LOCUS       c00320_gnlPro..         4830 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KGGOFMJL_320.
ACCESSION   c00320_gnlPro..
VERSION     c00320_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 11:27:56
            Original ID  :: gnl|Prokka|KGGOFMJL_320
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4830
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4830
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:2114]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..2114
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4830
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4830
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..1372
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg320_1"
                     /sec_met_domain="Gly_radical (E-value: 2e-47, bitscore:
                     149.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 2e-198, bitscore:
                     649.3, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="SFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLN
                     DGRTKVTKTSFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYEND
                     DLMREVRQSDDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKN
                     IPVLTSDTLKFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRI
                     NLAYGVAGLSIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVD
                     LVYFFSEELKKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHG
                     RDKNGAIASLSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGA
                     HHLNVNVLNRDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             1386..2114
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.5;
                     E-value: 1.4e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg320_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.3e-29, bitscore:
                     92.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(2107..2556)
                     /locus_tag="ctg320_3"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDKDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(2543..2710)
                     /locus_tag="ctg320_4"
                     /transl_table=11
                     /translation="MKEIVSRILDVIMYLIPFFGKRKRDRVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             complement(2770..3273)
                     /locus_tag="ctg320_5"
                     /transl_table=11
                     /translation="MAMTVSYSVVPRKNPAKKSEPAKYYAQAQASGELDFEELCEAITS
                     RSTCTETDVRAAISGILYEVKRALKAGRIARLGDLGSLQIGLNSEGAASVKEFSGSMIK
                     GAHLIFRPGKTLAELMKILSYQQVQTRAVAQAGAGDGGGEDPDKNPDSGGDGSGDEEAP
                     DPTV"
     CDS             3467..3697
                     /locus_tag="ctg320_6"
                     /transl_table=11
                     /translation="MNYKEDSFLIRTYTKAELAHLYNPHVCLKVALQILRRWIIYNLPL
                     LHELEQEGYRARNRLLSPKQVATIIHFLGEP"
     CDS             3784..4830
                     /locus_tag="ctg320_7"
                     /transl_table=11
                     /translation="MKHFTIFQGFYYAIAEMTEEEIVSTIGSFTYREKVEEIRRIFAEQ
                     GEKAANEKKKELPAIAFSASYRGRRTKVNLVKYLGHIVIDIDHLSKEELARILPIIKRC
