LOCUS       c00685_gnlPro..         2024 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IBMBAINL_685.
ACCESSION   c00685_gnlPro..
VERSION     c00685_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 11:15:36
            Original ID  :: gnl|Prokka|IBMBAINL_685
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2024
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2024
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[304:2023]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      305..2023
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..2024
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2024
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(2..106)
                     /locus_tag="ctg685_1"
                     /transl_table=11
                     /translation="MKTPQEIAEMISKRFYPLPDADLDSLASIIESSKA"
     CDS             complement(305..1039)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg685_2"
                     /sec_met_domain="Radical_SAM (E-value: 3.8e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             complement(1040..2023)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg685_3"
                     /sec_met_domain="Gly_radical (E-value: 2.8e-47, bitscore:
                     147.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 8.2e-78, bitscore:
                     249.9, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRGEYLEYD
                     EVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGIAGLSIV
                     ADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFMEYLRQH
                     ETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGAIAALAS
                     VAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNVNVFDKD
                     LLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
ORIGIN
        1 cagccttact tgattctatg atcgaggcca gagaatccaa atccgcatcg ggtaatggat
       61 aaaaacgttt ggatatcatt tcggctattt cttgtggtgt cttcatacta ttcatcattt
      121 tctgatctat atcatttttt atctccttac aaaaatagat atttgttgca tataacaaaa
      181 caaacacata ttaaatagtc aaggttagtc taggcaaaaa attattacta tcgtttaaaa
      241 gctaatcgga ttagattgtt ttccctgatt agctgttgca taatcgcaag gcttgatcga
      301 gagatcaggc cttgttattt tctggtttgt agcgagagaa tatcgctttc gcattatcta
      361 aacgttcttt tgataagggt tccaccccct ttaacggata atcgagacct aattgctcat
      421 atttataggt tcccatcgta tgataaggca ataattccac tttgcgaacc actttatatg
      481 aactgacata ccgagctaac gcctccagcc actcgtcatt atccgtatat ccggggacaa
      541 taacatggcg aatccaagta tctatgccac gtctctccaa ttcatccaag aacagaagcg
      601 tattatcctg cttgacaccc gccagtaacg gatgtaaatc cgggtttaag gttttgatgt
      661 ccaataacac taagtccgca taatccaaga cttcccaagc ttttgaggta catacgaaac
      721 cagatgtgtc caaagctgtg tgaagacctt gctcttggca caaacggaaa aactctttca
      781 gaaactcagg ttgcaataaa ggttccccac cggtaacagt caccccccca cgggcgataa
      841 aactcttata ccgtaagact tccgtcaata gctcgccggg agtcatctga tatttcacct
      901 tccctttcgg gttccaagta tccgggttat ggcaatacag gcaacgcaac gggcagccct
      961 gcataaaaac gacaaagcga attccgggac catctacggt cccgaaactc tctaatgaat
     1021 gtatcttacc ttttatcact tacagtgaat ggttaatggt tcttgaaata acatccaact
     1081 gttgttcacg agtcagtttc acgaagttta ccgcatatcc ggatacacgg atcgtaagtt
     1141 gcggatattt ctcaggatgt tccatagcgt cgatcaacaa atccttatca aatacgttaa
     1201 cattcagatg ctgacctcca tccggagtaa aatagccatc caataagcta accagattat
     1261 tgatctgcac ttcacgctcc tttcccaacg tagccggaga aacagcgaaa gtataagaga
     1321 taccgtcgtg cgcatgctgg aatggaagct tcgctaccga agctagcgca gcgatagcac
     1381 ctttcgtatc acgaccgttc atagggttcg cgcccggagc gaaaggagtt ccaccgatac
     1441 ggccatccgg tgtcgtacca gtcttcttac catataccac attagaggtg atcgtaagga
     1501 tggactgcgt aggtttcgca tggcgatagg tctcgtgctg acgcaaatat tccatgaact
     1561 tctcggtgat ctctacagcg atactatccg tacgatcgtc gttattaccg aaaggaacat
     1621 attcgccctc acgttcgaaa tcaaccgcca agccacgctc gtcacggatc acacgtactt
     1681 tcgtatcacg gatagcagcc aacgaatcgg caacgataga aagaccggcg atacctgtcg
     1741 cacggatacg ctctacatct ccatcgtgaa gcgacatctc gaatgcctca tatgcgtatt
     1801 tatcatgcat ataatggatt atcttcaagg cgttcacata cactttcgcc aaccagcgca
     1861 tcatttgctc atacttagcc atcacctcat cgtattccag atactcgccg cgaaccggct
     1921 cgaacatcgg ggctacctgt acgccgctgc gctcatcccg tcctccgttg atcgcatata
     1981 ataaacattt cgccaaattc gcacgggctc cgaagaactg catc
//
