LOCUS       c00008_gnlPro..        12825 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LECGHOHB_8.
ACCESSION   c00008_gnlPro..
VERSION     c00008_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 12:37:21
            Original ID  :: gnl|Prokka|LECGHOHB_8
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 12825
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..12825
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[860:7987]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      861..7987
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..12825
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..12825
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(41..742)
                     /locus_tag="ctg8_1"
                     /transl_table=11
                     /translation="MNWMIYPAARAFIKVCAVLFGAGAAAAVIIHLFMPFFLRSPRTAA
                     PFLLLGTGAELLSILSLSFLMFWGARVLLASRGSRITRNLSMLAFLPALYALLIPFAGM
                     PVPSQYTEMDRVWEAFLLLAAVTSFFSFPYTAGYEAAGRRLFLAWGIAAAGCGLQSALF
                     FLAILLRQFLSTAVPSLAAPVAWLAVLLSCGLEIMLATLSAILAQRLFKEVMSIASMPE
                     PIDPSMPGNRP"
     CDS             complement(861..2357)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: AcetylCoA_hydro"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: AcetylCoA_hyd_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg8_2"
                     /sec_met_domain="AcetylCoA_hyd_C (E-value: 1.2e-46,
                     bitscore: 148.6, seeds: 181, tool: rule-based-clusters)"
                     /sec_met_domain="AcetylCoA_hydro (E-value: 6.4e-44,
                     bitscore: 140.4, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYKVLTPEEAASLIKHGHHIGLSGFTPAGTAKAVTAALAKIAEA
                     EHEKGNPFQVSVFTGASTGDSCDGILSRAKAIRYRAPYTTNVDFRKAVNNGEIAYNDIH
                     LSQMAQEVRYGFMGNVDIAIIEACEVTADGKIYLTAAGGIAPTICRLADKIIVELNAAH
                     SKSAMGLHDVYEPLDPPYRREISIFKPSDRIGKPYIQVDPKKIVGVVETNWPDEARSFA
                     AADPLTDKIGQNVADFLAADMKRGIIPSTFLPLQSGVGNIANAVLGALGRDKTIPAFEM
                     YTEVIQNSVIGLIREGRVKFGSACSLTVTNDCLEGIYRDMDFFRDKLVLRPSEISNSPE
                     VIRRLGVISINTAIEADIYGNVNSTHIGGTKMMNGIGGSGDFTRNAYISIFTCPSVAKE
                     GKISAIVPMVSHHDHTEHDVNIIITEQGVADLRGKSPKERAQTIIENCVHPDYKNILWD
                     YLKLSDGKAQTPQSIRAALGMHAELARSGDMKNVDWAQYK"
     CDS             complement(2418..3197)
                     /locus_tag="ctg8_3"
                     /transl_table=11
                     /translation="MNSAASFSTPPEEEGPCGDWTYHEWAEAFSTNDLARALPPGHIAV
                     CRVQTGGRGRFNRKWIGEEGGLWASFTVPLSAPEDTAAGVHWGHLPLVAGLALFNMLRG
                     MGLDSVRLRWPNDLLAGKSKLAGILVERPAENMAVIGIGINVFNDVSSIAGEIKDPPAR
                     LADLVRDCPSLHAVMASLGSHLNQVLSVFSHGGVPALQEGLSRAWAGERPVSILTDDET
                     VRGIFTGIDDQGNPVLSLPAGTVRTIPAHLINRLLEE"
     CDS             complement(3235..4860)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg8_4"
                     /sec_met_domain="OAD_beta (E-value: 1.9e-159, bitscore:
                     520.2, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGVFSLSWQMVGMWGIAILLLYLGVAKQYEPLLMVPIAFGALIAN
