LOCUS       c00189_gnlPro..         6933 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KJAHFGDN_189.
ACCESSION   c00189_gnlPro..
VERSION     c00189_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 16:57:33
            Original ID  :: gnl|Prokka|KJAHFGDN_189
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6933
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6933
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[938:3914]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      939..3914
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..6933
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6933
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(162..740)
                     /locus_tag="ctg189_1"
                     /transl_table=11
                     /translation="MKTPQEIAEMISKRFYPLPDADLDSLASIIESSKAAKGELLLDNG
                     QISKYLYYVESGLLRQFYYKNKRDITEHFSCEGNIVICIASTFQREPTRLLIEALEPTV
                     YHRIFFSEFSDLLKTSLAINRLYQKILEWALMLSQEKADSWRFESARKRYLRFQQEYPE
                     AAKRASVNHIASYLLMTPESLSRVRAGVL"
     CDS             complement(939..1673)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg189_2"
                     /sec_met_domain="Radical_SAM (E-value: 6.4e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             complement(1674..3914)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg189_3"
                     /sec_met_domain="Gly_radical (E-value: 1.9e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.5e-282, bitscore:
                     926.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             complement(4085..6388)
                     /locus_tag="ctg189_4"
                     /transl_table=11
                     /translation="MEQCEENSQENDDVIIEKEISKLIEEQRLFTESIYACLPVGIEIY
                     DAHGVLRSINDHALRMYGVDDRTSVVNIVNLYKSPFVDSELLARIQSGEDISLEFEYDF
                     DRVNKDAYYSSHNKDTMIYGVKVIPIWSRKKHLIGHILLANDMTSVKDVEFRTEESKKN
                     LEMAMKAANMASWVYDVNKKVFNPLYGLTVAKKNMPMEAVLDILHPQDRAPLIRLFSQL
                     IGNEIEQGQMTLRFYNEQEEMYRYYESRMRLSTEHRGKLQIIGTQMDITERLQMAKKAQ
                     DLIAKRDLAMKVSNIVHWDFDVNSQKFESYNDPINDYVSDRLLTVSEYMDVIYPEDRSV
                     FYDAMQSLIAGKNVTINFTCRIQTKYDETWQYCDFMGVPFDQDENGDIIRYTGFRQNIP
                     KIQQLNRELKERNYKIELSFKTVGMSYWDFDVKSLKFSAFNDPINDFYSEKTITPEDYL
                     NATHPDDVVFVRKYMEHMFRGIDKDFNVKYRSKTKWDDEWQTLLVTGIQVERDKEGHVT
                     RYTGITINNTKWEKMIQQLKELKEKAELSDRLKSAFLANMSHEIRTPLNAIVGFSELMV
                     TCDDPEEKKEYINIIQSNNELLLRLINDILDLSKIESGIIERRKENFNLAKVCNELFVT
                     IQAKMTNPNVELRLDGPNSECWVLLDRNRLKQVWMNFLTNAVKCTKSGYIKMGYGIERE
                     GLRIYVEDTGIGIPDDLHDKVFTRFEKLNEFSQGTGLGLTISRAIVEAAGGEVGFTSKL
                     GVGSTFWAWLPLRP"
     CDS             6545..6931
                     /locus_tag="ctg189_5"
                     /transl_table=11
                     /translation="MRFTRLILALTSFLILITQTDLFASTVLHKTSMVDLSNNAIICMH
