LOCUS       c00249_gnlPro..         3237 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|HNNPFEBK_249.
ACCESSION   c00249_gnlPro..
VERSION     c00249_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 11:39:51
            Original ID  :: gnl|Prokka|HNNPFEBK_249
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3237
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3237
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:1398]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..1398
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3237
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3237
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..671
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg249_1"
                     /sec_met_domain="Gly_radical (E-value: 1.7e-48, bitscore:
                     151.4, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 5e-40, bitscore:
                     125.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="IDSLSAIKYAKVTARRNDIGLTEGFDMEGSFPCFGNNDDRVDHLG
                     VDLVYYFSEELKKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPM
                     HGRDKNGAIASLSSVAKLRYRDSQDGISNTFSIVPKSLGPTPEERVENLVTMMDGYFTK
                     GAHHLNVNVLNREMLEDAMEHPEKYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM
                     "
     CDS             673..1398
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.5;
                     E-value: 1.7e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg249_2"
                     /sec_met_domain="Radical_SAM (E-value: 4.5e-32, bitscore:
                     99.5, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MINIHSYESMGTFDGPGLRLVVFLQGCPFRCLYCANPDTIDVKGG
                     TPTTPEEILQMAISQKAFFGKKGGITFSGGEPTLQAEALIPLFKELKANGIHICLDSNG
                     GIWNEKVEELFSLTDLVLLDIKQFNPERHRTLTGRSNEQTLRTAAWLEEHEHPFWLRYV
                     LVPGYSDSEEDTRLLGESLGKYQQIQRVEILPYHRLGVHKYEAMGWDYKLKEVKENTPE
                     QLERAEKLFKEYFTNVITN"
     CDS             complement(1467..3236)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fn3-like"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Glyco_hydro_3_C"
                     /gene_functions="other (smcogs) SMCOG1041:Fibronectin type
                     III-like domain (Score: 87.5; E-value: 1.7e-27)"
                     /gene_kind="other"
                     /locus_tag="ctg249_3"
                     /sec_met_domain="Fn3-like (E-value: 8e-29, bitscore: 87.5,
                     seeds: 729, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3 (E-value: 1.3e-57, bitscore:
                     183.2, seeds: 11, tool: rule-based-clusters)"
                     /sec_met_domain="Glyco_hydro_3_C (E-value: 1.4e-53,
                     bitscore: 169.6, seeds: 116, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="GLNWVFAPMVDISWDARWGRVMEGAGEDPYYGSRVAEARVRGLQG
                     DDLADTTTVMACMKHFAGYGAPIAGKEYNSVDMSMGHFANFYMPPYKAAIKVGAATVMS
                     AFNDFNNIPCTANDFLLNRLLREQWGFKGFVVSDYNSVEELVNHRYAVDKKDAAAKALN
                     AGLDMEMVSTCYLTYLKELVQEGKVKESVLDDAVRRILEKKYELGLFEDPFRYCNPERA
                     ARILNAPEVRNASLRMAERSVVLLENRESVLPLTAQTRKIALIGGLSKSQYDMAGAWAA
                     TTDRNKVVTLYDALVRKGLEVSYAEGYDMKSNQLTGLQEALQAAEASDVIVVAVGERFG
                     QSGEKASKVNIAIPEGQQKLVSELAKTGKPVIALVMCGRPVIFNEIREHADAVLCTWWL
                     GSEAGNAICNVLWGEYNPSAKLPMTFPAHVGQIPIYYQNKSTGRPTALQQTYKASYIDA
                     STEPAYPFGYGLSYTDFGYSDLKLLPGKDAGVHTEVSVKVTNTGKCAGEEVVQLYVQDK
                     VASVTRPIKELKGFRKIHLNAGESKTVAFQITDDALGFYDNEMNYMVEPGDFVFMVGGS
                     SDDVQQITYNLKE"
ORIGIN
        1 ccatcgattc cctctctgcc atcaaatatg ccaaagtcac tgcccgccgt aatgacattg
       61 gtctgacaga gggttttgat atggaaggtt ctttcccttg ctttggtaat aacgatgacc
      121 gtgtagacca cctcggtgtg gatttagtat actacttcag tgaagaactg aagaaactgc