                     IPDNGMLITQLNQQVISSNEQGEVTATSLNNVGYLRVHVAPLQQAPSKVPANLTTPESR
                     AQYQEIMQQPMQVYPGSQLTVSKIKSVRESQEKAKADAALLGDDSLTVDPNLKDFQNVT
                     EDNGNEPVFLLTNGEGTTVVRQQGVNYFDTSGNRIPVDLKTQKLEPLVVSAAGKYVAVG
                     QHTQELLVTSIHGGLYDWIGLGIKAEIFPPIIFLGVGALTDFGPLLAAPRTLLLGAAAQ
                     VGVAATFFMALFMGFTPNESASIGIIGGADGPTSIFLTMKLAPHLLGAVAVAAYTYMSL
                     VPLIQPPIMALLTTKKERLIRMKSLRTVSKSEKLFFAVLVTIVTILLIPDASPLIGMLM
                     LGNFLRECKVTERLVQASQNEIINIVTIFLGTSVGLTMQGDRFLQSETLLIILLGIVAF
                     GVATAGGVIAAKIMNLIWRKNPVNPLIGSAGVSAVPMAARVSHNVGQKYDPSNYLLMHA
                     MGPNVAGVIGTAVIAGYYIATLAK"
     CDS             complement(4978..5379)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 58.4; E-value:
                     1.6e-18)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg8_5"
                     /sec_met_domain="Biotin_lipoyl (E-value: 4.2e-19, bitscore:
                     58.4, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKLRITVDGKVFDVSVELLDQVSSTTAAPAPAPVAAPAPAASAP
                     VAAPAPAPAPAPAPAAAGAGDVPSPLAGKVVSLDVAAGTPVKAGDQILTLEAMKMNTII
                     YAPASGTLTAFCVNPGDTVQEGQALAKIG"
     CDS             complement(5452..5898)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg8_6"
                     /sec_met_domain="OAD_gamma (E-value: 3e-10, bitscore: 30.8,
                     seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLTTLAFAFAQGIANVMNNLNYQVVGIMVVMLCLGGMAVILTVS
                     GSIAAAIQNRAKTAAPVAVPAAAPAAAGKPGMTPEMLAILSAAVDSAMTELTPEMVAVI
                     SAAVDAGLDGMSHRIVEIKQSPGSGYAAAGRAEIFASHRIRPSR"
     CDS             complement(5920..7485)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg8_7"
                     /sec_met_domain="CT_Vparvula (E-value: 1.5e-216, bitscore:
                     709.8, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAIDPKLIDDLNSRRKKVILSGGQEKIDKRHEKGEMTARDRMGYL
                     FEEGTFSEIGMHVRHNCHNFGMGKKEIPGDGVVSGFGLVDGRPVACAASDFLAQGGSLG
                     YMHAMKIADAQKYALKAGIPMVTVNDSGGARLQEGVAALSGYAQVFYNNVLASGVVPQI
                     SMILGPCAGGAAYSPALTDFIIMRNSGNAGMYITGPKVIEQVTYEKCTMDDIGSAAIHA
                     TVSGNVHFVADSDAHAMDILKRLLSFLPSNNTEEPPHKLDTPLDLSADEGMSDLIPGDN
                     RTPLDVQPIISRLVDNGDFLEVHKDFAKNVVVGFGRICGVVVGIIANQPNVKAGCLDID
                     SSDKAARFIRFCNAFNIPLVNLVDVPGFLPGKNQERGGIIRHGAKLIFAYSQATVPKVT
                     LIMRKAYGGAYIAMCCKDLGADAVFAWPGAEIAVMGAEGAVPVLYGRELKAVEDPAEKA
                     KRQGELLEEYREAFYNPYVAAGMGQITEVINPEETRAKIAFALRTLLNKKEVRPAKKHG
                     NIPL"
     CDS             complement(7517..7987)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg8_8"
                     /sec_met_domain="Glyoxalase_4 (E-value: 1.2e-25, bitscore:
                     80.1, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYCRPPPCIPYNHHTHMNQQIDHIGIAVKSLDATVPYYRDALGLG
                     EPHIEEVPTQKVRVAMFDVAGVHIELLEPTSPESAIAKAIEKKGEGIHHIAFKTNDIAG
                     NISQAKEAGLTVLNDPPVPGAHNTQVTFLHPKCTFGVLTEFCQHPGSCECGK"
     CDS             8105..8779
                     /locus_tag="ctg8_9"
                     /transl_table=11