                     DYTRIAFISPKGMGVKIIVRACHPDETLPETLQEIEDFHHAAYTRLVSFYTELCQIEID
                     TSGQDVARTCLFSYDPEIYFNPNADAFLVDQPQASYKVSNRKNLSGSKQQTPPDGPPTN
                     EDTALNAHSANASLVLTLTYYHNKSEKYIVGNRNNYLHHLSCTFNRYGIPQEEASAFIK
                     SQFTDFPADETASLINSAYAHTDEFNTCKLNGTQKRILRIEQYISEHYETRYNEVLHIM
                     EYRRRHPDT"
ORIGIN
        1 atcattcgcg caagagctga tcgaccagtt tgtcatcaaa ctgcgtatgg tccgccacct
       61 gcgtatgcaa tcatacaacg acatctttgc aggcgacccg acctgggtaa ccgaatctct
      121 gggcggacgt ctcaacgacg gacgtaccaa agtgacaaag acctccttcc gtttcctgca
      181 gacattgtac aacctcggcc cttcaccgga accgaacctg accgtactct ggagcccgga
      241 acttccggaa ggattcaaag aattctgtgc aaaggtttct atcgacactt cttctatcca
      301 gtacgaaaat gacgacctga tgcgcgaagt acgtcagtcc gacgactacg gaatcgcctg
      361 ctgcgtatca taccaggaaa taggtaagca gattcagttc ttcggtgccc gttgcaacct
      421 ggccaaagcc ctgttgcttg ccatcaacgg cggacgttgc gagaataccg gtacagtcat
      481 ggtgaagaac atccccgtac tgaccagtga cacgctgaag ttcgaagaag taatggacaa
      541 ctacaaaaaa gtgctgaccg aaatcgcccg cgtatataac gaagcgatga acatcatcca
      601 ctatatgcac gacaagtatt actacgagaa ggcccaaatg gctcttgtag atacgaaccc
      661 gcgcatcaac ctagcttacg gtgtagccgg actttccatc gccctcgatt cactgtctgc
      721 catcaaatat gcgaaagtaa ctgcccgccg caatgatatc ggtctgacag aaggcttcga
      781 catcgaagga gaattccctt gcttcggtaa cgacaacgac aaggtagacc accttggcgt
      841 agacctggta tacttcttca gcgaagaatt gaagaaattg cctgtttaca agaatgcccg
      901 tcctaccctc tccttgctga ctatcacttc caatgtgatg tacggcaaaa agaccggtgc
      961 tactcccgac ggacgtgcca aaggtgttgc cttcgctccg ggtgccaacc cgatgcacgg
     1021 acgcgacaag aatggtgcga tcgcttctct gagttctgta gcgaaacttc gttaccgtga
     1081 ctcacaggac ggtatcagca acaccttctc tatcgttccg aaatcactgg gtgctacgga
     1141 cgaagaccgt atcgagaacc tcgtaacgat gatggacggt tacttcacca aaggtgccca
     1201 ccacctgaac gtaaacgtac tgaaccgtga tatgctgtac gatgccatgg agcatccgga
     1261 aaactatccg cagctgacta tccgcgtttc cggctatgcc gtaaacttcg tgaagctgag
     1321 ccgcgaacac caactggaag ttatcagccg tagcttccac gaacgtatgt aatctattac
     1381 tctatatgat gataaacgta cattcatacg aaagtatggg aacattcgac gggccgggct
     1441 tacggctcgt cgtttttctt caaggatgca acttccgttg tctctattgc gccaatccgg
     1501 atacgatagc aggaaaggga ggcacgccta ccccaccgga agaaatcgtc cgcatggcaa
     1561 tgagccagcg ccccttcttc gggaaacgag gaggaatcac tttctccgga ggcgaaccga
     1621 cgtttcaggc gaaagcactc gtcccgctgg tccgcgaact gaaagaaaaa ggcatccatg
     1681 tctgtctgga cagcaacgga ggactttgga acgaagacgt agaagaatta ttcagactga
     1741 ccgatcttgt gttattggat ataaaggaat tcaaccctaa ccgccatcaa acgctcaccg
     1801 gaagaagcaa cgagcaaacc atccgcacgg ccgcctggct ggaagaacag gagaaacctt
     1861 tctggctacg ctacgtgcta gtgcccggat acagcgattt tgaagaagac atacgcgcac
     1921 tgggagaagc actcggaaaa tacaagatga tccaacgggt ggaaatactg ccttaccata
     1981 cactgggcgt acataagtac gaagcgatgg gacaggaata caagatgaaa ggggtaaaag
     2041 aaaatacccc cgaacagctt gaaaaagctg cggaggtatt caaagagtat ttcactacgg
     2101 tggtagttaa ttaatggaga attctccaat gacctcaaag caagggcatt ctttaatccg
     2161 ttcccaagga tctacgatcc cattatagtt ggtgtcggga cttaaatccc gatgccctac
     2221 tactttagct tccggataac gctgcaacaa ttcgtgtacc aagcgatgta gcgcctcctt
