LOCUS       c00320_gnlPro..         3673 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OOGJOHKA_320.
ACCESSION   c00320_gnlPro..
VERSION     c00320_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 13:54:59
            Original ID  :: gnl|Prokka|OOGJOHKA_320
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3673
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..723)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.5e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg320_1"
                     /sec_met_domain="Radical_SAM (E-value: 3.7e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPEN"
     protocluster    1..3673
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:2964]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..2964
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3673
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3673
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(724..2964)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg320_2"
                     /sec_met_domain="Gly_radical (E-value: 1.2e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 9.2e-283, bitscore:
                     926.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             complement(3135..3671)
                     /locus_tag="ctg320_3"
                     /transl_table=11
                     /translation="IIQSNNELLLRLINDILDLSKIESGIIERRKENFNLAKVCNELFV
                     TIQAKMTNPNVELRLDGPNSECWVLLDRNRLKQVWMNFLTNAVKCTKSGYIKMGYGIER
                     EGLRIYVEDTGIGIPDDLHDKVFTRFEKLNEFSQGTGLGLTISRAIVEAAGGEVGFTSK
                     LGVGSTFWAWLPLRP"
ORIGIN
        1 attttctggt ttgtagcgag agaatatcgc tttcgcatta tctaaacgtt cttttgataa
       61 gggttccacc ccctttaacg gataatcgag gcctaattgc tcatatttat aggttcccat
      121 cgtatgataa ggcaataatt ccactttgcg aaccacttta tatgaactga cataccgagc
      181 taacgcctcc agccactcgt cattatccgt atatccgggg acaataacat ggcgaatcca
      241 agtatctatg ccacgtctct ccaattcatc caagaacaga agcgtattat cctgcttgac
      301 acccgccagt aacggatgta aatccgggtt taaggttttg atgtccaata acactaagtc
      361 cgcataatcc aagacttccc aagcttttga ggtacatacg aaaccagatg tgtccaaagc
      421 tgtgtgaaga ccttgctctt ggcacaaacg gaaaaactct ttcagaaact caggttgcaa
      481 taaaggttcc ccaccggtaa cagtcacccc cccacgggcg ataaaactct tataccgtaa
      541 gacttccgtc aatagctcgc cgggagtcat ctgatatttc accttccctt tcgggttcca
      601 agtatccggg ttatggcaat acaggcaacg caacgggcag ccctgcataa aaacgacaaa
      661 gcgaattccg ggaccatcta cggtcccgaa actctctaat gaatgtatct taccttttat
      721 cacttacagt gaatggttaa tggttcttga aataacatcc aactgttgtt cacgagtcag
      781 tttcacgaag tttaccgcat atccggatac acggatcgta agttgcggat atttctcggg
      841 atgctccata gcgtcgatca acaaatcctt atcaaatacg ttaacattca gatgctgacc
      901 tccatccgga gtaaaatagc catccaataa gctaaccaga ttattgatct gcacttcacg
      961 ctcctttccc aacgtagccg gagaaacagc gaaagtataa gagatgccgt catgcgcatg
     1021 ctggaatgga agcttcgcta ccgaagctag cgcagcgata gcacctttcg tatcacgacc
     1081 gttcataggg ttcgcacccg gagcgaaagg agttccaccg atacggccat ccggtgtcgt
     1141 accagtcttc ttaccatata ccacattaga ggtgatcgta aggatggact gcgtaggttt
