LOCUS       c00082_gnlPro..         6118 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KPIIPBHD_82.
ACCESSION   c00082_gnlPro..
VERSION     c00082_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 19:20:52
            Original ID  :: gnl|Prokka|KPIIPBHD_82
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6118
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..6118
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:5690]"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..5690
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /neighbourhood="10000"
                     /product="TPP_AA_metabolism"
                     /protocluster_number="1"
     cand_cluster    1..6118
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(TPP_enzyme_C and not (PFL-like or
                     PFL_6_pcresol or PFL_9_tma or Gly_radical or Gly_430_pcre
                     or Gly_8-9_TMA or PFL_acetate1 or PFL_acetate2 or
                     Gly_radical_scatole or PFL_proline or PFL_toluene) and
                     minimum(2, [ALS_ss_C, AlaDh_PNT_C, Arginase, BKACE,
                     BcrAD_BadFG, Beta_elim_lyase, CPSase_L_D2, Cys_Met_Meta_PP,
                     ELFV_dehydrog, GRDB, Glu_syn_central, Glu_synthase,
                     Gly_reductase, HAAA1, HG2B1, HG2B2, HGD-D, HleuD1, HleuD2,
                     HleuS1, HleuS2, Homoserine_dh, ILVD_EDD, IlvC,
                     Lactoyl-CoA1, Lactoyl-CoA2, Lyase_aromatic, MAAL_C,
                     OKR_DC_1_5_lysine, OKR_DC_1_5_ornithine,
                     OKR_DC_1_7_arginine, OKR_DC_1_C, OTCace, PALP,
                     Proline_reductase, Sacchrp_dh_C, TPL, Thr_dehydrat_C, TnaA,
                     Urocanase]) and not (POR or POR_N or PFOR_II))"
                     /kind="single"
                     /product="TPP_AA_metabolism"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6118
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="TPP_AA_metabolism"
                     /region_number="1"
                     /rules="(TPP_enzyme_C and not (PFL-like or PFL_6_pcresol or
                     PFL_9_tma or Gly_radical or Gly_430_pcre or Gly_8-9_TMA or
                     PFL_acetate1 or PFL_acetate2 or Gly_radical_scatole or
                     PFL_proline or PFL_toluene) and minimum(2, [ALS_ss_C,
                     AlaDh_PNT_C, Arginase, BKACE, BcrAD_BadFG, Beta_elim_lyase,
                     CPSase_L_D2, Cys_Met_Meta_PP, ELFV_dehydrog, GRDB,
                     Glu_syn_central, Glu_synthase, Gly_reductase, HAAA1, HG2B1,
                     HG2B2, HGD-D, HleuD1, HleuD2, HleuS1, HleuS2,
                     Homoserine_dh, ILVD_EDD, IlvC, Lactoyl-CoA1, Lactoyl-CoA2,
                     Lyase_aromatic, MAAL_C, OKR_DC_1_5_lysine,
                     OKR_DC_1_5_ornithine, OKR_DC_1_7_arginine, OKR_DC_1_C,
                     OTCace, PALP, Proline_reductase, Sacchrp_dh_C, TPL,
                     Thr_dehydrat_C, TnaA, Urocanase]) and not (POR or POR_N or
