LOCUS       c00416_gnlPro..         3639 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OCIBECHM_416.
ACCESSION   c00416_gnlPro..
VERSION     c00416_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 10:04:33
            Original ID  :: gnl|Prokka|OCIBECHM_416
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3639
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3639
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[126:3102]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      127..3102
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3639
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3639
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(127..861)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg416_1"
                     /sec_met_domain="Radical_SAM (E-value: 3.8e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             complement(862..3102)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg416_2"
                     /sec_met_domain="Gly_radical (E-value: 1.2e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.7e-282, bitscore:
                     925.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRRVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             complement(3273..3638)
                     /locus_tag="ctg416_3"
                     /transl_table=11
                     /translation="LHDALPISECWVLLDRNRLKQVWMNFLTNAVKCTKSGYIKMGYGI
                     ERKGLRIYVEDTGIGIPDDLHDKVFTRFEKLNEFSQGTGLGLTISRAIVEAAGGEVGFT
                     SKLGVGSTFWAWLPLRP"
ORIGIN
        1 aacaaacaca tattaaatag tcaaggttag tctaggcaaa aaattattac tatcgtttaa
       61 aagctaatcg gattagattg ttttccctga ttagctgttg cataatcgca aggcttgatc
      121 gagagatcag gccttgttat tttctggttt gtagcgagag aatatcgctt tcgcattatc
      181 taaacgttct tttgataagg gttccacccc ctttaacgga taatcgagac ctaattgctc
      241 atatttatag gttcccatcg tatgataagg caataattcc actttgcgaa ccactttata
      301 tgaactgaca taccgagcta acgcctccag ccactcgtca ttatccgtat atccggggac
      361 aataacatgg cgaatccaag tatctatgcc acgtctctcc aattcatcca agaacagaag
      421 cgtattatcc tgcttgacac ccgccagtaa cggatgtaaa tccgggttta aggttttgat
      481 gtccaataac actaagtccg cataatccaa gacttcccaa gcttttgagg tacatacgaa
      541 accagatgtg tccaaagctg tgtgaagacc ttgctcttgg cacaaacgga aaaactcttt
      601 cagaaactca ggttgcaata aaggttcccc accggtaaca gtcacccccc cacgggcgat
      661 aaaactctta taccgtaaga cttccgtcaa tagctcgccg ggagtcatct gatatttcac
      721 cttccctttc gggttccaag tatccgggtt atggcaatac aggcaacgca acgggcagcc
      781 ctgcataaaa acgacaaagc gaattccggg accatctacg gtcccgaaac tctctaatga
      841 atgtatctta ccttttatca cttacagtga atggttaatg gttcttgaaa taacatccaa
      901 ctgttgttca cgagtcagtt tcacgaagtt taccgcatat ccggatacac ggatcgtaag
      961 ttgcggatat ttctcaggat gttccatagc gtcgatcaac aaatccttat caaatacgtt
     1021 aacattcaga tgctgacctc catccggagt aaaatagcca tccaataagc taaccagatt
     1081 attgatctgc acttcacgct cctttcccaa cgtagccgga gaaacagcga aagtataaga
     1141 gataccgtcg tgcgcatgct ggaatggaag cttcgctacc gaagctagcg cagcgatagc
