LOCUS       c00176_gnlPro..         5484 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JBEJLKCB_176.
ACCESSION   c00176_gnlPro..
VERSION     c00176_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 10:58:18
            Original ID  :: gnl|Prokka|JBEJLKCB_176
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5484
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5484
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:3283]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..3283
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..5484
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5484
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             2..505
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg176_1"
                     /sec_met_domain="SucD_FumR (E-value: 6.5e-63, bitscore:
                     200.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="AGVVIHFVYAIILTLQNRKARGNDRYAINARPKGVEWASQNMFVL
                     GLIVILFMLLHFSQFWYKMMFAELIGHHEVALGSAMVSPQDGAAFINYYFQGNAVITVL
                     YLIWYVALWFHLTHGFWSAIQTIGWNNTIWMNRWECISKIVATVICGLFAIITIIFFLN
                     GVGA"
     CDS             554..2497
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 194.5; E-value:
                     1.1e-59)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg176_2"
                     /sec_met_domain="FAD_binding_2 (E-value: 1.1e-60, bitscore:
                     194.5, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 5e-39, bitscore:
                     122.0, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEINSKIPQGPLAEKWTNYKNHQKLVNPANKRRLDVIVVGTGLA
                     GASAAASLAEMGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKG
                     GDYRAREANVYRLAEVSNSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQT
                     GQQLLLGAYSALSRQIGLGKVKMYTRHEMLDVVKVDGRARGIIARNLITGKIERYAAHA
                     VVVATGGYVNTFFLSTNAMASNGSAAWQCYKKGAYFANPCMVQIHPTCVPVKGDFQSKL
                     TLMSESLRNDGRIWVPKKLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDV
                     ASRAAKERCDAGFGVNSTGLAVFLDFSDAINRLGKEVVKQKYGNLFDMYEEITNDNPYE
                     TPMMIYPALHYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYF
                     VLPYTIQNYLSDQIQVPRFSTDLPEFVEAEKAIKDRIQKLMNVKGKETVDTIHRKLGHI
                     MWEHIGMARDAKGLQEAIQMLKDLKKEFWSNVFIPGNADNLNTELEKALRVADFIEIGT
                     LMAHDALDRAESCGGHFRTEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNY
                     EFVVPQTRNYKK"
     CDS             2528..3283
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg176_3"
                     /sec_met_domain="Fer2_3 (E-value: 6.7e-27, bitscore: 82.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 1.3e-12, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.4e-06, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDISVTLKVWRQRGPKEKGQFETYKIEKINQSVSFLEMLDILN
                     EQLVNEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFHDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFTSVNTGGVPDANAIPIPKKDADLAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(3320..3430)
                     /locus_tag="ctg176_4"
                     /transl_table=11
                     /translation="MKKPRCKYTDFSIPVKGYNAKLCFTTYNKNKKAPFF"
     CDS             3706..4917
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Radical_SAM"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg176_5"
                     /sec_met_domain="Radical_SAM (E-value: 2.7e-16, bitscore:
                     49.1, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKKRTTSSFRAMSTERKLESIFLFVTGRCNAKCAMCFYAQEMDK
                     KQPDLTFDEIKKISETAGQFNRLWISGGEPTLREDLPEILELFYKNNHIKDVNIPTNGL
                     KPARIVEWVKRFRQNCPDCNINVSLSLDGFGTTHDTQRGVPGNFYKALETLRKIQENFG