                     QDPDGYLWIGTYDGLNLYNGKDTYVYRFELNNKNSLCSNIIHKISDAEPGFLWISTSLG
                     INKFSLKERKVTESFPGYMECDLLA"
ORIGIN
        1 ttccgataca aaaatataac atatttcttc attaaaacaa tttttctaat cgatatatct
       61 tatgaggtta tagataatac tttatcaatc aataaaaagg gcctatcctc acggagaagc
      121 ccttcaatat cttgggtttt ggattaccgc atcagtaacg attataacac tcccgcccgc
      181 acccggctca aagattcggg tgtcatcaat aaatacgagg cgatatgatt gacagaagca
      241 cgtttcgctg cttccggata ttcctgttga aaacgcagat aacgttttct tgccgactca
      301 aaacgccaag agtccgcttt ctcttgcgaa agcatcaaag cccactccag tattttctgg
      361 tacaatctat taatagctaa agatgttttc aataaatcgc taaattccga aaaaaagatc
      421 cggtgataaa ccgtcggttc caatgcctcg attaataaac gcgtcggttc tctttggaat
      481 gtgctggcta tacaaataac gatatttccc tcacaggaga aatgctcggt aatatcacgt
      541 ttattcttat agtaaaactg gcgtaataat cccgattcca cataatataa atatttagat
      601 atttgtccgt tatccaacaa taattctcct ttcgcagcct tacttgattc tatgatcgaa
      661 gccagagaat ccaaatccgc atcgggtaat ggataaaaac gtttggatat catttcggct
      721 atttcttgtg gtgtcttcat actattcatc attttctgat ctatatcatt ttttatctcc
      781 ttacaaaaat agatatttgt tgcatataac aaaacaaaca catattaaat agtcaaggtt
      841 agtctaggca aaaaattatt actatcgttt aaaagctaat cggattagat tgttttccct
      901 gattagctgt tgcataatcg caaggcttga tcgagagatc aggccttgtt attttctggt
      961 ttgtagcgag agaatatcgc tttcgcatta tctaaacgtt cttttgataa gggttccacc
     1021 ccctttaacg gataatcgag gcctaattgc tcatatttat aggttcccat cgtatgataa
     1081 ggcaataatt ccactttgcg aaccacttta tatgaactga cataccgagc taacgcctcc
     1141 agccactcgt cattatccgt atatccgggg acaataacat ggcgaatcca agtatctatg
     1201 ccacgtctct ccaattcatc caagaacaga agcgtattat cctgcttgac acccgccagt
     1261 aacggatgta aatccgggtt taaggttttg atgtccaata acactaagtc cgcataatcc
     1321 aagacttccc aagcttttga ggtacatacg aaaccagatg tgtccaaagc tgtgtgaaga
     1381 ccttgctctt ggcacaaacg gaaaaactct ttcagaaact caggttgcaa taaaggttcc
     1441 ccaccggtaa cagtcacccc cccacgggcg ataaaactct tataccgtaa gacttccgtc
     1501 aatagctcgc cgggagtcat ctgatatttc accttccctt tcgggttcca agtatccggg
     1561 ttatggcaat acaggcaacg caacgggcag ccctgcataa aaacgacaaa gcgaattccg
     1621 ggaccatcta cggtcccgaa actctctaat gaatgtatct taccttttat cacttacagt
     1681 gaatggttaa tggttcttga aataacatcc aactgttgtt cacgagtcag tttcacgaag
     1741 tttaccgcat atccggatac acggatcgta agttgcggat atttctcggg atgctccata
     1801 gcgtcgatca acaaatcctt atcaaatacg ttaacattca gatgctgacc tccatccgga
     1861 gtaaaatagc catccaataa gctaaccaga ttattgatct gcacttcacg ctcctttccc
     1921 aacgtagccg gagaaacagc gaaagtataa gagatgccgt catgcgcatg ctggaatgga
     1981 agcttcgcta ccgaagctag cgcagcgata gcacctttcg tatcacgacc gttcataggg
     2041 ttcgcacccg gagcgaaagg agttccaccg atacggccat ccggtgtcgt accagtcttc
     2101 ttaccatata ccacattaga ggtgatcgta aggatggact gcgtaggttt cgcatggcga
     2161 taggtctcgt gctgacgcaa atattccatg aacttctcgg tgatctctac agcgatacta