                     PFOR_II))"
                     /tool="gutsmash"
     CDS             2..1552
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ILVD_EDD"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg82_1"
                     /sec_met_domain="ILVD_EDD (E-value: 1.4e-196, bitscore:
                     642.4, seeds: 918, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="AMGHDGMLYSLPSRDIIADSVEYMVNAHKADAMVCISNCDKITPG
                     MLMAAMRLNIPTVFVSGGPMEAGEWNGQHLDLIDAMIKSADDSVSDQEVANIEQNACPT
                     CGCCSGMFTANSMNCLNEAIGLALPGNGTIVATHENRTRLFEDAAKLIVENAMKYYEEG
                     DESVLPRSIATRQAFLNAMTLDIAMGGSTNTVLHLLAVAHEAGVDFKMDDIDMLSRKTP
                     CLCKVAPNTQKYHIQDVNRAGGIIAILAELAKGGLIDTSVLRVDGMSLAEAIDQYSITS
                     PNVTEKAMSKYSSAAGNRFNLVLGSQGAYYQELDKDRANGCIRDLEHAYSKDGGLAVLK
                     GNIAQDGCVVKTAGVDESIWKFTGPAKVFDSQEAACEGILGGRVVSGDVVVITHEGPKG
                     GPGMQEMLYPTSYIKSRHLGKECALITDGRFSGGTSGLSIGHVSPEAAAGGNIGKIVDG
                     DIIEIDIPARTINVRLTDEELAARPMTPVTRDRYVPKSLKAYASMVSSADKGAVRLID"
     CDS             1600..3297
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: TPP_enzyme_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg82_2"
                     /sec_met_domain="TPP_enzyme_C (E-value: 1.6e-47, bitscore:
                     149.6, seeds: 323, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSKDLITGAEAMMRSLEHQGVTTIFGYPGGSIMPTFDALYDHQNT
                     LNHILVRHEQGAAHAAQGYARVSGKVGVCLVTSGPGATNTITGIADAMIDSTPIVVIAG
                     QVGTGFLGTDAFQEVDLVGITQPIAKWSYQIRRAEDVAWAIARAFYIASSGRPGPVVLD
                     FAKNAQVEKTKYEPTQQEFIRSYVPVPDTDEESVKAAAELINNAERPLVLVGQGVELGS
                     AQEELRIFIEKADMPAGCTLLGLSALPTDHPLNKGMLGMHGNLGPNINTNKCDVLIAVG
                     MRFDDRVTGNLATYAKQAKVIHFDIDPAEVNKNVKVDIAVLGDCKKTLAAVTGLLKKNR
                     HTEWVDSFKEYEAVEEEKVIRPELHPATDSLSMGEVVRAVSEATRHEAILVTDVGQNQM
                     ISARYFKYTRERSIVTSGGLGTMGFGLPAAIGATFGRPDRTVCVFMGDGGLQMNIQELG
                     TIMEQKAPVKIICLNNNYLGNVRQWQAMFFNRRYSFTPMLNPDYMKIASAYDIPSKRVF
                     SREELKAAIDEMLSTDGAFLLEACVVEEGNVLPMTPPGGSVNQMLLEC"
     CDS             3316..3879
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: ALS_ss_C"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg82_3"
                     /sec_met_domain="ALS_ss_C (E-value: 2.7e-20, bitscore:
                     60.9, seeds: 685, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSDKTLYTIIVHSENIAGLLNQVTAVFTRRQINIESLNVSASSIK
                     GVHKYTITAWTDKDTIEKVVKQIEKKIDVIQAHYFTEDEIYFHEIALYKVSTPAFQETP
                     EASKVIRRYNARIVEVNPVFSIVEKNGMSEEITSLYEELRALKCVLQFVRSGRVAITTS
                     CFERVNEFLDGQEAKYKQSKKEQE"
     CDS             3879..4622
                     /locus_tag="ctg82_4"
                     /transl_table=11
                     /translation="MSEENKIGTYQFVAEPFHVDFNGRLTMGVLGNHLLNCAGFHASDR
                     GFGIATLNEDNYTWVLSRLAIELDEMPYQYEKFSVQTWVENVYRLFTDRNFAVIDKDGK