                     /translation="MFKSGLAVLFLLASWFSAACAYELLPAHVAVVYNGKSELSRRMAR
                     EYARVRGVPEGNLVSLDCPMTSEISRKEYEDTIRVPLLEAARKQRWWVPSGIASSPLMN
                     RKIFVLVLMADLPMKIRHETPAPLPGKGVNQMQTDRAAVDSELALLAVGGYERKSWQVN
                     PYFNKREDFVGSGLPSFLVCRLDGLTPDTCMRLVAEPANVEKKGLWGWARGTGGWTTCS
                     SA"
     CDS             8728..9615
                     /locus_tag="ctg8_10"
                     /transl_table=11
                     /translation="MGVGQGDRWLDDVFERVREAGIPAYLDDWPQTLPEKFPLSRDTAL
                     YCGWYAGNANGPFSDPSFRFRPGAIAMHLHSFSAADFKVPGRGWSSALLEKGAAVTVGN
                     VYEPFLGACHRFDIFVDRLLDGYTVAEASWMSMPVLSWQGVVFGDPLYRPYARMKDMDV
                     EPTEEDRYFQGWWASSVQFGDRWKDRSARLMESARKAPFSCLYEALALECLYRKEPARA
                     GELLSSALDGAADARTRARLLLEILMAERARGGNKAFLQRADGIRGLMSSSAFLPALEE
                     WLARVAPPPAPPKK"
     CDS             9725..9898
                     /locus_tag="ctg8_11"
                     /transl_table=11
                     /translation="MNIINKIEQEQLKADVTPFNVGDTIKVHTRVIEGGKERIQIFQGI
                     VIAXXXXRFRHQ"
     CDS             9936..10079
                     /locus_tag="ctg8_12"
                     /transl_table=11
                     /translation="VERVFPLHTPRIAKIEVVNRGKVRRAKLHYLRGRIGKDAMIVKSA
                     NR"
     CDS             complement(10195..10401)
                     /locus_tag="ctg8_13"
                     /transl_table=11
                     /translation="MECHALSSGIGFAYEIFRFLLHSPGAIQARTEENPKNVFAPDRKQ
                     PPFSTFPTPEKRSHPHMAPGEIS"
     CDS             complement(10504..11934)
                     /locus_tag="ctg8_14"
                     /transl_table=11
                     /translation="VEQSHQGLLRVYEVGQAGPFTFIISEHSPYPRLADMETLPQISPQ
                     AALNLVRNMAEGLLTLHRKNIFHGGITPKLICLREDGGDALLLPINIYPAQPPVDMGDF
                     ASPEWVTGAETTFTPGMDIYALGLALYILLTRKTPMEAGFAMPSSLIKCSDAVDSAVSR
                     AINPDTRERYRDLGDFITDLDKAIANPAGRNAASIPPPPSSPAIPALNVQKGQSNIYYY
                     LIPALIVGIVLTYTCILYKKDVVRMRNDYNEQVQKENNAKAEAARQANRDARRHAPATA
                     QNAAAAIPSPVPAARPAQAPAVPKVSAEPGKVNWSLQPGVKVRQSSNRNMLGAYGPEKA
                     VDGNTSSRISDVSISSTGVVEGKTAWFGIDFGRETNRTIEKVVIYTPANLTLLGTMEKF
                     KVILYGNDKNVLAEKTFSTTPSEKSTNVTSWKLDAPVKARALRVESANPSQPLALTEVE
                     AYGPEDAEDTPAPAPAEK"
     CDS             complement(11892..12137)
                     /locus_tag="ctg8_15"
                     /transl_table=11
                     /translation="MSADDSFPMDQISSLFPQLENIQLVGAKGPQQIFTATLKSNFAPV
                     MLRVVPTEEAGIXXXXSSAPSPSWNNPTRGSCAFMK"
     CDS             complement(12607..12825)
                     /locus_tag="ctg8_16"
                     /transl_table=11
                     /translation="HSSHLDWSASEKIVPSGHSVQDDPASAVELRRILREHLVKVKGRK
                     TLEEADAKAATPVWQANPSPPIILKRP"
ORIGIN
        1 tgctcttccg gcaggccggt aaaaacggag gggaggaaag tcagggacgg ttgcccggca
       61 tggaaggatc aatcggttcc ggcatggatg ctatggacat gacctcttta aacaggcgct
      121 gcgccagaat agcggaaagg gtcgccagca tgatttccag gccgcaggac agaagaacgg
