LOCUS       c00061_gnlPro..         4774 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|INPJIPBI_61.
ACCESSION   c00061_gnlPro..
VERSION     c00061_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 15:49:27
            Original ID  :: gnl|Prokka|INPJIPBI_61
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4774
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4774
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[457:3521]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      458..3521
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4774
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4774
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..209
                     /locus_tag="ctg61_1"
                     /transl_table=11
                     /translation="FRGLPVVTLFSTRLPDVYRPMGTFNRSLYSHQCCSPCFRKECDRD
                     VPCNRHIAVRDVLEALREISGKE"
     CDS             complement(458..1237)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 53.6;
                     E-value: 9.1e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg61_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.5e-31, bitscore:
                     97.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELHPTL
                     EFAERLPSLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRQGGFPDCVH"
     CDS             complement(1254..3521)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg61_3"
                     /sec_met_domain="Gly_radical (E-value: 4.2e-46, bitscore:
                     144.4, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 7.8e-283, bitscore:
                     927.6, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISIVKDLKESTALPQEWQGFKPGTWTESIDVRDFIQHNYTPYSG
                     NEEFLSGPSQRTRRLWDELKVLLKREIDNGGVLDADEEVVSSITSHKPGYIDKELEVVV
                     GLQTDAPLKRALMPFGGLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDAYTSDI
                     RAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDKLIAERRKDLKNREHSPLTDELIRL
                     REEMSEQIRALEELAQLGASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLGR
                     VSTFFDIYFTRDLEQGLITEEEVQEIIDQFVMKLRIVRFIRTPDYNNLFSGDPTWVTES
                     IGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETSS
                     VQYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGK
                     QVAPPSPRYTEEILNYDEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHD
                     PEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVD
                     DIACSLVSNFMEKLRRLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGA
                     NPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAY
                     FAATGHHINVNVLDRKTLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTFH
                     TR"
     CDS             3955..4584
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Lipase_GDSL_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg61_4"
                     /sec_met_domain="Lipase_GDSL_2 (E-value: 9.4e-25, bitscore:
                     76.5, seeds: 1564, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MERNRTVKPEYVVFGDSITHRWGGEPSGDNRALGTGKTAWHSLFS