                     KIGYARSVWAMINLNTRKPADLLALHGGSIVDYICDEPCPIEKPSRIKVTSNQPVATLT
                     AKYSDIDINGHVNSIRYIEHILDLFPIELYQTKRIRRFEMAYVAESYFGNELSFFCDEV
                     SENEFHVEVKKNGSEVVCRSKVIFE"
     CDS             4686..5690
                     /gene_functions="biosynthetic (rule-based-clusters)
                     TPP_AA_metabolism: IlvC"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) IlvN"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg82_5"
                     /sec_met_domain="IlvN (E-value: 1.5e-47, bitscore: 149.7,
                     seeds: 43, tool: rule-based-clusters)"
                     /sec_met_domain="IlvC (E-value: 4.6e-36, bitscore: 112.7,
                     seeds: 295, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAQLNFGGTVENVVIRDEFPLEKAREVLKNETIAVIGYGVQGPGQ
                     ALNLRDNGFNVIVGQRQGKTYDKAVADGWVPGETLFGIEEACEKGTIIMCLLSDAAVMS
                     VWPTIKPYLTAGKALYFSHGFAITWSDRTGVVPPADIDVIMVAPKGSGTSLRTMFLEGR
                     GLNSSYAIYQDATGNAMDRTIALGIGIGSGYLFETTFIREATSDLTGERGSLMGAIHGL
                     LLGQYEVLRENGHTPSEAFNETVEELTQSLMPLFAKNGMDWMYANCSTTAQRGALDWMG
                     PFHDAIKPVVEKLYHSVKTGNEAQISIDSNSKPDYREKLEEELKALRESXXXX"
ORIGIN
        1 cgctatggga catgacggaa tgctttactc gcttccttcc cgtgatatca tagcggacag
       61 cgtagaatat atggtcaatg cgcacaaggc ggatgcaatg gtatgtatca gcaactgcga
      121 taagatcacc ccgggaatgt tgatggctgc catgcgcctg aatatcccta ccgtattcgt
      181 atccggcgga ccgatggaag ccggagagtg gaacggccag catctggact tgatcgatgc
      241 catgattaaa tcggcggacg acagtgtaag cgatcaggaa gtagcgaata tcgaacagaa
      301 tgcctgtcct acctgcggat gttgttctgg aatgtttacg gcaaactcga tgaactgtct
      361 gaatgaagct atcggcttgg ctcttccggg aaatggcaca atcgtggcta cgcacgaaaa
      421 tcgtacgaga ctgtttgaag atgctgccaa actgattgtg gaaaatgcaa tgaaatatta
      481 tgaagaaggt gacgaaagtg tacttccccg cagcatagct acccgtcagg cattcctcaa
      541 tgccatgacg ctggatattg cgatgggcgg ttctaccaat acggtgcttc acctgttggc
      601 cgtggcacat gaggcaggag tcgatttcaa gatggacgac atcgatatgc tgtcacgcaa
      661 gacaccctgc ctgtgtaaag tcgctccgaa tactcaaaaa tatcatattc aggacgttaa
      721 ccgtgcgggt ggcatcattg ctatcctggc agagttggca aaaggcggtc tgattgatac
      781 ttccgttctc cgtgtggatg gcatgtcgct ggcggaagcg atcgatcaat attcaattac
      841 cagcccgaac gttacggaaa aagcaatgag caaatattca agtgcggcag gaaacagatt
      901 caatctcgta cttggttctc agggagctta ttatcaggaa ttagataaag atcgcgcgaa
      961 cggatgtatc cgtgatctgg agcacgccta tagcaaggac ggaggcctgg cggtactgaa
     1021 aggaaatatc gctcaggacg gttgcgtagt aaaaacggcg ggtgtagacg aaagcatctg
     1081 gaagtttacc ggacctgcca aagtattcga ctcacaagaa gcagcctgtg aaggcatcct
     1141 cggaggccga gtcgtcagcg gcgacgttgt cgtcatcacg cacgaaggtc cgaagggcgg
     1201 tcccggtatg caggaaatgc tttacccgac ttcttatatc aaatcccgtc acctcggcaa
     1261 agaatgtgcc ttgattaccg acggacgttt cagtggtgga acttccggat tgagtatcgg
     1321 acacgtttct cccgaagccg ctgccggagg taatatagga aaaatagtag atggagatat