     2221 tccgtacgat catcgttatt accgaaagga acatattcgc cctcacgttc gaaatcaacc
     2281 gccaagccac gctcgtcacg gatcacacgt actttcgtat cacggatagc agccaacgaa
     2341 tcggcaacga tagaaagacc ggcgatacct gtcgcacgga tacgctctac atccccatcg
     2401 tgaagcgaca tctcgaatgc ctcatacgcg tatttatcat gcatataatg gattatcttc
     2461 aaggcgttca catacacttt cgccaaccag cgcatcattt gctcatactt agccatcacc
     2521 tcatcgtatt ccagatactc gccgcgaacc ggctcgaaca tcggggctac ctgtacgccg
     2581 ctgcgctcat cccgtcctcc gttgatcgca tataataaac atttcgccaa attcgcacgg
     2641 gctccgaaga actgcatctg cttaccgatc ttcataggag aaacgcaaca agcgatacca
     2701 taatcatctc cataatccgg acgcatcaaa tcgtcgttct catattggat agccgacgta
     2761 tcgatagaga ccttggcaca gaaacgtttc cagttctcgg gagcgttctt aaaccacaga
     2821 accgtcaaat tgggttctgg tgccggacct agattatata atgtatggag ataacgatag
     2881 gaagttcttg ttaccaaaga acggccatcc actccttggc cgcccaagga ctctgttacc
     2941 caaaccggat caccggagaa caaatcattg tattccggcg tacgtaagaa acgaacgata
     3001 cgcaacttga tgatcagctg gtctaccaat tcttgcgcct cctcttcggt caacttaccc
     3061 tcacggatgt ctttctcgat ataaatatcc aaaaacgtag aagtacgtcc gatagacatc
     3121 gccgcgccat cttggtcttt taccgccgcc agataagcta aatacacaaa ctgaaccgcc
     3181 tcacgagcgt tttgtgccgg acgagtcaca tcaaagccat agctagcgca cattttctcg
     3241 aacgctttca aagccttgat ctgttcggaa atctcctcac ggctctggat gatctcttcc
     3301 gtgatctctt ggatatccaa acgattcaag aaacgctttt tctcctcgat caagattgcc
     3361 gtaccataca gagcgacacg acgatagtca ccaataatac gtcctctacc atacgcgtcg
     3421 ggcagaccgg tgataatagc cgaacgacgt gcggcaatca tctcttccgt atatgcggag
     3481 aaaacgccct cgttatgcgt cttacggtac ttcgtgaaga tctctttcgt catcgggtcc
     3541 aaatgataac cataagcttc caagctattc tcgaccatac ggataccgcc tttcgggaag
     3601 ataccacgtt tcagcggagc atcggtttgt aacccaacga tcacctcatt ctccttatca
     3661 atatagcccg ggccatatac atcgatccct tgaggtaatt ttgtctcggc gtcataaact
     3721 cccttttccc tctcgacctt aaacatctcg gtcagcttgt tccagacctt acgggtcttc
     3781 tcggaagaag cgactaaaaa ggaatcatcc ccttcataag gcgtatagtt actctggata
     3841 aaatctctta cattgatctc tcttttccag gtctcaccct taaagtgatc ccagcactca
     3901 ttctcaaatt tcataaacaa tcttatctaa taccacattt ttaccgccgc aaaggtagga
     3961 gaattatcgt catatttctg tcacatatgc tacaccagtt ctaaaatccc tataaattgc
     4021 gataaaaaag cttctcatcc ttcactattt taaaaggaca ttaccaataa atggggagtc
     4081 agacctaagg gcgaagaggc aaccatgccc aaaaagtaga tccgacacca agttttgaag
     4141 tgaagcccac ttctccccct gccgcttcta cgatagctct ggagatcgtc aagccaagtc
     4201 cggtaccctg cgagaactca ttcaatttct caaaacgggt aaatactttg tcatgcaaat
     4261 catccggtat accgattccc gtatcctcaa catagatacg aagaccttcc ctttcaatgc
     4321 catatcccat tttaatatat ccggatttgg tgcattttac cgcattagtg aggaaattca
     4381 tccatacttg cttaagccgg tttctatcca gaagtaccca gcactccgag ttgggaccat
     4441 ctaaacgtaa ctcgacattc ggatttgtca tcttcgcctg aatcgtaaca aataactcat
     4501 tacaaacctt ggccagattg aaattctcct tcctacgttc aattatgcct gactctatct