      181 ccagccatgc aacaggcgct gccagggagg gaactgcggt ggacaggaac tgacgcaaca
      241 ggatggcaag aaaaaacagg gcgctctgca aaccgcagcc tgccgctgcg atgccccagg
      301 ccaggaataa tctcctgcct gcggcttcgt atcccgccgt ataagggaag gaaaagaaag
      361 aagtgacggc cgccagaagg aggaaggcct cccaaaccct gtccatttcc gtatattgcg
      421 atgggacggg catgcccgca aacggaatga ggagggcgta cagggcgggc aggaaggcca
      481 gcatgctcag atttcttgtt attctgcttc cgcgggaggc cagcaggact ctggcgcccc
      541 agaacatgag gaaggacagg gaaaggatgg agagcaattc cgcccctgtg cccaaaagca
      601 ggaagggtgc ggctgtccgg ggagagcgga ggaaaaaagg catgaacaga tggatgatga
      661 ctgccgctgc tgcgcccgcg ccgaaaagaa cggcgcagac tttgatgaaa gccctggctg
      721 ccgggtaaat catccagttc atggtgaaga gaaggagtag aggggatggg aaaaaggaaa
      781 aggaccgctc cctttgcgga agcggtcctg aggggttagc gtgaattcat ggatgccaat
      841 gcgaatgacg gcatgcggaa ttacttatac tgggcccagt cgacattctt catgtctccg
      901 cttctggcaa gttcggcgtg catgcccaaa gcggccctga tggattgcgg tgtctgggct
      961 ttgccgtcgg acagcttcag gtaatcccag aggatgtttt tgtaatccgg gtggacgcag
     1021 ttttcaataa tggtctgggc ccgttctttc gggctcttgc cgcgcaggtc cgcgaccccc
     1081 tgctcggtaa taatgatatt gacgtcatgt tccgtatggt catggtggga aaccatgggg
     1141 acgatagcgc tgattttccc ttccttggcc acagacggac aagtgaagat agagatataa
     1201 gcattgcggg tgaagtcgcc cgagccgccg atgccgttca tcatcttcgt gccgccaatg
     1261 tgcgtggagt tgacgttccc gtaaatgtcg gcttcaatgg cggtgttgat ggagatgacg
     1321 ccgagacggc ggataacttc cgggctgttg gatatttccg agggacggag caccagtttg
     1381 tcgcggaaaa agtccatatc cctgtaaatg ccttccaggc agtcgttcgt cacggtcagg
     1441 gagcaggcgc ttccgaattt cacgcggccc tcccgaatca ggccgatgac ggagttctgg
     1501 atgacttccg tgtacatctc gaacgcggga atcgtcttgt cgcggcccag ggcgcccagc
     1561 accgcgtttg cgatgttgcc tacgccggac tggagaggca ggaacgtgga gggaatgatg
     1621 ccccgtttca tgtcggcagc caggaaatcg gccacgttct gcccgatttt atcggtcagg
     1681 ggatccgctg cggcgaagga acgggcttca tccggccagt tggtttccac aacgcccaca
     1741 atctttttcg ggtcaacctg gatgtacggc ttgccgatgc ggtcgctggg tttgaagatg
     1801 gagatttcgc ggcggtaggg aggatccagg ggttcgtaaa cgtcgtgcag gcccatggcg
     1861 ctcttgctgt gggcggcgtt taattccacg ataattttgt ctgccaggcg gcagatggtg
     1921 ggagcgatcc cgcccgcggc cgtcaggtaa atttttccgt cggccgtgac ttcacaggct
     1981 tcaataatgg cgatgtccac gttgcccatg aacccgtagc gcacttcctg ggccatctgg
     2041 gacaaatgga tgtcattata ggcgatttcc ccgttgttca ccgccttgcg gaaatcaacg
     2101 ttggtggtgt agggagcgcg gtagcggatt gccttggccc gggaaaggat cccgtcgcag
     2161 gaatcgccgg tggaagcacc ggtgaagacg ctcacctgga aagggttccc cttctcatgt
     2221 tccgcttctg cgatttttgc cagcgcagcc gtaacggcct tggccgttcc ggcgggcgtg
     2281 aatccgctca gcccaatatg gtggccatgc ttgatcaggc ttgctgcttc ttctggtgtc
     2341 agtaccttgt aactcatgtc agtgattttt gttggatgaa aaaagaaaga aaccccgtgt
     2401 aaaacggagc ggcaggctca ttcttcgagg agcctgttga tcaggtgcgc cggaatggtg
     2461 cggacggtgc cggccggaag ggatagcaca ggattgccct gatcgtcaat ccctgtaaaa
     2521 atgccccgga cggtttcgtc atccgtaaga atggaaaccg gcctttctcc ggcccatgcc
     2581 cgggacagac cctcctgaag ggcggggacg ccgccgtggg aaaagacgga aagaacctgg
     2641 ttcaaatgac tgcccaggga agccatgacg gcgtgcaggg aagggcagtc ccgaacaaga
