LOCUS       c00323_gnlPro..         3653 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OOGJOHKA_323.
ACCESSION   c00323_gnlPro..
VERSION     c00323_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 13:54:59
            Original ID  :: gnl|Prokka|OOGJOHKA_323
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3653
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3653
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[28:3493]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      29..3493
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..3653
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3653
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             29..721
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg323_1"
                     /sec_met_domain="SucD_FumR (E-value: 3.1e-75, bitscore:
                     239.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWLLNSSIGKKLIMSISGLFLVLFLLFHLSMNVAAVFSGEAYNMV
                     CSLLGSNWYAVAATLVLAAGVVIHFVYAIILTLQNRKARGNDRYAINARPKGVEWASQN
                     MFVLGLIVILFMLLHFSQFWYKMMFAELIGHHEVALGSAMVSPQDGAAFINYYFQGNAV
                     ITVLYLIWYVALWFHLTHGFWSAIQTIGWNNTIWMNRWECISKIVATVICGLFAIITII
                     FFLNGVGA"
     CDS             770..2713
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 197.2; E-value:
                     1.7e-60)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg323_2"
                     /sec_met_domain="FAD_binding_2 (E-value: 8.3e-62, bitscore:
                     197.2, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 2.2e-39,
                     bitscore: 122.2, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSEINSKIPQGPLAEKWTNYKNHQKLVNPANKRRLDVIVVGTGLA
                     GASAAASLAEMGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKG
                     GDYRAREANVYRLAEVSNSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQT
                     GQQLLLGAYSALSRQIGLGKVKMYTRHEMLDVVKVDGRARGIIARNLITGKIERYAAHA
                     VVVATGGYVNTFFLSTNAMASNGSAAWQCYKKGAYFANPCMVQIHPTCVPVHGDYQSKL
                     TLMSESLRNDGRIWVPKKLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDV
                     ASRAAKERCDAGFGVNNTGLAVFLDFSDAINRLGKEVVKQKYGNLFDMYEEITNDDPYE
                     TPMMIYPALHYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYF
                     VLPYTIQNYLSDQIQVPRFSTDLPEFNEAEKNIQARINKLMSIKGKESVDSIHKRLGHI
                     MWEHIGMARNAESLKEAIQMLKDLKKEFWSNVFIPGNADDLNVELEKALRLADFIEIGT
                     LMAHDALDRAESCGGHFREEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNY
                     EFVVPQTRNYKK"
     CDS             2738..3493
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg323_3"
                     /sec_met_domain="Fer2_3 (E-value: 1.9e-26, bitscore: 80.0,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 6.7e-13, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 7.1e-07, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDINITLKVWRQKGPNVKGQFETYQLKNINQGTSFLEMLDILN
                     EQLISEGKEPVVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFNDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFTSVNTGGVPDANAIPIPKPDAEMAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
ORIGIN
        1 acaattaaat aacttaaatt attatcttat gtggttactt aattcttcta tcgggaagaa
       61 gctgataatg agcatatcag gtctcttcct tgttttgttc ttattgttcc acctgtcgat
      121 gaacgtggct gccgttttct ccggagaggc ttataacatg gtttgttcgc tgttgggctc
      181 taattggtac gctgtggcag ctactctcgt attggccgca ggcgttgtga tccacttcgt
      241 gtacgctatc attcttacct tgcaaaaccg taaggcacgt ggtaacgacc gttacgctat
      301 caacgctcgt ccgaaaggcg tagaatgggc atctcagaac atgttcgttt tgggtttgat
      361 cgttatcctg ttcatgctgt tgcacttcag ccagttctgg tataagatga tgttcgccga
      421 attgatcggt catcacgaag tagcattggg gtcagctatg gtatctccac aagacggagc
      481 cgcttttatc aactattact tccaaggtaa tgccgtgatc accgttcttt atttgatctg
