LOCUS       gnlProkkaALINBPNL_306   5337 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ALINBPNL_306.
ACCESSION   gnlProkkaALINBPNL_306
VERSION     gnlProkkaALINBPNL_306
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##antiSMASH-Data-START##
            Version      :: 7.1.0
            Run date     :: 2024-11-18 05:52:58
            Original ID  :: gnl|Prokka|ALINBPNL_306
            NOTE: This is a single region extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5337
            ##antiSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5337
                     /aStool="rule-based-clusters"
                     /category="other"
                     /contig_edge="True"
                     /core_location="[452:4780]"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
                     /tool="antismash"
     proto_core      453..4780
                     /aStool="rule-based-clusters"
                     /tool="antismash"
                     /cutoff="20000"
                     /detection_rule="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /neighbourhood="5000"
                     /product="phosphonate"
                     /protocluster_number="1"
     cand_cluster    1..5337
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(phosphonates and minimum(1,
                     [2-Hacid_dh_C, Aldedh, Aminotran_1_2, Aminotran_5, AurF,
                     CTP_transf_like, DUF4992, Fe-ADH, HMGL-like, Metallophos_2,
                     NTP_transf_3, NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C,
                     TPP_enzyme_N, phosphonates]) and not (phosphonatesHydrolase
                     or phosphonatesMeIsocitrateLyase))"
                     /kind="single"
                     /product="phosphonate"
                     /protoclusters="1"
                     /tool="antismash"
     region          1..5337
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="phosphonate"
                     /region_number="1"
                     /rules="(phosphonates and minimum(1, [2-Hacid_dh_C, Aldedh,
                     Aminotran_1_2, Aminotran_5, AurF, CTP_transf_like, DUF4992,
                     Fe-ADH, HMGL-like, Metallophos_2, NTP_transf_3,
                     NTP_transf_5, PALP, PEP_mutase, TPP_enzyme_C, TPP_enzyme_N,
                     phosphonates]) and not (phosphonatesHydrolase or
                     phosphonatesMeIsocitrateLyase))"
                     /tool="antismash"
     CDS             complement(3..110)
                     /locus_tag="ctg306_1"
                     /transl_table=11
                     /translation="MENLKQKTVSGIMWSAIERFSLQGVQFIIQIILARL"
     CDS             complement(313..456)
                     /locus_tag="ctg306_2"
                     /transl_table=11
                     /translation="MIAEILLKIRHFYFSLRGIHFHPESVISGVPIRSFRLVGKYGNIN
                     LR"
     CDS             complement(453..1574)
                     /NRPS_PKS="Domain: Aminotran_5 (30-366). E-value: 2.9e-34.
                     Score: 110.4. Matches aSDomain:
                     nrpspksdomains_ctg306_3_Aminotran_5.1"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: Aminotran_5"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1139:aminotransferase class V (Score: 76.3; E-value:
                     3.6e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg306_3"
                     /sec_met_domain="Aminotran_5 (E-value: 9.5e-32, bitscore:
                     99.3, seeds: 34, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKIERKILLNPGPATTTDSVKMAQVVPDICPREKEFAGMMKQLRD
                     DLVRVAHGNLEKHTAVLFCGSGTINIDICLNSLLPEDKRVLIVNNGAYSTRAVEVCQYY
                     GLPHINLEFSVYERPDLSVVENVLQENPDIAMVYTTHHETGTGILNPIREMGAIAHKYQ
                     AKFVVDTTSSLGMIPFDIEKDNVDFCMASAQKGLQAMTGLSFIIGNEEQIKFSKKYPKR
                     SYYCNLYLQYENFERTGEMHFTPPVQTIYATRQALNEYFEVGEEAKFARHKRVFEAIHS
                     GINEIGLKSVIKREWQSGLVVSVQYPEDPNWDFEKIHDYCYERGFTIYPGKISTEDTFR
                     ICALGEIEVDDIENFFKVLKAALNHYNVTLPMS"
     aSDomain        complement(477..1484)
                     /aSDomain="Aminotran_5"
                     /aSTool="nrps_pks_domains"
                     /database="nrpspksdomains.hmm"
                     /detection="hmmscan"
                     /domain_id="nrpspksdomains_ctg306_3_Aminotran_5.1"
                     /evalue="2.90E-34"
                     /label="ctg306_3_Aminotran_5.1"
                     /locus_tag="ctg306_3"
                     /protein_end="366"
                     /protein_start="30"
                     /score="110.4"
                     /tool="antismash"
                     /translation="PREKEFAGMMKQLRDDLVRVAHGNLEKHTAVLFCGSGTINIDICL
                     NSLLPEDKRVLIVNNGAYSTRAVEVCQYYGLPHINLEFSVYERPDLSVVENVLQENPDI
                     AMVYTTHHETGTGILNPIREMGAIAHKYQAKFVVDTTSSLGMIPFDIEKDNVDFCMASA
                     QKGLQAMTGLSFIIGNEEQIKFSKKYPKRSYYCNLYLQYENFERTGEMHFTPPVQTIYA
                     TRQALNEYFEVGEEAKFARHKRVFEAIHSGINEIGLKSVIKREWQSGLVVSVQYPEDPN
                     WDFEKIHDYCYERGFTIYPGKISTEDTFRICALGEIEVDDIENFFKVLKAALNHY"
     CDS             complement(1584..2327)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1055:pyruvate oxidase/decarboxylase (Score: 60;
                     E-value: 3e-18)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg306_4"
                     /sec_met_domain="TPP_enzyme_C (E-value: 2.1e-21, bitscore:
                     65.4, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MRIPYIILDSNENDAFIQLHDIIQSAKTKNIPHAIIIRKDVFGKY
                     KLQKEFGCNYPLSREDALQIVVDYLPENSVVVSTTGKLSRELFEYREMKEQGHEHDFLT
                     VGSMGHSSSISLGIAIAKQDRPVYCLDGDGAFIMHLGAISNIGDLSPKNYYHILFNNGA
                     HESVGGQPTLGFSLDIPAIVRGSGYKHTYTVCTKVEIEEAMKQLPKLCGPVLLEIKVKI
                     DSRENLGRPTTTPIENKEHFMDFLK"
     CDS             complement(2348..2713)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: TPP_enzyme_N"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg306_5"
                     /sec_met_domain="TPP_enzyme_N (E-value: 1.7e-19, bitscore:
                     59.1, seeds: 75, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLSVEKVYETFLSHDVDFFTGVPDSLLKNICAYITDHTSREKHII
                     AANEGAAVGIASGYYMASGKVPVVYMQNSGLGNTVNPLLSLADEQVYSFPMLLMIGWRG