     2701 tccgccagcc ttgccggagg gtccttgatt tccccggcga tggaggatac atcattgaaa
     2761 acattgatgc cgatgccgat gacggccatg ttctccgccg ggcgttccac cagtattccc
     2821 gccagtttgg atttgcccgc cagcaggtcg ttcggccagc gcaggcgcac ggaatccagc
     2881 cccatgccgc ggagcatgtt gaacagggcc agccccgcaa ccaggggcaa atggccccag
     2941 tgcacgccgg ccgctgtatc ctcaggagcg gacaggggga cggtgaagga tgcccacagg
     3001 ccgccttcct cccctatcca tttgcggttg aacctgccgc ggcctcctgt ctgaacgcgg
     3061 cagacggcaa tatgccccgg ggggagggct cgggccagat cattggtgga aaaagcctcg
     3121 gcccattcat gataagtcca gtcgccgcat ggtccttcct cctccggagg ggtggaaaat
     3181 gaggctgcgg aattcatggg acgggccgag gccgggagta attttcaatt ctggttattt
     3241 cgccagagtg gcgatgtagt agcccgcgat gactgcggtg ccgatcacgc ctgcaacgtt
     3301 cgggcccatg gcgtgcatga gcaggtagtt ggaggggtcg tacttctggc ccacattgtg
     3361 ggagacgcgg gctgccatcg ggacggcgga gacgcctgcg gacccgatca gggggttgac
     3421 cggattcttg cgccagatga ggttcataat cttggcggca atcactccgc ccgctgttgc
     3481 cacgccgaag gcgacgatac ccagaaggat gatcagcagc gtttccgact gcaggaagcg
     3541 gtcgccctgc atcgtgagcc ccacggaggt tcccaggaag atggtgacga tgttgatgat
     3601 ttcattctgg gaggcctgaa ccaggcgttc cgtcaccttg cattcgcgca ggaagttgcc
     3661 aagcatcagc atgccgatca gcggggatgc gtccggaatc agcaaaatgg tgacaatggt
     3721 taccagcacg gcgaaaaaca gtttttcgct cttgctgacc gttcgcaggg atttcatgcg
     3781 gatgaggcgt tccttcttgg tcgtcagcaa cgccatgatg ggcggctgta tcagcggaac
     3841 cagggacatg tacgtgtatg cggccacggc cacggcgccc agcaggtgcg gggccagctt
     3901 catcgtcagg aagatggagg tggggccgtc agcgccgcca atgatgccga tggacgcgga
     3961 ttcattcggg gtgaacccca tgaacagggc catgaagaac gtggcggcta cgcccacctg
     4021 ggccgctgcg cccagaagca gggtgcgcgg agcggcaagc agcgggccga agtccgtcag
     4081 ggctcctacg cccagaaaga tgatgggcgg gaaaatttca gccttgatgc ccagcccgat
     4141 ccagtcatac aggcccccgt ggatggaggt gaccagcagt tcctgggtat gctggcctac
     4201 ggccacgtat ttgcctgcgg ctgaaaccac caggggctcc agcttctggg ttttcagatc
     4261 caccggaatc cggttgccgc tggtgtcaaa gtaattgacg ccctgctggc ggacgacggt
     4321 ggtcccttct ccgttcgtga gcaggaagac cggttcattg ccgttgtcct ccgtcacgtt
     4381 ctggaaatcc ttcaggttgg ggtccaccgt caggctgtcg tcgcccagga gggccgcgtc
     4441 agccttggct ttttcctggg attcccgcac ggacttgatc ttggaaacgg tcagctggct
     4501 gccggggtaa acctgcatgg gctgctgcat gatttcctga tactgggcac gggattcagg
     4561 cgtggtcagg ttggccggca ccttggaggg agcctgctga agcggagcca cgtgaacgcg
     4621 caggtacccc acgttgttca gagacgtggc ggttacttcc ccctgttcat tggaggagat
     4681 gacctgctgg ttcagctggg tgatgagcat tccgttatcc ggaatgttgg caatgagggc
     4741 gccgaaagca atgggaacca tcaggagcgg ttcgtattgc ttggctacac ccagatacag
     4801 gaggagaatg gcaatgcccc acatccctac catctgccat gatagggaga agacgcccat
     4861 cccctgtaaa aaggtgatta atgaatcaaa aagactcata gggaatattt ttgtagcggt
     4921 ttattatctg agccggagcc cgggactgtt ggcgtttccg ggctgccgcc gccgggatta
     4981 accaattttg gccagtgcct gaccttcctg aaccgtgtcg cccgggttca cgcagaaagc
     5041 ggtgagcgtg ccggaggcgg gagcgtagat gatggtgttc atcttcatgg cttccagcgt
     5101 cagaatctgg tcaccggcct tcacgggggt gcctgctgcc acgtccaggg agacgacctt
     5161 cccggcaagc gggctgggaa cgtctcctgc gccggcggcg gccggagccg gcgcaggtgc