                     DDGKVLKNIATVITKYNIDQVEDFMTWVYGRFRTSTHTIEAARGMTRDDGVKILTESTL
                     RKIQDDISPIYSAYADRMVADTTGLRKPITRFFYLGLIRTLYNVRASNIDHPTPWGMDC
                     TAGETTLVIDYDGRFRACELRKPLGNIKEYGCDISKVMNSEAMKQEIAAIGHGYKANCW
                     CTHGCWITSSVIFNPRKMIRSVYKGYRETKRLNHPLAINEQKLQTMEAKYHLDIERLRQ
                     LNIR"
     CDS             4951..5484
                     /locus_tag="ctg176_6"
                     /transl_table=11
                     /translation="MKILLVTRGSQGDVLPYLAIAAELERRGHKVTINLPQIFEETVKP
                     YGFKYVLQQFDDIGGMIDSAAQNSHKFRPFLKWMRNVIDKQFDQLIPLLKEHDILVSTN
                     SEFAVASIAEYCKKPLIRTAYAPFLPGKKIPPAVLPFPKPNPIITPAILWKLMNRMTNF
                     MVKDTINKNRAKYGL"
ORIGIN
        1 cgcaggcgtt gtgatccact tcgtgtacgc tatcattctt accttgcaaa accgtaaggc
       61 acgtggtaac gaccgttacg ctatcaacgc tcgtccgaaa ggcgtggaat gggcatctca
      121 gaacatgttc gttttgggtt tgatcgttat cctgttcatg ctgttgcact tcagccagtt
      181 ctggtataag atgatgttcg ccgaattgat cggtcatcac gaagtagcat tggggtcagc
      241 tatggtatct ccacaagacg gagccgcttt tatcaactat tacttccaag gtaatgccgt
      301 gatcaccgtt ctttatttga tctggtacgt ggctttgtgg ttccacttga ctcacgggtt
      361 ctggagcgct atccaaacaa ttggttggaa taatacgatc tggatgaacc gttgggaatg
      421 tatctctaag atcgtagcta cggttatttg cggacttttc gcgatcatta ctatcatctt
      481 cttcctaaac ggagtaggtg cttaattgca agctacaggt tttataatta agaacttttg
      541 aaattaaatc attatgtctg aaataaattc taaaatacct caaggcccgt tggctgagaa
      601 gtggactaat tataaaaatc accagaaatt ggtgaacccc gctaacaagc gtcgtttgga
      661 tgttatcgta gtcggtacag gtttggccgg agcttccgct gccgcatctt tggcagagat
      721 gggattcaac gtattgaact tctgtatcca agattctccc cgccgtgcgc actctatcgc
      781 cgctcaaggt ggtatcaacg ccgctaagaa ctaccagaat gatggtgact ctgtttaccg
      841 tttgttctac gatacgatca aaggtggtga ctaccgtgct cgtgaggcta acgtttatcg
      901 tttggctgag gtttctaact caatcatcga ccagtgcgtg gctcaaggcg ttcctttcgc
      961 tcgcgaatat ggtggtttgt tggataaccg ttcattcggt ggcgctcagg tatcgcgtac
     1021 gttctacgcc cgtggccaga cgggtcagca gcttttgtta ggcgcttatt cagcgttgag
     1081 ccgtcagatc ggtttgggta aggttaagat gtacactcgt catgagatgt tggatgtagt
     1141 taaggtagac ggtcgtgctc gtggtatcat cgcccgcaac ttgattacag gtaagatcga
     1201 gcgttacgcc gctcacgctg tagttgtagc tacgggtggt tacgttaata cgttcttctt
     1261 gtcaaccaac gctatggcat ctaacggttc cgccgcatgg caatgctaca agaaaggcgc
     1321 ttacttcgct aatccttgta tggttcagat ccacccgact tgcgtaccgg tgaaaggtga
     1381 cttccaatct aaattgactt tgatgtccga atccttgcgt aacgacggcc gtatctgggt
     1441 tccgaagaaa ctggaagacg ctaaagctct acaggccgga acgaagaaag gtaaagatat
     1501 tcccgaggca gaccgtgatt attatttgga gcgtcgttat ccggcattcg gaaacttggt
     1561 tcctcgcgac gtggcttctc gtgccgctaa agagcgttgc gacgcaggtt tcggcgtgaa
     1621 cagcacgggc ttggccgtat tcttggattt ctccgacgcg atcaatcgct tgggtaaaga
     1681 ggtcgttaag cagaaatacg gtaacttgtt cgatatgtac gaagagatca cgaacgataa
     1741 tccatacgag actccgatga tgatctaccc ggcattgcac tactcaatgg gtggattgtg
     1801 ggttgactat gagttgatga cctccattcc gggtctgttc gctatcggtg aggcaaactt
     1861 ctccgatcac ggagcgaacc gtttaggtgc ttccgctttg atgcagggat tggccgatgg
     1921 ttatttcgta ttaccataca cgatccagaa ctatttgtcc gaccaaatcc aagttcctcg
     1981 cttcagcacg gatctgccgg agttcgtcga ggcagaaaag gccatcaagg atcgtatcca
     2041 aaaattgatg aacgtcaagg gtaaagaaac cgtagacact atccaccgca aactaggtca
     2101 tatcatgtgg gaacatatcg gtatggcacg cgacgcaaaa ggtttgcaag aagctatcca
     2161 gatgctgaaa gacttgaaga aagagttctg gagcaacgta ttcatcccag gcaacgcaga
     2221 caacttgaat accgagttag agaaagcatt acgtgtagcc gactttatcg agatcggtac
     2281 attgatggca cacgatgctt tggaccgcgc tgagtcttgt ggtggtcact tccgtacgga
     2341 gcaccagaca gaagaaggtg aggccttgcg tcatgacgat aagttcatgt acgtatcttg
     2401 ctgggagtac caaggcgagg acaaagatcc tgtcatgttg aaagaggatt tgaactatga
