LOCUS       c00216_gnlPro..         7338 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FBACGABO_216.
ACCESSION   c00216_gnlPro..
VERSION     c00216_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 17:32:43
            Original ID  :: gnl|Prokka|FBACGABO_216
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7338
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..177
                     /locus_tag="ctg216_1"
                     /transl_table=11
                     /translation="AERQGFEPRVPRGTTVFKTAAIDHSATSPKLPYQKCFSLKAVQRY
                     EDILNLQEFSLKI"
     protocluster    1..7338
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[326:3297]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      327..3297
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7338
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7338
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             327..2555
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg216_2"
                     /sec_met_domain="Gly_radical (E-value: 5.6e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1173.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERENNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     KFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLN
                     RDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             2569..3297
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.5;
                     E-value: 1.4e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg216_3"
                     /sec_met_domain="Radical_SAM (E-value: 1.7e-29, bitscore:
                     92.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(3290..3739)
                     /locus_tag="ctg216_4"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDKDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(3726..3893)
                     /locus_tag="ctg216_5"
                     /transl_table=11
                     /translation="MKEIVSRILDVIMYLIPFFGKRKRDRVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             complement(3953..4357)
                     /locus_tag="ctg216_6"
                     /transl_table=11
                     /translation="LDFEELCEAITSRSTCTETDVRAAISGILYEVKRALKAGRIARLG
                     DLGSLQIGLNSEGAASVKEFSGSMIKGAHLIFRPGKTLAELMKILSYQQVQTRAVAQAG
                     AGDGGGEDPDKNPDSGGDGSGDEEAPDPTV"
     CDS             4617..4847
                     /locus_tag="ctg216_7"
                     /transl_table=11
                     /translation="MNYKEDSFLIRTYTKAELAHLYNPHVCLKVALQILRRWIIYNLPL
                     LHELEQEGYRARNRLLSPKQVATIIHFLGEP"
     CDS             4934..7078
                     /locus_tag="ctg216_8"
                     /transl_table=11
                     /translation="MKHFTIFQGFYYAIAEMTEEEIVSTIGSFTYREKVEEIRRIFAEQ
                     GEKAANEKKKELPAIAFSASYRGRRTKVNLVKYLGHIVIDIDHLSKEELARILPIIKRC
                     DYTRIAFISPKGMGVKIIVRACHPDETLPETLQEIEDFHHAAYTRLVSFYTELCQIEID
                     TSGQDVARTCLFSYDPEIYFNPNADAFLVDQPQASYKVSNRKNLSGSKQQTPPDGPPTN
                     EDTALNAHSANASLVLTLTYYHNKSEKYIVGNRNNYLHHLSCTFNRYGIPQEEASAFIK
                     SQFTDFPADETASLINSAYAHTDEFNTCKLNGTQKRILRIEQYISEHYETRYNEVLHIM
                     EYRRRHPDTEDRKSTRLNSSHXXXXRILDEMMENSIWIEINELGYACTVKTIENLIYSD