     5221 gggagccggg gccggggcgg ccacgggggc ggaagccgca ggagcgggag cggccacagg
     5281 tgcgggggcc ggcgccgcag tggtggagga cacctggtcc agaagttcta cggatacgtc
     5341 aaatactttg ccgtctacgg tgatgcgaag tttcttcata atagtgtgtg gtatgggata
     5401 ttaagttaaa tgattccgca tgggcggggt aagggtgaat aatgcagtag ttcagcggga
     5461 aggcctgatg cggtgggagg cgaaaatctc cgccctgccc gcggccgcat agccggaacc
     5521 cggggattgc ttgatttcca cgatgcggtg gctcatgccg tccaggcctg cgtccacggc
     5581 ggcggagatg acggccacca tttccggagt aagctccgtc atggcggaat ccacggcggc
     5641 ggaaaggatg gcgagcattt ccggagtcat gcccggtttg cccgccgcag cgggagcggc
     5701 ggcaggcacg gctacgggag cggcggtttt ggccctgttc tgaatggcgg cggcgatgga
     5761 gccgctgacg gtcagaatga cggccatgcc tcccaggcac agcataacca ccatgatgcc
     5821 gacaacctga tagttcagat tgttcataac gttggcgatg ccctgtgcga aggcaaaagc
     5881 gagtgttgta agtaacatat gaatccgttg ggatggatgt tagagcggaa tgttgccgtg
     5941 tttcttggcc gggcgcactt ccttcttgtt cagcagggtg cgcagggcga aggcgatttt
     6001 ggcgcgggtt tcttccggat tgatgacttc cgtgatctgt cccatgccgg ccgccacata
     6061 cgggttgtaa aaggcttccc ggtattcttc cagaagttct ccctggcgct tggctttttc
     6121 cgccgggtct tccacagcct tcagttcgcg gccgtagagt accgggacgg cgccttccgc
     6181 acccataacg gcaatttccg caccaggcca ggcgaagacg gcgtcagcgc ccaggtcctt
     6241 gcagcacatg gcgatgtagg cgccgccgta ggctttccgc atgatcaggg tgactttggg
     6301 caccgtggcc tgggaatagg cgaagataag tttggcgccg tggcggataa tgccgccccg
     6361 ttcctggttc ttgccgggca ggaagccggg cacgtccacc aggttcacca acggaatatt
     6421 gaacgcgttg cagaaacgga tgaagcgtgc ggccttgtcg gaggagtcga tatccaggca
     6481 gccggctttc acattgggct ggttggcgat gatgccgacc accacgccgc agatgcggcc
     6541 gaatccgacg acgacatttt tggcgaagtc cttgtgtact tccaggaagt ccccgttgtc
     6601 caccagacgg ctgatgatgg gctggacgtc cagcggcgtg cggttgtcgc cgggaatcag
     6661 gtcgctcatg ccttcgtcag cgctcaggtc cagcggagtg tccagtttgt ggggcggttc
     6721 ttccgtgttg ttggaaggaa ggaaggaaag aagcctcttc aggatgtcca tggcgtgcgc
     6781 gtcgctgtcc gctacgaaat ggacgttgcc ggacacggtg gcgtgaatgg cggcggagcc
     6841 gatgtcgtcc atcgtgcatt tttcatacgt gacctgttca atcactttgg gaccggtgat
     6901 gtacatgccc gcgttgccgg aattgcgcat gatgatgaag tccgtcaggg cgggggagta
     6961 ggccgcgccg cccgcgcagg ggcccaggat catggagatt tgcggaacca cgccggaggc
     7021 aagcacattg ttgtaaaata cctgggcgta tccggaaagg gccgccactc cttcctggag
     7081 gcgcgcgccg ccggagtcgt tcacggtcac catcgggatg ccggctttca gcgcatactt
     7141 ctgagcatcc gcaattttca tggcgtgcat gtagccgagg gaaccgccct gggccaggaa
     7201 atcggaggcc gcgcaggcta ccgggcggcc gtccaccagg ccgaaaccgg aaacgacgcc
     7261 gtcgccgggg atttccttct tacccatgcc gaagttgtgg cagttgtggc gcacatgcat
     7321 gccgatttcc gagaaggtgc cttcctcaaa gaggtacccc atgcggtcgc gggctgtcat
     7381 ttcgcccttt tcatgtctct tgtcgatctt ttcctgaccg ccgctgagga tgaccttctt
     7441 gcggcggctg ttcagatcgt caatcaattt gggatcaata gccattggtc tagtggtatg
     7501 gtgaataggg agaatgttac ttgccgcatt cgcagctgcc ggggtgctgg cagaattcgg
     7561 tcaggacgcc gaaggtgcac ttgggatgca ggaaggtgac ctgcgtgttg tgagcgccgg
     7621 ggacgggcgg gtcgttcagc acggtaaggc ctgcttcctt ggcctgggag atatttccgg
     7681 cgatgtcatt ggtcttgaaa gcgatgtggt ggatgccttc cccttttttc tcaatagcct
     7741 tggcgatagc gctttccgga gaggtgggtt cgagcagttc gatgtggact ccggcaacgt
