LOCUS       c01140_gnlPro..         1642 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AMGILMNL_1140.
ACCESSION   c01140_gnlPro..
VERSION     c01140_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 13:08:21
            Original ID  :: gnl|Prokka|AMGILMNL_1140
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 1642
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..1642
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[171:1642]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      172..1642
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..1642
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..1642
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..179
                     /locus_tag="ctg1140_1"
                     /transl_table=11
                     /translation="QKEALHRLVHELLQRYPEAKVVGHRDLSPDTNYNGIVDPWERIKE
                     CPCFEVIGEFSIN"
     CDS             complement(172..900)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 72.5;
                     E-value: 1.4e-22)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1140_2"
                     /sec_met_domain="Radical_SAM (E-value: 5.5e-30, bitscore:
                     92.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTPPEEIVRMAMSQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSN
                     GGLWNEDVEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRY
                     VLVPGYSDFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTP
                     EQLEKAAEVFKEYFTTVVVN"
     CDS             complement(914..1642)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg1140_3"
                     /sec_met_domain="Gly_radical (E-value: 2.4e-48, bitscore:
                     150.9, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 4.7e-53, bitscore:
                     168.0, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="LVDTNPRINLAYGVAGLSIALDSLSAIKYAKVTARRNDIGLTEGF
                     DIEGEFPCFGNDNDKVDHLGVDLVYFFSEELKKLPVYKNARPTLSLLTITSNVMYGKKT
                     GATPDGRAKGVAFAPGANPMHGRDKNGAIASLSSVAKLRYRDSQDGISNTFSIVPKSLG
                     ATDEDRIENLVTMMDGYFTKGAHHLNVNVLNRDMLYDAMEHPENYPQLTIRVSGYAVNF
                     VKLSREHQLEVISRSFHERM"
ORIGIN
        1 gtcagaagga ggcgctacat cgcttggtac acgaattgtt gcagcgttat ccggaagcta
       61 aagtagtagg gcatcgggat ttaagtcccg acaccaacta taatgggatc gtagatcctt
      121 gggaacggat taaagaatgc ccttgctttg aggtcattgg agaattctcc attaattaac
      181 taccaccgta gtgaaatact ctttgaatac ctccgcagct ttttcaagct gttcgggggt
      241 attttctttt acccctttca tcttgtattc ctgtcccatc gcttcgtact tatgtacgcc
      301 cagtgtatgg taaggcagta tttccacccg ttggatcatc ttgtattttc cgagtgcttc
      361 tcccagtgcg cgtatgtctt cttcaaaatc gctgtatccg ggcactagca cgtagcgtag
      421 ccagaaaggt ttctcctgtt cttccagcca ggcggccgtg cggatggttt gctcgttgct
      481 tcttccggtg agcgtttgat ggcggttagg gttgaattcc tttatatcca ataacacaag
      541 atcggtcagt ctgaataatt cttctacgtc ttcgttccaa agtcctccgt tgctgtccag
      601 acagacatgg atgccttttt ctttcagttc gcggaccagc gggacgagtg ctttcgcctg
      661 aaacgtcggt tcgcctccgg agaaagtgat tcctcctcgt ttcccgaaga aggggcgctg
      721 gctcattgcc atgcggacga tttcttccgg tggggtaggc gtgcctccct ttcctgctat
      781 cgtatccgga ttggcgcaat agagacaacg gaagttgcat ccttgaagaa aaacgacgag
      841 ccgtaagccc ggcccgtcga atgttcccat actttcgtat gaatgtacgt ttatcatcat
      901 atagagtaat agattacata cgttcgtgga agctacggct gataacttcc agttggtgtt
      961 cgcggctcag cttcacgaag tttacggcat agccggaaac gcggatagtc agctgcggat
     1021 agttttccgg atgctccatg gcatcgtaca gcatatcacg gttcagtacg tttacgttca
     1081 ggtggtgggc acctttggtg aagtaaccgt ccatcatcgt tacgaggttc tcgatacggt
     1141 cttcgtccgt agcacccagt gatttcggaa cgatagagaa ggtgttgctg ataccgtcct
     1201 gtgagtcacg gtaacgaagt ttcgctacag aactcagaga agcgatcgca ccattcttgt
     1261 cgcgtccgtg catcgggttg gcacccggag cgaaggcaac acctttggca cgtccgtcgg
     1321 gagtagcacc ggtctttttg ccgtacatca cattggaagt gatagtcagc aaggagaggg
     1381 taggacgggc attcttgtaa acaggcaatt tcttcaattc ttcgctgaag aagtatacca
     1441 ggtctacgcc aaggtggtct accttgtcgt tgtcgttacc gaagcaaggg aattctcctt
     1501 cgatgtcgaa gccttctgtc agaccgatat cattgcggcg ggcagttact ttcgcatatt
     1561 tgatggcaga cagtgaatcg agggcgatgg aaagtccggc tacaccgtaa gctaggttga
     1621 tgcgcgggtt cgtatctaca ag
//
