LOCUS       c00222_gnlPro..         7402 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DKHPHLJG_222.
ACCESSION   c00222_gnlPro..
VERSION     c00222_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 16:39:46
            Original ID  :: gnl|Prokka|DKHPHLJG_222
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7402
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7402
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3542:7171]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3543..7171
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..7402
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7402
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             80..1903
                     /locus_tag="ctg222_1"
                     /transl_table=11
                     /translation="MNKTILLVVFCFSLFVSGYSQGIRFDSYPSHPRLLVKKGEEQKIK
                     SDLALNPEMLRLHNYLLGQCDDLITTPVLAYKKEGKRLLAVSREALRRIFYLSYAYRMT
                     GEDKYRLRAEQEMIAVCSFTDWNPSHFLDTGEMTMAVAIGYDWLFDRLSPDARRLVQEA
                     ILNKGFAPSRDKRYNWFLRATNNWNQVCNAGLVYGALAVLEDHPEEAREIIERALSTVG
                     LTLKGYGPDGAYPEGYNYWGYGTTFQVLLNAALESALGTDGGLSSAEGFLASARFMQFM
                     TGTTGKCFNFSDSRESVFALPAMYWFADKLQDPTLLWNEKDMLSRAELSFSDEESRFLP
                     LIPIYGSQYKMNELVPPAMKLWAANGQTPVVLVRTGWENGEGFYLGIKGGTASTSHAHM
                     DAGSFVFDASGVRWVQDLGMQEYYSLEKENVDLWNMSQNGQRWNVFRYNNLAHNTLTVN
                     GKYHDVNGFVPVTNIYSEAHKLGASLNMTDLFGGELENAVREVALINEKYLSVIDRVKA
                     GSQPATVRWTIVTGAIPRVLDSHMVELTKDGKCMQLIVDSPSVTSVSVLDNVSKNSYDA
                     SNEGTCRIVFDVSLESGQESDIKVRLVPAAD"
     CDS             complement(1907..3304)
                     /locus_tag="ctg222_2"
                     /transl_table=11
                     /translation="MLQRKMLSFLFMMIFIPSVRAQWKDSTRFINKEHEFETTYNKISE
                     NKVYQMLRVPVPLFAISAITYGQGDKFRTLRNTHTPNFHYRYDDFLQYTPLLAVYGMKL
                     GGVESRSSWPRMIVSNAFSATIMAATVNSMKYTIKRERPDGSKNNSFPSGHTATAFMAA
                     TIMHREYGLTRSPLYSIGGYTVATATAFSRQLNNRHWLSDVLAGAGIGILSTELGYFLA
                     DLIFKDKGLNLPDKLRESAPKGGRPSFLEFAVGYSIINGSIEVGKNIRLSSPGATNVSV
                     KGGYFFNPYFGIGGEITALASPMAFDMSLYTGTTPSTKYDITRIHADPTGVFTFTAGPY
                     FSLPVASKLLLGTKFQAGYSYLVKNEVEMDLQEKHPPYGTINNIAFMKGNGSSNYVLRT
                     GISATGIVSRNLGIRMFVDYDYTFLKAPYEILTSLRDSQPVYGESHLSKHKIHYFTIGA
                     TVTALFW"
     CDS             3543..4268
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg222_3"
                     /sec_met_domain="SucD_FumR (E-value: 1e-76, bitscore:
                     245.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWLLNSSIGKKLIMSVSGLFLVLFLLFHLSMNVAAAFSGEAYNMI
                     CSLLGANWYAVAATLVLAAGVVIHFVYAVLLTLQNRKARGNDRYAINARPKGVEWASQN
                     MLVLGIIIILFMVLHFSQFWYKMMFAELLGNHMVTLCDGTTTVSPQDGAAFINYYFQGN
