LOCUS       c00241_gnlPro..         3449 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|COIINBLA_241.
ACCESSION   c00241_gnlPro..
VERSION     c00241_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 13:27:17
            Original ID  :: gnl|Prokka|COIINBLA_241
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3449
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3449
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:1514]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..1514
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3449
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3449
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..718
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg241_1"
                     /sec_met_domain="Gly_radical (E-value: 1.6e-47, bitscore:
                     148.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 1.4e-40, bitscore:
                     126.9, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="PEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTE
                     GEFPCYGNNDPRVDDIACSLVSNFMEKLRRLHTYRNSLPTQSILTITSNVVYGKKTGNT
                     PDGRRAGEPFAPGANPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEE
                     RERRVKLVSLLDAYFAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFIKL
                     TREQQQEVINRTFHTR"
     CDS             735..1514
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 53.6;
                     E-value: 9.1e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg241_2"
                     /sec_met_domain="Radical_SAM (E-value: 2e-32, bitscore:
                     100.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHNPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRRGGFPDCVH"
     CDS             complement(1763..3448)
                     /locus_tag="ctg241_3"
                     /transl_table=11
                     /translation="QKLSRENFKRTIANFLCSAKTATLTDEQLKHCVTVGGHEQFAAPV
                     LEGRGQVCAIAHSGNWEALARIRAFYPEVERYGSMYRQFDNPLMEEYVYQRRTERGTQM
                     FSKEGGIKAPMKMLKEGGALGVLSDQFVWEGVYVPFFGKVTGTTPLPALLRKRAGADMV
                     AIAVRTDAPGHWIADMGNVVDFSRSDGSLAGDTIEVNRGLETLIRESVLDVFWMHHRWK
                     SVDRFAPQDKKTAALLENMELKPYRILVAVPRALDEALATVPLIRALKTVRCDMQVNIV
                     CPSAQLGIWKTVPEVTHVLPHDSLKQLREALVADEFYNDGPLDLGVMLDGDMETLKALE
                     PYGPMMFSGLDTHPGARKYKFRVKAPVLRAAPPAHRVQLYLQLGVLHGLDVATPSLFPV
                     KKAAPAEDAPILIAPFSRLGSASEWSREQWRELVSLLPGRTVLAALEEDRERAASLAEH
                     LNVELAVGAPEALFPVMDGAAAAVAVDGDFPSLCSFRGLPVVTLFSTRLPDVYRPMGTF
                     NRSLYSHQCCSPCFRKECDRDVPCNRHIAVRDVLEALREISEKE"
ORIGIN
        1 tccggaaatt ctgcgcacga tggctacggg aatcgccggg ctttccgtgg cggcggactc
       61 cctgtccgcc atcaagtatg ccaccgtaaa agccatccgc aatgaggaag ggctgattgt
      121 ggacttcaaa acggaagggg aattcccctg ttacgggaac aatgacccgc gcgtggacga
      181 catagcatgc agcctggtca gcaatttcat ggaaaaactg cgcaggctcc acacttaccg
      241 caattccctg cccacccagt ccatcctgac gatcacctcc aacgtggtgt acggcaaaaa
      301 gacgggcaac acgccggacg gccgccgagc cggggaaccg ttcgcccccg gagccaaccc
      361 catgcacgga agggacagga acggagccgt ggcctccatg ctctccgtag ccaagctgtc
      421 ctatgacgac tccctggacg gcatctccta caccttctcc atcgttcctc aggccctggg
      481 caaggaggaa cgtgaacgcc gcgtcaagct cgtctccctg ctggacgcct actttgccgc
      541 tacaggtcac cacattaatg tgaacgtact ggaacgggaa acgcttctcg acgccatgga
      601 tcacccggaa aaatacccgc agcttaccat ccgcgtttcc ggctatgccg tgaatttcat
