LOCUS       c00186_gnlPro..         7162 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KJAHFGDN_186.
ACCESSION   c00186_gnlPro..
VERSION     c00186_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 16:57:33
            Original ID  :: gnl|Prokka|KJAHFGDN_186
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7162
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7162
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[12:4548]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      13..4548
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..7162
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7162
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(13..1173)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_1"
                     /sec_met_domain="OAD_beta (E-value: 3.2e-154, bitscore:
                     501.7, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQESFLQFLGENIQIFLSYTGFANATPGHIVMLLVGLLFIFLAIK
                     YEFEPMLLIPIGFGILIGNIPFKDAGLQLGIYEEGSVLNILYQGVKQGWYPPLIFLGIG
                     AMTDFSALISNPKLMLVGAAAQFGIFGAYIIALQMGFDPSQAGAIGIIGGADGPTAIFL
                     SSKLAPNLMGAIAVSAYSYMALVPIIQPPFMRLLTTKKERVIRMKPPRAVSQTEKIVFP
                     IVGLLLTAFLVPSGLPLLGMLFFGNLIKESGVTRRLAETARGPLIDVITILLGITVGAS
                     TQATQFLTLNSIKIFGLGALSFVIATCAGVLFVKFFNLFLKEGNKINPLIGNSGVSAVP
                     DAARISQNVGLEYDPSNYLLMHAMGPNVAGVIGSAVAAGILLGFLG"
     CDS             complement(1178..1609)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 63.7; E-value:
                     3.5e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_2"
                     /sec_met_domain="Biotin_lipoyl (E-value: 3.4e-21, bitscore:
                     63.7, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKSFKYTINGNTYKVHINSVVDDVAEVEVNGTPYNVKMEKPAKKQ
                     MVTLKRPAQAPTNASGEPVITRQAAPSVAGAVKSPLPGVILSVDCKVGDTVKRGQKILV
                     LEAMKMENNINADRDGKIVEIKVNKGDSVLEGADLVVIG"
     CDS             complement(1633..2526)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_3"
                     /sec_met_domain="OAD_gamma (E-value: 8e-14, bitscore: 40.9,
                     seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINKKIGVLLLLMVLFSMGAKAQLTTSVKLNEVLVVNEDNFVDDY
                     GKRHPWFELFNNSAGTVDLRGCFLTNDKSNPRKYMIPKGDVLTKVPPRQHLLFWADDQP
                     TKGTFHVNFTFDPNKDNYLALYDSDGTTLIDEVTIPAGQKADVSYGLDLDGVGNWSILD
                     KVTPSTNNLTLDSNAKLENFQRNDPWGIGMTITAMAVVFLGLIILYLVFKQIGNAAIKA
                     SKRNAQKAAGTGAAVSANIGQESGEIFAAIATALYEMSDDNHDIENTVLTIHKVQRSYS
                     PWSSKLYSLRQTPNKK"
     CDS             complement(2548..4101)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Btheta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_4"
                     /sec_met_domain="CT_Btheta (E-value: 5e-293, bitscore:
                     960.5, seeds: 72, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSNQLEKVKELIALREQARLGGGEKRIESQHKKGKYTARERIAML
                     LDEGSFEEFDMFVQHRCTNFGIEKTKYLGDGIVTGYGTIDGRLVYIFAQDFTVFGGALS
                     EALAMKICKVMDQAMKMGAPVIGLNDSGGARIQEGVNALAGYAEIFQRNILASGVVPQI
                     SGIFGPCAGGAVYSPALTDFNIMTRGTSYMFLTGPKVVKTVTGEDVTQEQLGGASVHTT
                     KSGVAHFAVDTEEDGLQLIRKLLSFLPQNNLEETPLVECKDPIDRLDDVLNDIIPDNAN
                     QSYDMYEVIGTIVDNNEFLEVHADYAKNIIVGFARFNGQSVGIVANQPKVMAGCLDSNA
                     SRKAGRFVRFCDAFNIPLVTLVDVPGFLPGTGQEYNGVILHGAKLLYAYGEATVPKITV
                     TLRKSYGGAYCVMSSKHLRGDMNYAWPTAEIAVMGPSGAVEVIFAKEVAAAEDPKACAA
                     EKEAEYKKAFANPYNAAQYGYIDDVIEPRNTRFRIIRALQQLQTKKLSNPAKKHDNLPL
                     "
     CDS             complement(4138..4548)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg186_5"
                     /sec_met_domain="Glyoxalase_4 (E-value: 1.3e-27, bitscore:
                     85.0, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELTHIEHLGIAVKSIEACLPYYEGVLGLKCYNIEVVADQKVKTA
                     FFKVGQTKIELLEPTSEDSTIAKFIEKKGEGIHHIAFAVPDVQAALNEAEEKGVKLIDK
                     APRGGAEGLNIAFLHPKSTCSVLTELCMPGNK"
     CDS             complement(4677..7088)
                     /locus_tag="ctg186_6"
                     /transl_table=11
                     /translation="MKIRAIAALLFILISVSAFGEGRIKITGYVRDADGNPLELVNVRV
                     KNTLIGSMTNEKGYYSFSISPGDSISIIYSCLGYNKAERIIPSAQADMRLNVQMNNTSF
                     DLGEVSVTAIRKQTTTMESLNADKIKLLPDPSGGSIESLVVTFAGVSSNNELSSQYSVR
                     GGSYDENIVYVNGIEVFRPLLIRSGQQEGLSFINPDLTEAVNFAAGGFEARYGDKMSSV
                     LDITYKKPKIFEGSASASLLGANAYVGSSIGKFTQVTGFRFKSGRSILGTMDTDAEYNP
                     KFIDLQTYITYQLAPKWEINFLGNLANNNYKFTPYSRETSFGTAEHPKNFKVYFDGRER
                     DRFQTLFGALTLKHNPNENTELGLQASAFKSKEEEGYDIAGEYWLSENGAENPDDDASA
                     AMSVGRYHEHARNRLNSTVMNVGHYGMARLLNNTLKWGATVQMEKINDKISEWEKRDSS
                     GYSLPQTGNNVSVYSNLFSDNQIESTRFSAYAQDAFKFRTKQGLFTLVAGVRGSYWTYN
                     KEFLFSPRASLGFIPNFDQDLTLRFATGLYYQSPFYKELRRVDKDKNGNNITVLNKDLK
                     SQRSIHFILGGDYTFRAVDRNFKVTAEMYYKKLDNLNPYTVDNVKIRYYGENCAKGYAM
                     GLDVKFFGEFVPGTDSWISFSLMKAQQTIRETTTVPMANSQGYNISLFFQDYFPGYKRV
                     KLNLKGVLSGGLPQTIPGKGYEAGYFRTPAYKRVDIGLSYQLAGGTDAIMDRSFFRHLK
                     NIWLGLDVFNILDIKNVSSYYWITDVYNVQYAVPNYLTGRQLNVRLIVDF"
ORIGIN
        1 atagttgttt tattaaccaa ggaatcctag caagataccg gcagctacag cggaaccgat
       61 tacacctgct acgttcggac ccatagcgtg catcaataag tagttacttg gatcgtattc
      121 caaacctacg ttctgggaaa tacgggcagc gtcaggtacg gcggatacac cggagttacc
      181 gatcaacgga ttgatcttgt tgccttcttt caggaacagg ttaaagaact taacgaacaa
      241 tacaccggca caagtagcga taacgaatga caacgcaccc aaaccgaaaa tcttgataga