     1201 acctttcgta tcacgaccgt tcatagggtt cgcgcccgga gcgaaaggag ttccaccgat
     1261 acggccatcc ggtgtcgtac cagtcttctt accatatacc acattagagg tgatcgtaag
     1321 gatggactgc gtaggtttcg catggcgata ggtctcgtgc tgacgcaaat attccatgaa
     1381 cttctcggtg atctctacag cgatactatc cgtacgatcg tcgttattac cgaaaggaac
     1441 atattcgccc tcacgttcga aatcaaccgc caagccacgc tcgtcacgga tcacacgtac
     1501 tttcgtatca cggatagcag ccaacgaatc ggcaacgata gaaagaccgg cgatacctgt
     1561 cgcacggata cgctctacat ctccatcgtg aagcgacatc tcgaatgcct catatgcgta
     1621 tttatcatgc atataatgga ttatcttcaa ggcgttcaca tacactttcg ccaaccagcg
     1681 catcatttgc tcatacttag ccatcacctc atcgtattcc agatactcgc cgcgaaccgg
     1741 ctcgaacatc ggggctacct gtacgccgct gcgctcatcc cgtcctccgt tgatcgcata
     1801 taataaacat ttcgccaaat tcgcacgggc tccgaagaac tgcatctgct taccgatctt
     1861 cataggagaa acgcaacaag cgataccata atcatctcca taatccggac gcatcaaatc
     1921 gtcgttctca tattggatag ccgatgtatc gatagagacc ttggcacaga aacgtttcca
     1981 gttctcggga gcgttcttaa accacagaac cgtcaaattg ggttctggtg ccggacctag
     2041 attatataat gtatggagat aacgatagga agttcttgtt accaaagaac ggccatccac
     2101 tccttggccg cccaaggact ctgttaccca aaccggatca ccggagaaca aatcattgta
     2161 ttccggcgta cgtaagaaac gaacgatacg caacttgatg atcagctggt ctaccaattc
     2221 ttgcgcctcc tcttcggtca acttaccctc acggatgtct ttctcgatat aaatatccaa
     2281 aaacgtagaa gtacgtccga tagacatcgc cgcgccatct tggtctttta ccgccgccag
     2341 ataagctaag tacacaaact gaactgcctc acgagcgttt tgtgccggac gagtcacatc
     2401 aaagccatag ctagcgcaca ttttctcgaa cgctttcaaa gccttgatct gttcggaaat
     2461 ctcctcacgg ctctggatga tctcttccgt gatctcttgg atatccaaac gattcaagaa
     2521 acgctttttc tcctcgatca agattgccgt accatataaa gcgacacgac gatagtcacc
     2581 aataatacgt cctctaccat acgcgtcggg cagaccggtg ataatagccg aacgacgtgc
     2641 ggcaatcatc tcttccgtat atgcggagaa aacgccttcg ttatgagttt tacggtactt
     2701 cgtgaagatc tctttcgtca tcgggtccaa atgataacca taagcttcca agctattctc
     2761 aaccatacgg ataccgccct tcgggaagat accacgtttc agcggagcat cggtttgtaa
     2821 cccaacgatc acctcattct ccttatcaat atagcccggg ccatatacat cgatcccttg
     2881 aggtaatttt gtctcggcgt cataaactcc cttttccctc tcgaccttaa acatctcggt
     2941 cagcttgttc cagaccctac gggtcttctc ggaagaagcg actaaaaagg aatcatcccc
     3001 ttcataaggc gtatagttac tctggataaa atctcttaca ttgatctctc ttttccaggt
     3061 ctcaccttta aagtgatccc agcactcatt ctcaaatttc ataaacaatc ttatctaata
     3121 ccacattttt accgccgcaa aggtaggaga attatcgtca tatttctgtc acatatgcta
     3181 caccagttct aaaatcccta taaattgcga taaaaaagct tctcatcctt cactatttta
     3241 aaaggacatt accaataaat ggggagtcag acctaagggc gaagaggcaa ccatgcccaa
     3301 aaagtagatc cgacaccaag ttttgaagta aagcccactt ctccccctgc cgcttctacg
     3361 atagctctgg agatcgtcaa gccaagtccg gtaccctgcg agaactcatt caatttctca
     3421 aaacgggtaa atactttgtc atgcaaatca tccggtatac cgattcccgt atcctcaaca
     3481 tagatacgaa gacctttcct ttcaatgcca tatcccattt taatatatcc ggatttggtg
     3541 cattttaccg cattagtgag gaaattcatc catacttgct taagccggtt tctatccaga
     3601 agtacccagc actccgagat cggaagagcg tcgtgtagg
//
