LOCUS       c00182_gnlPro..         8112 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|MFCGALEC_182.
ACCESSION   c00182_gnlPro..
VERSION     c00182_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 12:04:34
            Original ID  :: gnl|Prokka|MFCGALEC_182
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 8112
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..8112
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[5499:8112]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      5500..8112
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..8112
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..8112
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(2..2869)
                     /locus_tag="ctg182_1"
                     /transl_table=11
                     /translation="MRFTRLILALTSFLILITQTDLFASTVLHKTSMVDLSNNAIICMH
                     QDPDGYLWIGTYDGLNLYNGKDTYVYRFELNNKNSLCSNIIHKISDAEPGFLWISTSLG
                     INKFSLKERKVTESFPGYMECDLLASDSSGITLAICKENRISCYTPSSDGFRDLPREGV
                     GPQTVRTLFAGTDGHFYLLLASGILKKLTPDFSDTTFNLLSEERQFHNKPILSAFYENN
                     HIFIVDTDNKLYRQHVDGKGKEFLFDLPEITKQYGNIIKICTFQSNVYIVFRNGNILDL
                     SQPENTINMGIGIFCLMNDKRQEILWLGTDGQGIRMFYDKPDLFGSILLKDLPINIQNP
                     IRSLYTDDDQSLWLGTKGDGIVRIQAYDTYHNKKMIPQSAITHFTTADGLSSNRVYCFQ
                     KSEYHPCIWIGTEGPGLTYYSYKEKRIKTIPQREDTTPLRYVHSICEVDDSTLWLATTG
                     NGLQKVTLHIDKAVPTIGKVQTFPLKNGKNICKEIQSMVYDNDSTLFLGSRGGYGVIRF
                     NIFNQGYEFLQTNNLRNPAIGDVLSVCQTEDSTFYAGASSGLTRIKFRGGKMRLRQFDK
                     SDGIVNDMIHGIHEGNDSCIWLSTNKGLTKYNPRNNFFHNYHQPYFSVTEFSDDAYWKC
                     PYSERLFFGGINGLVWVNKQTEPEHTYQPELSFFELQMDKQILPLYKDISRNGVTVPAD
                     VQSLTIAFVAPDYINGENYEYSYQLVNYNSSWEKLQKTNKVTFMNLPYGEYLLKVRYRN
                     DVIDSSAKEYTLPIKVLPPIYLSSLAIFTYLFIGTVLLIIATYRIHHQILKKQKQIADK
                     IKEEQKEKLYESKLNFFTHITHELCTPLTLINGVENYIQAYAATSKDKTLEKYTSVLRE
                     NVEELNGLIQEILDFRKAEDAGFSHTHIRRVSVSSLLRTQFEWFYPLSEQHQIQFKIDA
                     PKELYWNTDSVYFKKILANLISNAFK"
     CDS             3026..5329
                     /locus_tag="ctg182_2"
                     /transl_table=11
                     /translation="MEQCEENSQENDDVIIEKEISKLIEEQRLFTESIYACLPVGIEIY
                     DAHGVLRSINDHALRMYGVDDRTSVVNIVNLYKSPFVDSELLARIQSGEDISLEFEYDF
                     DRVNKDAYYSSHNKDTMIYGVKVIPIWSRKKHLIGHILLANDMTSVKEVEFRTEESKKN
                     LEMAMKAANMASWVYDVNKKVFNPLYGLTVAKKNMPMEAVLDILHPQDRAPLIRLFSQL
                     IGNEIEQGQMTLRFYNEQEEMYRYYESRMRLSTEHRGKLQIIGTQMDITERLQMAKKAQ
                     DLIAKRDLAMKVSNIVHWDFDVNSQKFESYNDPINDYVSDRLLTVSEYMDVIYPEDRSV
                     FYDAMQSLIAGKNVTINFTCRIQTKYDETWQYCDFMGVPFDQDENGDIIRYTGFRQNIP
                     KIQQLNRELKERNYKIELSFKTVGMSYWDFDVKNLKFSAFNDPINDFYSEKTITPEDYL
