LOCUS       c00161_gnlPro..         3868 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|LCHPLFKF_161.
ACCESSION   c00161_gnlPro..
VERSION     c00161_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 19:37:59
            Original ID  :: gnl|Prokka|LCHPLFKF_161
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3868
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..234)
                     /locus_tag="ctg161_1"
                     /transl_table=11
                     /translation="MKSYACLCLLLAGVFCVACFSGACADELPPTLKPQVNPRAVYDWK
                     KRHEAVMERNRTVKPEYVVFGDSITHRWGGEPS"
     protocluster    1..3868
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[514:3578]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      515..3578
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3868
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3868
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             515..2782
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg161_2"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-46, bitscore:
                     145.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 5.5e-282, bitscore:
                     924.1, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISIVKDLKESTALPQEWQGFKPGTWTESIDVRDFIQHNYTPYSG
                     NEEFLSGPSQRTLRLWDELKVLLKREIDNGGVLDADEEVVSSITSHKPGYIDKELEVVV
                     GLQTDAPLKRALMPFGGLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDAYTSDI
                     RAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDKLIAERRKDLKNREHSPLTDELIRL
                     REEMSEQIRALEELAQLGASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLGR
                     VSTFFDIYFTRDLEQRLITEEEVQEIIDQFVMKLRIVRFIRTPDYNNLFSGDPTWVTES
                     IGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETSS
                     VQYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGK
                     QVAPPSPRYTEEILNYDEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHD
                     PEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVD
                     DIACSLVSNFMEKLRRLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGA
                     NPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAY
                     FAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTFH
                     TR"
     CDS             2799..3578
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 53.6;
                     E-value: 9.1e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg161_3"
                     /sec_met_domain="Radical_SAM (E-value: 2e-32, bitscore:
                     100.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELRPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRRGGFPDCVH"
ORIGIN
        1 ggaaggctcg ccgccccagc ggtgggtaat gctgtcacca aagacgacat attccggttt
       61 gacggtccgg ttccgctcca tcactgcttc atggcgtttt ttccagtcat acacagcccg
      121 cgggttcacc tggggcttca gcgtgggcgg caattcatca gcacaggcac cggagaaaca
      181 agcaacgcaa aaaactcccg ccagcaataa acaaaggcat gcataagatt tcataacggt
      241 aagcgttgag atactgcaat agagattagg aaagtaaaga gaaataatct tccgtcactt
      301 ttccgcaatc gcatgttccc ctcattttcc gctccgcaac ggacagacag gtgaaacatg
      361 ccaaagccag cgcacccgcg ttctcttcgc aaagaattag ccttgttgtc ccccggaatt
      421 cacgatataa ttacgcggcg cggagttttg ccgtttccag cctttcaggg gctgcggaac
      481 gtttttctcc agtcttcttc actacattct atttatgatc agcatagtaa aagacttgaa
      541 ggagtccacc gcccttccgc aggaatggca gggattcaag ccgggaacat ggacggagtc
      601 cattgacgta cgggatttca tccagcataa ctacacgccc tattccggca atgaagagtt
      661 tctttccggc ccctcccagc gcacgcttcg tttatgggac gagctgaaag tcctgctgaa
      721 acgggaaata gacaacggcg gcgtgctgga tgctgatgaa gaagtggtat cctccatcac
      781 atcccacaag cccggctaca ttgacaagga actggaagtg gtcgtaggcc tccagacgga