                     ILNTVLYLVWYVALWFHLTHGFWSAIQTIGWSNTIWMNRWICISKIVATVICVVFAIIT
                     IVFYLNGVGGDYVAVCAGH"
     CDS             4302..4421
                     /locus_tag="ctg222_4"
                     /transl_table=11
                     /translation="MTQIDSKIPQGPLAEXXXXSSGILREITPLWRCLLNSKL"
     CDS             4445..6388
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 190; E-value:
                     2.5e-58)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg222_5"
                     /sec_met_domain="FAD_binding_2 (E-value: 2.4e-59, bitscore:
                     190.0, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 4.5e-39,
                     bitscore: 122.1, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIKIDSKIPEGPVAEKWTNYKAHQKLVNPANKRRLDIIVVGTGLA
                     GASAAASLGEMGFRVFNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKG
                     GDYRAREANVYRLAEVSNSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQT
                     GQQLLLGAYSALSRMIGLNKVKMYTRHEMLDVVKVDGRARGIIARNLITGQIERFSAHA
                     VVVATGGYVNTFFLSTNAMASNGSAAWQCYKKGAYFANPCMVQIHPTCVPVKGDYQSKL
                     TLMSESLRNDGRIWVPKKLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDV
                     ASRAAKERCDAGFGVNNTGLAVFLDFSDAINRLGKDVVKAKYGNLFDMYEEITNDDPYE
                     TPMMIYPALHYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYF
                     VLPYTIQNYLSDQIQVPRFSTDLPEFAEAEKGIKDRIKKLMSIKGKESVDSIHKKLGHI
                     MWEHVGMARDAKGLQEAIQMLKELKKEFWSNVYIPGEADNLNVELDKALRLADFIEIGT
                     LMAHDALDRAESCGGHFRTEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNY
                     QFVVPQTRNYKK"
     CDS             6416..7171
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg222_6"
                     /sec_met_domain="Fer2_3 (E-value: 4.1e-27, bitscore: 83.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 1.3e-12, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.4e-06, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDIKVTLKVWRQKSPKDKGQFETYQIDKINQSVSFLEMLDILN
                     ERLVSEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFNDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFVSVNTGGVPDANSIAIPRADAEMAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
ORIGIN
        1 ggtctttatt tttattagac gcgcttataa atctcaattt ttatgtaagt ttgtaactcc
       61 aattaaatat aatacctgta tgaataaaac tatcctgctt gttgtgtttt gcttttccct
      121 gttcgtttcg gggtattcac agggaatacg ctttgattct tacccttctc atccccggtt
      181 gctggtaaag aaaggagagg agcaaaagat taaatccgat ctggctctta atccggagat
      241 gctccggttg cataactatc tacttgggca atgcgacgat ctgatcacga cgcctgtgct
      301 ggcatataaa aaagaaggga aacggttgct ggccgtatcg cgtgaagcct tgcggcgtat
      361 cttctatctt tcatatgctt accggatgac aggagaggat aagtatcgtt tgcgggcaga
      421 gcaggaaatg attgccgtgt gttcgtttac cgattggaac ccgtcccatt ttctggatac
