LOCUS       c00248_gnlPro..         3304 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|COIINBLA_248.
ACCESSION   c00248_gnlPro..
VERSION     c00248_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 13:27:17
            Original ID  :: gnl|Prokka|COIINBLA_248
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3304
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3304
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:3105]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..3105
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..3304
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3304
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             3..317
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg248_1"
                     /sec_met_domain="SucD_FumR (E-value: 3.9e-09, bitscore:
                     24.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="YTLRVGYDPAQFTTFISDGTVETFDVYKMIVTGFSNVWCSAFYIL
                     AVLMLFSHLRHGVQSIFQTVGVDSRKIRPFYNFIAIAYGAIICLGFISVPVSVLLGIIK
                     "
     CDS             339..2297
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 176.1; E-value:
                     4.2e-54)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg248_2"
                     /sec_met_domain="FAD_binding_2 (E-value: 2e-55, bitscore:
                     176.1, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 5.7e-39,
                     bitscore: 120.8, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINFPVSDWMPDAKIPSGPIQDKWSKYKLESKLINPNNKRKYTVL
                     VVGSGLAGASAAATLAELGYNVKCFCYQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRL
                     FYDTVKGGDFRAREANVYRLAEVSSNIIDQCVAQGVPFGREYGGLLDNRSFGGAQLKRT
                     FYARGQTGQQLLLGAYQALEKEVAKGHIEMFSRHEMMDLVLVDGEAKGIVTRNLVTGEI
                     KSYAGDTVLLCTGGYGNVFFLSTNAMGCNVTAAYKAYKRGAFFANPCFTQIHPTCIPVK
                     GDYQSKLTLMSESLRNSGRIWVPKSRETAEKIRKKEIKPSDVPEEERDYYLERKYPSFG
                     NLAPRDISSRAAKEACDDGRGVAVTGKGVFLDFADEIKRMGREWIDGQYGNLFDMYEEI
                     TDDDPHETPMMIYPASHYTMGGLWVDYNLMSNVKGLHVLGEANFSDHGANRLGASALMQ
                     GLSDGYFVIPATLPAYLTTTKPGAVTTAAPEFKAAEDEVKERINKLLNVKGTKTVDSFH
                     RELGLIMWEDCGMARTEESLTHAIERIPQLRKEFWENVRVTGTADGINAELEKACRVAD
                     FLEFAQLLCYDALNRQESCGAHFRVEYQLPDGEAKRNDDEYAYVAAWEYTGVDSLPVLH
                     KEPLTFENVHLAIRSYK"
     CDS             2338..3105
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052:Succinate dehydrogenase-2Fe-2S iron-sulfur
                     cluster binding domain (Score: -1.5; E-value: 8.6)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg248_3"
                     /sec_met_domain="Fer2_3 (E-value: 1.1e-24, bitscore: 74.4,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 3.6e-12, bitscore: 34.5,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 2e-07, bitscore: 19.2,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTLNLTLKVWRQENREAKGRIETYAAKDIPVDASFLEMLDIVN
                     EGLVKEGKEPIHFDHDCREGICGMCSLTINGIPHGPERQVTTCQLHMRKFKDGDTVWIE
                     PFRAKAFPILRDLMVDRSALDRIIAAGGYIDVRTGSAPNANNIAVEKVKADAAFDAAAC
                     IGCGACVASCKNASAMLFTSAKIAHLNLLPQGQPERSKRVLAMMKQMEAEGFGNCTNQY
                     ECEAACPKGISVDNIARLNRDFMRANAKEGLCF"
ORIGIN
        1 agtacaccct gcgcgtggga tacgaccccg cccagttcac caccttcatt tccgacggta
       61 cggtggaaac ctttgacgtg tacaagatga tcgtgaccgg attctccaac gtatggtgct
      121 ccgccttcta catcctggcc gttctgatgc tgttcagcca tctgcgccac ggggtacagt
      181 ccatcttcca gactgtgggc gtcgattccc gcaaaatccg gcccttctac aactttatcg
      241 ccatcgccta cggcgcgatt atctgcctgg gcttcatctc cgtgccggtc tcggttcttc
      301 tgggtatcat caaataacca tcaactcctc tcaaagtcat gattaatttt cccgtaagcg