      301 gttcaatgtt aagaactggg tagcttgtgt agaagcacct accgtgatgc ctaataagat
      361 cgtgataaca tcgatcagcg ggccgcgggc tgtctctgcc aaacggcgag taacaccact
      421 ttccttgatc aagttaccga agaacaacat tcctaacaat ggcaaaccag acggtaccaa
      481 gaaggctgtt aataacaagc caacgatcgg gaaaacgatt ttctccgtct gtgaaacggc
      541 tcttggaggt ttcatacgta tcacacgttc tttcttcgtt gtaagcaatc tcataaacgg
      601 tggctggata ataggaacca atgccatata tgagtatgcg gatacagcga tagcacccat
      661 caagttagga gctaacttgg atgacaagaa gattgcggtt ggaccatcag ctcctccgat
      721 aataccgata gcaccggctt ggctcgggtc aaatcccatt tgcaatgcaa tgatataagc
      781 tccgaaaata ccaaattggg cggcagctcc gactagcatt aacttaggat tggatatcaa
      841 agcggagaag tctgtcatgg caccgatacc gaggaatatc aaggggggat accaaccttg
      901 tttcactcct tggtataaaa tgttcaatac agaaccctct tcgtatatac ctaattgtaa
      961 tccggcatcc ttgaaaggga tatttccgat caagataccg aaaccaatag ggatcagaag
     1021 cattggctcg aattcatatt taatcgctag gaatatgaac agcaaaccta ccaatagcat
     1081 cacgatatgc cctggagtcg cgttcgcaaa tcctgtatag gagagaaata tttggatatt
     1141 ttctcctagg aattgtaaga aactttcttg catatcatta accgattaca acgagatctg
     1201 caccttccaa tacagagtcg cctttgttta ctttaatttc tacaatttta ccgtcacgat
     1261 cggcattgat gttgttttcc atcttcatgg cttccaatac caagatcttt tggcctctct
     1321 taactgtatc gccgacttta cagtctacac ttaaaattac acctggcagc ggagatttaa
     1381 cagcgccggc tacacttgga gctgcttgac gggtgataac aggttcaccg ctggcattgg
     1441 tcggagcttg agccggacgt ttcaatgtaa ccatttgttt tttcgcaggt ttctccattt
     1501 ttacgttata aggggttccg tttacctcta cctctgccac atcatctaca actgagttga
     1561 tatgtacttt atatgtgtta ccgttaatgg tatatttaaa acttttcatt cgtcaattgg
     1621 attttaggat gattactttt tatttggagt ctgacgcaga gaataaagct tagaactcca
     1681 tggtgaatag ctgcgttgaa ccttgtggat agtcaatacc gtgttctcga tatcgtggtt
     1741 gtcatcgctc atctcataaa gagcggtagc gatagcggca aaaatttcac ctgattcttg
     1801 accgatattg gctgatacgg cagcacctgt tcctgcggct ttttgagcgt tacgcttgct
     1861 agctttgatc gctgcgttac cgatctgctt gaatactaga tacaaaatga ttaagccaag
     1921 gaatacgact gccatggctg ttatggtcat accaataccc catgggtcgt tccgttggaa
     1981 gttctcaagt ttcgcattcg aatctaacgt cagattattg gtgctaggag tcactttgtc
     2041 aaggatactc caattaccta ctccatcaag atccagaccg taactaacat ctgctttttg
     2101 cccggcaggg attgtcacct cgtcgatcaa agttgtacca tctgaatcat ataaggctag
     2161 ataattatct ttgtttggat cgaatgtgaa gtttacatga aatgtccctt ttgttggctg
     2221 atcatctgcc caaaaaagaa gatgctggcg gggaggaact ttggtaagta catctccctt
     2281 cgggatcata tattttctgg gattactttt atcgttcgtt aagaagcatc ctcgtaaatc
     2341 caccgtacct gctgagttat taaacaactc aaaccatgga tggcgttttc catagtcatc
     2401 aacaaagtta tcttcattga caactagcac ttcatttaat ttaacggagg tagtcaactg
     2461 tgccttggct cccatactga agagaaccat taacagtagc aataccccta ttttcttatt
     2521 tatcatattc atctcctgtt ttacggatta tagaggcagg ttatcatgtt tcttagcagg