      481 gggcgaaatg actatggcgg ttgcaatcgg ttatgactgg ttgttcgatc ggttatctcc
      541 ggatgcccgg aggctggtgc aggaggcgat tctgaataaa ggttttgccc cgtcgaggga
      601 taaaagatat aactggtttc tgagagcgac caataactgg aaccaggtct gtaatgccgg
      661 actggtttac ggtgcacttg ccgttttaga agatcatccg gaagaggccc gtgaaatcat
      721 tgaacgtgct ttatctacag tcggcttgac tttgaagggc tatggtcccg atggcgctta
      781 tcccgaagga tataattatt ggggatacgg gactaccttc caggttcttt tgaatgccgc
      841 gttggaatcc gccttgggga ctgacggcgg tctctcttcg gctgaaggtt ttctggcttc
      901 tgcccgtttt atgcagttta tgaccggaac gaccggtaaa tgctttaact tcagcgattc
      961 ccgtgagagt gtctttgctt tgccggctat gtactggttt gccgacaagc tgcaagaccc
     1021 tacactgctt tggaatgaaa aggatatgtt gagtcgggcg gagttgtctt tttcggatga
     1081 ggagagccgt ttccttccgt tgattccgat ttatggttca caatataaaa tgaatgaact
     1141 ggtgcctcct gctatgaaat tgtgggcggc taacggacag acgcctgtcg tgctggtccg
     1201 tacgggttgg gagaatggag aaggtttcta tctgggaata aaaggaggaa cggcttcgac
     1261 cagccatgcc catatggatg ccggatcgtt tgtgttcgat gcttcgggtg ttcgctgggt
     1321 gcaggacctg gggatgcagg aatattattc gctggaaaag gaaaatgtag atttatggaa
     1381 tatgtcgcag aacgggcagc gctggaatgt gttccgttat aataacctgg cgcataatac
     1441 actgactgta aacggtaaat accatgatgt aaacggtttt gtacctgtga caaatatcta
     1501 ttcggaagcg cataaactcg gagcttctct gaatatgacg gacttgttcg gcggagaatt
     1561 ggaaaatgcc gtccgtgaag ttgctctgat caatgaaaaa tatctgtcgg tgattgatcg
     1621 tgtaaaagct ggcagtcagc cggcaactgt cagatggacg attgtaaccg gggccattcc
     1681 ccgtgtattg gacagtcata tggtggaact gacaaaagac ggcaaatgta tgcaactgat
     1741 cgttgatagt ccatccgtaa cttctgtgtc cgtccttgat aatgtatcga agaatagcta
     1801 tgatgcttcc aatgagggaa cctgccgtat cgttttcgat gtaagtctgg aaagcgggca
     1861 ggagagcgat atcaaagtcc ggctggttcc tgctgctgac tgataattac cagaacagcg
     1921 ccgttacggt tgcccctatt gtaaaatagt gtattttgtg cttgcttaaa tggctctcgc
     1981 cgtagactgg ttggctgtct ctgaggctgg ttaagatttc gtaaggtgct ttcaggaacg
     2041 tataatcgta atctacgaac atacggatac ccaggttgcg gctgacgatc ccggtcgctg
     2101 aaatgcctgt gcgtaaaaca tagttgctgc taccgttgcc tttcataaaa gcaatattat
     2161 ttatggttcc gtatgggggg tgcttttcct gtaagtccat ttccacttca tttttcacca
     2221 gataggaata tccggcctgg aactttgttc ctaaaagcaa tttactggct acaggtaacg
     2281 agaaataagg ccctgccgta aaagtaaaaa caccggtcgg atcggcatgg atgcgggtga
     2341 tgtcatattt ggttgaaggg gttgttcctg tataaagcga catgtcgaat gccatcgggc
     2401 ttgccagcgc tgttatttcg ccgccgatac cgaaataagg attgaagaag taaccgcctt
     2461 ttacgctgac attggttgct cccggcgagc tcaggcgtat atttttacct acctcgatac
     2521 ttccgttgat gatactgtat cctacggcga actccaggaa agaagggcgc cctcctttgg
     2581 gggcagactc cctgagcttg tcagggagat tcaagccttt gtccttgaag atcaggtctg
     2641 ccagaaaata ccctaactct gtggataaga tgccgatacc ggccccggcc agtacgtcgc
     2701 tcagccagtg gcggttgttc agttgccggc tgaaagcggt ggctgtggcg actgtatatc
     2761 ccccgatact atataacggg ctgcgggtca gaccatattc ccggtgcatg atggtagcag