     1201 cgcatggcga taggtctcgt gctgacgcaa atattccatg aacttctcgg tgatctctac
     1261 agcgatacta tccgtacgat catcgttatt accgaaagga acatattcgc cctcacgttc
     1321 gaaatcaacc gccaagccac gctcgtcacg gatcacacgt actttcgtat cacggatagc
     1381 agccaacgaa tcggcaacga tagaaagacc ggcgatacct gtcgcacgga tacgctctac
     1441 atccccatcg tgaagcgaca tctcgaatgc ctcatacgcg tatttatcat gcatataatg
     1501 gattatcttc aaggcgttca catacacttt cgccaaccag cgcatcattt gctcatactt
     1561 agccatcacc tcatcgtatt ccagatactc gccgcgaacc ggctcgaaca tcggggctac
     1621 ctgtacgccg ctgcgctcat cccgtcctcc gttgatcgca tataataaac atttcgccaa
     1681 attcgcacgg gctccgaaga actgcatctg cttaccgatc ttcataggag aaacgcaaca
     1741 agcgatacca taatcatctc cataatccgg acgcatcaaa tcgtcgttct catattggat
     1801 agccgacgta tcgatagaga ccttggcaca gaaacgtttc cagttctcgg gagcgttctt
     1861 aaaccacaga accgtcaaat tgggttctgg tgccggacct agattatata atgtatggag
     1921 ataacgatag gaagttcttg ttaccaaaga acggccatcc actccttggc cgcccaagga
     1981 ctctgttacc caaaccggat caccggagaa caaatcattg tattccggcg tacgtaagaa
     2041 acgaacgata cgcaacttga tgatcagctg gtctaccaat tcttgcgcct cctcttcggt
     2101 caacttaccc tcacggatgt ctttctcgat ataaatatcc aaaaacgtag aagtacgtcc
     2161 gatagacatc gccgcgccat cttggtcttt taccgccgcc agataagcta aatacacaaa
     2221 ctgaaccgcc tcacgagcgt tttgtgccgg acgagtcaca tcaaagccat agctagcgca
     2281 cattttctcg aacgctttca aagccttgat ctgttcggaa atctcctcac ggctctggat
     2341 gatctcttcc gtgatctctt ggatatccaa acgattcaag aaacgctttt tctcctcgat
     2401 caagattgcc gtaccataca gagcgacacg acgatagtca ccaataatac gtcctctacc
     2461 atacgcgtcg ggcagaccgg tgataatagc cgaacgacgt gcggcaatca tctcttccgt
     2521 atatgcggag aaaacgccct cgttatgcgt cttacggtac ttcgtgaaga tctctttcgt
     2581 catcgggtcc aaatgataac cataagcttc caagctattc tcgaccatac ggataccgcc
     2641 tttcgggaag ataccacgtt tcagcggagc atcggtttgt aacccaacga tcacctcatt
     2701 ctccttatca atatagcccg ggccatatac atcgatccct tgaggtaatt ttgtctcggc
     2761 gtcataaact cccttttccc tctcgacctt aaacatctcg gtcagcttgt tccagacctt
     2821 acgggtcttc tcggaagaag cgactaaaaa ggaatcatcc ccttcataag gcgtatagtt
     2881 actctggata aaatctctta cattgatctc tcttttccag gtctcaccct taaagtgatc
     2941 ccagcactca ttctcaaatt tcataaacaa tcttatctaa taccacattt ttaccgccgc
     3001 aaaggtagga gaattatcgt catatttctg tcacatatgc tacaccagtt ctaaaatccc
     3061 tataaattgc gataaaaaag cttctcatcc ttcactattt taaaaggaca ttaccaataa
     3121 atggggagtc agacctaagg gcgaagaggc aaccatgccc aaaaagtaga tccgacacca
     3181 agttttgaag tgaagcccac ttctccccct gccgcttcta cgatagctct ggagatcgtc
     3241 aagccaagtc cggtaccctg cgagaactca ttcaatttct caaaacgggt aaatactttg
     3301 tcatgcaaat catccggtat accgattccc gtatcctcaa catagatacg aagaccttcc
     3361 ctttcaatgc catatcccat tttaatatat ccggatttgg tgcattttac cgcattagtg
     3421 aggaaattca tccatacttg cttaagccgg tttctatcca gaagtaccca gcactccgag
     3481 ttgggaccat ctaaacgtaa ctcgacattc ggatttgtca tcttcgcctg aatcgtaaca
     3541 aataactcat tacaaacctt ggccagattg aaattctcct tcctacgttc aattatgcct
     3601 gactctatct tggaaagatc aaggatatcg ttgatcaatc gcaataacaa ctcattattg
     3661 gattggatga tat
//