                     EPGTKDEPQXXXXNRVR"
     CDS             complement(2730..4040)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: phosphonates"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: PEP_mutase"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: CTP_transf_like"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1231:carboxyvinyl-carboxyphosphonate (Score: 198.6;
                     E-value: 2e-60)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg306_6"
                     /sec_met_domain="CTP_transf_like (E-value: 1.2e-13,
                     bitscore: 40.6, seeds: 65, tool: rule-based-clusters)"
                     /sec_met_domain="phosphonates (E-value: 1.3e-118, bitscore:
                     384.4, seeds: 8, tool: rule-based-clusters)"
                     /sec_met_domain="PEP_mutase (E-value: 1.4e-60, bitscore:
                     193.8, seeds: 69, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKTVYIGMTADIMHPGLIRIINEATKYGDVIIGLLTDKAIAEHK
                     RLPYLTYEQRKEVVQNIKGVCKVVPQEEWSYVENLKRIKPDYIIHGDDWKTGPLREERV
                     RVFEVMNEQGGKVIEIPYTLGINSSSLDKDIKAIGTTPDVRLKSLRRLINAKPVVRILE
                     AHDGLCGLIIENQEILKGDKREVFDGMWSSSLTDSTSKGKPDIEAVDLTTRLQDLNNIL
                     ECTTKPIIFDGDTGGKIEHFVFTVRTLERHGIXXXXAIIIEDKVGLKKNSLFGTDVIQT
                     QDTIEGFCNKIKAGKASQITDDFMIIARIESLIAGKPVSDALERAFAYVQAGADGIMIH
                     SKNKSGEDIKEFCLAFRKQYAHVPIVVVPTTYDHIYESELCDWGVNIIIYANHMLRAAY
                     PAMMNVAKIILENERALEVRELCMPIKEILELIPGTK"
     CDS             complement(4061..4780)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     phosphonate: NTP_transf_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1064:glucose-1-phosphate
                     adenylyl/thymidylyltransferase (Score: 54.5; E-value:
                     1.6e-16)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg306_7"
                     /sec_met_domain="NTP_transf_3 (E-value: 1e-16, bitscore:
                     50.8, seeds: 297, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MIRTAMIMAAGMGTRFGQYTELIPKGFVKVGGIPMIVRSIDTLLS
                     CDIERIVIGTGYKQEVYEELKTDYPMLETCFSPRYAETNSMYTLYNTREILGNDDFLLL
                     ESDLIFEKQAIMSLLECPAADAMLITPVTKFQDQYYVEHDDNFRLSSCSVNKNKLNAKG
                     ELVGIHKLSGSFYKIMCADYASIVDEQPNLGYEYELLRISCSQSPVYVHKVEGLKWYEI
                     DDISDLEYAEKYIVPYC"
     CDS             complement(5085..5336)
                     /locus_tag="ctg306_8"
                     /transl_table=11
                     /translation="MVIADSVVHKLQEYIIGYRTSKVKEDCAYLERLFKERQQEYYAAQ
                     RKYADYVDAHDNVVLQSVRAEQERLQNDXXXXXGLSDI"
ORIGIN
        1 aacaaacgag ctaatattat ttgaattata aactgtactc cttgtaaaga gaaacgttct
       61 atagcgctcc acattattcc tgatacagtt ttctgcttta gattttccat tacttaaata
      121 cagtataaaa acaactacat ttaccgatat caattatatt tttccaatat tctcactaat
      181 tggttattat cttcttcgcc ccttaccgca attctaatat ggtctctccc actaaaagca
      241 aattacccta cacaaacatt atctcctata actaccgatg cctttttata agaataaaga
      301 actcctaatg catcacctca aattaatatt gccatatttg cctactaagc gaaagctacg
