LOCUS       c00070_gnlPro..         4540 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|JHMKDKEP_70.
ACCESSION   c00070_gnlPro..
VERSION     c00070_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 13:14:38
            Original ID  :: gnl|Prokka|JHMKDKEP_70
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4540
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4540
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[313:3803]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      314..3803
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..4540
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4540
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             complement(314..1081)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052:Succinate dehydrogenase-2Fe-2S iron-sulfur
                     cluster binding domain (Score: -1.5; E-value: 8.6)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg70_1"
                     /sec_met_domain="Fer2_3 (E-value: 8e-25, bitscore: 75.5,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 6e-12, bitscore: 34.5,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 3.4e-07, bitscore: 19.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTLNLTLKVWRQENREAKGRIETYAAKDIPVDASFLEMLDIVN
                     ESLVKEGKEPIHFDHDCREGICGMCSLTINGIPHGPERQVTTCQLHMRKFKDGDTVWIE
                     PFRAKAFPILRDLMVDRSALDRIIAAGGYIDVRTGSAPNANNIAVEKVKADAAFDAAAC
                     IGCGACVASCKNASAMLFTSAKIAHLNLLPQGQPERSKRVLAMMKQMEAEGFGNCTNQY
                     ECEAACPKGISVDNIARLNRDFMRANAKEGLCF"
     CDS             complement(1122..3080)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 176.1; E-value:
                     4.2e-54)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg70_2"
                     /sec_met_domain="FAD_binding_2 (E-value: 3.3e-55, bitscore:
                     176.1, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 9.5e-39,
                     bitscore: 120.8, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINFPVSDWMPDAKIPSGPIQDKWSKYKLESKLINPNNKRKYTVL
                     VVGSGLAGASAAATLAELGYNVKCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRL
                     FYDTVKGGDFRAREANVYRLAEVSSNIIDQCVAQGVPFGREYGGLLDNRSFGGAQLKRT
                     FYARGQTGQQLLLGAYQALEKEVAKGHIEMFSRHEMMDLVLVDGEAKGIVTRNLVTGEI
                     KSYAGDTVLLCTGGYGNVFFLSTNAMGCNVTAAYKAYKRGAFFANPCFTQIHPTCIPVK
                     GDYQSKLTLMSESLRNSGRIWVPKSRETAEKIRKKEIKPSDVPEEERDYYLERKYPSFG
                     NLAPRDISSRAAKEACDDGRGVAVTGKGVFLDFADEIKRMGREWIDGQYGNLFDMYEEI
                     TDDDPHETPMMIYPASHYTMGGLWVDYNLMSNVKGLHVLGEANFSDHGANRLGASALMQ
                     GLSDGYFVIPATLPAYLTTTKPGAVTTAAPEFKAAEDEVKERINKLLNVKGTKTVDSFH
                     RELGLIMWEDCGMARTEESLTHAIERIPQLRKEFWENVRVTGTADGINAELEKACRVAD
                     FLEFAQLLCYDALNRQESCGAHFRVEYQLPDGEAKRNDDEYAYVAAWEYTGVDSLPVLH
                     KEPLTFENVHLAIRSYK"
     CDS             complement(3102..3803)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg70_3"
                     /sec_met_domain="SucD_FumR (E-value: 2.1e-14, bitscore:
                     42.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MNALVKTICTFVTSSIGRKIIVALTGLCLVLFLAGHLAGNLLIFG