                     FSQSYHPIREYFELLPEWDGTDYIRILADSVQTSHQEFWAECLERYLVAMCAAATQENI
                     VNHTVLLLCSEIQNIGKTTFINNLLPPELRAYLSTGLINSNNKDDLAKITQAMLINLDE
                     FEGMSGRELNAFKDLVTRKVISFRLPYARRSQNFPHTASFAGTCNYQEVLHDTTGNRRF
                     LCFHPYSIQFITINYAQLYAQIKYLLNKPGYQYWFTQADNERLEENNEAFIFHSPEEEM
                     VLTHIRKPERFEKVYYLTVSEIAELIRERTGYQYSIGSKIQLGKVMTKHHFESKKGNNG
                     RRYAVFIIDIEQVKSNRLYE"
     CDS             complement(7148..7336)
                     /locus_tag="ctg216_9"
                     /transl_table=11
                     /translation="TGQIIEDARKKAKITQAELARRIGSDRSYISRVESGQTEPKVSTF
                     YRIMNALGCKIEFSMIL"
ORIGIN
        1 gcggagagac agggattcga accccgggta cctcgcggta caacggtttt caagaccgcc
       61 gcaatcgacc actctgccac ctctccaaaa cttccttatc agaagtgctt ttcgttaaaa
      121 gcggtgcaaa ggtacgaaga tattttaaac ttgcaagagt tttcactaaa aatttaatct
      181 aatcttttcc taaggatagc aattaatagg tattttatgt tgaaaagcat aaaatagagc
      241 atatttatgc tgattatatg aaaaatgatt gtaactttgt gcgctctaaa gaataggata
      301 actacaacct ctaattttat cattttatgg aattaaacaa gatctttaaa gacggtcttt
      361 ggagcagcga aatcaacgtc agagatttcg taagtcataa catcactccg tattacggag
      421 atgcttcatt cctcgaagga cctacagaac gtaccaaagc cgtatggaac cgctgcctcg
      481 aagcactggc agaagaaaga gaaaacaacg gtgtccgctc attagataat gttaccgtat
      541 caaccatcac ttcccacaag gccggatata tcgacaagga aaacgaactg atcgtcggcc
      601 tgcagacaga cgaacttctg aaacgtgcca tcaagccttt cggaggtatc aacgtagtca
      661 gcaaagcctg tcacgaaaac ggcgtggaag tggacgaccg cgtaaaagat attttcactc
      721 actaccgcaa gacgcacaac gacggagtat tcgacgtata tacggaagaa atccgttcct
      781 tccgttcgct gggattcctt accggacttc ccgacaatta tgcacgcgga cgcatcatcg
      841 gtgactaccg ccgtatggca ctttacggca tcgaccgtct gatcgaagca aagaaggaag
      901 atttgcacaa cctcaccggt ccgatgacag aggcccgcat ccgcctgcgc gaagaagtgg
      961 cagaacagat caaggcactg aaagacatga aagtgatggg cgaatactac ggtctcgacc
     1021 tgagccgtcc cgcttacacg gcacaagaag ccgtacagtg ggtatatatg gcttaccttg
     1081 ccgccgtcaa agaacaagac ggtgccgcca tgtcactggg taacgtttct tctttcctcg
     1141 atatctatct ggaatatgaa ctgagcaaag gaaccatcac cgaatcattc gcgcaagagc
     1201 tgatcgacca gtttgtcatc aaactgcgta tggtccgcca cctgcgtatg caatcataca
     1261 acgacatctt tgcaggcgac ccgacctggg taaccgaatc tctgggcgga cgtctcaacg
     1321 acggacgtac caaagtgaca aagacctcct tccgtttcct gcagacattg tacaacctcg
     1381 gcccttcacc ggaaccgaac ctgaccgtac tctggagccc ggaacttccg gaaggattca
     1441 aagaattctg tgcaaaggtt tctatcgaca cttcttctat ccagtacgaa aatgacgacc
     1501 tgatgcgcga agtacgtcag tccgacgact acggaatcgc ctgctgcgta tcataccagg
     1561 aaataggtaa gcagattcag ttcttcggtg cccgttgcaa cctggccaaa gccctgttgc
     1621 ttgccatcaa cggcggacgt tgcgagaata ccggtacagt catggtgaag aacatccccg
     1681 tactgaccag tgacacgctg aagttcgaag aagtaatgga caactacaaa aaagtgctga
     1741 ccgaaatcgc ccgcgtatat aacgaagcga tgaacatcat ccactatatg cacgacaagt
     1801 attactacga gaaggcccaa atggctcttg tagatacgaa cccgcgcatc aacctagctt
     1861 acggtgtagc cggactttcc atcgccctcg attcactgtc tgccatcaaa tatgcgaaag
     1921 taactgcccg ccgcaatgat atcggtctga cagaaggctt cgacatcgaa ggagaattcc
     1981 cttgcttcgg taacgacaac gacaaggtag accaccttgg cgtagacctg gtatacttct
     2041 tcagcgaaga attgaagaaa ttgcctgttt acaagaatgc ccgtcctacc ctctccttgc