     2581 attgcttaac ttcttagtct gcaattgctg caaagcacga ataatacgga aacgagtgtt
     2641 acgcggttcg ataacatcgt cgatataacc gtattgagct gcgttataag gattagcgaa
     2701 tgctttctta tattcagctt ctttctctgc tgcgcatgct ttcggatctt cagcggctgc
     2761 tacttccttg gcgaagataa cctctacggc accgctcggg cccataacgg cgatctcggc
     2821 tgtcggccat gcgtaattca tatcaccacg taaatgttta gaactcatca cgcaataagc
     2881 tccaccgtaa gacttacgca atgtaacggt aatcttagga acggtagcct caccataagc
     2941 gtaaagcaat ttagcgccat gaaggattac accgttgtat tcctgacctg tacccggaag
     3001 gaatcccgga acgtcaacca atgttaccaa aggaatattg aaagcgtcgc agaaacgaac
     3061 gaagcgacct gctttacgag atgcgttgct atccaagcat cctgccataa ccttcggctg
     3121 gttagctacg ataccgacgg attgtccgtt gaaacgagcg aaacctacga tgatattctt
     3181 agcgtagtcc gcatggacct ctaggaactc gttgttatcg acgatcgtac cgataacctc
     3241 atacatatcg taagattggt tggcgttatc gggaatgata tcgttcaata catcatccag
     3301 acgatcgatc gggtctttgc actcaactaa cggcgtttcc tctaagttgt tctgaggtaa
     3361 gaaactgagc aatttacgaa tcaattgcaa accatcttct tctgtatcga cagcgaaatg
     3421 ggctacaccc gatttggtag tgtgtacgct ggctccaccc aactgctctt gggttacatc
     3481 ctcacctgtt acggttttta cgactttcgg accagtcaag aacatatagc tggtaccacg
     3541 tgtcatgatg ttgaagtctg tcaaagccgg agaataaacg gctccaccgg cgcaagggcc
     3601 gaagataccg gaaatctggg gaacaacacc ggaagccaag atgttacgtt ggaaaatctc
     3661 tgcgtatcct gccaaggcat ttactccttc ttggatacga gctccaccgg agtcgttcaa
     3721 accgataacg ggagcgccca tcttcatggc ttgatccatt actttacaga tcttcatagc
     3781 caaagcctca gacaaggctc caccgaatac ggtgaaatct tgagcgaaga tatataccaa
     3841 acgtccgtcg atcgtaccat aacctgttac gataccatcg cctaaatatt tagttttctc
     3901 gataccgaag tttgtacaac ggtgttgaac aaacatgtcg aattcctcga aactaccttc
     3961 gtcaagcaac atggcgatac gctcacgagc ggtatatttg ccttttttgt gctgtgattc
     4021 gattcttttc tctcctccac ccaagcgagc ttgctcacga agtgcgatga gctcttttac
     4081 tttttcaagc tggttactca ttgtattttg atttgttgtt agaaatgaac ggattaatta
     4141 tttgttgccc ggcatgcaaa gttctgtcaa tacgctgcaa gttgatttcg ggtgaaggaa
     4201 agcgatgttc aagccttccg caccaccacg gggagcttta tcgattaatt taacgccttt
     4261 ttcctcagcc tcgttcaaag ctgcctgtac atcaggtacc gcgaatgcga tatggtggat
     4321 accttcacct tttttctcga taaatttcgc gatcgtgcta tcttcgctgg tcggctctag
     4381 taattcaatc ttagtctgtc ctaccttgaa aaatgcggtt tttacttttt ggtctgctac
     4441 tacttcgata ttgtagcatt ttaatccaag aacgccttca taatagggta aacaagcctc
     4501 gatactcttc actgcgatac ctaaatgttc gatatgtgta agttccatgt caataataat
     4561 ttaaagattt agtaaatgtt tgtttctatt gtgtgcaaat gtacactact ttttatgaat
     4621 gaggaaataa ttttttgttt ttatctccca gtaaatgtgt ttaaagatac attttattaa
     4681 aaatcgacaa tcagacgaac gttcaattga cgtccggtca gatagtttgg aactgcgtat
     4741 tggacattat atacatctgt tatccaatag taggaactaa cgtttttgat gtctaatatg
     4801 ttaaacacat ccagacctag ccaaatgttt ttcaaatggc ggaagaagct tctgtccatg
