LOCUS       c00199_gnlPro..         2515 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ANFHKOOO_199.
ACCESSION   c00199_gnlPro..
VERSION     c00199_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 17:21:21
            Original ID  :: gnl|Prokka|ANFHKOOO_199
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2515
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2515
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1199:2514]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1200..2514
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2515
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2515
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..1099
                     /locus_tag="ctg199_1"
                     /transl_table=11
                     /translation="IEVNRGLETLIRESVLDVFWMHHRWKSVDRFAPQDKKTAALLENM
                     ELKPYRILVAVPGALDEALATVPLIRALKTVRCDMQVNVICPSAQMGIWKTVPEVTHVL
                     PHDSLKQLREALVADEFYNDGPLDMGVMLDGDMETLKALEPYGPMMFSGLDTHPGVRKY
                     KFRVKAPVLRAAPPAHRVQLYLQLGGLHGLDVGKPSLFPVKKAAPAEGAPILIAPFSRL
                     GSASEWSREQWTELVSLLPGRTVLVALEEDRERAAALAEHLNVELAVGAPEALFSVMDG
                     ASVAVAVDGDFPSLCSFRGLPVVTLFSTRLPDVYRPMGTFNRSLYSHQCCSPCFRKDCD
                     RDVPCNRHIAVREVLDALREISGKE"
     CDS             complement(1200..1979)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg199_2"
                     /sec_met_domain="Radical_SAM (E-value: 2.7e-31, bitscore:
                     96.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSAGDVLREIARYRDFLQAAGGGVTLSGGDPLFQPDFTGA
                     VLKGCRELGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPERYRSLTGGELRPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEGLSRHAALLGNVERVDVLPFHQMGRFKW
                     DELKLDYTLKGTPEASAKLAEQVRGIFRREGFPDCVH"
     CDS             complement(1996..2514)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg199_3"
                     /sec_met_domain="Gly_radical (E-value: 6.7e-48, bitscore:
                     149.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 1.8e-09, bitscore:
                     24.1, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="SNFMEKLRKLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAG
                     EPFAPGANPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKL
                     VSLLDAYFAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQE
                     VINRTFHTR"
ORIGIN
        1 tattgaagta aacagggggc tggaaacatt gatccgggaa tccgtgctgg atgtgttctg
       61 gatgcaccac cgctggaaat ccgttgaccg ttttgctccg caggataaaa aaacggctgc
      121 gcttctggaa aatatggagc tgaagccgta ccgcattctt gtagccgttc ccggggcgtt
      181 ggatgaagcc ctggcgaccg ttcccctgat tcgtgccctg aaaaccgtcc ggtgcgacat
      241 gcaggtgaac gttatctgcc cgtctgccca gatgggaata tggaagacgg tgccggaggt
      301 tacccatgtg ctgccgcacg attccctgaa gcagcttcgg gaagctttgg tggcagatga
      361 attttacaat gacggcccgc tggatatggg cgtcatgctg gatggagata tggaaaccct
      421 gaaagcgctg gaaccgtatg gccccatgat gttctccggg ctggacacgc atcccggcgt
      481 ccggaaatac aaattccggg taaaggcccc cgtcctgcgc gccgcccccc cggcgcaccg
      541 ggtgcagctt tatctgcaat tgggggggct gcatgggctg gacgtcggta aaccgtcttt
      601 gttccctgtg aaaaaagcgg ccccggcgga aggcgccccc attttaattg ctccgttttc
      661 ccgtctggga agcgccagtg aatggagccg ggagcagtgg acggaactgg tttccctttt
      721 gccgggacgg acggttctgg tggctctgga agaagaccgg gagcgcgccg ccgcgctggc
      781 ggaacatttg aatgtggaac tggctgtggg tgcgccggag gctctgtttt ccgtcatgga
      841 cggcgcttcg gtggcggtag ccgtggatgg cgatttcccg tccctctgtt ccttccgcgg
      901 gcttccggtg gtgactctgt tttccacccg gcttccggat gtgtaccgcc ctatgggtac
      961 attcaaccgt tccctttaca gccaccagtg ctgttccccc tgcttccgga aggattgtga
     1021 ccgggatgtc ccctgcaacc gccacattgc cgtgcgggaa gtgctggatg ctttgcggga
     1081 aatttccgga aaggaataat ttgggaaagg ggagaagggc ctttcatccg gaagatgagg
     1141 aggggagata tctgtattcg gtttgctgcg gttgatggcg ccaaggcgcg aaaatccggt
     1201 taatgcacgc aatccggaaa gccttcccgc cggaaaatgc cgcggacctg ttccgccagt
     1261 tttgcggacg cttccggggt gcccttcaag gtataatcca gcttcaattc atcccatttg
     1321 aatctcccca tctggtggaa agggagcacg tccacgcgtt ccacgttgcc caggagcgcc
     1381 gcatggcggg agagtccctc aatttcttcc agattatccg taacgccggg taccagcaca
     1441 tacctcagcc acacgggctt gcggagggag gccaggcgtt ccgcaaactc cagcgtaggg
     1501 cgcagttccc cgcctgtcag ggagcggtac cgttccggat tccatgcctt gatatccagc
     1561 aggaccaggt ccgtatccgc cagcatttct tcgcccgcat tcacgcccag atggccggag
     1621 gtgtccaggc aggtatgcag tcccagttcc cggcagcctt tcagcacggc gccggtaaaa
     1681 tcaggttgga agagggggtc cccgccggag agggtgacgc cgccgcccgc cgcctgcaaa
     1741 aaatcccggt aacgggctat ttccctgagg acatcacccg cgcttctgag ccggccccgc
     1801 cgaacgtagg aggtatccgg attgtggcag tagcggcagc gcaggctgca gccggacagg
     1861 aaaaggacaa agcggatgcc gggaccgtcc acggtgccgc aggattccac ggaatgaacc
     1921 aaaccggtga cggagctttc tccgtccgga tcatggtttt gtggaaaaac ctgcggcatc
     1981 cgtgtgtcgt ccgggttagc gggtgtggaa ggtgcggttg atgacctcct gctgctgttc
     2041 ccgggtcagc ttgatgaaat tcacggcata gccggaaacg cggatggtaa gctgcgggta
     2101 tttttccggg tgatccatgg cgtcgagaag cgtttcccgt tccagtacgt tcacattaat
     2161 gtggtgacct gtagcggcaa agtaggcgtc cagcagggag acgagcttga cgcggcgttc
     2221 cctttcctcc ttgcccaggg cctgcggaac gatggagaag gtgtaggaga tgccgtccag
     2281 ggagtcgtca taggacagct tggctacgga gagcatggac gccacggctc cgttcctgtc
     2341 ccttccgtgc atggggttgg cgccgggggc gaacggttcc ccggctcggc ggccgtccgg
     2401 cgtgttgccc gtctttttgc cgtacaccac gttggaggtg atcgtcagga tggactgggt
     2461 gggcagggaa ttgcggtaag tgtggagctt gcgcagtttt tccatgaaat tgctg
//
