LOCUS       c00190_gnlPro..         4789 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EODDBFHD_190.
ACCESSION   c00190_gnlPro..
VERSION     c00190_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 09:46:39
            Original ID  :: gnl|Prokka|EODDBFHD_190
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4789
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4789
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1350:4414]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1351..4414
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4789
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4789
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..1102
                     /locus_tag="ctg190_1"
                     /transl_table=11
                     /translation="TIEVNRGLETLIRESVLDVFWMHHRWKSVDRFAPQDKKTAALLEN
                     MELKPYRILVAVPRALDEALATVPLIRALKTVRCDMQVNIVCPSAQLGIWKTVPEVTHV
                     LPHDSLKQLREALAADEFYNDGPLDLGVMLDGDMETLKALEPYGPMMFSGLDTHPGARK
                     YKFRVKAPVLRAAPPAHRVQLYLQLGVLHGLDVATPSLFPVKKAAPAEDAPILIAPFSR
                     LGSASEWSREQWRELVSLLPGRTVLAALEEDRERAASLAEHLNVELAVGAPEALFPVMD
                     GAAAAVAVDGDFPSLCSFRGLPVVTLFSTRLPDVYRPMGTFNRSLYSHQCCSPCFRKEC
                     DRDVPCNRHIAVRDVLEALREISGKE"
     CDS             complement(1351..2130)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 54.5;
                     E-value: 4.9e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg190_2"
                     /sec_met_domain="Radical_SAM (E-value: 4.2e-32, bitscore:
                     99.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGQLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRRGGFPDCVH"
     CDS             complement(2147..4414)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg190_3"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-46, bitscore:
                     145.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.6e-282, bitscore:
                     923.5, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISIVKDLKESTALPQEWQGFKPGAWTESIDVRDFIQHNYTPYSG
                     NEEFLSGPSQRTLRLWDELKVLLKREIDNGGVLDADEEVVSSITSHKPGYIDKELEVVV
                     GLQTDAPLKRALMPFGGLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDAYTSDI
                     RAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDKLIAERRKDLKNREHPPLTDELIRL
                     REEMSEQIRALEELAQLGASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLGR
                     VSTFFDIYFTRDLEQRLITEEEVQEIIDQFVMKLRIVRFIRTPDYNNLFSGDPTWVTES
                     IGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETSS
                     VQYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGK
                     QVAPPSPRYTEEILNYDEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHD
                     PEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVD
                     DIACSLVSNFMEKLRRLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGA
                     NPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAY
                     FAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTFH
                     TR"
ORIGIN
        1 cacgattgaa gtgaacaggg ggctggaaac gttgatccgg gaatccgtgc tggatgtgtt
       61 ctggatgcac caccgctgga aatccgttga ccgatttgcc ccgcaggata aaaaaacggc
      121 tgcgctcctg gaaaatatgg agctgaagcc gtaccgcatt ctggtagccg ttcccagggc
      181 gctggatgaa gccctggcga ctgttcccct gattcgtgct ttgaaaaccg tccggtgcga
      241 catgcaggtg aacatcgtct gcccgtctgc ccagctgggg atatggaaga cggtgccgga
      301 agttacccat gtgctgccgc acgactccct gaagcagctt cgggaagctt tggcagcaga
      361 tgaattttac aatgacggac cgctggatct gggcgtcatg ctggatggag atatggaaac
      421 cctgaaagcg ctggaaccgt atggccccat gatgttttcc gggctggaca cgcatcccgg
      481 tgcccggaag tacaagttcc gggtaaaggc ccccgtcctg cgcgccgccc ccccggcgca
      541 ccgggtgcag ctttatctgc aattgggggt gctgcatggg ctggacgtcg ccaccccgtc
      601 tttgttccct gtgaaaaaag cggccccggc ggaagacgcc cccattttaa tcgctccgtt
      661 ttcccgcctg gggagcgcca gtgaatggag cagggagcag tggagggagc tggtttccct
      721 tctgccggga cggacggtgc tggcggctct ggaagaagac cgggagcgtg ccgcctccct
      781 ggcggaacat ttgaatgtgg agctggcggt gggtgcgccg gaggctctct ttcccgtcat
      841 ggacggcgct gcggcggcag tagccgtgga tggcgacttt ccgtccctct gttctttccg
      901 ggggcttccg gtggtgactc tgttttccac ccggcttccg gatgtttacc gccctatggg
      961 cacattcaac cgttcccttt acagccacca gtgctgttct ccctgcttcc ggaaggaatg
     1021 tgaccgtgat gttccctgca accgccacat tgccgtgcgg gacgtactgg aagccttgcg
     1081 ggaaatttcc gggaaagaat aatctgggaa gggacggaat gccgtccccc ggcgttggcg
     1141 gaaaaaacgg cgggaatgct gccgttgccg taaagaatga tgaaataacg aaaggaattg
     1201 cggaaatgcc gtattccgga gcaattccag aaatgcgtct tcctttttgc atggattctg
     1261 cagtggaggg gagagggccc ctcatccgga agagggggat gcctgcgtcc ggtttgctgc
     1321 ggttggtggc gccaaagcgc gaaaatccgg ttaatgcacg caatccggaa agcctccccg
     1381 ccggaaaatg ccgcggacct gttccgccag ttctgcggac gcttccggga cgtccttcaa
     1441 agtatagtcc agcttcaatt catcccattt gaatctcccc agctggtgga aagggagcac
     1501 gtccacgcgt tccacgttgc ccagaagcgc cgcatggcgg gagagttcct caatttcttc
     1561 cagattgtcc gtaacgccag gtaccagcac atacctcagc cacacgggct tgcggaggga
     1621 ggccaggcgt tccgcaaact ccagtgtggg gcgcagttct ccgcctgtca gggcgcgata
     1681 ccgtttcgga ttccatgcct tgatatccag caggaccagg tccgtatccg ccagcatttc
     1741 ttcgcccgca ttcacgccca gatggccgga ggtgtccagg caggtgtgca ggcccagttt
     1801 ccggcagcct ttcagcacgg caccggcaaa atccggctgg aagaggggat ccccgccgga
     1861 gagggtgact ccgccgcccg ccgcctgcaa aaaatcccgg taacgggcta tttccttgag
     1921 gacatcatct gcgctcctga gctggcccct ccgcacgtag gaggtatccg gattatggca
     1981 gtagcggcaa cgcaggctgc agccggacag gaaaaggacg aagcggatgc cggggccgtc
     2041 cacggtgccg caggattcca cggaatgaac caaaccggtg acggagcttt ctccgtccgg
     2101 atcatggttt tgtggaaaaa cctgcggcat ccgcttgcag tccgggttaa cgggtgtgga
     2161 aagtgcggtt gatgacctcc tgctgctgtt cccgggtcag cttgatgaaa ttcacggcat
     2221 agccggaaac gcggatggta agctgcgggt atttttccgg gtgatccatg gcgtcgagaa
     2281 gcgtttcccg ttccagtacg ttcacattaa tgtggtgacc tgtagcggca aagtaggcgt