      661 caagctgacc cgggaacagc agcaggaggt catcaaccgc actttccaca cccgttaacc
      721 cggactgcaa gcggatgccg caggtttttc cacaaaacca taatccggac ggagaaagct
      781 ccgtcaccgg tttggttcat tccgtggaat cctgcggcac cgtggacggc cccggcatcc
      841 gcttcgtcct tttcctgtcc ggctgcagcc tgcgctgccg ctactgccat aatccggata
      901 cctcctacgt gcggaggggc cggctcagga gcgcagatga tgtcctcaag gaaatagccc
      961 gttaccggga ttttttgcag gcggcgggcg gcggagtcac cctctccggc ggggatcccc
     1021 tcttccagcc ggattttgcc ggtgccgtgc tgaaaggctg ccggaaactg ggcctgcaca
     1081 cctgcctgga cacctccggc catctgggcg tgaatgcggg cgaagaaatg ctggcggata
     1141 cggacctggt cctgctggat atcaaggcat ggaatccgaa acggtatcgc gccctgacag
     1201 gcggggaact gcgccccaca ctggagtttg cggaacgcct ggcctccctc cgcaagcccg
     1261 tgtggcttag gtatgtgctg gtacctggcg ttacggacaa tctggaagaa attgaggaac
     1321 tctcccgcca tgcggcgctt ctgggcaacg tggaacgcgt ggacgtgctc cctttccacc
     1381 agctggggag attcaaatgg gatgaattga agctggacta taccttgaag gacgtcccgg
     1441 aagcgtccgc agaactggca gaacaggtcc gcggcatttt ccggcgggga ggctttccgg
     1501 attgcgtgca ttaaccggat tttcgcgctt tggcgccacc aaccgcagca aaccggacgc
     1561 aggcatcccc ctcttccgga tgaggggccc tctcccctcc actgcagaat ccatgcaaaa
     1621 aggaagacgc atttctggaa ttgctccgga atacggcatt tccgcaattc ctttcgttat
     1681 ttcatcattc tttacggcaa cggcagcatt cccgccgttt tttccgccaa cgccggggga
     1741 cggcattccg tcccttccca gattattctt tctcggaaat ttcccgcaag gcttccagta
     1801 cgtcccgcac ggcaatgtgg cggttgcagg gaacatcccg gtcacattcc ttccggaagc
     1861 agggagaaca gcactggtgg ctgtaaaggg aacggttgaa tgtgcccata gggcggtaaa
     1921 catccggaag ccgggtggaa aacagagtca ccaccggaag cccccggaaa gaacagaggg
     1981 acggaaagtc gccatccacg gctactgccg ccgcagcgcc gtccatgacg ggaaagagag
     2041 cctccggcgc acccaccgcc agctccacat tcaaatgttc cgccagggag gcggcacgct
     2101 cccggtcttc ttccagagcc gccagcaccg tccgtcccgg cagaagggaa accagctccc
     2161 tccactgctc cctgctccat tcactggcgc tccccaggcg ggaaaacgga gcgattaaaa
     2221 tgggggcgtc ttccgccggg gccgcttttt tcacagggaa caaagacggg gtggcgacgt
     2281 ccagcccatg cagcaccccc aattgcagat aaagctgcac ccggtgcgcc gggggggcgg
     2341 cgcgcaggac gggggccttt acccggaact tgtacttccg ggcaccggga tgcgtgtcca
     2401 gcccggaaaa catcatgggg ccatacggtt ccagcgcttt cagggtttcc atatctccat
     2461 ccagcatgac gcccagatcc agcggtccgt cattgtaaaa ttcatctgct accaaagctt
     2521 cccgaagctg cttcagggag tcgtgcggca gcacatgggt aacttccggc accgtcttcc
     2581 atatccccag ctgggcagac gggcagacga tgttcacctg catgtcgcac cggacggttt
     2641 tcaaagctcg aatcagggga acagtcgcca gggcttcatc cagcgccctg ggaacggcta
     2701 ccagaatgcg gtacggcttc agctccatat tttccaggag cgcagccgtt tttttatcct
     2761 gcggggcaaa tcggtcaacg gatttccagc gatggtgcat ccagaacaca tccagcacgg
     2821 attcccggat caatgtttcc agccccctgt tcacttcaat cgtgtccccc gccagagagc
     2881 cgtccgacct ggaaaaatcc accacattcc ccatatccgc tatccagtgg ccgggagcat
     2941 ccgtgcgcac cgctatggcc accatgtccg cgccagccct ttttctcagc agagcgggca
     3001 gcggagtcgt gccggtcacc ttgccgaaaa aggggacata caccccctcc catacgaact
     3061 ggtcgctcag cacgcccagg gctccccctt ccttgagcat tttcatggga gccttgatgc
     3121 ctccctcctt ggagaacatc tgagttccac gttccgtgcg ccgctggtac acgtattcct
     3181 ccatcagcgg attgtcaaac tggcggtaca tggacccgta acgctccacc tccgggtaaa
     3241 atgcgcgtat tctcgccagc gcctcccagt tgccggagtg ggctatggcg caaacctgcc
     3301 cgcgcccttc cagcacaggc gcggcaaact gttcgtgccc tccgaccgtc acgcaatgct
     3361 tcaactgctc atccgtcagg gtggccgtct tcgcggaaca caaaaaattg gcaatggtcc
     3421 ttttgaaatt ttcccggctt aatttctgc
//