     2821 ccatgaaagc ggttgccgtg tgtcccgaag ggaatgagtt gttctttgag ccgtcggggc
     2881 gttcgcgttt gatcgtatat ttcatgctgt tgacggtagc ggccatgatc gttgccgaaa
     2941 aagcgttcga tacgatcatc cggggccatg aactacgact ttccacaccg ccgagcttca
     3001 tcccgtaaac agcaagcaac ggagtatatt gcagaaaatc atcgtatctg taatggaagt
     3061 tgggcgtatg cgtattgcgc aatgtccgga atttatcgcc ctgcccatat gttatggctg
     3121 aaatggcgaa aagaggaaca gggacacgca gcatctggta taccttattc tctgatattt
     3181 tgttgtaagt ggtttcaaat tcatgttctt tatttatgaa tctcgtacta tctttccact
     3241 gagcacgaac agacggtata aaaatcatca taaataagaa gctcagcatt ttacgttgta
     3301 acatacgatc ccctgattat gccacgaaga tataaaaatg ccggttagta ctggttacat
     3361 tttcgttaat ttatttattg aataggattt accgtttgtc cctcctgaaa tgtataggtt
     3421 tgattggcga aatatttctt taatcgtttg cgtgatacgt gtatttagca tacctttgcc
     3481 tgataattga gtatattgat atgaatttct aaaaacaatt aaataactta aattattatc
     3541 ttatgtggtt acttaattct tctatcggga agaagctgat aatgagtgta tcaggccttt
     3601 ttcttgtttt gttcttattg ttccacttgt caatgaatgt ggcggctgct ttctcgggag
     3661 aagcctataa catgatttgt tcattgttgg gagcaaattg gtatgccgtg gcagcaacat
     3721 tggtgctggc agcaggcgtt gtgattcact ttgtgtatgc tgttctcctt acactgcaaa
     3781 accgtaaggc tcgcggtaac gaccgttatg caattaacgc tcgtccgaaa ggtgtggaat
     3841 gggcatctca gaacatgctg gtactgggta ttatcattat cctgttcatg gtgttgcact
     3901 tcagccagtt ctggtataag atgatgttcg ccgaactcct aggcaatcac atggtaacac
     3961 tttgcgacgg aacaacaacc gtatctcctc aggacggtgc agcgtttatc aactactatt
     4021 tccagggtaa tatcctgaat acagtgctgt atctggtatg gtacgtagct ttgtggttcc
     4081 acctgactca cgggttctgg agcgctattc agactatcgg ttggagtaac acgatctgga
     4141 tgaaccgttg gatctgcatt tccaagattg tagcaaccgt tatttgcgta gtgtttgcta
     4201 tcattactat cgttttctac ctgaatggtg taggtggtga ctatgtagct gtttgtgcag
     4261 gacattaatt taaggtgtaa caatattgaa aataaatcat tatgactcaa atagattcta
     4321 aaatccctca gggcccgttg gctgagannn nnnnnnntag tagtggtatt cttcgtgaaa
     4381 tcactccttt gtggcggtgc ttgctaaata gtaaattgta aatgattaaa ttgtaaataa
     4441 tattatgatc aagatagatt caaaaattcc agaaggcccg gtggctgaga aatggaccaa
     4501 ctataaagct caccagaaat tggtgaaccc cgctaataaa cgccgtttgg atatcatcgt
     4561 tgtgggtacg ggtctggcag gtgcttctgc tgctgcatcc ctcggtgaaa tgggtttcag
     4621 agtattcaac ttctgtattc aggactctcc gcgccgtgca cactctatcg ctgcacaggg
     4681 tggtatcaat gctgcaaaga attatcagaa tgatggtgac tctgtttacc gtctgttcta
     4741 cgatacaatc aaaggtggtg actatcgtgc ccgcgaagca aacgtttatc gtctggctga
     4801 agtttccaac tctattatcg accagtgcgt agcacagggt gttcctttcg ctcgcgaata
     4861 cggtggtctg ctggataacc gttcattcgg tggtgctcag gtatcccgta cgttctatgc
     4921 ccgcggtcag accggacagc agttgttgct gggtgcttac tctgctttga gccgtatgat
     4981 cggtttgaac aaagtaaaga tgtatacccg ccacgaaatg ctggacgttg tgaaggtgga
     5041 tggccgtgcc cgcggtatca tcgcacgtaa cctgatcaca ggtcagatcg aacgtttttc