     2461 gttcgttgtt cctcaaacac gtaactacaa gaagtaataa tcatcaatct cagaaaaaat
     2521 tagaatcatg gataaagata taagtgtaac acttaaggtt tggcgtcaga gaggtccgaa
     2581 ggagaaagga cagttcgaga catacaaaat tgagaagatc aatcagagtg tctctttctt
     2641 ggaaatgttg gatatcttga acgagcagtt ggtcaacgag ggtaaggaac cgatcgtatt
     2701 cgatcacgac tgccgcgagg gtatctgtgg catgtgttca ttgtatgtaa acggacaccc
     2761 gcacggaccg gcaactggag ctacgacttg ccagttatat atgcgtcgtt tccacgatgg
     2821 agagacgatc acgatcgaac cgtggcgttc cgcaggtttc cctgttatcc gcgacttgat
     2881 ggtagaccgc tacgcttacg acaaaatcat ccaagcgggt ggtttcacat ctgtcaacac
     2941 aggtggtgta cccgacgcta atgcgatacc tatcccaaag aaggacgcag acttagccat
     3001 ggacgctgcc gcttgtatcg gttgtggcgc ttgtgctgcc gcttgtaaga atggttccgc
     3061 tatgttattc gtatccgcta aggttagcca gctggctttg ttgcctcaag gtaaagtcga
     3121 ggctgctcgc cgtgccaaag ctatggtcgc taaaatggat gagcttggct tcggtaactg
     3181 tacgaacaca cgtgcttgcg agatggaatg tccgaagaat atctccgtta gcaacatcgc
     3241 tcgtttgaac cgcgagttca tttgcgctaa attacaagac taatatttga ttagtatatc
     3301 atggaaaagg cgtctttctc taaaagaaag gcgccttttt gtttttgtta tatgtcgtaa
     3361 aacataattt agcattatag cccttgacag gaattgaaaa gtccgtatat ttgcatcgtg
     3421 gttttttcat aatagtatta gatttaaggt taacaaagtt tggttagggc ttgtcgtgag
     3481 acaggcttta attgtttata gacctacctt ttaaacatgg accataaaat actgcaaata
     3541 gagattgttt agcgtcattt tatcgtcgat ctttgccttt aaagatcata tagtatatgc
     3601 taataagtat tttatttggc ttcttatgtt attttctccg ggtgttgatc aaacacaagg
     3661 aaaaaatcat gcgccagata agacaaatgg taaaacaaaa ataaaatgga caaaaaacga
     3721 accaccagtt ctttcagggc aatgagcacg gaaagaaaac tcgagtcgat cttccttttc
     3781 gtaacaggcc gatgcaacgc caaatgcgcg atgtgtttct atgcccaaga aatggataag
     3841 aagcaacccg acctcacttt cgatgaaatc aagaagatat cagaaaccgc cgggcaattc
     3901 aaccgattat ggatatccgg aggtgagcct accctgcgtg aggatctacc ggaaatattg
     3961 gagttattct acaaaaacaa ccatatcaaa gatgtgaaca tccctacgaa cggcttaaaa
     4021 ccagcccgga tcgtcgaatg ggtaaaacgg ttccgccaga actgcccgga ttgtaatatt
     4081 aacgtaagcc tttccttgga tggtttcggt acgacccacg atacacagag aggcgtaccg
     4141 ggaaacttct ataaggcctt ggaaaccctg cgtaagatac aggagaattt cggagacgac
     4201 ggcaaggtcc tgaaaaatat agcaaccgtt atcacgaaat acaatataga ccaggtagaa
     4261 gacttcatga cttgggtata cggacgtttc cgcacctcca cgcatacgat tgaggccgcc
     4321 agaggcatga cccgtgacga tggcgtgaaa atcctaaccg agtcaacgct acgcaaaata
     4381 caagacgaca tatcccctat ctattctgct tatgcggatc ggatggtagc ggataccacc
     4441 ggcttaagga aaccgatcac acgcttcttc tatctcggcc ttatacgtac cttatataat
     4501 gtgcgtgcca gcaacataga tcacccgacc ccttggggga tggattgtac ggccggagag
     4561 actaccttgg taatcgacta cgacggacgt tttcgtgcct gcgagctacg gaaaccgctc
     4621 ggaaatataa aagagtatgg ttgcgatata agtaaagtga tgaatagcga agcgatgaaa
     4681 caggaaatag ccgctatcgg acatgggtat aaagccaact gttggtgtac gcacggctgc
     4741 tggatcacct catccgtcat tttcaatcca cgtaagatga ttcgctccgt atataaagga
     4801 taccgggaaa cgaaacgatt gaatcatccc ctagccatca atgagcagaa actacaaacg
     4861 atggaagcca aataccatct ggacatcgag cgtttgcgtc aactgaatat ccggtgaaca
     4921 agaccctcat agcccataaa taaacgtagc atgaaaatac tattagtaac cagaggctca
     4981 caaggcgacg tccttcctta tttggccata gcggccgaat tggaaagacg tggccataag
     5041 gtgaccatca atctccctca aatattcgag gagaccgtta aaccctacgg atttaaatac
     5101 gtattgcagc agtttgacga tatcggcggc atgatcgata gcgcagcgca aaactcacat
     5161 aaattccggc cattccttaa atggatgagg aacgtgatcg ataaacagtt cgatcaattg
     5221 attcccctac taaaagagca tgatatttta gtatcgacca actcggaatt tgctgtcgca
     5281 agtatcgccg agtattgcaa gaagcccttg atacgtaccg cctacgcccc gttcttgccg
     5341 ggtaagaaga ttcccccggc cgtattgcca tttcccaaac ccaacccgat catcacccct
     5401 gccatcctat ggaagttgat gaaccggatg accaatttca tggtcaaaga cacgatcaac
     5461 aagaaccggg ctaaatacgg actc
//