                     NATHPDDVVFVRKYMEHMFRGIDKDFNVKYRSKTKWDDEWQTLLVTGIQVERDKEGHVT
                     RYTGITINNTKWEKMIQQLKELKEKAELSDRLKSAFLANMSHEIRTPLNAIVGFSELMV
                     TCDDPEEKKEYINIIQSNNELLLRLINDILDLSKIESGIIERRKENFNLAKVCNELFVT
                     IQAKMTNPNVELRLDGPNSECWVLLDRNRLKQVWMNFLTNAVKCTKSGYIKMGYGIERK
                     GLRIYVEDTGIGIPDDLHDKVFTRFEKLNEFSQGTGLGLTISRAIVEAAGGEVGFTSKL
                     GVGSTFWAWLPLRP"
     CDS             5500..7740
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg182_3"
                     /sec_met_domain="Gly_radical (E-value: 1.6e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.2e-282, bitscore:
                     926.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             7741..8112
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 69.3;
                     E-value: 1.3e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg182_4"
                     /sec_met_domain="Radical_SAM (E-value: 2e-25, bitscore:
                     78.3, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLQYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLV"
ORIGIN
        1 acttaaatgc gttggaaatc agatttgcta ggatcttctt gaaatagacg gaatccgtat
       61 tccaataaag ctctttggga gcatcgatct tgaattggat ttggtgttgc tcggatagtg
      121 ggtaaaacca ttcgaattga gttcgcaaaa gagaggatac ggatactctc cggatgtgcg
      181 tatgactgaa tcctgcgtct tctgctttcc ggaaatcaag gatttcttgg ataagtccgt
      241 tcagttcctc tacattttcc cggaggactg aagtgtattt ttctaatgtc ttgtcttttg
      301 aggtggcggc ataggcttgg atataattct ccacgccatt gatcaaggta agcggggtgc
      361 atagctcatg cgtaatgtgg gtaaagaaat tcagcttgga ctcgtatagc ttctcttttt
      421 gctcctcctt gatcttgtct gcgatctgct tctgtttctt tagtatttgg tgatggatac
      481 gataggtggc gatgatgagc aaaaccgtac cgataaaaag atacgtgaaa atggctaacg
      541 agcttagata aataggaggt aataccttga taggtagagt gtattctttt gctgacgaat
      601 caatgacatc gttgcgataa cggactttca gcaaatattc cccgtacggt aggttcatga
      661 aagttacctt gtttgtcttt tgcaattttt cccaagagga attatagttg accaactgat
      721 acgaatactc gtaattctcc ccgttgatgt aatccggagc gacgaacgca atcgttaacg
      781 attgcacatc agccggaacc gttacaccgt tccggctgat gtccttatat aatggaagga
      841 tttgtttatc catttgcaac tcgaagaaac ttaattcggg ttgataggta tgctccggct
      901 ccgtttgttt atttacccag actaatccgt tgatgcctcc aaagaataaa cgctcggagt
      961 atgggcattt ccaataagcg tcatcagaga attccgtgac gctaaagtaa ggctgatgat
     1021 aattatggaa aaagttgttc cggggattat atttggtgag acctttattg gtactaagcc
     1081 agatacagct atcgttcccc tcatggatac catggatcat gtcgtttacg atcccgtcgc
     1141 ttttatcgaa ttggcgtaaa cgcatctttc cccctctgaa tttaatccgg gtgagaccgg
     1201 agctggcgcc tgcataaaac gtggaatctt cggtctggca tacggaaagc acatctccaa
     1261 tggccgggtt tcttaaattg ttggtttgca ggaattcata tccttgattg aaaatattga