     4861 atagcgtccg tgcctcctgc caattgatag gagagtccga tatctacacg tttgtaggcg
     4921 ggcgtacgga aatagccggc ttcgtatcct ttgcccggga ttgtctgggg aagtcctccg
     4981 gatagaactc ctttcagatt tagcttgact cgtttatagc ccgggaaata atcttggaag
     5041 aagagtgaga tgttgtagcc ttggctattg gccataggaa cggtcgttgt ttcccggatg
     5101 gtttgctggg ctttcatcaa ggagaagctg atccatgagt cggtacccgg tacgaattct
     5161 ccgaagaatt tgacatcgag tcccatggcg taacctttgg cgcagttctc gccgtaatag
     5221 cggatcttga cgttatctac ggtgtaggga ttcagattgt ccagcttttt atagtacatc
     5281 tcggcggtca ccttgaaatt ccggtctacg gcacggaacg tataatctcc tcccaatatg
     5341 aaatggatgg agcgttgtga ctttagatct ttgttcaata ccgtgatatt attgccgttc
     5401 ttatctttgt ctactctacg taattccttg tagaagggcg attgataata cagtccagtg
     5461 gcgaaacgga gcgtcaagtc ttggtcgaag ttcgggataa agcccaatga ggcacgggga
     5521 ctgaaaagga actccttatt ataagtccaa taactacctc taactcctgc gactaacgtg
     5581 aacagacctt gcttggtcct gaatttgaac gcgtcttgcg cataagcgga aaagcgtgtg
     5641 ctttctatct gattatcaga aaagaggttc gaatatacac ttacgttatt accggtttgg
     5701 ggcaaagaat aacctgagga atcccttttt tcccattcac tgatcttatc attgattttc
     5761 tccatttgta ccgtggctcc ccatttcaac gtattgttta aaaggcgggc catcccatag
     5821 tgcccgacat tcatgacggt ggagttcaaa cggtttcgtg cgtgctcgtg ataacggcct
     5881 acggacatgg cggctgacgc gtcgtcatcc ggattttccg ctccgttctc gctcagccaa
     5941 tattctccgg cgatatcgta tccctcttct tccttgcttt tgaaagcgga ggcttgaagt
     6001 cccaactctg tgttctcatt ggggttgtgt ttcaatgtaa gcgcaccgaa taaggtttgg
     6061 aaacggtctc tttcccttcc gtcgaaatag actttgaagt tcttgggatg ctcggctgta
     6121 ccgaaagagg tttcccggct ataaggggtg aatttataat tattgttagc gaggtttccc
     6181 aagaaattga tttcccattt aggggccaat tggtacgtga tgtaagtttg cagatcgata
     6241 aatttagggt tgtattctgc gtctgtatcc atggtaccca aaatagaccg accgctttta
     6301 aagcggaaac ccgttacttg ggtaaattta ccgatagaac tgcctacata agcattcgcg
     6361 cccaacaagc tcgcagaagc tgagccttcg aatatcttag gctttttata ggtaatatcc
     6421 agcacggaac tcatcttgtc tccgtaacga gcctcgaagc caccggcggc gaaattgacc
     6481 gcctccgtca ggtccggatt gataaagcta aggccctctt gttgtcccga gcgaatcaga
     6541 agcggacgga atacctcgat cccattcaca taaacgatat tttcatcata actacctcca
     6601 cgtacggaat actgggaact taactcgtta ttggacgaga ctccggcaaa cgtaacgacc
     6661 aagctctcta tactacctcc ggagggatcg ggcaataact tgattttgtc cgcatttagg
     6721 gactccatgg tcgtggtctg cttgcggatg gcagtaacag atacttcccc taaatcaaat
     6781 gaggtattgt tcatctgtac gttcaggcgc atatcggctt gtgccgaagg aatgatacgt
     6841 tcagctttat tatatcccag acaagagtaa ataatggaga tagaatcccc cggcgatatg
     6901 gaaaaggaat aatatccctt ctcgttggtc atggagccga tcaacgtgtt cttaacccgg
     6961 acattcacta attccagagg gttaccgtcg gcgtcacgga cataaccagt gatttttatg
     7021 cggccttcgc caaaggcact cacggaaata agtataaata gcagcgcggc tatcgcacga
     7081 atcttcataa tctattgttc aattataaaa atactaacga gaaaaaagaa cgatacgtat
     7141 atatgggtgc aaaaaattat at
//