                     SHAVTNMGFGSDYVDNAYYRLQLGELDGISPRVIIVLLGTNNLGGRKDAPRACADNLKA
                     FVSLARRKCPSSKILLLGILPREGKKLAPLIRETNKMISGLADGNSVFFANPGEEFLGE
                     DGVSPKPGLLEDGLHPSARGYDILGKSLSVLLKKMDDKYGKLPAPR"
     CDS             4629..4772
                     /locus_tag="ctg61_5"
                     /transl_table=11
                     /translation="MDEMERALEEAGHGARLAVVAAGFAGRAVVFFTLPEEVTPAILMH
                     GRI"
ORIGIN
        1 ctttccgggg gcttccggtg gtgactctgt tttccacccg gcttccggat gtttaccgcc
       61 ctatgggcac attcaaccgt tccctttaca gccaccagtg ctgttctccc tgcttccgga
      121 aggaatgtga ccgggatgtt ccctgcaacc gccacattgc cgtgcgggac gtactggaag
      181 ccttgcggga aatttccggg aaagaataat ctgggaaggg acggaatgcc gtcccccggc
      241 gttggcggaa aaaacggcgg gaatgctgcc gttgccgtaa agaatgatga aataacgaaa
      301 ggaattgcgg aaatgccgta ttccggagca attccagaaa tgcgtcttcc tttttgcatg
      361 gattctgcag tggaggggag agggcccctc atccggaaga gggggatgcc tgcgtccggt
      421 ttgctgcggt tggtggcgcc aaagcgcgaa aatccggtta atgcacgcaa tccggaaagc
      481 cgccctgccg gaaaatgccg cggacctgtt ccgccagttc tgcggacgct tccgggacgt
      541 ccttcaaggt atagtccagc ttcaattcat cccatttgaa tctccccagc tggtggaaag
      601 ggagcacgtc cacgcgttcc acgttgccca gaagcgccgc atggcgggag agttcctcaa
      661 tttcttccag attgtccgta acgccaggta ccagcacata cctcagccac acgggcttgc
      721 ggagggaggg caggcgttcc gcaaactcca gtgtagggtg cagttccccg cctgtcaggg
      781 cgcgataccg tttcggattc catgccttga tatccagcag gaccagatcc gtatccgcca
      841 gcatttcttc gcccgcattc acgcccagat ggccggaggt gtccaggcag gtgtgcaggc
      901 ccagtttccg gcagcctttc agcacggcac cggcaaaatc cggctggaag agggggtccc
      961 cgccggagag ggtgacgccg ccgcccgccg cctgcaaaaa atcccggtaa cgggctattt
     1021 ccttgaggac atcatctgcg ctcctgagcc ggcccctccg cacgtaggag gtatccggat
     1081 tatggcagta gcggcagcgc aggctgcaac cggacaggaa aaggacgaag cggatgccgg
     1141 ggccgtccac ggtgccgcag gattccacgg aatgaaccaa accggtgacg gagctttctc
     1201 cgtccggatc atggttttgt ggaaaaacct gcggcatccg cttgcagtcc gggttaacgg
     1261 gtgtggaaag tgcggttgat gacctcctgc tgctgttccc gggtcagctt gatgaaattc
     1321 acggcatagc cggaaacgcg gatggtaagc tgcgggtatt tttccgggtg atccatggcg
     1381 tcgagaagcg tcttccgatc tagtacgttc acattaatgt ggtgacctgt agcggcaaag
     1441 taggcgtcca gcagggagac gagcttgacg cggcgttcac gttcttcctt gcccagggcc
     1501 tgaggaacga tggagaaggt gtaggagatg ccgtccaggg agtcgtcata ggacagcttg
     1561 gctacggaga gcatggaggc cacggctccg ttcctgtccc ttccgtgcat ggggttggct
     1621 ccgggggcga acggttcccc ggctcggcgg ccgtccggcg tgttgcccgt ctttttgccg
     1681 tacaccacgt tggaggtgat cgtcaggatg gactgggtgg gcagggaatt gcggtaagtg
     1741 tggagcctgc gcagtttttc catgaaattg ctgaccaggc tgcatgctat gtcgtccacg
     1801 cgcgggtcat tgttcccgta acaggggaat tccccttccg tcttgaagtc cacaatcagc
     1861 ccttcttcat tgcggatggc ttttacggtg gcatacttga tggcggacag ggagtccgcc
     1921 gccacggaaa gcccggcgat tcccgtagcc atcgtgcgca gaatttccgg atcatgcagc
     1981 gccatttcaa tgcgttcata gcagtatttg tcgtgcatgt agtggatgat gttcagcgcg
     2041 tcaatgtaag ttttggcgag ccagtcctgc atcttgtcgt acagggccat cacctcatca
     2101 tagttgagaa tttcctccgt gtagcggggg gagggcgggg ccacctgctt gcccttgagt
     2161 tcatccacgc cgccgttcag ggcgtagaga aggcacttgg cgaggttggc gcgcgctccg
