAD_BadFG"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Others_HGD_unassigned: HGD-D"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg281_1"
                     /sec_met_domain="HGD-D (E-value: 1.1e-29, bitscore: 92.3,
                     seeds: 370, tool: rule-based-clusters)"
                     /sec_met_domain="BcrAD_BadFG (E-value: 4.2e-36, bitscore:
                     112.8, seeds: 18, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="IKAGVFDFAFEIDIHLKALAMNATQVYPIASRCGVFCKTDIQNLI
                     AKNVSRENIAASIFRAVAVQTVVTLAHGCDITTPVLFCGGPLTFIPALRKAFIDYLSLD
                     EKEIILPANGTLLPALGAALFHLDKEDYCKLSHLIDKIDTTLNGNGNLASTGLEPVFAS
                     NEEYEAWKERISRHKMISSGLKAGTQDVFIGIDSGSTTTKIVVLDEDSRLLYSYYNING
                     GNPIKAVEEGLNQLNDECLKQNAVLNIKGSCSTGYGEDLIRSAFQLHSGIIETIAHYMA
                     AHYLNKDVSFILDIGGQDMKVIFVNNGVIDRMEINEACSSGCGSFLETFAKSLGYSAQE
                     FSLAACHSEAPCDLGTRCTVFMNSKVKQVLREGATINDIAAGLAYSVVKNCLYKVLKLK
                     DISVLGKDIVVQGGTMRNDAVVRAIELLSGAEVARCDTPELMGAFGCALYAMKHQGESV
                     SLDEIINKAQYSARSLYCKSCDNRCLVIRYEFESGKSYYSGNRCEKVFTNGESSNRKGL
                     NVYRQKEELLFHRSAEIAAPEQIIGIPRCLNMYEEYPFWHTLFTSCGIQVCLSDPSNFR
                     KYEHNARMVMSDNICFPAKLVHSHVQDLIEKKVDRIFMPFVIFERKGMEQNSYNCPIVT
                     GYSEVIKSVQSEGIPVDSPAITFKDRNLLFKQCREYLSGLSVDDRTIQQAFQKAESEQH
                     SFINTLVDYNKNILQQTGKGETLTVMLAGRPYHTDSLIQHKVSDMLADMGVNVIADDLV
                     RQMDIPTDDAHFVAQWAYTNRILKAAKWCATQGKNIQFVEMTSFGCGPDAFLVDEVRDL
                     LMRHNKSLTLLKLDDINNIGSMKLRVRSMIESLKLANADGTEDGVKDFTTVPVYDKSYR
                     DRKILVPYFTPFISPLIPAIMKVAGYDAENLPLSDNDSSEWGLKYANNEVCYPATLIVG
                     DIIKALKSGKYDISKIAVAITQTGGQCRASNYISLIKKALVDAGYTDIPVISISVGSDI
                     DNDQPAFKVNWMKVVPITFHAVLYSDCIAKFYYASVVREKEAGASAKLRDKYLQLAPEV
                     ILRRDIKGLNSLLQSAITEFNEVCRAVDTPKVGIVGEIFLKFNPFAQKEIIDWLIGRKI
                     EVVPPILADFFMQGFVNRKVNTDSYIKNRQLPEFIYDWIYKFVKKQIDKINETASKFRY
                     FAPFNDIFEEAEGAKSVISLNAQFGEGWLLPAEIVSYARQGVNNVVSLQPFGCIANHIV
                     SRGVEKRIKSFYPDINLLSLDFDSGVSDVNITNRMLLFIDNLKNATMNYDNN"
     CDS             3823..4446
                     /locus_tag="ctg281_2"
                     /transl_table=11
                     /translation="MTTTNNNEDLEKKIIEAAKELFIENGFAETSMSDIAAKVGINRPA
                     LHYYFRTKDKMFQAVFGNIILSFIPKIHDIIIQQDKPVSESIGEIVDAYYKVFTENPCL
                     PLFMVREMHRDMNHLSTTIKELRLEQYLHKIMDVLQEEMNDGRLKTVPLPFVFYTFYSA