     4561 tggaaagatc aaggatatcg ttgatcaatc gcaataacaa ctcattattg gattggatga
     4621 tatttatata ttcttttttt tcttccggat catcacaagt taccattaac tctgaaaaac
     4681 caacaatcgc gtttaacggg gtacgtatct cgtgactcat attagcgaga aacgcagact
     4741 tgagacgatc tgaaagttcc gctttctcct ttaattcctt caattgctga atcatcttct
     4801 cccacttcgt attattgatc gtaataccgg tatagcgagt cacatggcct tctttatctc
     4861 tttcaacctg gattccagta accaataagg tctgccactc gtcatcccat ttggttttgg
     4921 aacgatattt aacgttgaaa tctttatcta tgccccggaa catatgctcc atatatttcc
     4981 gcacaaaaac cacatcatcc ggatgtgtag cgttcaaata atcctcggga gtgattgtct
     5041 tttccgaata aaaatcattg atgggatcat taaaagcact gaatttcagg ctttttacat
     5101 caaaatccca atacgacata ccaaccgtct tgaaagaaag ctctatcttg taatttcttt
     5161 ctttaagttc ccgattcaat tgctggatct taggaatgtt ttgcctgaat cccgtatagc
     5221 gtattatatc tccattttca tcttgatcga aagggacacc cataaaatca caatactgcc
     5281 aagtctcatc atacttggtt tgtatgcgac aggtaaaatt gatggtgaca ttctttccag
     5341 ctatcaggga ttgcattgcg tcgtaaaaaa cagaccggtc ctcggggtat ataacatcca
     5401 tatactccga aacggtcaat aaccgatctg aaacgtaatc gttgatcgga tcattataag
     5461 attcgaattt ctgggaattt acatcaaaat cccaatgaac aatattactg actttcatgg
     5521 ccagatcacg ttttgcgatc agatcctgcg ctttcttggc catttgcaac ctttcggtta
     5581 tatccatttg tgtcccgatg atctgcaatt ttccacgatg ttctgtcgat aggcgcatcc
     5641 ggctctcata ataacgatac atctcttctt gctcgttata gaaacgcaaa gtcatttgtc
     5701 cttgttctat ctcatttcct atcaattgag agaagagccg gattaacgga gctcgatctt
     5761 ggggatgcaa aatatccagt accgcctcca ttggcatatt ttttttggct acggttagtc
     5821 catataatgg attaaaaacc tttttattca catcatatac ccaagaagcc atattagccg
     5881 ctttcatggc catctccaga tttttcttgc tttcttccgt acggaattcc acatctttca
     5941 cggaagtcat atcatttgcg agtaatatat gtccgataag atgttttttt ctactccata
     6001 tcgggataac tttaacccca tagatcatcg tgtctttatt atgggaagaa taatatgcat
     6061 ccttattaac ccgatcaaaa tcatactcaa actccaagga aatatcttca cccgattgta
     6121 tccgagctaa aagttctgaa tcaacaaatg gacttttgta aagattgact atattcacca
     6181 ccgatgtccg gtcgtccaca ccatacatcc tcaacgcatg gtcgtttata ctacgcagca
     6241 caccatgtgc gtcataaatt tcaataccca ccggcagaca agcatatata ctctccgtaa
     6301 ataaacgctg ttcttctatt aatttagaaa tttctttctc tattatcaca tcgtcatttt
     6361 cttgagaatt ctcttcacat tgttccatct gtcacttact ttatttcgta ttatcatacg
     6421 tcagacattc taatttatac gaaagccctg ctttttgcaa aagtaaacaa aaaagtgaat
     6481 tcttttctgt tcgttcataa agatattatc tttgcgctga aaacaaacac taaatcttaa
     6541 atacatgaga ttcacaaggc ttattctagc gttaaccagt ttccttatat tgatcacgca
     6601 aacagaccta tttgcctcaa ccgtattgca taaaacttct atggttgatc tctcaaataa
     6661 cgctattatt tgcatgcatc aagatccgga tggctatttg tggatcggga cttacgacgg
     6721 acttaactta tacaacggaa aagatacgta tgtatatcgt ttcgaattaa ataacaaaaa
     6781 ctctctttgt agtaatatca ttcacaaaat atccgacgca gaacccggct ttttatggat
     6841 atcaacctct ttaggtatca ataaattctc actaaaagaa cggaaagtca ccgagtcttt
     6901 ccccggatac atggaatgcg atctattagc gtc
//