                     GPEWINTYAHGLHSMPEAALWGIRAGLAVIFIIHVWLTIQLKLENHAAREPYVFKNTIK
                     ATLSSRYMIYTGLTVLVFLIYHLYQYTLRVGYDPAQFTTFISDGTVETFDVYKMIVTGF
                     SNVWCSAFYILAVLMLFSHLRHGVQSIFQTVGVDSRKIRPFYNFIAIAYGAIICLGFIS
                     VPVSVLLGIIK"
     CDS             4004..4276
                     /locus_tag="ctg70_4"
                     /transl_table=11
                     /translation="MKRSKKEVLDADFAAARAGVLDVAAFLDRVDRGEGEADFRYHALM
                     DVLPLLSASGPGRVRAILEALSYRDDTVPETAAAKAACGAPNLSK"
     CDS             4309..4539
                     /locus_tag="ctg70_5"
                     /transl_table=11
                     /translation="MVCRTTDEYRAMAQAGCLAVGEPAFWAGYDRSSADGFRDYFVQLT
                     ECEPARAAKFGMDHYTWLCINPKEAQDVAFAR"
ORIGIN
        1 agcccttgac aggaggccat cccttccggg catcagtcta attcctgtaa tcaatgtttc
       61 tttctctcga agaaagatgg ggccgtcgct gtcggacggg ggcttgctta ggtttcgtcc
      121 ggcatttcca agtacggttt cctggattac tgggtgcctt ccagtgtaac atttattttt
      181 tacgctgcgc gacgatgagg aagcaactgt ctgatatttt attcctggtt caaggaaacc
      241 tgataatgaa aaaaactccc gacagccgat gccgccggga gtttttacag agcgtcttaa
      301 gaaggaatct tctttagaaa cagagacctt ccttggcgtt ggcccgcatg aagtcgcggt
      361 tgaggcgggc gatgttgtcc acgctgatgc ctttcgggca ggcagcttca cattcatact
      421 ggttcgtgca gttgccgaag ccttccgctt ccatttgctt catcatggcc aggacgcgct
      481 tggaacgttc cggctggccc tgcggcagca gattgagatg ggcgatcttg gcggaggtga
      541 acagcatggc ggaggcgttt ttacaggagg ccacgcaggc gccgcagccg atgcaggcgg
      601 ccgcgtcaaa ggcggcgtcc gccttcactt tttccacggc gatgttgttg gcgttcggcg
      661 cggaaccggt acgcacgtca atatagcctc cggcggcgat gatgcggtcc agggcggagc
      721 ggtccaccat caggtcccgc aggattggga aagccttcgc gcggaagggt tcaatccaga
      781 cggtatcgcc gtccttgaac ttgcgcatgt gaagctggca agtggtcacc tggcgttccg
      841 gcccgtgggg aatgccgttg atggtcaggg agcacatgcc gcagatgcct tcccggcagt
      901 cgtggtcaaa atggatgggt tccttgcctt ccttgaccag gctttcattg acgatgtcca
      961 gcatttccag gaaggaggcg tccaccggaa tgtccttggc ggcataggtc tcaatgcggc
     1021 ccttggcttc ccggttttcc tggcgccaga ccttgagagt gagattgagt gttttagcca
     1081 tgttataatc tttcttttga gtgttaatgt attggctatc tttacttgta gctgcggatg
     1141 gcaaggtgca cgttctcgaa ggtcagcggt tccttgtgga gaacaggcag gctgtccacg
     1201 ccagtgtatt cccaggcggc tacataggcg tattcatcgt cgttgcgctt ggcttcgccg
     1261 tccggcagct ggtattccac gcggaagtgg gcgccgcagg attcctggcg gttcagggcg
     1321 tcatagcaga gcagctgggc gaattccagg aagtcggcca cgcggcaggc tttttccagt
     1381 tccgcgttga tgccgtccgc cgtgccggtt acgcgcacgt tttcccagaa ttccttgcgg
     1441 agctggggaa tgcgttcaat ggcgtgggtc aggctttctt ccgtgcgggc catgccgcag
     1501 tcttcccaca tgatgaggcc cagttcccgg tggaaggagt ccaccgtctt ggtgcccttc
     1561 acattcagga gcttgttgat gcgttccttc acttcgtctt ccgcagcctt gaattccggg
     1621 gcggcggtgg tgacggcacc gggcttggtg gtcgtcaggt aagcgggcaa cgtggcggga
     1681 atgacgaagt agccgtcaga caggccctgc atcagggcgg aggctcccag gcggttggcg
     1741 ccgtggtcgg agaagttggc ttcaccgagg acgtgcaacc ccttcacgtt ggacatgagg
     1801 ttgtagtcca cccacaggcc gcccatggtg tagtgggagg cggggtagat catcatgggc
     1861 gtttcatggg ggtcgtcgtc cgtgatttct tcgtacatgt caaacagatt accgtattgg
     1921 ccgtcaatcc attcgcggcc catgcgcttg atttcatccg caaaatccag gaagacgccc