                     LTVPFLTKNLSASFFLEKDDSFEDILVQWKPYIVRQMETLLVAS"
     CDS             complement(4454..5125)
                     /locus_tag="ctg281_3"
                     /transl_table=11
                     /translation="MGMKDVFKRYLVFVIGLYFLAAGIVLIVRSALGTTPISSVNYVLS
                     LNSPLSLGTCTFLINMLLILGQFWVIRKNKTRQDTIEILLQLPFSFIFSAFIDFNMALT
                     SNLHPDNYGMSIALLLTGCMIQSIGVVLELKPKVAMMSAEAFVKYASRRYNKEFGKFKV
                     CFDVTLVTLAVILSLLLSQCIEGVREGSLIAACITGYIVSFLNQKIMTRKTLYKLLPVL
                     K"
     CDS             5282..5458
                     /locus_tag="ctg281_4"
                     /transl_table=11
                     /translation="MVKSRIFDNRQLLKEHPEIPHYKEEVVCFMSEYKDRSYPENERYF
                     NRELYMILVLEGRS"
ORIGIN
        1 acattaaagc tggtgttttc gattttgctt tcgagatcga cattcatctc aaggcactag
       61 ccatgaatgc gacacaggta tatcctattg cctcccgttg tggcgttttc tgtaaaacgg
      121 atattcagaa tctgatagct aaaaatgtaa gtcgtgagaa tattgcggct tccatctttc
      181 gtgcggtagc agttcaaacg gtagtgacgc tggcgcacgg ttgcgatatt acaactcccg
      241 tattgttttg tggtggtccg ttaaccttca ttccggcact gcggaaagcc ttcattgatt
      301 atttgtccct cgatgaaaaa gagatcatac tgccggccaa cggcactttg ctccctgcac
      361 taggtgctgc tctatttcat cttgataaag aggattactg taaactgtcc catttgatcg
      421 ataagattga cactacttta aacggtaacg gtaaccttgc ctcaacagga ttggaacctg
      481 tttttgcaag taatgaagaa tatgaagcat ggaaagaaag aatatcccgt cataagatga
      541 tatcttccgg cttgaaagcc ggaacacagg atgtctttat cggcatcgat tcaggttcta
      601 ctacgacaaa gattgttgta ctggatgaag attcaaggct tctgtattct tattataata
      661 ttaatggcgg caatccgata aaagctgtgg aagaggggct gaatcagttg aacgatgaat
      721 gtttgaagca gaatgccgta ctcaatataa aaggaagctg ttctaccgga tatggggaag
      781 accttatcag gtctgccttt cagcttcatt cggggattat cgaaacgatc gcccattata
      841 tggcagcgca ttatctgaac aaggatgtgt cttttatcct cgatatcggc ggacaggata
      901 tgaaggttat ttttgtgaat aatggcgtga ttgaccggat ggagattaat gaagcatgct
      961 cttccggatg tggttctttt cttgaaacgt ttgctaaatc tctggggtat tcggcacaag
     1021 agttttctct ggcagcctgc cattcggaag caccgtgtga tttgggaaca aggtgcaccg
     1081 tttttatgaa ctcaaaagtg aaacaggtac tcagggaggg ggcaacgatc aatgatattg
     1141 ccgccggact ggcatattcc gttgtcaaga attgcctgta taaggtgttg aaactgaaag
     1201 atatatctgt tttgggaaaa gatattgttg tgcagggagg caccatgcgc aatgatgcgg
     1261 tggtccgtgc cattgaactt ctctcgggag cggaagtggc aaggtgtgat acacccgaat
     1321 tgatgggagc attcggatgt gcattatatg cgatgaaaca tcagggggag tccgtatcat
     1381 tggatgagat tatcaataaa gctcaatatt cggcacgttc tttatattgc aagagttgcg