      541 gtacgtggct ttgtggttcc acttgactca cggtttctgg agcgctatcc aaacaatcgg
      601 ttggaataac acgatctgga tgaaccgttg ggaatgtatc tctaagatcg tagctacggt
      661 tatttgcgga cttttcgcaa tcattactat catcttcttc ctaaacggag taggtgctta
      721 attgcaagct acaggtttta taattaagaa cttttgaaat taaatcatta tgtctgaaat
      781 aaattctaaa atacctcaag gcccgttggc tgagaagtgg actaattata aaaatcacca
      841 gaaattggtg aaccccgcta acaagcgtcg tttggatgtt atcgtagtcg gtacaggttt
      901 ggctggagct tccgctgccg catctttggc agagatggga ttcaacgtat tgaacttctg
      961 tatccaagat tctccccgcc gtgcgcactc tatcgccgct caaggtggta tcaacgccgc
     1021 taagaactac cagaatgatg gtgactctgt ttaccgtttg ttctacgata cgatcaaagg
     1081 tggtgactac cgtgctcgtg aggctaacgt ttatcgtttg gctgaggttt ctaactcaat
     1141 catcgaccag tgcgtggctc aaggcgttcc tttcgctcgc gaatatggtg gtttgttgga
     1201 taaccgttca ttcggtggcg ctcaggtatc gcgtacgttc tacgcccgtg gccagacggg
     1261 tcagcagctt ttgttaggcg cttattcagc gttgagtcgt cagatcggtt tgggtaaggt
     1321 taagatgtac actcgtcatg agatgttgga tgtagttaag gtagacggtc gtgctcgtgg
     1381 tatcatcgcc cgcaacttga ttacaggtaa gatcgaacgt tacgccgctc acgctgtagt
     1441 tgtagctaca ggaggttacg ttaatacgtt cttcttgtca accaacgcga tggcatctaa
     1501 cggttctgcc gcatggcaat gctacaagaa aggtgcttac ttcgctaatc cttgtatggt
     1561 tcagattcac ccgacttgcg taccggtaca cggtgactat cagtctaaat tgactttgat
     1621 gtccgagtct ttgcgtaatg atggccgtat ctgggttccg aagaaactgg aagacgctaa
     1681 ggctttacag gcaggaacaa agaaaggtaa agatatacct gaggcagatc gcgattatta
     1741 tttggaacgt cgttatccgg cattcggaaa cttggttcct cgcgacgtgg cttctcgtgc
     1801 cgctaaagag cgttgcgacg ctggcttcgg tgttaataac acaggtttgg ctgtattctt
     1861 ggacttctcc gacgctatca accgtctggg taaagaggtt gtaaaacaga aatatggtaa
     1921 cttgttcgat atgtacgaag aaatcaccaa cgacgatcca tacgagactc cgatgatgat
     1981 ctacccggca ttgcactatt caatgggtgg tctgtgggtt gattatgaat tgatgacttc
     2041 tatcccgggt ctgttcgcta tcggtgaggc aaacttctct gatcacggcg caaaccgttt
     2101 gggtgcttcc gcgttgatgc aaggtttggc cgatggttac ttcgtattgc cttatacgat
     2161 ccagaactat ttgtctgacc agattcaggt tccgcgtttc agcaccgatc tgcctgagtt
     2221 caacgaggct gagaagaata tccaagcccg tatcaataaa ttgatgagca tcaagggtaa
     2281 agagtctgtt gattctatcc acaaacgttt gggacacatc atgtgggaac acatcggtat
     2341 ggctcgtaac gcagagagct tgaaagaggc tatccagatg ttgaaagact tgaagaagga
     2401 attctggagc aacgtattca ttccgggtaa cgcagatgac ttgaacgtag agttggagaa
     2461 agcgttacgt ttggcagact ttatcgagat cggtaccttg atggcacatg acgctttaga
     2521 ccgtgctgaa tcttgtggtg gtcacttccg tgaggagcac cagactgagg aaggcgaggc
     2581 attacgtcac gatgacaaat tcatgtatgt ctcttgctgg gaatatcagg gtgaggataa
     2641 agaccctgtt atgctgaagg aagatttgaa ctatgaattc gtagttcctc agacacgtaa
     2701 ctataagaag taatcaatca ttgcaaaaat tagaatcatg gataaagata taaatatcac
     2761 actcaaagtt tggcgtcaga aaggtccgaa tgtaaaagga caattcgaaa cttaccaact
     2821 taaaaacatc aaccaaggca cttctttctt ggaaatgttg gatatcctga acgagcaatt
     2881 aatcagcgaa ggtaaagaac ctgttgtttt tgaccatgac tgtcgtgaag gtatctgcgg
     2941 tatgtgttca ttgtatgtaa acggacatcc gcacggaccg gcaaccggag ctacgacttg
     3001 ccaattatat atgcgccgtt tcaacgatgg tgagactatc acgatcgaac cgtggcgttc
     3061 cgccggtttc ccggtaatcc gcgacttgat ggtagaccgt tacgcttacg ataaaatcat
     3121 tcaagctggt ggtttcacat ccgtaaatac cggtggtgta cccgacgcaa acgcaattcc
     3181 tattcctaag ccagacgcag agatggcaat ggatgccgct gcttgtatcg gttgtggcgc
     3241 ttgtgccgcc gcttgtaaga acggttcagc tatgttgttc gtatccgcta aggttagcca
     3301 gttggcttta ttacctcaag gtaaggtaga ggctgcccgc cgtgcgaaag caatggtcgc
     3361 taaaatggat gaacttggct tcggtaactg tacgaacaca cgcgcttgcg agatggagtg
     3421 tccgaagaac atctccgtta gtaacatcgc tcgtttgaac cgtgagttca tttgcgctaa
     3481 gctacaagac taatatttga ttagtatatc atggaaaagg cgtctttctc taaaagaaag
     3541 gcgccttttt atttttgtta tatgttgaaa aacataattt agcactacag atcttgacag
     3601 gaattgaaaa gtccgtatat ttgcattgtg gttttttcat aatagtatta gat
//