     2281 ctgacggggt gtacgcgtat ccgcagcttt tccattctca tccagaccgc cctcataaca
     2341 tacacctaag ctgatcgcat tatagccacg tgcatgagct ccgaccgtat ctttatcccg
     2401 catcggctca atctcgcccg acttgcgtat ataataatga tacccccagc acgaaaagcc
     2461 acgataacgg tgagccgtat ccacatcctt tgccgtaaag tcgcaatcga cacgggtggc
     2521 ggaacaatgg atcacaatca gactaatctt tctcatgttc cttttccctt tccgtcgttt
     2581 tcactttgca cacctcctta caagtagtat gatagcgcac ttctctcacc accctgtccc
     2641 gctttctctt tccgaagaaa ggaatcagat acatgatcac atctaaaatc cgacttacta
     2701 tttctttcat cttatcaaaa tattaatgga agttattcta attcgttatt accttgaccc
     2761 gtctgccaat tataccgtag ggtccggagc ctcttcatca ccggagccat caccaccgga
     2821 atccggattc ttgtcagggt cttctccccc gccatcgcct gcacccgcct gcgccaccgc
     2881 acgagtctgc acttgctgat aactcagaat cttcatcaac tccgccagcg tcttaccggg
     2941 gcggaaaata agatgcgccc ccttaatcat tgaaccggaa aattccttga ccgatgccgc
     3001 accttcactg ttgagtccta tctgcaaact gcccaaatcg cccagtctcg caattcttcc
     3061 tgccttcaac gcacgcttca cttcgtaaag aatacccgaa atagccgcac gtacatccgt
     3121 ttccgtacaa gtagaacgac tggtgatcgc ttcgcacaac tcttcaaagt ccaactctcc
     3181 cgacgcctgc gcctgtgcat aatactttgc cggttcgctc ttttttgcag gattcttgcg
     3241 cggcaccact gaataagata ctgtcattgc cataataaaa taattttaaa gtttataata
     3301 cataggatgc atccccttct tgttaacagc acaaagataa ggatagccgg acaagccata
     3361 ttccccaacg aagtttcttg attcccaacg aataaaaacg aagtcaaacc tttgaataac
     3421 aaggaatttt atgtaacttt atagcataaa acaagaagaa aacatcatga attacaaaga
     3481 agattctttc cttatccgca cctataccaa agcggaactg gctcaccttt acaatcccca
     3541 tgtctgcctc aaagttgctt tacaaatact tcgtcgttgg attatttaca acctccctct
     3601 cctgcacgaa ctcgaacagg aaggctaccg cgcacgcaac cgtctccttt cgccgaaaca
     3661 agtggcaact atcatacact ttttgggcga accgtagtaa cttattgcca ttttatacaa
     3721 agttccttta cacatttccg caaagaagtt cctttgcatc gacaaacaat aatcaaaata
     3781 aaaatgaaac atttcaccat atttcaaggt ttctactacg caatagcaga gatgaccgaa
     3841 gaagaaatag tgtccactat cggcagtttt acctaccggg aaaaagtcga agaaatacgc
     3901 cgcatctttg cagagcaagg agaaaaggct gccaatgaga agaaaaaaga attgccggca
     3961 atcgccttct ccgccagcta ccgaggaagg cgtaccaaag tgaacctcgt caaatatctg
     4021 ggacacatcg tgatagacat cgaccacctc agcaaagaag aattggcacg catcctccct
     4081 atcatcaaga gatgcgatta cacccgcatc gcctttatca gccccaaagg gatgggcgtg
     4141 aaaatcatag tacgcgcatg ccatccggat gagacattgc ccgaaaccct tcaggaaata
     4201 gaagacttcc atcatgcagc ttacaccaga ctcgtttctt tttacaccga actctgtcag
     4261 atagaaatcg acacctccgg tcaggatgtg gcacgcactt gtctgttctc ttacgatccg
     4321 gagatttatt tcaatccgaa cgctgacgct tttcttgtag atcaacctca ggcatcctac
     4381 aaggtttcaa atcgtaaaaa tctctccggc agtaaacaac agactcctcc ggacggcccc
     4441 ccgacgaatg aagacacagc gctcaacgcc cattccgcca acgcttctct ggtgctgact
     4501 ttaacctact atcacaacaa gtccgaaaaa tacatagtgg gcaaccggaa caattatctg
     4561 caccatctct catgtacctt caatcgctac ggcattccgc aggaggaagc atccgccttt
     4621 atcaaaagcc agtttacaga ctttccggcg gacgagacag cctctttgat caatagcgcc
     4681 tacgctcata ccgacgaatt taatacctgt aagctaaatg gcacgcagaa acgaatatta
     4741 cggatagagc aatatatcag cgagcactac gaaacacgct acaacgaagt gctgcatatc
     4801 atggaatatc gcagaaggca tcccgacacc
//