     1381 catcgaaatc gacattcccg cccgaacgat taacgtgcga ctaacagatg aagaactggc
     1441 tgcccgcccg atgactcccg tcacccgtga ccgttacgtg ccgaagagcc tgaaagcata
     1501 cgccagcatg gtaagctctg ccgataaggg agcagtgaga ttaatagact gatccacttc
     1561 attatccatt atgaatcatc cattatccat taaagaacta tgagtaagga cttaataaca
     1621 ggcgcagagg caatgatgcg ctctctggaa catcagggag tgactaccat tttcggttat
     1681 cccggcggtt ccatcatgcc tacgttcgat gccctgtacg accatcaaaa tacattgaac
     1741 catatcttgg ttcgccatga acagggagct gcccatgcgg cacaaggcta cgcccgtgta
     1801 tcgggtaaag tcggtgtttg cttggtgaca agcggccccg gagcaaccaa tacgatcaca
     1861 ggtattgcag atgcgatgat agacagtacg cctatcgttg tcattgcagg acaggtggga
     1921 acaggctttc ttggaacaga cgcttttcag gaagtcgatc tcgtaggtat cactcagcca
     1981 atcgctaaat ggagctatca gattcgccgt gccgaagacg tagcctgggc gatagcacgt
     2041 gctttctaca ttgccagtag tggtcgtccc ggtccggtgg tactcgactt tgccaagaat
     2101 gcgcaggttg aaaaaacaaa atacgagccg acgcagcagg agtttatccg cagctatgtt
     2161 ccggttcccg atacagacga agaatcagta aaggctgccg ccgaactgat caataacgcc
     2221 gaacgcccgc ttgtcttggt aggacaggga gtggaactgg gaagcgctca ggaagaactg
     2281 cgtatcttta tcgaaaaggc ggatatgccc gcaggttgta ccttgctcgg actttccgca
     2341 ttgcctaccg accatccgtt gaataaagga atgctgggta tgcacggaaa tctgggaccg
     2401 aatatcaata ctaacaaatg tgatgtactg attgctgtcg gtatgcgttt cgacgaccgt
     2461 gtgaccggta atctggctac ttacgccaaa caagcgaaag tgatccattt tgacattgat
     2521 ccggcggaag taaataagaa tgtaaaggta gacattgccg ttttgggcga ctgcaaaaag
     2581 acgctggctg ctgtgaccgg actgctgaaa aagaaccggc acaccgagtg ggtcgatagt
     2641 ttcaaggaat atgaagcggt agaggaagaa aaagtcattc gtcccgaact tcatcctgca
     2701 acggattcac tgagtatggg cgaagtggtg cgtgcggtaa gcgaagcgac ccgtcacgaa
     2761 gccatattgg tgacagatgt aggtcagaat cagatgatct ctgcccgcta ctttaaatat
     2821 accagggaac gtagtatcgt tacttcgggt ggacttggca cgatgggttt tggccttccg
     2881 gcagctatcg gagctacttt cggccgtccc gaccgcactg tatgtgtatt tatgggtgac
     2941 ggcggactgc aaatgaacat tcaggaactg ggtacgatca tggaacagaa agctccggtg
     3001 aagattatct gcctgaacaa caattatctc ggcaacgtgc gccaatggca ggctatgttc
     3061 tttaatcgcc gttattcatt tactccgatg ctgaatccgg attacatgaa aatcgcttcc
     3121 gcctacgata ttccttccaa acgtgtcttt tcacgggaag aattgaaagc ggccatcgac
     3181 gaaatgctct cgacagacgg ggcgttcctg ctcgaagcct gtgtggtaga agaaggcaat
     3241 gtcctcccga tgacccctcc gggcggttca gtcaatcaga tgttgctgga atgttaatcc
     3301 ttaatttaga aagttatgag tgataagaca ttatatacga ttatagttca ttctgaaaat
     3361 atagccggat tgctgaatca ggtgaccgct gtatttaccc gtcggcaaat aaacatcgag
     3421 agtttgaacg tttctgcttc ttccattaaa ggagtacata aatatacgat tacagcgtgg
     3481 actgataagg acactatcga aaaggtggtg aagcagatcg aaaagaagat tgatgtgatt
     3541 caggcgcact attttacgga agacgagatt tatttccatg agatagcctt atataaggta
     3601 tctactcctg cttttcagga gactccggaa gcatctaaag tgatccgcag atataatgcc
     3661 cgcattgtag aggtgaatcc ggtattctct atcgtcgaga agaacggcat gagtgaagag
     3721 attacttccc tttacgaaga acttcgggct ttgaaatgtg tgctgcaatt tgtccgttcg