      361 actggatgcc ggatgccaaa attccctccg gccctattca ggacaaatgg tccaagtaca
      421 aacttgagtc caaactgatc aatccgaaca acaagcgcaa gtacacggtg cttgtggtag
      481 gttccggcct ggctggcgcc tccgctgccg ccacgcttgc cgaacttggc tacaacgtca
      541 aatgcttctg ctaccaggac agcccccgcc gcgcccactc cattgcggcc cagggcggca
      601 ttaacgcggc caagaactac cagaatgacg gcgactccgt ttaccgcctg ttttacgaca
      661 ccgttaaagg cggcgacttc cgcgcccgtg aagccaatgt ctaccgtctg gcggaagtct
      721 cctccaacat cattgaccag tgtgtggccc agggcgttcc cttcggccgt gaatacggcg
      781 gcctgctgga caaccgctcc ttcggcggcg cccagctcaa gcgaaccttc tacgcacgcg
      841 gacagacggg ccagcagctc ctgctgggtg cctaccaggc cctggaaaag gaagtggcca
      901 agggacacat tgaaatgttc tcccgccatg aaatgatgga tctggtgctg gtggacggcg
      961 aagccaaggg catcgtcacc cgcaatctgg ttaccggcga aatcaaatcc tacgcagggg
     1021 ataccgtcct gctctgcacg ggcggctacg gcaacgtgtt cttcctgtcc accaacgcga
     1081 tgggctgcaa cgtcactgcc gcctacaagg cctacaagcg cggtgccttc ttcgccaacc
     1141 cctgcttcac gcaaatccac cccacctgca ttccggtcaa gggcgactac cagtccaaac
     1201 tgacgctgat gtcggaatcc ctccgcaaca gcggccgcat ctgggtgccc aagtcccgtg
     1261 aaaccgcaga aaaaatccgt aagaaggaaa tcaagccttc cgatgttccc gaagaagaac
     1321 gggactacta cctggaacgc aagtatccct ccttcggcaa cctggctccg cgcgacattt
     1381 cctcccgcgc cgccaaggaa gcctgcgacg acggccgcgg cgtggccgtc accggcaagg
     1441 gcgtcttcct agattttgcg gatgaaatca agcgcatggg ccgcgaatgg attgacggcc
     1501 aatacggcaa tctgtttgac atgtacgaag aaatcacgga cgacgacccc catgaaacgc
     1561 ccatgatgat ctaccccgcc tcccactaca ccatgggtgg cctgtgggtg gactacaacc
     1621 tcatgtccaa tgtgaagggg ctgcacgtcc tcggtgaagc caacttctcc gaccacggcg
     1681 ccaaccgcct gggagcctcc gccctgatgc agggcctgtc tgacggctac ttcgtcattc
     1741 ccgccacgct gcccgcttac ctgacgacca ccaaacccgg cgccgtcacc accgccgccc
     1801 cggaattcaa ggctgcggaa gacgaagtga aggaacgcat caacaagctc ctgaatgtga
     1861 agggcaccaa gacggtggac tccttccacc gggaactggg cctcattatg tgggaagact
     1921 gcggcatggc ccgcacggaa gaaagcctga cccacgccat tgaacgcatt ccccagctcc
     1981 gcaaggaatt ctgggaaaac gtgcgcgtaa ccggcacggc ggacggcatc aacgcggaac
     2041 tggaaaaagc ctgccgcgtg gccgacttcc tggaattcgc ccagctgctc tgctatgacg
     2101 ccctgaaccg ccaggaatcc tgcggcgccc acttccgcgt ggaataccag ctgccggacg
     2161 gcgaagctaa gcgcaacgac gatgaatacg cctatgtagc cgcctgggaa tacactggcg
     2221 tggacagcct gcctgttctc cacaaggaac cgctgacctt cgagaacgtg caccttgcca
     2281 tccgcagcta caagtaaaga tagccaatac attaacactc aaaagaaaga ttataacatg
     2341 gctaaaacac tcaatctcac tctcaaggtc tggcgccagg aaaaccggga agccaagggc
     2401 cgcattgaga cctatgccgc caaggacatt ccggtggacg cctccttcct ggaaatgctg
     2461 gacatcgtca atgaaggcct ggtcaaggaa ggcaaggaac ccatccattt tgaccacgac
     2521 tgccgggaag gcatctgcgg catgtgctcc ctgaccatca acggcattcc ccacgggccg
     2581 gaacgccagg tgaccacttg ccagcttcac atgcgcaagt tcaaggatgg cgataccgtc
     2641 tggattgaac ccttccgcgc gaaggctttc ccaatcctgc gggacctgat ggtggaccgc
     2701 tccgccctgg accgcatcat cgccgctgga ggctatattg acgtgcgtac cggttccgcg
     2761 ccgaacgcca acaacatcgc cgtggaaaaa gtgaaggcgg acgccgcctt tgacgcggcc
     2821 gcctgcatcg gctgcggcgc ctgcgtggcc tcctgtaaaa acgcctccgc catgctgttc
     2881 acctccgcca agatcgccca tctcaatctg ctgccgcagg gccagccgga acgttccaag
     2941 cgcgtcctgg ccatgatgaa gcagatggaa gcggaaggct ttggcaactg cacgaaccag
     3001 tatgaatgtg aagccgcctg cccgaaaggc atcagcgtgg acaacatcgc ccgcctcaac
     3061 cgcgacttca tgcgggccaa cgccaaggaa ggcctctgtt tctaaagaag attccttctt
     3121 aagacgctct gtaaaaactc ccggcggcat cggctgtcgg gagttttttc attaccaggt
     3181 ttccttgaac caggaataaa ataccagaca gttgcttcct catcgtcgcg cagcgtaaaa
     3241 aattgttaca ccggaagggt tccagtaatc caggagaccg tacttggaaa tgccggacga
     3301 aacc
//