      841 cgcccccctg aaaagggccc tgatgccctt tggcggcttg cgcatggccc aacaggccct
      901 ggaatcctac gggttcaaga tgtgcgagaa aaccgcggac atttttaaaa agatacgcaa
      961 gacgcacaat gaaggcgttt tcgacgccta cacttccgat atccgggcgg cccgttccgc
     1021 cgggattatt accggccttc cggatgccta tggccgcggc cgcatcatcg gggactaccg
     1081 ccgggtggcc ctgtacggca cggataagct gattgccgaa cgccgcaagg acctcaaaaa
     1141 ccgtgaacat tcccccctta cggatgagct gatccgtctg cgcgaagaaa tgagcgaaca
     1201 aatacgcgcc ctagaagaac tcgcccagct gggcgcctcc tacggctgcg acctcactcg
     1261 ccccgccgca aacgcccgcg aagccgtgca atggacgtac ctgggctacc tggctgccgt
     1321 gaaggaacag aacggagcgg ccatgtccct gggccgcgtt tccactttct tcgacattta
     1381 tttcacccgc gacctggaac agaggctcat cacggaagag gaagttcagg aaatcatcga
     1441 ccagttcgtg atgaagctgc gcatcgtccg tttcatccgc acgcctgatt acaataacct
     1501 gttctccgga gaccccacct gggtgacgga atccatcggc ggcatgggag aggacgggcg
     1561 caccctcgtc acccgcagtt ccttccgcat gctgcagacc ctgtacaacc tggggccggc
     1621 tccggaaccg aacctgaccg ttctgtggtc ccggaacctg ccggaagcct tcaaaagctt
     1681 ctgcgccaag gtttccattg aaacatcctc cgtccaatac gagaacgatg acctgatgcg
     1741 cccacattgg ggcgacgact acggcatcgc atgctgcgtg tccgccatgc gcatcggcaa
     1801 gcagatgcag ttcttcggag cgcgcgccaa cctcgccaag tgccttctct acgccctgaa
     1861 cggcggcgtg gatgaactca agggcaagca ggtggccccg ccctcccccc gctacacgga
     1921 ggaaattctc aactatgatg aggtgatggc cctgtacgac aagatgcagg actggctcgc
     1981 caaaacttac attgacgcgc tgaacatcat ccactacatg cacgacaaat actgctatga
     2041 acgcattgaa atggcgctgc atgatccgga aattctgcgc acgatggcta cgggaatcgc
     2101 cgggctttcc gtggcggcgg actccctgtc cgccatcaag tatgccaccg taaaagccat
     2161 ccgcaatgaa gaagggctga ttgtggactt caagacggaa ggggaattcc cctgttacgg
     2221 gaacaatgac ccgcgcgtgg acgacatagc atgcagcctg gtcagcaatt tcatggaaaa
     2281 actgcgcagg ctccacactt accgcaattc cctgcccacc cagtccatcc tgacgatcac
     2341 ctccaacgtg gtgtacggca aaaagacggg caacacgccg gacggccgcc gagccgggga
     2401 accgttcgcc cccggagcca accccatgca cggaagggac aggaacggag ccgtggcctc
     2461 catgctctcc gtagccaagc tgtcctatga cgactccctg gacggcatct cctacacctt
     2521 ctccatcgtt cctcaggccc tgggcaagga ggaacgtgaa cgccgcgtca agctcgtctc
     2581 cctgctggac gcctactttg ccgctacagg tcaccacatt aatgtgaacg tactggaacg
     2641 ggaaacgctt ctcgacgcca tggatcaccc ggaaaaatac ccgcagctta ccatccgcgt
     2701 ttccggctat gccgtgaatt tcatcaagct gacccgggaa cagcagcagg aggtcatcaa
     2761 ccgcactttc cacacccgtt aacccggact gcaagcggat gccgcaggtt tttccacaaa
     2821 accatgatcc ggacggagaa agctccgtca ccggtttggt tcattccgtg gaatcctgcg
     2881 gcaccgtgga cggccccggc atccgcttcg tccttttcct gtccggctgc agcctgcgct
     2941 gccgctactg ccataatccg gatacctcct acgtgcggag gggccggctc aggagcgcag
     3001 atgatgtcct caaggaaata gcccgttacc gggatttttt gcaggcggcg ggcggcggcg
     3061 tcaccctctc cggcggggat cccctcttcc agccggattt tgccggtgcc gtgctgaaag
     3121 gctgccggaa actgggcctg cacacctgcc tggacacctc cggccatctg ggcgtgaatg
     3181 cgggcgaaga aatgctggcg gatacggacc tggtcctgct ggatatcaag gcatggaatc
     3241 cgaaacggta tcgcgccctg acaggcgggg aactgcgccc tacactggag tttgcggaac
     3301 gcctggcctc cctccgcaag cccgtgtggc tgaggtatgt gctggtacct ggcgttacgg
     3361 acaatctgga agaaattgag gaactctccc gccatgcggc gcttctgggc aacgtggaac
     3421 gcgtggacgt gctccctttc caccagctgg ggagattcaa atgggatgaa ttgaagctgg
     3481 actatacttt gaaggacgtc ccggaagcgt ccgcagaatt ggcggaacag gtccgcggca
     3541 ttttccggcg gggcggcttt ccggattgcg tgcattaacc ggattttcgc gctttggcgc
     3601 caccaaccgc agcaaaccgg acgcaggcat ccccctcttc cggatgaggg gccctcttcc
     3661 ctccactaca gaatccatgc aaaaaggaag acgcatttct ggaattgctc cggaatacgg
     3721 catttccgca attcctttcg ttattccatc attctttacg gcaacggcag cattcccgcc
     3781 gtttttccgc caacgccggg ggcggcattc cgtcccttcc cagattattc tttcccggaa
     3841 atttcccgca aggcttccag tacttccc
//