     7801 caaacatggc gacgcggacc ttttgagtgg gaacttcctc aatgtggggc tctccgagcc
     7861 cgagcgcatc gcggtaatag gggacggtgg cgtcaaggct cttgacggcg atgccgatat
     7921 ggtcaatttg ctgattcata tgcgtgtgat ggttataggg aatgcagggg ggaggcctgc
     7981 aatacatcaa gagttattaa agcgtatttt tttaggatgt gaagcgaaaa gcggaaaaat
     8041 ggcgaattcg tggccgtccc cttcacctgc ttgattgccc cggaagatga ggtatggtag
     8101 gggcatgttc aagagtggat tagccgtgtt atttctgctg gcgtcgtggt tttccgccgc
     8161 gtgtgcctat gagctgctgc ccgctcatgt ggccgtggtt tataacggaa aatccgagct
     8221 gagccgccgc atggccaggg agtatgcgcg ggtgcgcggc gttccggaag ggaatctcgt
     8281 ttccctggat tgccccatga ccagcgagat ttcgcggaag gaatatgagg atacgatccg
     8341 cgtcccgctg ctggaggcgg cgcggaaaca acggtggtgg gtgccttccg gcattgcgtc
     8401 ctcccccttg atgaaccgga agatttttgt gctggtgctg atggcggacc tgccgatgaa
     8461 gatacggcat gaaacgcccg ctccccttcc cgggaaaggc gtcaaccaga tgcagacgga
     8521 tcgcgctgcg gtggattccg aactggctct gctggcggtg ggcggttatg aaaggaagtc
     8581 ctggcaggtg aatccctatt ttaataaaag ggaggatttt gtgggttccg gccttccttc
     8641 gtttctggta tgccgcctgg acggactgac gcccgatacc tgcatgcggc tggtggcgga
     8701 accggcgaac gtggagaaaa aaggtttatg ggggtgggcc agggggaccg gtggctggac
     8761 gacgtgttcg agcgcgtgag ggaggcgggc attcccgctt atctggatga ctggccccag
     8821 accctgccgg aaaaattccc cttgagccgg gatacggctc tttactgcgg gtggtatgcg
     8881 gggaatgcga acggaccgtt ttcggatcct tcgttccgct tccggcccgg ggcgatagcc
     8941 atgcatctgc attccttcag cgccgccgat ttcaaggtcc ccggcagggg gtggagttcc
     9001 gccctgctgg agaagggggc tgctgtgacg gtgggaaatg tgtatgagcc gttcctgggc
     9061 gcctgccacc gctttgatat ttttgtggac cgcctcctgg acggctatac cgtggcggag
     9121 gcttcttgga tgagcatgcc tgtgctgtcc tggcaggggg tggtgttcgg agaccccctg
     9181 taccgtccat acgcccgcat gaaggatatg gacgtggagc cgacggaaga agaccgttat
     9241 ttccaggggt ggtgggcttc ctccgtgcag ttcggagacc gctggaagga ccgttccgcg
     9301 cgtttgatgg agtccgcccg gaaggcccct ttttcctgcc tttatgaagc tctggcgctg
     9361 gaatgcctgt accggaagga accggcccgc gccggagagc ttctttcctc cgccctggac
     9421 ggcgcggctg acgcccgcac ccgcgcgcgc ctgctgctgg aaatcctgat ggcggaacgg
     9481 gcccggggcg gcaacaaggc ctttctgcag cgggcggacg gcatccgggg cctcatgtct
     9541 tcctccgctt ttcttcctgc gctggaggaa tggcttgcca gggtggctcc gccgccggcc
     9601 ccgccgaaaa aataaattaa ggacgtacgg caaatttgga ttgcctttta ggggattatg
     9661 gctcatattc tgcgtcccgt aagttttcct tttactgata ttcaccagca atagataaca
     9721 caagatgaac attatcaaca aaatcgagca agagcaactc aaggcggatg tgaccccatt
     9781 taacgtaggt gataccatca aggttcacac tcgtgttatt gaaggcggca aggaacgtat
     9841 ccagattttc cagggcattg tgatcgccaa nnnnnnnnnn cggttccggc atcaatgaag
     9901 ctttcaccgt ccgcaaaatt tcctacggtg aaggcgtgga acgcgtattc cccctgcata
     9961 ctccccgcat tgccaagatt gaagtcgtga accgcggcaa agtccgccgc gccaaacttc
    10021 actatctgcg cggccgcatc ggcaaggatg ccatgattgt gaagtctgcc aaccggtaat
    10081 cggaagaatt gctattttgc tggcccggcc cgccgtgagg cggcgccggg ttttttattt
    10141 ccggagtatt gtttttcctg aatgggaaag gggtccacag ggacgacagc cgcttcagga
    10201 tatttccccg ggagccatat ggggatgaga gcgtttttcc ggggtgggga aggtggagaa
    10261 gggcggctgt ttccggtccg gagcaaatac gtttttcgga ttttcctccg tccgggcctg