     1321 aacgaatgac tccgtaaccg ccacggcttc cgaggaacag ggtagagtcg ttgtcatata
     1381 ccatcgattg gatttcttta cagatgttct ttccgttttt taacgggaag gtttgtacct
     1441 tcccgattgt aggtacggct ttatctatat gtaaggtaac cttttgcaat ccgttacccg
     1501 ttgtcgctag ccataatgtg gaatcatcta cctcgcaaat agagtggaca tagcgtagag
     1561 gggtggtgtc ttctcgttgc ggtatcgttt ttattcgttt ctctttatac gaataatagg
     1621 tcagtcccgg gccttccgtg ccaatccaga tacatggatg gtactcgctt ttttggaagc
     1681 aatacacccg gttgctggat aggccgtcgg cggtggtgaa atgcgtgata gcgctttgtg
     1741 ggatcatctt tttgttatgg taagtgtcat acgcttgtat ccggacaata ccgtctcctt
     1801 ttgtcccgag ccaaagggat tgatcgtcat ctgtatataa agaacgtatg gggttctgta
     1861 tattaatcgg taaatccttg agtaagatag agccgaaaag atcgggtttg tcataaaaca
     1921 tccgtatacc ttgcccgtct gttcctaacc agagaatctc ctgtctttta tcgttcatca
     1981 ggcaaaagat accgatgccc atatttatcg tgttttccgg ttggcttagg tctagaatat
     2041 tgccgttacg gaaaacgata tacacattgg attggaaggt gcatatcttg atgatgttac
     2101 catattgttt tgttatttca ggcagatcga acaggaattc tttccccttt ccgtctacat
     2161 gctgtcgata taatttattg tcggtatcta ctatgaaaat atgattattc tcgtaaaatg
     2221 cggataagat cggtttatta tggaattggc gttcttccga tagcaaatta aaagtagtat
     2281 cggagaaatc cggagtcaac tttttcagta tgcccgaggc taataataaa taaaaatgtc
     2341 cgtccgtccc ggcgaataag gtacgtacgg tttgagggcc tactccttct ctcggcaggt
     2401 cccggaaccc atcgctagat ggggtgtagc aagagatcct gttttcctta caaatagcga
     2461 gcgtgatgcc ggatgaatcg gacgctaata gatcgcattc catgtatccg gggaaagact
     2521 cggtgacttt ccgttctttt agtgagaatt tattgatacc taaagaggtt gatatccata
     2581 aaaagccggg ttctgcgtcg gatattttgt gaatgatatt actacaaaga gagtttttgt
     2641 tatttaattc gaaacgatat acatacgtat cttttccgtt gtataagtta agtccgtcgt
     2701 aagtcccgat ccacaaatag ccatccggat cttgatgcat gcagataatg gcgttgttcg
     2761 agagatcaac catagaagtt ttatgcaata cggttgaggc aaataggtct gtttgcgtga
     2821 tcaatataag gaaactggtt aacgctagaa taagccttgt gaatctcatg tatttaagat
     2881 ttagtgtttg ttttcagcgc aaagataata tctttatgaa cgaacagaaa agaattcact
     2941 tttttgttta cttttgcaaa aagcagggct ttcgtataaa ttagaatgtc tgacgtatga
     3001 taatacgaaa taaagtaagt gacagatgga acaatgtgaa gagaattctc aagaaaatga
     3061 cgatgtgata atagagaaag aaatttctaa attaatagaa gaacagcgtt tatttacgga
     3121 gagtatatat gcttgtctgc cggtgggtat tgaaatttat gacgcacatg gtgtgctgcg
     3181 tagtataaac gaccatgcgt tgaggatgta tggtgtggac gaccggacat cggtggtgaa
     3241 tatagtcaat ctttacaaaa gtccatttgt tgattcagaa cttttagctc ggatacaatc
     3301 gggtgaagat atttccttgg agtttgagta tgattttgat cgggttaata aggatgcata
     3361 ttattcttcc cataataaag acacgatgat ctatggggtt aaagttatcc cgatatggag
     3421 tagaaaaaaa catcttatcg gacatatatt actcgcaaat gatatgactt ccgtgaaaga
     3481 ggtggaattc cgtacggaag aaagcaagaa aaatctggag atggccatga aagcggctaa
     3541 tatggcttct tgggtatatg atgtgaataa aaaggttttt aatccattat atggactaac