     2101 tgactatcac ttccaatgtg atgtacggca aaaagaccgg tgctactccc gacggacgtg
     2161 ccaaaggtgt tgccttcgct ccgggtgcca acccgatgca cggacgcgac aagaatggtg
     2221 cgatcgcttc tctgagttct gtagcgaaac ttcgttaccg tgactcacag gacggtatca
     2281 gcaacacctt ctctatcgtt ccgaaatcac tgggtgctac ggacgaagac cgtatcgaga
     2341 acctcgtaac gatgatggac ggttacttca ccaaaggtgc ccaccacctg aacgtaaacg
     2401 tactgaaccg tgatatgctg tacgatgcca tggagcatcc ggaaaactat ccgcagctga
     2461 ctatccgcgt ttccggctat gccgtaaact tcgtgaagct gagccgcgaa caccaactgg
     2521 aagttatcag ccgtagcttc cacgaacgta tgtaatctat tactctatat gatgataaac
     2581 gtacattcat acgaaagtat gggaacattc gacgggccgg gcttacggct cgtcgttttt
     2641 cttcaaggat gcaacttccg ttgtctctat tgcgccaatc cggatacgat agcaggaaag
     2701 ggaggcacgc ctaccccacc ggaagaaatc gtccgcatgg caatgagcca gcgccccttc
     2761 ttcgggaaac gaggaggaat cactttctcc ggaggcgaac cgacgtttca ggcgaaagca
     2821 ctcgtcccgc tggtccgcga actgaaagaa aaaggcatcc atgtctgtct ggacagcaac
     2881 ggaggacttt ggaacgaaga cgtagaagaa ttattcagac tgaccgatct tgtgttattg
     2941 gatataaagg aattcaaccc taaccgccat caaacgctca ccggaagaag caacgagcaa
     3001 accatccgca cggccgcctg gctggaagaa caggagaaac ctttctggct acgctacgtg
     3061 ctagtgcccg gatacagcga ttttgaagaa gacatacgcg cactgggaga agcactcgga
     3121 aaatacaaga tgatccaacg ggtggaaata ctgccttacc atacactggg cgtacataag
     3181 tacgaagcga tgggacagga atacaagatg aaaggggtaa aagaaaatac ccccgaacag
     3241 cttgaaaaag ctgcggaggt attcaaagag tatttcacta cggtggtagt taattaatgg
     3301 agaattctcc aatgacctca aagcaagggc attctttaat ccgttcccaa ggatctacga
     3361 tcccattata gttggtgtcg ggacttaaat cccgatgccc tactacttta gcttccggat
     3421 aacgctgcaa caattcgtgt accaagcgat gtagcgcctc cttctgacgg ggtgtacgcg
     3481 tatccgcagc ttttccattc tcatccagac cgccctcata acatacacct aagctgatcg
     3541 cattatagcc acgtgcatga gctccgaccg tatctttatc ccgcatcggc tcaatctcgc
     3601 ccgacttgcg tatataataa tgataccccc agcacgaaaa gccacgataa cggtgagccg
     3661 tatccacatc ctttgccgta aagtcgcaat cgacacgggt ggcggaacaa tggatcacaa
     3721 tcagactaat ctttctcatg ttccttttcc ctttccgtcg ttttcacttt gcacacctcc
     3781 ttacaagtag tatgatagcg cacttctctc accaccctgt cccgctttct ctttccgaag
     3841 aaaggaatca gatacatgat cacatctaaa atccgactta ctatttcttt catcttatca
     3901 aaatattaat ggaagttatt ctaattcgtt attaccttga cccgtctgcc aattataccg
     3961 tagggtccgg agcctcttca tcaccggagc catcaccacc ggaatccgga ttcttgtcag
     4021 ggtcttctcc cccgccatcg cctgcacccg cctgcgccac cgcacgagtc tgcacttgct
     4081 gataactcag aatcttcatc aactccgcca gcgtcttacc ggggcggaaa ataagatgcg
     4141 cccccttaat cattgaaccg gaaaattcct tgaccgatgc cgcaccttca ctgttgagtc
     4201 ctatctgcaa actgcccaaa tcgcccagtc tcgcaattct tcctgccttc aacgcacgct
     4261 tcacttcgta aagaataccc gaaatagccg cacgtacatc cgtttccgta caagtagaac
     4321 gactggtgat cgcttcgcac aactcttcaa agtccaactc tcccgacgcc tgcgcctgtg
     4381 cataatactt tgccggttcg ctcttttttg caggatnnnn nnnnnnaaaa taattttaaa
     4441 gtttataata cataggatgc atccccttct tgttaacagc acaaagataa ggatagccgg
     4501 acaagccata ttccccaacg aagtttcttg attcccaacg aataaaaacg aagtcaaacc
     4561 tttgaataac aaggaatttt atgtaacttt atagcataaa acaagaagaa aacatcatga
     4621 attacaaaga agattctttc cttatccgca cctataccaa agcggaactg gctcaccttt
     4681 acaatcccca tgtctgcctc aaagttgctt tacaaatact tcgtcgttgg attatttaca
