LOCUS       c00145_gnlPro..         4018 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|DAABDJMK_145.
ACCESSION   c00145_gnlPro..
VERSION     c00145_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 11:25:14
            Original ID  :: gnl|Prokka|DAABDJMK_145
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4018
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4018
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[71:3047]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      72..3047
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4018
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4018
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             72..2312
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg145_1"
                     /sec_met_domain="Gly_radical (E-value: 1.6e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2.3e-282, bitscore:
                     925.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRRVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             2313..3047
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg145_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.1e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             3246..3824
                     /locus_tag="ctg145_3"
                     /transl_table=11
                     /translation="MKTPQEIAEMISKRFYPLPDADLDSLASIIESSKAAKGELLLDNG
                     QISKYLYYVESGLLRQFYYKNKRDITEHFSCEGNIVICIASTFQREPTRLLIEALEPTV
                     YHRISFSEFSDLLKTSLAINRLYQKILEWALMLSQEKADSWRFESARKRYLRFQQEYPE
                     AAKRASVNHIASYLLMTPESLSRVRAGVL"
     CDS             3932..4018
                     /locus_tag="ctg145_4"
                     /transl_table=11
                     /translation="MKIEKITGREILDSRGNPTVEVDILLESG"
ORIGIN
        1 tgtgacagaa atatgacgat aattctccta cctttgcggc ggtaaaaatg tggtattaga
       61 taagattgtt tatgaaattt gagaatgagt gctgggatca ctttaaaggt gagacctgga
      121 aaagagagat caatgtaaga gattttatcc agagtaacta tacgccttat gaaggggatg
      181 attccttttt agtcgcttct tccgagaaga cccgtagggt ctggaacaag ctgaccgaga
      241 tgtttaaggt cgagagggaa aagggagttt atgacgccga gacaaaatta cctcaaggga
      301 tcgatgtata tggcccgggc tatattgata aggagaatga ggtgatcgtt gggttacaaa
      361 ccgatgctcc gctgaaacgt ggtatcttcc cgaagggcgg tatccgtatg gttgagaata
      421 gcttggaagc ttatggttat catttggacc cgatgacgaa agagatcttc acgaagtacc
      481 gtaaaactca taacgaaggc gttttctccg catatacgga agagatgatt gccgcacgtc
      541 gttcggctat tatcaccggt ctgcccgacg cgtatggtag aggacgtatt attggtgact
      601 atcgtcgtgt cgctttatat ggtacggcaa tcttgatcga ggagaaaaag cgtttcttga
      661 atcgtttgga tatccaagag atcacggaag agatcatcca gagccgtgag gagatttccg
      721 aacagatcaa ggctttgaaa gcgttcgaga aaatgtgcgc tagctatggc tttgatgtga
      781 ctcgtccggc acaaaacgct cgtgaggcag ttcagtttgt gtacttagct tatctggcgg
      841 cggtaaaaga ccaagatggc gcggcgatgt ctatcggacg tacttctacg tttttggata
      901 tttatatcga gaaagacatc cgtgagggta agttgaccga agaggaggcg caagaattgg
      961 tagaccagct gatcatcaag ttgcgtatcg ttcgtttctt acgtacgccg gaatacaatg
     1021 atttgttctc cggtgatccg gtttgggtaa cagagtcctt gggcggccaa ggagtggatg
     1081 gccgttcttt ggtaacaaga acttcctatc gttatctcca tacattatat aatctaggtc
     1141 cggcaccaga acccaatttg acggttctgt ggtttaagaa cgctcccgag aactggaaac
     1201 gtttctgtgc caaggtctct atcgatacat cggctatcca atatgagaac gacgatttga
     1261 tgcgtccgga ttatggagat gattatggta tcgcttgttg cgtttctcct atgaagatcg
