LOCUS       c00412_gnlPro..         3044 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AEHABHJB_412.
ACCESSION   c00412_gnlPro..
VERSION     c00412_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 09:43:19
            Original ID  :: gnl|Prokka|AEHABHJB_412
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3044
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..630)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 76; E-value:
                     1.2e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg412_1"
                     /sec_met_domain="Radical_SAM (E-value: 2.4e-32, bitscore:
                     99.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINVHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDIRLLGESLGKYRQIQRVEILPYHRLGVHKYEVMGWD"
     protocluster    1..3044
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2873]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2873
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3044
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3044
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(627..2873)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg412_2"
                     /sec_met_domain="Gly_radical (E-value: 6.3e-37, bitscore:
                     113.6, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1171.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLIEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSHPATSAQEAVQWVYMAYLAAIKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKINETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGETL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTNPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNEIGLTEGFDTEGSFPCFGNNDDRVDHLGVDLVYYFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLN
                     REMLEDAMEHPEKYPQXXXXXXHPRFRLCRELREVEPRASVGSHQPFIPRKNVI"
ORIGIN
        1 atcccatccc atgacttcgt atttgtgaac gcccagacgg tggtaaggaa gtatttccac
       61 ccgttgtatc tgccggtatt tgccgagact ttcacccagc aggcggatgt cttcttccga
      121 atcgctgtaa ccgggaacca gtacataacg cagccagaag gggtgttcat gttcttcgag
      181 ccaggcagcg gtgcggagtg tctgttcgtt gcttcgtccc gtcagcgtac ggtggcgttc
      241 gggattgaat tgcttgatgt ccagcagcac gaggtcagtc aggctaaaga gctcttccac
      301 cttttcattc cagatgcctc cgttactgtc cagacagata tggatgccgt tcgccttcag
      361 ctccttgaaa agaggaatca gcgcttctgc ttgcagggta ggctcaccgc cggagaaagt
      421 aatacctcct ttcttcccga agaatgcctt ttggctgata gccatttgca gaatctcttc
      481 cggagtggtg ggagtgcctc cttttacatc aatcgtatcc ggattggcac aatacagaca
      541 acggaacgga catccttgca ggaaaacgac gagccggaga cccggcccgt cgaatgttcc
      601 catactttcg taggagtgta catttatcat attacattct ttcgtggaat gaacggctga
      661 tgacttccaa ctgatgctcg cggctcaact tcacgaagtt cacggcatag ccggaaacgc
      721 ggatggnnnn nnnnnnnnnn nnnttgcgga tacttttcag gatgttccat ggcatcttcc
      781 agcatttcgc ggttcagtac gtttacgttc aaatggtggg cacctttggt gaagtagccg
      841 tccatcatgg ttaccaggtt ctctacacgt tcttccggtg tcgggcccag tgatttagga
      901 acaatggaga acgtattgct gatgccgtcc tgcgagtcgc ggtagcgcaa tttggctacg
      961 gagctcaagg aagcgatggc accgttcttg tcacgtccgt gcattgggtt ggcacccgga
     1021 gcaaaggcaa cacctttggc gcgtccgtcc ggagtggcac cggtcttctt gccgtacatc
     1081 acgttggagg tgatagtcag caaggaaagg gtgggacggg cattcttgta gacaggcagc
     1141 tttttcagtt cttcgctgaa atagtaaacg aggtctactc ccaggtggtc cacacggtcg
     1201 tcattattgc cgaagcaggg gaatgagcct tcagtgtcga aaccttcggt caggccgatt
     1261 tcgttgcggc gtgcagtgac tttggcatat ttgatggcgg acagtgagtc tatggcgatg
     1321 gaaaggccgg caacaccgta tgccagattg atgcgtgggt tggtatcgat gaatgccatc
     1381 tgggccttct cataataata cttatcatgc atgtagtgga tgatattcat ggcctcgttg
     1441 tagacacggg ctatttcagt cagtaccttc ttgtagttgg ccataacttc ctcaaacttc
     1501 agtgtctcgc cggtcaatac gggaatacct ttcaccatga cagttccggt gttttcgcaa
     1561 cggccaccgt tgatggcaag cagcagggcc ttggcaaggt tggcacgtgc gccgaagaac
     1621 tggatttgct tgccgatggc ttggtaggat acacagcagg caatgccgta atcgtcgcag
     1681 ttgcgcactt cgcgcatcag gttgtcgttt tcgtactgga tggagctggt atctacggaa
     1741 actttggcgc agaaatcctt gaagccttcc ggcagttcgg gactccacag tacggtcaag
     1801 tttggttccg gagaaggacc gaggttgtat agcgtctgca agaagcggaa agaagtcttg
     1861 gtgactttca cgcgtccgtc attgaagcgt cctccgatgg cttcggtcac ccaagtcggg
     1921 tcaccggcaa agatatcatt gtacgactgc atacgcagat ggcgtaccat gcgcagcttg
     1981 atgacgaact ggtctatcag ttcttgcgca aacgtctcat tgatttttcc gtgagccaga
     2041 tcgtattcaa tgaatatatc gaggaacgaa gatacgttac ccaatgacat ggcagcaccg
     2101 tcttgctctt tgatggcagc aagatatgcc atgtataccc attgcacggc ctcttgagct
     2161 gatgtagccg gatggctgag gtcgagtccg taatattcgc ccataacctt gatgtccttc
     2221 aaagctttga tttgttccgc cacttcttcg cgtagacgga tgcgtgcttc ggtcatcggg
     2281 ccggtgaggt tgcgcaagtc ctcctgcttg gcttcaatca gacggtcgat gccgtagaga
     2341 gccaatcggc ggtagtcgcc gatgatacgt ccgcgggcat agttgtcggg caatccggta
     2401 aggaatccca gtgagcggaa ggaacggatt tcttcggtat atacatcgaa tacgccgtcg
     2461 ttatgtgttt tgcggtaatg ggtgaagatg tcttttactt tatcgtccac ttccactccg
     2521 ttttcacggc atgcttttgc taccacgttg atgcctccga aaggtttgat ggatcgtttc
     2581 aacagttcat cagtctgcaa accgacaata agttcattgt ccttatcaat atacccggct
     2641 ttatgagaag tgatggtaga tacggtggta ttgtccagtg aacggacgcc attgttgttc
     2701 ctttcttctt cgagagcctt cagacagatg tcccagatac gtttggtacg ttcggtaggt
     2761 ccctgcaaga aagatgcatc cccggtatag ggtgcgatgt ttttactgac gaaatcactg
     2821 acattgattt ctttattcca aagcccattg gtaaaggttt tgttcaattc cataaaattt
     2881 gtttttgtta gtaggttaga taatgttatc ctttagagcg cacaaaaata ctgaataaat
     2941 tccgtaattt tataagagat acttctcttt ttatgttgaa cagcataaaa taactattat
     3001 taggtagtga atgtccgggg gagtagagat gttaatactt tcta
//