     3601 cgtagccaaa aaaaatatgc caatggaggc ggtactggat attttgcatc cccaagatcg
     3661 agctccgtta atccggctct tctctcaatt gataggaaat gagatagaac aaggacaaat
     3721 gactttgcgt ttctataacg agcaagaaga gatgtatcgt tattatgaga gccggatgcg
     3781 cctatcgaca gaacatcgtg gaaaattgca gatcatcggg acacaaatgg atataaccga
     3841 aaggttgcaa atggccaaga aagcgcagga tctgatcgca aaacgtgatc tggccatgaa
     3901 agtcagtaat attgttcatt gggattttga tgtaaattcc cagaaattcg aatcttataa
     3961 tgatccgatc aacgattacg tttcagatcg gttattgacc gtttcggagt atatggatgt
     4021 tatatacccc gaggaccggt ctgtttttta cgacgcaatg caatccctga tagctggaaa
     4081 gaatgtcacc atcaatttta cctgtcgcat acaaaccaag tatgatgaga cttggcagta
     4141 ttgtgatttt atgggtgtcc ctttcgatca agatgaaaat ggagatataa tacgctatac
     4201 gggattcagg caaaacattc ctaagatcca gcaattgaat cgggaactta aagaaagaaa
     4261 ttacaagata gagctttctt tcaagacggt tggtatgtcg tattgggatt ttgatgtaaa
     4321 aaacctgaaa ttcagtgctt ttaatgatcc tatcaatgat ttttattcgg aaaagacaat
     4381 cactcccgag gattatttga acgctacaca tccggatgat gtggtttttg tgcggaaata
     4441 tatggagcat atgttccggg gcatagataa agatttcaac gttaaatatc gttccaaaac
     4501 caaatgggat gacgagtggc agaccttatt ggttactgga atccaggttg aaagagataa
     4561 agaaggccat gtgactcgct ataccggtat tacgatcaat aatacgaagt gggagaagat
     4621 gattcagcaa ttgaaggaat taaaggagaa agcggaactt tcggatcgtc tcaagtctgc
     4681 gtttctcgct aatatgagtc acgagatacg taccccgtta aacgctattg ttggtttttc
     4741 agagttaatg gtaacttgtg atgatccgga agaaaaaaaa gaatatataa atatcatcca
     4801 atccaataat gagttgttat tgcgattgat caacgatatc cttgatcttt ccaagataga
     4861 gtcaggcata attgaacgta ggaaggagaa tttcaatctg gctaaggttt gtaatgagtt
     4921 atttgttacg attcaggcga agatgacaaa tccgaatgtc gagttacgtt tagatggtcc
     4981 caactcggag tgctgggtac ttctggatag aaaccggctt aagcaagtat ggatgaattt
     5041 cctcactaat gcggtaaaat gcaccaaatc cggatatatt aaaatgggat atggcattga
     5101 aaggaaaggt cttcgtatct atgttgagga tacgggaatc ggtataccgg atgatttgca
     5161 tgacaaagta tttacccgtt ttgagaaatt gaatgagttc tcgcagggta ccggacttgg
     5221 cttgacgatc tccagagcta tcgtagaagc ggcaggggga gaagtgggct ttacttcaaa
     5281 acttggtgtc ggatctactt tttgggcatg gttgcctctt cgcccttagg tctgactccc
     5341 catttattgg taatgtcctt ttaaaatagt gaaggatgag aagctttttt atcgcaattt
     5401 atagggattt tagaactggt gtagcatatg tgacagaaat atgacgataa ttctcctacc
     5461 tttgcggcgg taaaaatgtg gtattagata agattgttta tgaaatttga gaatgagtgc
     5521 tgggatcact ttaagggtga gacctggaaa agagagatca atgtaagaga ttttatccag
     5581 agtaactata cgccttatga aggggatgat tcctttttag tcgcttcttc cgagaagacc
     5641 cgtaaggtct ggaacaagct gaccgagatg tttaaggtcg agagggaaaa gggagtttat
     5701 gacgccgaga caaaattacc tcaagggatc gatgtatatg gcccgggcta tattgataag
     5761 gagaatgagg tgatcgttgg gttacaaacc gatgctccgc tgaaacgtgg tatcttcccg
     5821 aagggcggta tccgtatggt tgagaatagc ttggaagctt atggttatca tttggacccg