     1441 ataatcgttg tctggtcatt cgttacgagt tcgaaagcgg caaaagttat tattcgggaa
     1501 acaggtgcga gaaagttttc acgaatggag agagcagtaa ccggaaaggt ctgaatgtct
     1561 atcggcaaaa agaagagctt ttgtttcatc gcagcgcaga gattgcagca cccgagcaga
     1621 ttatcggaat cccccgatgt ctgaatatgt acgaggaata tccattctgg cacactcttt
     1681 tcacttcctg tggcatacaa gtctgtttgt ctgatccgtc caatttcaga aagtatgagc
     1741 acaatgcgcg tatggtgatg tcggacaata tctgctttcc ggctaagctg gtacatagtc
     1801 atgttcagga cttgatagaa aagaaggtcg accggatttt tatgcctttt gttattttcg
     1861 aaagaaaagg gatggaacaa aacagttata attgtccgat tgtgacaggt tattcggagg
     1921 ttatcaaaag tgtgcagtcc gaaggtatac cggttgattc ccctgcaata actttcaaag
     1981 accggaatct gttgtttaaa caatgccgtg aatatctgag cggtttgagc gtggacgatc
     2041 ggacgattca gcaagcattc caaaaagcgg aaagcgaaca gcactctttt attaatacat
     2101 tggtcgatta taacaaaaac atattgcagc agactggaaa gggagagact ttgactgtca
     2161 tgcttgccgg acgtccttat cataccgatt ctctgataca gcacaaagtc tcggatatgc
     2221 ttgcggatat gggagtgaat gtgatagcgg atgatttggt gcgtcagatg gatataccga
     2281 cggatgatgc gcattttgtt gctcaatggg cgtatacgaa ccgtatcctg aaggctgcga
     2341 aatggtgtgc gacacaagga aagaatattc agtttgtaga gatgacttcg ttcggatgtg
     2401 gaccggatgc ttttctggtg gatgaagtaa gggatttgct gatgcgtcat aacaaatcgc
     2461 tgaccttatt gaaactggat gatatcaata atatcggttc aatgaagttg agagtacgtt
     2521 ccatgattga aagcctcaaa cttgcgaatg cggatggaac ggaagacggt gtaaaggact
     2581 ttacgaccgt tcctgtttat gacaagtctt accgtgaccg taagatactc gttccttact
     2641 ttactccgtt tatttcacct ttgattcctg ctattatgaa agtagccggt tatgatgcgg
     2701 aaaaccttcc tctcagtgat aatgattcaa gtgaatgggg gttgaagtat gcgaataatg
     2761 aggtctgtta tccggctact ctgatagtcg gcgatattat aaaagcactt aaaagcggga
     2821 aatatgatat ttcaaaaata gccgtagcca tcacccaaac gggaggacaa tgccgtgcca
     2881 gcaactatat ttccttgata aaaaaggcgt tggtggatgc cggatatacg gatattccgg
     2941 taatctctat ctctgtgggc agtgacatag ataatgacca gcccgcgttc aaggtcaact
     3001 ggatgaaagt agtaccgata acttttcatg ccgttttgta tagtgattgc attgctaagt
     3061 tttattatgc gtctgtggta cgagagaaag aagcaggtgc atcggcaaag ctgcgtgata
     3121 aatacctgca actggcacct gaagtgatac tgagaagaga cataaagggg ttgaactctt
     3181 tgttgcaatc ggcaatcact gaatttaatg aggtatgtag ggctgtagat actcctaaag
     3241 tcggaattgt aggagaaatc ttcttgaaat tcaatccttt cgcacaaaaa gagatcattg
     3301 attggctgat aggccgcaag atagaagtgg ttccgccgat tctggctgac tttttcatgc
     3361 aggggtttgt gaacaggaaa gtcaatacgg attcttacat aaaaaacaga cagttgcctg