      361 aataggcact ccagaaataa cactttccgg atggaagtgt ataccacgaa gggaaaaata
      421 aaaatgacga atcttcaata atatttctgc aatcatgaca tcggtaatgt gacgttataa
      481 tgatttaatg cagcttttag tactttaaag aagttctcaa tatcatctac ttctatctct
      541 cctaaagcac agattctaaa agtatcctca gttgatatct tacctggata aatcgtaaat
      601 ccacgctcat aacaataatc atgtattttc tcaaaatccc agttgggatc ttctgggtat
      661 tgaactgaga caaccagtcc tgactgccat tcacgcttta ttacactttt aagaccaatt
      721 tcatttatac ctgaatgaat agcctcaaaa actcttttgt gacgggcaaa cttagcttct
      781 tcacccactt caaaatattc atttaatgcc tgacgagtgg cgtaaatagt ttgtaccggt
      841 ggagtaaaat gcatttcacc tgttctctca aaattctcat actgcaaata aagattacaa
      901 tagtaagaac gcttgggata tttttttgaa aacttaatct gttcctcatt gcctataata
      961 aaagaaagac ctgtcatcgc ttgaagtcct ttttgagcag atgccataca aaaatccaca
     1021 ttatctttct ctatatcaaa aggaatcatg cccaaagaag aagtagtatc gacgacaaat
     1081 ttagcctgat atttatgagc tatagcgccc atctcgcgaa tagggttcag aattcctgta
     1141 cccgtctcgt gatgagtagt ataaaccata gctatatcag gattttcttg caacacattt
     1201 tccaccaccg ataaatcagg acgttcgtaa acggaaaatt ccaaattaat atgaggcaaa
     1261 ccataatatt gacacacttc tacagcacgg gtactatatg ccccattatt cactataagc
     1321 acccttttgt cttcaggaag aagagaattt agacatatat caatattgat ggttcctgag
     1381 ccacaaaata atacagcggt atgtttttcc aaattcccat gagcaactct gaccaaatcg
     1441 tcccgaagtt gtttcatcat acctgcgaat tctttctcgc gaggacaaat atccggaaca
     1501 acttgtgcca tttttacact gtcagtagta gtggcaggcc caggatttaa taatattttt
     1561 ctttctattt tcatcgaata tatttatttt aaaaaatcca taaaatgctc cttattctct
     1621 ataggagtag tcgtgggacg tcccaaattc tcccgagaat ctattttaac ctttatttcc
     1681 aacaaaacag gaccacataa ctttggaagc tgcttcattg cttcttcaat ttcaactttt
     1741 gtacaaacag tataagtatg cttatatccg ctcccccgga caatagccgg aatatctaaa
     1801 ctaaatccta aagtaggctg tcctcctaca gactcgtgag caccattgtt aaacaaaata
     1861 tgataatagt ttttaggaga taaatcgcca atgttactaa ttgcccctaa atgcataata
     1921 aatgctccat caccatccaa gcaataaaca ggtctatctt gctttgctat agcaataccc
     1981 aaagagattg atgaactatg tcccatagag cctaccgtga gaaaatcatg ttcatgacct
     2041 tgctctttca tttctcgata ttcaaataac tcacgagaaa gcttgccagt agtagaaacg
     2101 accactgaat tttcaggcag ataatccaca actatttgca aagcatcctc acgagataaa
     2161 ggataattac atccgaactc tttttgaagt ttatatttgc caaaaacatc tttccgaata
     2221 attattgcat gaggaatatt cttagttttt gctgattgta tgatatcatg gagctgaata
     2281 aatgcatcat tctcatttga atcaagaatt atataaggaa tccgcattgc tttcaacaaa
     2341 tctaaagtta cctcaccctg ttttnnnnnn nnnnctgagg ttcatctttt gttcccggtt
     2401 caccacgcca gccaatcatc aaaagcattg gaaaactgta tacttgttca tctgcaagag
     2461 acagcagagg attaacagta ttacccaagc cggaattttg catataaacc acaggaacct
     2521 tacctgatgc catataataa cctgaagcaa ttcctacagc cgcaccttca ttagcagcta
     2581 tgatatgttt ctcacgtgaa gtatggtctg tgatataggc acaaatattt tttagtaatg
     2641 agtctggaac tcctgtaaaa aaatcaacgt catgactcag aaacgtttca taaacttttt
     2701 ctacgcttaa cataatgata ttcctctttt tatttagtac ctggaatcag ttccaatatt
     2761 tcttttatgg gcatacacag ttcgcgcacc tctaaagcac gttcattctc taatattatc
