LOCUS       c00715_gnlPro..         2797 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MOKPMBAA_715.
ACCESSION   c00715_gnlPro..
VERSION     c00715_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 18:02:31
            Original ID  :: gnl|Prokka|MOKPMBAA_715
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2797
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..633)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 73; E-value:
                     9.5e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg715_1"
                     /sec_met_domain="Radical_SAM (E-value: 2.4e-30, bitscore:
                     93.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQE"
     protocluster    1..2797
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2797]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2797
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2797
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2797
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(647..2797)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg715_2"
                     /sec_met_domain="Gly_radical (E-value: 1.2e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1169.2, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="TPYYGDASFLEGPTERTKAVWNRCLEALAEERENNGVRSLDNVTV
                     STITSHKAGYIDKENELIVGLQTDELLKRAIKPFGGINVVSKACHENGVEVDDRVKDIF
                     THYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPDNYARGRIIGDYRRMALYGIDRLIEAK
                     KEDLHNLTGPMTEARIRLREEVAEQIKALKDMKVMGEYYGLDLSRPAYTAQEAVQWVYM
                     AYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSKGTITESFAQELIDQFVIKLRMVRHLR
                     MQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKTSFRFLQTLYNLGPSPEPNLTVLWSPE
                     LPEGFKEFCAKVSIDTSSIQYENDDLMREVRQSDDYGIACCVSYQEIGKQIQFFGARCN
                     LAKALLLAINGGRCENTGTVMVKNIPVLTSDTLKFEEVMDNYKKVLTEIARVYNEAMNI
                     IHYMHDKYYYEKAQMALVDTNPRINLAYGVAGLSIALDSLSAIKYAKVTARRNDIGLTE
                     GFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEELKKLPVYKNARPTLSLLTITSNVMYGK
                     KTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDSQDGISNTFSIVPKS
                     LGATDEDRIENLVTMMDGYFTKGAHHLNVNVLNRDMLYDAMEHPENYPQLTIRVSGYAV
                     NFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 ttcctgtccc atcgcttcgt acttatgtac gcccagtgta tggtaaggca gtatttccac
       61 ccgttggatc atcttgtatt ttccgagtgc ttctcccagt gcgcgtatgt cttcttcaaa
      121 atcgctgtat ccgggcacta gcacgtagcg tagccagaaa ggtttctcct gttcttccag
      181 ccaggcggcc gtgcggatgg tttgctcgtt gcttcttccg gtgagcgttt gatggcggtt
      241 agggttgaat tcctttatat ccaataacac aagatcggtc agtctgaata attcttctac
      301 gtcttcgttc caaagtcctc cgttgctgtc cagacagaca tggatgcctt tttctttcag
      361 ttcgcggacc agcgggacga gtgctttcgc ctgaaacgtc ggttcgcctc cggagaaagt
      421 gattcctcct cgtttcccga agaaggggcg ctggctcatt gccatgcgga cgatttcttc
      481 cggtggggta ggcgtgcctc cctttcctgc tatcgtatcc ggattggcgc aatagagaca
      541 acggaagttg catccttgaa gaaaaacgac gagccgtaag cccggcccgt cgaatgttcc
      601 catactttcg tatgaatgta cgtttatcat catatagagt aatagattac atacgttcgt
      661 ggaagctacg gctgataact tccagttggt gttcgcggct cagcttcacg aagtttacgg
      721 catagccgga aacgcggata gtcagctgcg gatagttttc cggatgctcc atggcatcgt
      781 acagcatatc acggttcagt acgtttacgt tcaggtggtg ggcacctttg gtgaagtaac
      841 cgtccatcat cgttacgagg ttctcgatac ggtcttcgtc cgtagcaccc agtgatttcg
      901 gaacgataga gaaggtgttg ctgataccgt cctgtgagtc acggtaacga agtttcgcta
      961 cagaactcag agaagcgatc gcaccattct tgtcgcgtcc gtgcatcggg ttggcacccg
     1021 gagcgaaggc aacacctttg gcacgtccgt cgggagtagc accggtcttt ttgccgtaca
     1081 tcacattgga agtgatagtc agcaaggaga gggtaggacg ggcattcttg taaacaggca
     1141 atttcttcaa ttcttcgctg aagaagtata ccaggtctac gccaaggtgg tctaccttgt
     1201 cgttgtcgtt accgaagcaa gggaattctc cttcgatgtc gaagccttct gtcagaccga
     1261 tatcattgcg gcgggcagtt actttcgcat atttgatggc agacagtgaa tcgagggcga
     1321 tggaaagtcc ggctacaccg taagctaggt tgatgcgcgg gttcgtatct acaagagcca
     1381 tttgggcctt ctcgtagtaa tacttgtcgt gcatatagtg gatgatgttc atcgcttcgt
     1441 tatatacgcg ggcgatttcg gtcagcactt ttttgtagtt gtccattact tcttcgaact
     1501 tcagcgtgtc actggtcagt acggggatgt tcttcaccat gactgtaccg gtattctcgc
     1561 aacgtccgcc gttgatggca agcaacaggg ctttggccag gttgcaacgg gcaccgaaga
     1621 actgaatctg cttacctatt tcctggtatg atacgcagca ggcgattccg tagtcgtcgg
     1681 actgacgtac ttcgcgcatc aggtcgtcat tttcgtactg gatagaagaa gtgtcgatag
     1741 aaacctttgc acagaattct ttgaatcctt ccggaagttc cgggctccag agtacggtca
     1801 ggttcggttc cggtgaaggg ccgaggttgt acaatgtctg caggaaacgg aaggaggtct
     1861 ttgtcacttt ggtacgtccg tcgttgagac gtccgcccag agattcggtt acccaggtcg
     1921 ggtcgcctgc aaagatgtcg ttgtatgatt gcatacgcag gtggcggacc atacgcagtt
     1981 tgatgacaaa ctggtcgatc agctcttgcg cgaatgattc ggtgatggtt cctttgctca
     2041 gttcatattc cagatagata tcgaggaaag aagaaacgtt acccagtgac atggcggcac
     2101 cgtcttgttc tttgacggcg gcaaggtaag ccatatatac ccactgtacg gcttcttgtg
     2161 ccgtgtaagc gggacggctc aggtcgagac cgtagtattc gcccatcact ttcatgtctt
     2221 tcagtgcctt gatctgttct gccacttctt cgcgcaggcg gatgcgggcc tctgtcatcg
     2281 gaccggtgag gttgtgcaaa tcttccttct ttgcttcgat cagacggtcg atgccgtaaa
     2341 gtgccatacg gcggtagtca ccgatgatgc gtccgcgtgc ataattgtcg ggaagtccgg
     2401 taaggaatcc cagcgaacgg aaggaacgga tttcttccgt atatacgtcg aatactccgt
     2461 cgttgtgcgt cttgcggtag tgagtgaaaa tatcttttac gcggtcgtcc acttccacgc
     2521 cgttttcgtg acaggctttg ctgactacgt tgatacctcc gaaaggcttg atggcacgtt
     2581 tcagaagttc gtctgtctgc aggccgacga tcagttcgtt ttccttgtcg atatatccgg
     2641 ccttgtggga agtgatggtt gatacggtaa cattatctaa tgagcggaca ccgttgtttt
     2701 ctctttcttc tgccagtgct tcgaggcagc ggttccatac ggctttggta cgttctgtag
     2761 gtccttcgag gaatgaagca tctccgtaat acggagt
//