     3781 ggccgtgtgg ctattactac cagctgtttc gaacgtgtga atgagttcct ggacggacag
     3841 gaagcgaagt ataagcaaag taaaaaggaa caggagtaat gagtgaagaa aataaaatag
     3901 ggacttatca gtttgtggca gagccgtttc acgtagattt taacggccgc ctgaccatgg
     3961 gcgtgctggg gaatcatctg ctgaattgcg caggttttca tgccagcgac cgtggatttg
     4021 gtattgctac gctgaacgag gacaattaca catgggtgct ttcccgtctg gcgatcgagc
     4081 tggacgagat gccttaccaa tacgagaagt tctcggttca gacatgggtg gagaatgtct
     4141 atcgtctctt taccgaccgt aactttgccg tcattgacaa ggatggaaag aagatcggct
     4201 atgcccgttc cgtatgggcg atgatcaatc tgaatactcg taagcctgcc gatctgttgg
     4261 cattgcatgg aggaagcatc gtcgattata tatgcgatga accctgcccg atagaaaagc
     4321 cttcgcgtat caaggtgaca agcaatcagc ctgttgcgac gctgacggca aaatacagtg
     4381 atatcgatat taacggacac gtaaacagta ttcgttatat cgagcacata cttgacttgt
     4441 ttccgataga actgtatcag acgaaacgta tccgccggtt tgaaatggcg tatgtggctg
     4501 aaagttattt cggaaatgaa ctctctttct tctgtgatga ggtgagtgaa aacgagtttc
     4561 atgtagaggt gaagaaaaac ggcagcgagg tagtatgccg ttccaaagtg atatttgaat
     4621 aatatagagt ataaaatcaa ttaataacag atggcgcaag tcatcaaaac aaaaaaacaa
     4681 aaaaaatggc acagttgaat tttggcggaa ctgtagaaaa tgtagttatc cgtgatgaat
     4741 ttccattgga aaaagctcgt gaagtattga aaaatgaaac aatcgctgta atcggttatg
     4801 gcgtacaagg tcccggacag gctctgaacc ttcgtgataa cggtttcaat gtaatcgttg
     4861 gtcaacgcca gggaaagaca tatgacaaag cggttgctga cggatgggtt ccgggtgaaa
     4921 ctttgttcgg tattgaagaa gcttgcgaaa aaggtacgat cattatgtgc ctgttgtctg
     4981 atgcagcagt aatgtctgta tggcctacta tcaagcctta cctgactgca ggaaaagctc
     5041 tttatttctc tcatggtttt gctattacat ggagtgaccg cacaggtgta gttcctcctg
     5101 cagatatcga cgtaatcatg gttgctccta aaggttcggg tacatccttg cgtactatgt
     5161 tccttgaagg tcgcgggttg aactcttcat acgctatcta tcaggatgca acaggcaacg
     5221 ctatggacag aacaatcgca ttgggtatcg gtatcggttc aggttatttg ttcgaaacaa
     5281 ctttcatccg cgaagctact tccgacctga caggcgaacg tggttcattg atgggagcta
     5341 tccatggtct gttgctggga caatacgaag tgttacgtga aaacggtcac actccttccg
     5401 aagcattcaa cgaaactgta gaagagctga ctcagtcatt gatgccgttg tttgcaaaga
     5461 acggtatgga ctggatgtat gctaactgct ctactacagc tcaacgtggt gctctcgact
     5521 ggatgggccc cttccacgat gctatcaaac cggtcgttga aaagttgtat cacagtgtga
     5581 agactggtaa cgaagcacag atttcaatcg actctaactc caaaccggat tatcgtgaga
     5641 aactggaaga agaactgaaa gcattgcgcg aaagcgannn nnnnnnntaa aagatactga
     5701 taatataggt gaaaaagagc atttatgatt acctttacgg caacaaataa aacagaatag
     5761 gcaatgaaga aactttttac ttacagaaaa tcgatacgta tgaagatgat ttcttcttta
     5821 atgaatagta tatcagaatg ttacattaaa agatatttaa gttattctct accgaatggt
     5881 agctgtaata ccatttcaag tcctttttat atttcgtgtt ccattagtga ttcgttgata
     5941 tgatgcagaa ttttattctg tagcaggaaa aagtatgcgt taaattgaat ttaagacaga
     6001 gcttttggaa cagaatttag tgggaaaata acattgtttg ttttgtttgt aaatgggagt
     6061 cttctttcgt gagaaagggg gcttcttttt ttatcccgat gattcaggag gataccgc
//
