LOCUS       c00249_gnlPro..         4298 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|OHCKOHPG_249.
ACCESSION   c00249_gnlPro..
VERSION     c00249_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 12:22:42
            Original ID  :: gnl|Prokka|OHCKOHPG_249
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4298
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4298
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[723:3689]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      724..3689
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4298
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4298
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..647
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 89.8; E-value: 3e-28)"
                     /gene_kind="other"
                     /locus_tag="ctg249_1"
                     /sec_met_domain="Fn3-like (E-value: 2e-29, bitscore: 89.8,
                     seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 1.6e-19,
                     bitscore: 58.8, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="CRMNPSPSGRSMSCSRGCAYQITLGSEAGNAICNVLWGEYNPSAK
                     LPMTFPAHVGQIPIYYQNKSTGRPTALQQTYKASYIDASTEPAYPFGYGLSYTDFGYSD
                     LKLLPGKDAGVHTEVSVKVTNTGKCAGEEVVQLYVQDKVASVTRPIKELKGFRKIHLNA
                     GESKTVAFQITDDALGFYDNEMNYMVEPGDFVFMVGGSSDDVQQITYNLKE"
     CDS             complement(724..1449)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg249_2"
                     /sec_met_domain="Radical_SAM (E-value: 6e-32, bitscore:
                     99.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINIHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDTRLLGESLGKYQQIQRVEILPYHRLGVHKYEAMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(1451..1885)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg249_3"
                     /sec_met_domain="Gly_radical (E-value: 6.5e-49, bitscore:
                     153.1, seeds: 99, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLR
                     YRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTKGAHHLNVNVLNREMLEDAM
                     EHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
     CDS             complement(1959..3689)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg249_4"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1113.7, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKTFTNGLWNKEINVSDFVSKNIAPYTGDASFLQGPTERTKR
                     IWDICLKALEEERNNNGVRSLDNTTVSTITSHKAGYIDKDNELIVGLQTDELLKRSIKP
                     FGGINVVAKACRENGVEVDDKVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRLALYGIDRLVEAKQEDLRNLTGPMTEARIRLREEVAEQIKALKDIK
                     VMGEYYGLDLSRPATSAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIFIEYDLAH
                     GKIDETFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTEAIGGRFNDGRVKVTKT
                     SFRFLQTLYNLGPSPEPNMTVLWSPELPEGFKDFCAKVSVDTSSIQYENDNLMREVRNC
                     DDYGIACCVSYQAIGKQIQFFGARANLAKALLLAINGGRCENTGTVMVKGIPVLTGNTL
                     KFEEVMANYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMAFIDTDPRINLAYGVAGL
                     SIAIDSLSAIKYAKVTARRNDIGLTEGFDMEGSFPCFGNNDDRVDHLGVDLVXXXILLQ
                     "
ORIGIN
        1 gctgtcggat gaatccgtca ccaagtgggc gttcaatgtc atgttcgaga gggtgtgctt
       61 atcaaattac cttggggagt gaggccggca acgccatatg caatgtgttg tggggagagt
      121 ataatccttc ggctaaattg cccatgacat ttcctgcaca cgtagggcag attcccattt
      181 attaccagaa taagagtacc ggtcgtccta ccgcattgca acagacttat aaggcatctt
      241 atatcgatgc ttctactgag cctgcctatc ctttcggata tgggctttcg tatacggatt
      301 ttggatattc tgatttgaaa ctgctaccgg gcaaggatgc aggcgtccat acggaggtgt
      361 cggtaaaagt gactaatacc ggtaagtgtg caggagagga agtagtccag ttgtatgtgc
      421 aggataaggt agcgtctgtt acgcgcccga taaaggagtt gaagggcttc aggaaaattc
      481 atctgaatgc gggtgaaagc aaaactgtag cttttcagat aactgacgat gcactgggat
      541 tctatgacaa tgagatgaat tatatggtag aacctggtga tttcgtcttt atggtgggtg
      601 gcagtagtga tgatgtacag cagattacct ataacttgaa agaataggat tnnnnnnnnn
      661 ntgtcctata aatgaaaagc aaggctgtct ggaatcatat ccggacagcc ttgcttttct
      721 gttttagttg gttatcacat tcgtgaaata ctctttgaac agtttttctg cccgttccag
      781 ttgttccgga gtattttcct tcacttcttt caatttgtaa tcccatccca tggcttcgta