     4741 acctccctct cctgcacgaa ctcgaacagg aaggctaccg cgcacgcaac cgtctccttt
     4801 cgccgaaaca agtggcaact atcatacact ttttgggcga accgtagtaa cttattgcca
     4861 ttttatacaa agttccttta cacatttccg caaagaagtt cctttgcatc gacaaacaat
     4921 aatcaaaata aaaatgaaac atttcaccat atttcaaggt ttctactacg caatagcaga
     4981 gatgaccgaa gaagaaatag tgtccactat cggcagtttt acctaccggg aaaaagtcga
     5041 agaaatacgc cgcatctttg cagagcaagg agaaaaggct gccaatgaga agaaaaaaga
     5101 attgccggca atcgccttct ccgccagcta ccgaggaagg cgtaccaaag tgaacctcgt
     5161 caaatatctg ggacacatcg tgatagacat cgaccacctc agcaaagaag aattggcacg
     5221 catcctccct atcatcaaga gatgcgatta cacccgcatc gcctttatca gccccaaagg
     5281 gatgggcgtg aaaatcatag tacgcgcatg ccatccggat gagacattgc ccgaaaccct
     5341 tcaggaaata gaagacttcc atcatgcagc ttacaccaga ctcgtttctt tttacaccga
     5401 actctgtcag atagaaatcg acacctccgg tcaggatgtg gcacgcactt gtctgttctc
     5461 ttacgatccg gagatttatt tcaatccgaa cgctgacgct tttcttgtag atcaacctca
     5521 ggcatcctac aaggtttcaa atcgtaaaaa tctctccggc agtaaacaac agactcctcc
     5581 ggacggcccc ccgacgaatg aagacacagc gctcaacgcc cattccgcca acgcttctct
     5641 ggtgctgact ttaacctact atcacaacaa gtccgaaaaa tacatagtgg gcaaccggaa
     5701 caattatctg caccatctct catgtacctt caatcgctac ggcattccgc aggaggaagc
     5761 atccgccttt atcaaaagcc agtttacaga ctttccggcg gacgagacag cctctttgat
     5821 caatagcgcc tacgctcata ccgacgaatt taatacctgt aagctaaatg gcacgcagaa
     5881 acgaatatta cggatagagc aatatatcag cgagcactac gaaacacgct acaacgaagt
     5941 gctgcatatc atggaatatc gcagaaggca tcccgacacc gaagatcgga agagcacacg
     6001 tctgaactcc agtcacannn nnnnnnnccg catcttggat gagatgatgg aaaacagtat
     6061 ctggatagaa atcaacgaac tgggatatgc ctgcactgtc aaaacaatcg aaaatctgat
     6121 ttactccgat ttcagccagt cgtatcatcc cattcgcgag tattttgaat tactgcccga
     6181 atgggacgga accgactata tccgtatcct ggcggatagt gtccagacga gtcatcagga
     6241 gttttgggcg gaatgcctgg aacgttatct ggtggcgatg tgcgcagcgg ctactcaaga
     6301 gaatattgtc aatcacacgg tacttctgct atgcagcgag atacagaaca taggaaagac
     6361 tacctttatc aataatctcc tgcctcccga actgcgtgct tatctctcta ccggactaat
     6421 caattcgaac aataaagatg atttagccaa aataacgcag gccatgctga tcaatctcga
     6481 cgagtttgaa gggatgagcg gtcgggaact gaatgcattc aaagatttgg taacgcgcaa
     6541 agtgatcagt ttccgtctgc cttatgcacg ccgttcgcaa aacttccctc atacggcctc
     6601 ttttgcagga acctgcaatt atcaggaagt gcttcacgac acgacaggca accggcgttt
     6661 cctttgtttt catccttatt ccatccaatt tattaccatc aattatgcgc agctctatgc
     6721 tcagatcaaa tatctgctga ataagccggg atatcaatac tggttcacgc aagcggacaa
     6781 tgaacggctt gaagagaata acgaggcttt catctttcat tcaccggaag aagaaatggt
     6841 tcttacgcat atccgcaagc cggaacgttt cgagaaggtt tattatctga ctgtcagtga
     6901 gattgcggaa ctaatacgtg agcgtaccgg ttatcagtat tccatcggat caaaaataca
     6961 gttggggaag gtgatgacta agcaccattt tgaatctaaa aaaggaaata acgggcgccg
     7021 atatgccgta tttattatag atatcgagca ggtaaaatca aacaggctgt atgaatagaa
     7081 gcgggaaagc cttgtgaata tagcaaaatg cagagtcatc ttgacagata ctctgcattt
     7141 attgttttta caaaatcatt gaaaattcga ttttacagcc taacgcattc attatacgat
     7201 agaaagtaga tactttaggc tctgtctgac cactttctac tcttgaaata taagaacggt
     7261 cagagccgat acgccgggct aattcagcct gagtgatttt agcctttttt cttgcgtcct
     7321 cgataatctg tcctgtat
//