     3421 agtttattta cgactggatc tacaagtttg taaagaaaca gattgataaa ataaatgaga
     3481 cagcaagtaa gttccgttac tttgccccct tcaatgatat ctttgaagaa gcggaaggag
     3541 ctaaaagcgt tatttcgctg aatgcccaat tcggtgaagg ctggttgttg ccggctgaaa
     3601 tcgtgtctta cgcccggcaa ggggtgaaca atgtagtgag tttgcaacct tttggctgca
     3661 tagcgaatca tatcgtatcc agaggagtcg aaaaacgtat aaaaagtttc tatccggata
     3721 taaatttgct atctttagac tttgatagtg gtgtgagcga cgtaaacata acaaaccgta
     3781 tgcttttgtt tattgataat ctgaaaaatg cgacaatgaa ttatgacaac aactaataat
     3841 aatgaagatt tggagaaaaa gataattgag gctgccaaag aactatttat agagaatggg
     3901 ttcgcagaaa ccagtatgag tgatatcgct gctaaagtag gtatcaacag accggcactt
     3961 cactattatt tccgaacgaa ggataagatg tttcaggccg tttttggtaa tatcatactt
     4021 tcttttattc ccaaaataca tgatataatc attcagcagg acaaacctgt ctccgagagt
     4081 atcggtgaaa ttgtagatgc ttattataaa gtattcacgg aaaatccatg tttgccattg
     4141 ttcatggtga gggagatgca tcgtgatatg aaccatttaa gcactactat caaggagttg
     4201 cgcctggaac aatatctcca taaaatcatg gatgttttac aggaagaaat gaatgacggg
     4261 agactgaaaa cagttccttt gccattcgta ttctatactt tttatagtgc attgacagtt
     4321 cctttcctga caaagaatct cagtgcttct ttctttttgg agaaggatga ttcttttgag
     4381 gatattctgg ttcagtggaa accttatatc gtcaggcaga tggaaacgtt gctggtagcg
     4441 agctagagaa aacttatttc aatacaggta ataatttgta gagagttttc cttgtcatta
     4501 ttttctgatt caggaagctg acgatatatc cggtgatgca ggcggcgata agagacccct
     4561 cgcgaacacc ttcgatgcat tgcgaaagca ggagagagag tatgactgcc agagtgacca
     4621 gcgtaacatc aaaacagact ttgaactttc cgaactcttt gttataacgg cgggaggcgt
     4681 attttacaaa agcctctgca ctcatcattg ctactttggg tttgagttcg agtacgactc
     4741 ctatggattg tatcatgcat cctgtgagta ataaggctat tgacattccg taattgtcgg
     4801 gatgcagatt actggtgagt gccatattaa aatcaatgaa tgccgaaaag ataaaagaga
     4861 acggcaattg aagaagtatt tcaatagtgt cctgtcgggt cttatttttt ctgatgaccc
     4921 agaactgtcc cagaataagc agcatattga tcagaaacgt acaagttcct aatgataaag
     4981 ggctgttcag gcttagcaca taattgacgc tggatatagg tgtggttcct aatgcagaac
     5041 gcacaatcaa cactattccg gcagccagaa aatacaatcc gataacaaat accaaatacc
     5101 ttttaaaaac gtctttcatt cccatatctt actatcttat ataatcgtat ttttcttcat
     5161 tctataaaga agaatatgca aaggtcccgt tttttcgtct gatataagtt atccgattcc
     5221 acacagtttt tgtcatatcc gaaacaaatt gtacttttac gtcgaaaaga aggaggtata
     5281 aatggtaaaa tctcgtattt ttgataatag gcagttgtta aaggaacatc cggaaattcc
     5341 tcattataaa gaggaagtag tctgctttat gagtgaatat aaggacagga gctatccgga
     5401 aaacgaacgt tactttaatc gtgaacttta tatgatactc gttcttgaag gtcgttccg
//