     1981 ttgccggtga cggccacgcc gcggccgtcg tcgcaggctt ccttggcggc gcgggaggaa
     2041 atgtcgcgcg gagccaggtt gccgaaggag ggatacttgc gttccaggta gtagtcccgt
     2101 tcttcttcgg gaacatcgga aggcttgatt tcctttttac ggattttttc tgcggtttca
     2161 cgggacttgg gcacccagat gcggccgctg ttgcggaggg attccgacat cagcgtcagt
     2221 ttggactggt agtcgccttt gaccggaatg caggtggggt ggatttgcgt gaagcagggg
     2281 ttggcgaaga aggcaccacg cttgtaggct ttgtaggcgg cagtgacgtt gcaacccatc
     2341 gcattggtgg acaggaagaa cacgttgccg tagccgcccg tgcagagcag gacggtatcc
     2401 cctgcgtagg atttgatttc gccggtaacc agattgcggg tgacgatgcc cttggcttcg
     2461 ccgtccacca acaccagatc catcatttca tggcgggaga acatttcaat gtgtcccttg
     2521 gccacttcct tttccagggc ctggtaggca cccagcagga gctgctggcc cgtctgtccg
     2581 cgtgcgtaga aggttcgctt gagctgggcg ccgccgaagg agcggttgtc cagcaggccg
     2641 ccgtattcac ggccgaaggg aacgccctgg gccacacact ggtcaatgat gttggaggag
     2701 acttccgcca gacggtagac attggcttca cgggcgcgga agtcgccgcc tttaacggtg
     2761 tcgtaaaaca ggcggtaaac ggagtcgccg tcgttctggt agttcttggc tgcgttaatg
     2821 ccgccctggg ccgcaatgga gtgggcgcgg cgggggctgt cctggtagca gaagcatttg
     2881 acgttgtagc caagttcggc aagcgtggcg gcagcggagg cgccagccag gccggaacct
     2941 accacaagca ccgtgtactt gcgcttgttg ttcggattga tcagtttgga ctcaagtttg
     3001 tacttggacc atttgtcctg aatagggccg gagggaattt tggcatccgg catccagtcg
     3061 cttacgggaa aattaatcat gactttgaga ggagttgatg gttatttgat gatacccaga
     3121 agaaccgaga ccggcacgga gatgaagccc aggcagataa tcgcgccgta ggcgatggcg
     3181 ataaagttgt agaagggccg gattttgcgg gaatcgacgc ccacagtctg gaagatggac
     3241 tgtaccccgt ggcgcagatg gctgaacagc atcagaacgg ccaggatgta gaaggcggag
     3301 caccatacgt tggagaatcc ggtcacgatc atcttgtaca cgtcaaaggt ttccaccgta
     3361 ccgtcggaaa taaaggtggt gaactgggcg gggtcgtatc ccacgcgcag ggtgtactgg
     3421 tacaggtggt agatcaggaa caccaggacg gtcaaaccgg tatagatcat gtagcgggag
     3481 gagagcgttg ccttaatggt gttcttgaac acgtagggtt cgcgggccgc atggttttcc
     3541 agcttcagct ggatggtcag ccagacgtgg atgatgaaga tcaccgcaag ccccgcgcgg
     3601 attccccaga gggctgcttc cggcatggag tgcaggccgt gggcgtaggt gttgatccat
     3661 tcagggcctc cgaagatgag aaggttgcct gccaggtgtc ccgcaaggaa caagaccaaa
     3721 cataaccccg tcagagccac aatgattttg cggccgatgg aggatgtcac gaatgtacat
     3781 atggttttta ctagtgcatt cataggcgct ctaatgaaat aaatgcgtct tcacaggatg
     3841 cccggttcaa gcccggatgg caagcctctt cttgaatttc cggcgctttt cggatttttg
     3901 aaaaaccggg gaaagaggtt gctgtttatc agtttggaat ggttatcatt ttgttgcctg
     3961 ttccggcttg acgaggcttt ccgcataggc gtatcgtctg tgcatgaaaa gaagtaaaaa
     4021 ggaggtgctg gacgcggatt tcgcagcggc gcgcgccggc gttctggatg tggccgcatt
     4081 tctggaccgg gtggaccgcg gggaagggga ggcggatttc cgctaccatg ccctgatgga
     4141 tgttcttccc ctcttgtccg catccggccc cggacgcgtc cgggccattc tggaagccct
     4201 ttcctacagg gatgacaccg tgccggaaac cgccgcagcc aaagcggcct gcggagcccc
     4261 gaacctcagc aaataagaat gatggctttc atagaacctc atatacatat ggtgtgccgc
     4321 accacggacg aataccgggc catggcgcag gccggctgcc tggcagtagg ggaaccggcg
     4381 ttctgggccg gttacgaccg ttcgtccgcg gacggtttcc gggattattt cgtacagctt
     4441 acggaatgcg aaccagccag ggcggccaag ttcggcatgg accattacac atggctgtgc
     4501 atcaatccca aggaggcgca ggacgtggct ttcgcgcggg
//