    10321 tatggctccc ggagaatgga gcagaaaccg gaaaatttca tacgcgaagc caatcccgga
    10381 acttagcgcg tgacattcca tcaggccctg tcccgtcctg cttttccggg aaggggaaag
    10441 gaaacggtag ttttcagcat ggcccccgga ggggggtaag cgggagcagg gctgcgcgga
    10501 taattatttt tccgcaggtg cgggagccgg tgtgtcctcc gcatcttccg gtccgtacgc
    10561 ttccacttcc gtcaacgcca gcggctggga gggatttgcg gattccacgc gcagggcgcg
    10621 cgccttgacc ggagcgtcca gtttccagga agtgacgttg gtggattttt ccgaaggggt
    10681 ggtgctgaag gttttttccg ccagcacgtt tttgtcattg ccgtagagga ttaccttgaa
    10741 tttttccatg gtgcccagca gcgtcaggtt ggccggcgtg taaatgacca ccttttctat
    10801 ggtgcggttg gtctccctgc cgaagtcaat gccgaaccag gcggtttttc cctcaaccac
    10861 gcctgtagag ctgattgaaa cgtcggaaat cctggagctg gtattgccgt caacggcctt
    10921 ttccggaccg taagctccca gcatgttgcg gttggaactc tggcgaacct tgacgccggg
    10981 ttgaaggctc caattgactt tgccgggttc cgcggaaact ttgggaactg ccggcgcctg
    11041 cgccggacgc gcggccggaa caggtgaagg aatggccgca gcggcgtttt gcgcggtggc
    11101 cggagcgtgg cggcgcgcgt ccctgttcgc ttgtcttgcc gcttccgctt tcgcgttatt
    11161 ttccttttgc acctgttcat tataatcatt gcgcattcta acgacatcct tcttatacag
    11221 gatgcaggta taggtcagca caatgcccac gatcagggcc ggaatgagat agtaatagat
    11281 gttggactgc cctttttgca cgttcagggc cggaatggcc ggggaagatg gcggcggagg
    11341 gatggaggcc gcatttcttc ccgcaggatt ggcaatggct ttatccagat ccgtgatgaa
    11401 atcccccagg tcccggtagc gttcccgggt atccggattg atggcgcggg agactgccga
    11461 atccacggca tcgctgcatt tgattagtga ggagggcatg gcaaagcccg cttccatcgg
    11521 cgttttgcgg gtaagcagaa tgtaaagcgc cagccccagt gcgtaaatat ccatgcccgg
    11581 cgtgaacgtg gtttccgctc cggtgaccca ctccggagag gcgaagtccc ccatgtccac
    11641 cggaggttgg gcgggataaa tgttgatggg cagcagcagg gcgtctccgc cgtcttcgcg
    11701 caggcaaatc agcttgggcg tgatgccccc gtgaaaaatg tttttccggt gcagcgtcag
    11761 cagtccttcc gccatgttgc gcaccaggtt gagcgccgcc tggggagata tttggggaag
    11821 ggtttccata tccgccagac gcggataggg ggagtgttcg gagatgatga aagtaaacgg
    11881 ccccgcctgt cctacttcat aaacgcgcag gagcccctgg tgggattgtt ccacgatggt
    11941 gagggagcgg acgatgnnnn nnnnnngatg ccggcttctt ccgtgggaac cacccgcagc
    12001 ataacgggag caaaatttga tttcagggta gctgtgaaaa tttgctgggg gcctttggct
    12061 ccgacaagct ggatattttc caattgggga aagagagagg aaatctggtc catgggaaag
    12121 gaatcgtcgg cagacatgga aaagggaggt ggaatagtat tgattcccac tctaccttaa
    12181 attcccctac ggaagcaact ctataagcgg gcatattttg atgtcgggac gccctgatgc
    12241 tgaacggacg ccaatcatgc ccggatggag cgcgcccttt tccctaaccg gccagtcaaa
    12301 tcagggcagc caggaacagg ccgtgatgag cagggaaaat ttcagttgtt tcaactgcgg
    12361 cggcatgttg ccgcagatgc ccgcggcaag gtaaatcctg tccggttcct gtttttcccg
    12421 gaagcggcag ggagggcgga tgaacgaggg cgctccagcc gttaaagccg ggacatacag
    12481 gaacccggaa actggaccgc cgaggcgtgg ccgtttggct ggaagatggc tgccgctatc
    12541 agcaggaaca gcagaagggg gaagggaagg aggccttgca tggacaagga gaatcagccc
    12601 cctgcatcag ggccttttca ggatgatagg cggcgaggga ttggcctgcc atacgggagt
    12661 ggcggctttc gcgtccgctt cttcgagcgt tttacggcct ttgaccttga ccaggtgttc
    12721 ccggaggatg cgccgcagtt ccacggcgga ggccgggtca tcctgcacgc tgtgcccgga
    12781 cggaacgatt ttttcactgg cgctccagtc caggtgggat gagtg
//