      181 ccgtctacaa aaatgcccgc cccactcttt cattgctgac catcacatcc aacgtgatgt
      241 atggcaaaaa gacaggcgct actccggacg gacgcgccaa aggtgttgcc tttgctccgg
      301 gtgccaatcc gatgcacgga cgtgacaaga acggtgccat cgcctccttg agctctgtag
      361 ccaaattgcg ttaccgcgac tcgcaggatg gtatcagcaa tacgttctcc atcgtcccca
      421 aatcactggg cccgacaccg gaagaacgtg tagagaacct ggtaaccatg atggacggct
      481 acttcaccaa aggtgcccac cacttgaacg taaacgtact gaaccgcgaa atgctggaag
      541 atgccatgga acatcccgaa aagtatccgc aactcaccat ccgcgtttcc ggctatgcag
      601 tgaacttcgt gaagttgagc cgcgagcatc agttggaagt catcagccgt tcattccacg
      661 aaaggatgta atatgataaa catacactcc tacgaaagta tgggaacatt cgacgggccg
      721 ggtctccggc tcgtcgtttt cctgcaagga tgtccgttcc gttgtctgta ttgtgccaat
      781 ccggatacga ttgatgtaaa aggaggcact cccaccactc cggaagagat tctgcaaatg
      841 gctatcagcc aaaaggcatt cttcgggaag aaaggaggta ttactttctc cggcggtgag
      901 cctaccctgc aagcagaagc gctgattcct cttttcaagg agctgaaggc gaacggcatc
      961 catatctgtc tggacagtaa cggaggcatc tggaatgaaa aggtggaaga gctctttagc
     1021 ctgactgacc tcgtgctgct ggacatcaag caattcaatc ccgaacgcca ccgtacgctg
     1081 acgggacgaa gcaacgaaca gacactccgc accgctgcct ggctcgaaga acatgaacac
     1141 cccttctggc tgcgctatgt actggtcccc ggttacagcg attcggaaga agacacccgc
     1201 ctgctgggtg aaagtcttgg caaataccag cagatacaac gggtggaaat acttccttat
     1261 caccgtctgg gtgttcacaa atacgaagcc atgggatggg attacaaatt gaaagaagtg
     1321 aaggaaaata ctccggaaca actggaacgg gcagaaaaac tgttcaaaga gtatttcacg
     1381 aatgtgataa ccaactaaaa cagaaaagca aggctgtccg gatatgattc cagacagcct
     1441 tgcttttcat ttataggaca agaatcctat tctttcaagt tataggtaat ctgctgtaca
     1501 tcatcactac tgccacccac cataaagacg aaatcaccag gttctaccat ataattcatc
     1561 tcattgtcat agaatcccag tgcatcgtca gttatctgaa aagctacagt tttgctttca
     1621 cccgcattca gatgaatttt cctgaagccc ttcaactcct ttatcgggcg cgtaacagac
     1681 gctaccttat cctgcacata caactggact acttcctctc ctgcacactt accggtatta
     1741 gtcactttta ccgacacctc cgtatggacg cctgcatcct tgcccggtag cagtttcaaa
     1801 tcagaatatc caaaatccgt atacgaaagc ccatatccga aaggataggc aggctcagta
     1861 gaagcatcga tataagatgc cttataagtc tgttgcaatg cggtaggacg accggtactc
     1921 ttattctggt aataaatggg aatctgccct acgtgtgcag gaaatgtcat gggcaattta
     1981 gccgaaggat tatactctcc ccacaacaca ttgcatatgg cgttgccggc ctcactcccc
     2041 aaccaccaag tacacagtac cgcatcagca tgttccctta tctcattgaa gattaccgga
     2101 cgcccacaca tgacaagagc aatgacaggt tttccagtct ttgccagttc cgacaccagc
     2161 ttctgctgcc cctcgggtat ggctatattc actttggaag ccttctcgcc actctgaccg
     2221 aatctctcgc ctaccgccac aacaatgaca tccgaagcct cagcagcctg taatgcttcc
     2281 tgcaatccgg tcaactgatt gctcttcata tcatagcctt cagcatagct cacctccagt
     2341 cctttccgga ccaatgcatc atataaggtc accactttgt tccggtccgt agtggcagcc
     2401 caagcacctg ccatatcgta ctgggatttt gagagtcctc ctatcaaggc tatcttcctc
     2461 gtctgtgccg tcaacggaag cacagattcc ctattctcca gcaatacaac ggaacgctcg
     2521 gccatacgga gcgaagcatt acggacttcg ggcgcattga gtattctggc cgctctttcc
     2581 ggattacagt agcgaaaagg gtcctcaaac aatcccaact catatttctt ctccaatata
     2641 cgccgtactg catcatcaag tacactctcc tttactttcc cttcttgaac aagctccttc
     2701 agataagtca agtagcaggt acttaccatc tccatgtcca gaccagcatt cagtgctttg
     2761 gctgcggcat ctttcttgtc aacagcataa cggtggttca ctaattcctc taccgaatta
     2821 taatcggaca caacaaatcc cttaaatccc cactgctctc tcaatagccg gttgagcaag
     2881 aaatcattgg cagtacaagg aatattatta aaatcattga aagcactcat cacggtagct
     2941 gctcctacct ttatggccgc cttataggga ggcatataaa aattggcaaa gtggcccatt
     3001 gacatatcca cactattata ctcctttcct gctatcggtg caccatatcc ggcaaaatgt
     3061 ttcatacatg ccatgacagt cgtcgtgtct gccaaatcgt ctccttgcaa gcctctgaca
     3121 cgcgcctccg ctacccgaga accataataa gggtcctcac cagccccttc catgacgcgt
     3181 ccccaacgtg cgtcccacga aatatccacc atcggggcaa aaacccagtt caaacca
//