     5101 tgctcacgca gtagtggtgg caacaggtgg ttatgtaaat actttcttcc tgtctactaa
     5161 cgcaatggct tccaacggtt ctgctgcatg gcagtgctac aagaaaggtg cttacttcgc
     5221 taacccttgt atggtgcaga tccacccgac ttgtgtaccg gtgaaaggtg actaccagtc
     5281 taagctgact ttgatgtctg aatcattgcg taacgacggt cgtatctggg ttccgaagaa
     5341 actggaagat gcaaaagcat tacaggccgg aactaagaaa gggaaagaca ttcctgaagc
     5401 agaccgcgac tactatctgg aacgccgtta tccggcattc ggtaacctgg ttccgcgtga
     5461 cgtggcttca cgtgccgcta aagaacgttg cgacgccggc ttcggtgtga acaacacagg
     5521 tctggccgtg ttcctcgact tctccgacgc catcaaccgt ctgggtaaag atgttgtaaa
     5581 agctaaatat ggtaacttgt tcgacatgta cgaagaaatt accaacgacg atccatacga
     5641 aactccgatg atgatttatc ctgcattgca ctactcgatg ggtggtctgt gggttgacta
     5701 tgaattgatg acttctattc cgggtctgtt cgctatcggt gaagctaact tctctgacca
     5761 cggagctaac cgtctgggtg cttctgcgtt gatgcaggga ttggccgatg gctactttgt
     5821 attgccttat acaattcaga actatctgtc agaccagatc caggttccac gtttcagcac
     5881 cgacctgcct gagtttgcag aagctgaaaa ggggatcaaa gaccgcatca agaaattgat
     5941 gagtatcaag ggtaaagagt cggtagattc tatccataag aaactgggtc atatcatgtg
     6001 ggaacatgta ggtatggcac gtgatgcaaa aggtttgcag gaagcgatcc agatgttgaa
     6061 agagctgaag aaagagttct ggagtaatgt atatattccg ggtgaagccg ataacctgaa
     6121 cgtagagttg gataaggcat tgcgtttggc tgactttatt gagattggta cattgatggc
     6181 acacgatgca ttggaccgtg ccgaatcttg tggaggacac ttccgtacag agcaccagac
     6241 cgaggaaggc gaagccctgc gtcatgacga taagttcatg tacgtatctt gttgggaata
     6301 ccagggtgaa gacaaagatc cggttatgct gaaggaagat ttgaactatc agttcgttgt
     6361 tcctcaaaca cgtaactaca agaagtaata atcatcaatc acaaaaatta gaatcatgga
     6421 taaagatata aaagtaacac ttaaagtttg gcgtcagaaa agtccgaaag ataaaggaca
     6481 atttgagact taccaaatag acaaaatcaa tcagagtgtt tctttcctgg aaatgttaga
     6541 cattttgaat gaacgtttgg tcagtgaagg taaagagccg atcgtattcg atcacgactg
     6601 tcgtgaaggt atctgcggta tgtgttcttt gtatgtaaac ggacatccgc acggacctgc
     6661 aaccggcgca actacttgcc agctttatat gcgtcgcttc aatgatggcg aaactattac
     6721 aatcgaaccg tggcgttctg ccggtttccc ggtaatccgc gacttgatgg tagaccgtta
     6781 tgcttacgat aaaattatcc aggcaggtgg tttcgtatca gtgaacacag gtggtgtacc
     6841 tgatgccaat tcaatcgcta ttcctcgtgc agatgctgaa atggctatgg atgctgctgc
     6901 atgtatcggt tgcggtgctt gtgctgctgc ttgtaagaac ggttcggcta tgttgttcgt
     6961 ttctgcaaag gttagccagt tggctctgtt gcctcagggt aaggtagaag ctgctcgtcg
     7021 tgcgaaagca atggttgcca agatggacga actgggcttc ggtaactgta ccaacacgcg
     7081 tgcttgcgaa atggaatgtc cgaagaacat ctctgtcagc aacatcgctc gtttgaaccg
     7141 cgagttcatt tgtgctaaat tgcaagacta atatacatta tatatgtatg tccggagagg
     7201 cgttgcttat ggcagcgcct cttctttttt gcccctattt taacatttct gaagtcatag
     7261 ataaatatct tgaatgttgt tagtgtttct tatatgctat acaacactaa ttagcatttt
     7321 aactattgac aagttggaga atacctgtat ctttgtactg tggttttttc ataatagtat
     7381 tagatttaag gttaacaaag tt
//