     2341 ccagcaggga gacgagcttg acgcggcgtt cacgttcctc cttgcccaag gcctgaggaa
     2401 cgatggagaa ggtgtaggag atgccgtcca gggagtcgtc ataggacagc ttggctacgg
     2461 agagcatgga ggccacggct ccgttcctgt cccttccgtg catggggttg gctccggggg
     2521 cgaacggttc cccggctcgg cggccgtccg gcgtgttgcc cgtctttttg ccgtacacca
     2581 cgttggaggt gatcgtcagg atggactggg tgggcaggga attgcggtaa gtgtggagcc
     2641 tgcgcagttt ttccatgaaa ttgctgacca ggctgcatgc tatgtcgtcc acgcgcgggt
     2701 cattgttccc gtaacagggg aattcccctt ccgtcttgaa gtccacaatc agcccttctt
     2761 cattgcggat ggcttttacg gtggcatact tgatggcgga cagggagtcc gccgccacgg
     2821 aaagcccggc gattcccgta gccatcgtgc gcagaatttc cggatcatgc agcgccattt
     2881 caatgcgttc atagcagtat ttgtcgtgca tgtagtggat gatgttcagc gcgtcaatgt
     2941 aagttttggc gagccagtcc tgcatcttgt cgtacagggc catcacctca tcatagttga
     3001 gaatttcctc cgtgtagcgg ggggagggcg gggccacctg cttgcccttg agttcatcca
     3061 cgccgccgtt cagggcgtag agaaggcact tggcgaggtt ggcgcgcgct ccgaagaact
     3121 gcatctgctt gccgatgcgc atggcggaca cgcagcatgc gatgccgtag tcgtcgcccc
     3181 aatgcgggcg catcaggtcg tcgttctcgt attggacgga ggacgtttca atggaaacct
     3241 tggcgcagaa gcttttgaag gcttccggca ggttccggga ccacagaacg gtcaggttcg
     3301 gttccggagc cggccccagg ttgtacaggg tctgcagcat gcggaaggaa ctgcgggtga
     3361 cgagggtgcg cccgtcctct cccatgccgc cgatggattc cgtcacccag gtggggtctc
     3421 cggagaacag gttattgtaa tcaggcgtgc ggatgaaacg gacgatgcgc agcttcatca
     3481 cgaactggtc gatgatttcc tgaacttcct cttccgtgat gagcctctgt tccaggtcgc
     3541 gggtgaaata aatgtcgaag aaagtggaaa cgcggcccag ggacatggcc gctccgttct
     3601 gttccttcac ggcggccagg tagcccaggt acgtccattg cacggcttcg cgggcgtttg
     3661 cggcggggcg agtgaggtcg cagccgtagg aggcgcccag ctgggcgagt tcttccaggg
     3721 cgcgtatttg ttcgctcatt tcttcgcgca gacggatcag ctcatccgta agggggggat
     3781 gttcacggtt tttgaggtcc ttgcggcgtt cggcaatcag cttatccgtg ccgtacaggg
     3841 ccacccggcg gtagtccccg atgatgcggc cgcggccata ggcgtccgga aggccggtaa
     3901 taatcccggc ggaacgggcc gcccggatat cggaagtgta ggcgtcgaaa acgccttcat
     3961 tgtgcgtctt gcgtatcttt ttgaaaatgt ctgcggtttt ctcgcacatc ttgaacccgt
     4021 aggattccag ggcctgttgg gccatgcgca agccgccaaa gggcatcagg gcccgtttca
     4081 ggggggcgtc cgtctggagg cctacgacca cttccagttc cttgtcaatg tagccgggct
     4141 tgtgggatgt gatggaggat accacttctt catcagcatc cagcacgccg ccgttgtcta
     4201 tttcccgttt cagcaggact ttcagctcgt cccataaacg aagcgtgcgc tgggaggggc
     4261 cggaaagaaa ctcttcattg ccggaatagg gcgtgtagtt atgctggatg aaatcccgta
     4321 cgtcaatgga ctccgtccat gctcccggct tgaatccctg ccattcctgc ggaagggcgg
     4381 tggactcctt caagtctttt actatgctga tcataaatag aatgtagtga agaagactgg
     4441 agaaaaacgt tccgcagccc ctgaaaggct ggaaacggca aaactccgcg ccgcgtaatt
     4501 atatcgtgaa ttccggggga caacaaggct aattctttgc gaagagaacg cgggtgcgct
     4561 ggctttggca tgtttcacct gtctgtccgt tgcggagcgg aaaatgaggg gaacatgcga
     4621 ttgcggaaaa gtgacggaag attatttctc tttactttcc taatctctat tgcagtatct
     4681 caacgcttac cgttatgaaa tcttatgcat gcctttgttt attgctggcg ggagtttttt
     4741 gcgttgcttg tttctccggg gcctgtgctg atgaattgcc gcccacgct
//