     2821 tttgcgacat tcatcatagc aggataagcg gcacgaagca tatgattagc ataaataatt
     2881 atattcacac cccaatcaca caattcagac tcataaatat gatcatatgt agtaggcaca
     2941 accacgatag gtacatgagc atactgtttt cgaaacgcca aacaaaattc ttttatatct
     3001 tcacccgact tatttttgct atgtatcata ataccgtccg ctcccgcttg gacatatgca
     3061 aaagctctct ctaaagcatc acttaccggc tttcccgcta taaggctctc aatacgagcg
     3121 ataatcataa aatcatcagt aatctgagat gcctttcctg ctttgatctt attacagaaa
     3181 ccttcgattg tatcttgagt ttgaataaca tctgtaccaa acaatgaatt cttttttaat
     3241 ccaactttat cttcaattat tatagctnnn nnnnnnnaaa ttccatgacg ttctaaagtg
     3301 cgtactgtaa atacgaaatg ttcaatttta ccacctgtat ctccatcaaa aataataggt
     3361 ttagtagtac actccagaat attattcaaa tcttgcaatc gggtagttaa atcaaccgcc
     3421 tcaatatcag gtttaccttt actggttgaa tccgtaaggc tactagacca cattccgtca
     3481 aatacttccc tcttatcccc ttttaatatt tcctgatttt caattatcaa gccacatagt
     3541 ccatcatgag cttccaaaat acgaactacg ggtttggcat ttatcaaacg acgaagtgat
     3601 tttaaacgta catcaggggt tgtacctata gccttaatat ctttatccaa agaagatgaa
     3661 ttaattccca aagtatatgg aatttcgata acctttcctc cctgttcatt catcacttca
     3721 aatacgcgca cacgttcttc acgtaaaggc ccggttttcc agtcgtcacc atgaattata
     3781 tagtcaggtt taatgcgttt gagattctca acatagctcc attcttcttg aggaacaact
     3841 ttacatacgc ctttaatatt ctgaactacc tcttttcgtt gttcataggt cagataagga
     3901 aggcgtttat gctccgcaat agctttatct gtaagaagac caattataac atcaccgtat
     3961 ttagtagcct cattaataat gcggataagt cctggatgca taatatccgc agtcatccca
     4021 atataaactg tcttactcat aatttgatat attattttaa ttaacaatac ggaacaatat
     4081 atttctctgc atattccaaa tctgatatgt catcgatttc ataccatttc agcccttcta
     4141 ctttatgcac ataaacagga gattgagaac aagatattcg tagtaattca tactcatatc
     4201 ccaaattggg ttgttcatca acaatactcg cataatcagc acacattatt ttgtaaaagg
     4261 aaccggatag tttatggatg ccgaccaatt cgcctttagc gtttagttta ttcttattga
     4321 cagaacatga agaaaggcga aaattatcat catgttccac ataatactga tcttgaaact
     4381 ttgtcactgg agttattagc attgcatctg ccgcaggaca ctccaataag ctcattattg
     4441 cttgtttctc aaaaatcaaa tctgactcta acaataaaaa atcatcattg cccagaatct
     4501 cccgcgtatt atataaagta tacatactat ttgtttcagc ataacgagga ctaaaacagg
     4561 tttcaagcat aggatagtct gtcttcaatt cttcataaac ttcttgttta tatcctgtac
     4621 cgattacaat tcgctcaatg tcacatgata acaaagtatc aatagaacga actatcattg
     4681 gaatacctcc cactttgacg aaccctttag gtatcaattc tgtatattgg ccaaaacggg
     4741 ttcccatgcc cgcagccatt atcatagctg tacgtatcat tcgtttaata atatagtaag
     4801 tgaatttaat tgatgtaaga atgttgtaaa cgacgtttta gcatcattta tcctgttcta
     4861 tctccttatt tttcagttct tttctcaagc tagccagcag acttttgcca aaaaacaccc
     4921 acacgagagt accagatacg gacaggaaca caaataataa aatataaatt ttgagtccca
     4981 tacctgaagg tttcaaaggt acaacggcag gctcaactac tgcaaagact ggcttttcct
     5041 cctgtacttt ggctctggat acctgcaact gattagcaac ctgactatat atctgataag
     5101 ccnnnnnnnn nnnnnntgtc gttctgtaaa cgctcctgct cagcacgtac actttgaaga
     5161 accacattat catgggcgtc tacataatcc gcatattttc tttgagcagc ataatattcc
     5221 tgctgacgtt ctttaaacaa ccgttccaaa tacgcacagt cttcttttac ttttgaagtt
     5281 cgatacccaa taatatattc ctgtaattta tgaacgacag aatctgctat aaccgca
//