     1321 gtaagcagat gcagttcttc ggagcccgtg cgaatttggc gaaatgttta ttatatgcga
     1381 tcaacggagg acgggatgag cgcagcggcg tacaggtagc cccgatgttc gagccggttc
     1441 gcggcgagta tctggaatac gatgaggtga tggctaagta tgagcaaatg atgcgctggt
     1501 tggcgaaagt gtatgtgaac gccttgaaga taatccatta tatgcatgat aaatacgcat
     1561 atgaggcatt cgagatgtcg cttcacgatg gagatgtaga gcgtatccgt gcgacaggta
     1621 tcgccggtct ttctatcgtt gccgattcgt tggctgctat ccgtgatacg aaagtacgtg
     1681 tgatccgtga cgagcgtggc ttggcggttg atttcgaacg tgagggcgaa tatgttcctt
     1741 tcggtaataa cgacgatcgt acggatagta tcgctgtaga gatcaccgag aagttcatgg
     1801 aatatttgcg tcagcacgag acctatcgcc atgcgaaacc tacgcagtcc atccttacga
     1861 tcacctctaa tgtggtatat ggtaagaaga ctggtacgac accggatggc cgtatcggtg
     1921 gaactccttt cgctccgggc gcgaacccta tgaacggtcg tgatacgaaa ggtgctatcg
     1981 ctgcgctagc ttcggtagcg aagcttccat tccagcatgc gcacgacggt atctcttata
     2041 ctttcgctgt ttctccggct acgttgggaa aggagcgtga agtgcagatc aataatctgg
     2101 ttagcttatt ggatggctat tttactccgg atggaggtca gcatctgaat gttaacgtat
     2161 ttgataagga tttgttgatc gacgctatgg aacatcctga gaaatatccg caacttacga
     2221 tccgtgtatc cggatatgcg gtaaacttcg tgaaactgac tcgtgaacaa cagttggatg
     2281 ttatttcaag aaccattaac cattcactgt aagtgataaa aggtaagata cattcattag
     2341 agagtttcgg gaccgtagat ggtcccggaa ttcgctttgt cgtttttatg cagggctgcc
     2401 cgttgcgttg cctgtattgc cataacccgg atacttggaa cccgaaaggg aaggtgaaat
     2461 atcagatgac tcccggcgag ctattgacgg aagtcttacg gtataagagt tttatcgccc
     2521 gtgggggggt gactgttacc ggtggggaac ctttattgca acctgagttt ctgaaagagt
     2581 ttttccgttt gtgccaagag caaggtcttc acacagcttt ggacacatct ggtttcgtat
     2641 gtacctcaaa agcttgggaa gtcttggatt atgcggactt agtgttattg gacatcaaaa
     2701 ccttaaaccc ggatttacat ccgttactgg cgggtgtcaa gcaggataat acgcttctgt
     2761 tcttggatga attggagaga cgtggcatag atacttggat tcgccatgtt attgtccccg
     2821 gatatacgga taatgacgag tggctggagg cgttagctcg gtatgtcagt tcatataaag
     2881 tggttcgcaa agtggaatta ttgccttatc atacgatggg aacctataaa tatgagcaat
     2941 taggtctcga ttatccgtta aagggggtgg aacccttatc aaaagaacgt ttagataatg
     3001 cgaaagcgat attctctcgc tacaaaccag aaaataacaa ggcctgatct ctcgatcaag
     3061 ccttgcgatt atgcaacagc taatcaggga aaacaatcta atccgattag cttttaaacg
     3121 atagtaataa ttttttgcct agactaacct tgactattta atatgtgttt gttttgttat
     3181 atgcaacaaa tatctatttt tgtaaggaga taaaaaatga tatagatcag aaaatgatga
     3241 atagtatgaa gacaccacaa gaaatagccg aaatgatatc caaacgtttt tatccattac
     3301 ccgatgcgga tttggattct ctggcctcga tcatagaatc aagtaaggct gcgaaaggag
     3361 aattattgtt ggataacgga caaatatcta aatatttata ttatgtggaa tcgggattat
     3421 tacgccagtt ttactataag aataaacgtg atattaccga gcatttctcc tgtgagggaa
     3481 atatcgttat ttgtatagcc agcacattcc aaagagaacc gacgcgttta ttaatcgagg
     3541 cattggaacc gacggtttat caccggatct ctttttcgga atttagcgat ttattgaaaa
     3601 catctttagc tattaataga ttgtaccaga aaatactgga gtgggctttg atgctttcgc
     3661 aagagaaagc ggactcttgg cgttttgagt cggcaagaaa acgttatctg cgttttcaac
     3721 aggaatatcc ggaagcagcg aaacgtgctt ctgtcaatca tatcgcctcg tatttattga
     3781 tgacacccga atctttgagc cgggtgcggg cgggagtgtt gtagtctcta cctacattca
     3841 ggtattattg aggtgaaaat agcaatttgt ggtgatttca cggttcaagt aatccatcta
     3901 tatttgcgtc agtttaaaaa acgtagaagc aatgaaaata gaaaagatta caggacgtga
     3961 gatcctagat tcaagaggta acccgacagt agaggttgat attttactag agtccggc
//