      841 tttgtgaaca cccagacggt gataaggaag tatttccacc cgttgtatct gctggtattt
      901 gccaagactt tcacccagca ggcgggtgtc ttcttccgaa tcgctgtaac cggggaccag
      961 tacatagcgc agccagaagg ggtgttcatg ttcttcgagc caggcagcgg tgcggagtgt
     1021 ctgttcgttg cttcgtcccg tcagcgtacg gtggcgttcg ggattgaatt gcttgatgtc
     1081 cagcagcacg aggtcagtca ggctaaagag ctcttccacc ttttcattcc agatgcctcc
     1141 gttactgtcc agacagatat ggatgccgtt cgccttcagc tccttgaaaa gaggaatcag
     1201 cgcttctgct tgcagggtag gctcaccgcc ggagaaagta atacctcctt tcttcccgaa
     1261 gaatgccttt tggctgatag ccatttgcag aatctcttcc ggagtggtgg gagtgcctcc
     1321 ttttacatca atcgtatccg gattggcaca atacagacaa cggaacggac atccttgcag
     1381 gaaaacgacg agccggagac ccggcccgtc gaatgttccc atactttcgt aggagtgtat
     1441 gtttatcata ttacatcctt tcgtggaatg aacggctgat gacttccaac tgatgctcgc
     1501 ggctcaactt cacgaagttc actgcatagc cggaaacgcg gatggtgagt tgcggatact
     1561 tttcgggatg ttccatggca tcttccagca tttcgcggtt cagtacgttt acgttcaagt
     1621 ggtgggcacc tttggtgaag tagccgtcca tcatggttac caggttctct acacgttctt
     1681 ccggtgtcgg gcccagtgat ttggggacga tggagaacgt attgctgata ccatcctgcg
     1741 agtcgcggta acgcaatttg gctacagagc tcaaggaggc gatggcaccg ttcttgtcac
     1801 gtccgtgcat cggattggca cccggagcaa aggcaacacc tttggcgcgt ccgtccggag
     1861 tagcgcctgt ctttttgcca tacatcacgt tggatgtgat ggtcagcaat gaaagagtgg
     1921 ggcgggcatt tttgtagacg ggcagtttct tcagttcttc actgaagtag tatnnnnnnn
     1981 nnnactaaat ccacaccgag gtggtctaca cggtcatcgt tattaccaaa gcaagggaaa
     2041 gaaccttcca tatcaaaacc ctctgtcaga ccaatgtcat tacggcgggc agtgactttg
     2101 gcatatttga tggcagagag ggaatcgatg gcgatggaaa gtcctgccac accgtaagcc
     2161 agattgatgc gcgggtcggt atcgatgaat gccatctgag ccttttcgta ataatactta
     2221 tcgtgcatgt agtggataat gttcatggct tcgttgtata cgcgggcaat ttcagtcagt
     2281 accttcttgt agttggccat tacttcctcg aatttcagcg tgttgccggt cagtacggga
     2341 atacctttca ccatgacggt tccggtattt tcgcaacgtc cgccgttgat ggcgagcagc
     2401 aatgccttgg ctaggttggc acgtgcaccg aagaactgga tttgctttcc gatggcttgg
     2461 taagatacgc agcaggcaat accgtaatcg tcacagttgc gtacttcgcg catcaggtta
     2521 tcgttctcgt attggataga gcttgtgtct acggaaactt tggcacagaa atccttgaaa
     2581 ccttccggca gttccgggct ccacagtacc gtcatgtttg gttcgggaga aggaccgaga
     2641 ttgtataggg tttgcagaaa acggaaagag gttttggtca ctttcacgcg tccgtcgttg
     2701 aaacggccgc caatggcttc ggtcacccaa gtcgggtctc cggcaaagat gtcattgtac
     2761 gactgcatac gcagatggcg taccatgcgc agcttgatga cgaactggtc tatcagttct
     2821 tgtgcaaaag tttcatcaat cttgccgtga gccaaatcat actcgatgaa gatgtcgagg
     2881 aatgaagata cattgcccaa tgacatggca gcaccgtctt gttctttcac ggcagccaga
     2941 taagccatat atacccattg cacagcttct tgcgctgatg ttgcggggcg gctgaggtcg
     3001 agtccgtagt attcgcccat cactttgatg tctttcagag ccttgatttg ctctgctact
     3061 tcttcacgca gacggatacg ggcttcggtc atgggacctg tcagattgcg caagtcttct
     3121 tgcttggctt cgacaagtcg gtcgatgccg tagagtgcca gacgacggta gtcaccgatg
     3181 atacgtccgc gagcatagtt gtcgggaaga ccggtcagga atcccaatga gcggaaggaa
     3241 cggatttctt cggtatatac atcgaatacg ccgtcgttat gtgttttgcg gtaatgggtg
     3301 aagatgtctt ttactttatc gtccacttcc actccgtttt cacggcatgc ttttgctacc
     3361 acgttgatgc ctccgaaagg tttgatggat cgtttcaaca gttcatcagt ctgcaaaccg
     3421 acaataagtt cattgtcctt atcaatatac ccggctttat gagaagtgat ggtagatacg
     3481 gtggtattgt ccagtgaacg gacgccattg ttgttccttt cttcttcgag agccttcaga
     3541 cagatgtccc agatacgttt ggtacgttcg gtaggtccct gcaagaaaga tgcatccccg
     3601 gtatagggtg cgatgttttt actgacgaaa tcactgacat tgatttcttt attccaaagc
     3661 ccattggtaa aggttttgtt caattccata aaatttgttt ttgttagtag gttagataat
     3721 gttatccttt agagcgcaca aaaatactga ataaattccg taattttata agagatactt
     3781 ctctttttat gttgaacagc ataaaataac tattattagg tagtgaatgt ccgggggagt
     3841 agagatgtta atactttctt tatatgtggt ttatgaattg aaacaagaac cgaataataa
     3901 tctgcattat ttacaaatga tgtaggttta aggaacagtc gtattctgaa cctaattcag
     3961 tcgtatagtg aatgccattc agtcgtatac tgaatagggt tcaatcgtat actgaatgtg
     4021 gttctttgta aatatactga tagccagtaa gttataaaac tctgtcttgc ccttgataaa
     4081 tttcaacaaa tagcctaaat gccgttatgt tcgttccgta gtgggtataa acaagaattt
     4141 tggtataatt gctctgtttt tgtgcagaaa agtttgtaga ataaaaaatg atttgtatct
     4201 ttgcaccgct ttaaaagaaa agcacttctg aaaaagaagt tttggagagg tggcagagtg
     4261 gtcgattgcg gcggtcttga aaaccgttgt gctgcgag
//