     2221 aagaactgca tctgcttgcc gatgcgcatg gcggacacgc agcatgcgat gccgtagtcg
     2281 tcgccccaat gcgggcgcat caggtcgtcg ttctcgtatt ggacggagga cgtttcaatg
     2341 gaaaccttgg cgcagaagct tttgaaggct tccggcaggt tccgggacca cagaacggtc
     2401 aggttcggtt ccggagccgg ccccaggttg tacagggtct gcagcatgcg gaaggaactg
     2461 cgggtgacga gggtgcgccc gtcctctccc atgccgccga tggattccgt cacccaggtg
     2521 gggtctccgg agaacaggtt attgtaatca ggcgtgcgga tgaaacggac gatgcgcagc
     2581 ttcatcacga actggtcgat gatttcctga acttcctctt ccgtgatgag cccctgttcc
     2641 aggtcgcggg tgaaataaat gtcgaagaaa gtggaaacgc ggcccaggga catggccgct
     2701 ccattctgtt ccttcacggc ggctaggtag cccaggtacg tccattgcac ggcttcgcgg
     2761 gcgtttgcgg cggggcgagt gaggtcgcag ccgtaggagg cgcccagctg ggcgagttct
     2821 tccagggcgc gtatttgttc gctcatttct tcgcgcagac ggatcagctc atccgtaagg
     2881 ggggaatgtt cacggttttt gaggtccttg cggcgttcgg caatcagctt atccgtgccg
     2941 tacagggcca cccggcggta gtccccgatg atgcggccgc ggccataggc gtccggaagg
     3001 ccggtaataa tcccggcgga acgggccgcc cggatatcgg aagtgtaggc gtcgaaaacg
     3061 ccttcattgt gcgtcttgcg tatctttttg aaaatgtctg cggttttctc gcacatcttg
     3121 aacccgtagg attccagggc ctgttgggcc atgcgcaagc cgccaaaggg catcagggcc
     3181 cgtttcaggg gggcgtccgt ctggaggcct acgaccactt ccagttcctt gtcaatgtag
     3241 ccgggcttgt gggatgtgat ggaggatacc acttcttcat cagcatccag cacgccgccg
     3301 ttgtctattt cccgtttcag caggactttc agctcgtccc ataaacgacg cgtgcgctgg
     3361 gaggggccgg aaagaaactc ttcattgccg gaatagggcg tgtagttatg ctggatgaaa
     3421 tcccgtacgt caatggactc cgtccatgtt cccggcttga atccctgcca ttcctgcgga
     3481 agggcggtgg actccttcaa gtcttttact atgctgatca taaatagaat gtagtgaaga
     3541 agactggaga aaaacgttcc gcagcccctg aaaggctgga aacggcaaaa ctccgcgccg
     3601 cgtaattata tcgtgaattc cgggggacaa caaggctaat tctttgcgaa gagaacgcgg
     3661 gtgcgctggc tttggcatgt ttcacctgtc tgtccgttgc ggagcggaaa atgaggggaa
     3721 catgcgattg cggaaaagtg acggaagatt atttctcttt actttcctaa tctctattgc
     3781 agtatctcaa cgcttaccgt tatgaaatct tatgcatgcc tttgtttatt gctggcggga
     3841 gttttttgcg ttgcttgttt ctccggtgcc tgtgctgatg aattgccgcc cacgctgaag
     3901 ccccaggtga acccgcgggc cgtgtatgac tagaaaaaac gtcatgaagc agtgatggag
     3961 cggaaccgga ccgtcaaacc ggaatatgtc gtctttggtg acagcattac ccaccgctgg
     4021 ggcggcgagc cttccgggga caacagggct ctcggaacag gaaaaaccgc ctggcatagc
     4081 ctgttttctt cccatgcggt aaccaatatg gggtttggtt ccgattacgt ggataatgcc
     4141 tattaccgct tgcagctggg agagctggac ggcatttctc cgcgggtcat catcgtgctg
     4201 ctgggaacca ataatcttgg cgggcggaag gatgctcccc gggcctgcgc agacaatctc
     4261 aaggcttttg tgagcctggc gcgccggaag tgtccctctt ccaaaatcct gttgctgggc
     4321 attctcccca gggagggaaa aaagctggct cccctgatta gggaaaccaa taaaatgata
     4381 tccggcctgg ctgacggaaa cagcgtgttt ttcgccaatc ccggcgagga attcctgggg
     4441 gaggacggag tgtccccaaa acccgggctg ctggaggatg ggctgcatcc cagtgccagg
     4501 gggtacgaca tactggggaa gtcgttgagc gtattgctga agaaaatgga cgacaagtac
     4561 ggcaagctgc ccgctccccg gtagatgaat acgggggagg gcgttcctgt tccgggagag
     4621 gccgggggat ggatgaaatg gagcgcgcct tggaagaggc cggacacggt gctcgcctgg
     4681 ccgtggttgc ggcaggattt gccggacggg cagtggtctt ttttactctg ccggaggaag
     4741 tgacgcctgc gatcttgatg cacggcagaa tttg
//