     5881 atgacgaaag agatcttcac gaagtaccgt aagacgcata acgagggcgt tttctccgca
     5941 tatacggaag agatgattgc cgcacgtcgt tcggctatta tcaccggtct gcccgacgca
     6001 tatggtagag gacgtattat tggtgactat cgtcgtgtcg ctctgtatgg tacggcaatc
     6061 ttgatcgagg agaaaaagcg tttcttgaat cgtttggata tccaagagat cacggaagag
     6121 atcatccaga gccgtgagga gatttccgaa cagatcaagg ctttgaaagc gttcgagaaa
     6181 atgtgcgcta gctatggctt tgatgtgact cgtccggcac aaaacgctcg tgaggcagtt
     6241 cagtttgtgt acttagctta tctggcggcg gtaaaagacc aagatggcgc ggcgatgtct
     6301 atcggacgta cttctacgtt tttggatatt tatatcgaga aagacatccg tgagggtaag
     6361 ttgaccgaag aggaggcgca agaattggta gaccagctga tcatcaagtt gcgtatcgtt
     6421 cgtttcttac gtacgccgga atacaatgat ttgttctccg gtgatccggt ttgggtaaca
     6481 gagtccttgg gcggccaagg agtggatggc cgttctttgg taacaagaac ttcctatcgt
     6541 tatctccata cattatataa tctaggtccg gcaccagaac ccaatttgac ggttctgtgg
     6601 tttaagaacg ctcccgagaa ctggaaacgt ttctgtgcca aggtctctat cgatacgtcg
     6661 gctatccaat atgagaacga cgatttgatg cgtccggatt atggagatga ttatggtatc
     6721 gcttgttgcg tttctcctat gaagatcggt aagcagatgc agttcttcgg agcccgtgcg
     6781 aatttggcga aatgtttatt atatgcgatc aacggaggac gggatgagcg cagcggcgta
     6841 caggtagccc cgatgttcga gccggttcgc ggcgagtatc tggaatacga tgaggtgatg
     6901 gctaagtatg agcaaatgat gcgctggttg gcgaaagtgt atgtgaacgc cttgaagata
     6961 atccattata tgcatgataa atacgcgtat gaggcattcg agatgtcgct tcacgatggg
     7021 gatgtagagc gtatccgtgc gacaggtatc gccggtcttt ctatcgttgc cgattcgttg
     7081 gctgctatcc gtgatacgaa agtacgtgtg atccgtgacg agcgtggctt ggcggttgat
     7141 ttcgaacgtg agggcgaata tgttcctttc ggtaataacg atgatcgtac ggatagtatc
     7201 gctgtagaga tcaccgagaa gttcatggaa tatttgcgtc agcacgagac ctatcgccat
     7261 gcgaaaccta cgcagtccat ccttacgatc acctctaatg tggtatatgg taagaagact
     7321 ggtacgacac cggatggccg tatcggtgga actcctttcg ctccgggcgc gaaccctatg
     7381 aacggtcgtg atacgaaagg tgctatcgct gcgctagctt cggtagcgaa gcttccattc
     7441 cagcatgcgc acgacggtat ctcttatact ttcgctgttt ctccggctac gttgggaaag
     7501 gagcgtgaag tgcagatcaa taatctggtt agcttattgg atggctattt tactccggat
     7561 ggaggtcagc atctgaatgt taacgtattt gataaggatt tgttgatcga cgctatggaa
     7621 catcctgaga aatatccgca acttacgatc cgtgtatccg gatatgcggt aaacttcgtg
     7681 aaactgactc gtgaacaaca gttggatgtt atttcaagaa ccattaacca ttcactgtaa
     7741 gtgataaaag gtaagataca ttcattagag agtttcggga ccgtagatgg tcccggaatt
     7801 cgctttgtcg tttttatgca gggctgcccg ttgcgttgcc tgtattgcca taacccggat
     7861 acttggaacc cgaaagggaa ggtgaaatat cagatgactc ccggcgagct attgacggaa
     7921 gtcttacagt ataagagttt tatcgcccgt gggggggtga ctgttaccgg tggggaacct
     7981 ttattgcaac ctgagtttct gaaagagttt ttccgtttgt gccaagagca aggtcttcac
     8041 acagctttgg acacatctgg tttcgtatgt acctcaaaag cttgggaagt cttggattat
     8101 gcggacttag tg
//
