LOCUS       c00253_gnlPro..         5890 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|AFBBEMON_253.
ACCESSION   c00253_gnlPro..
VERSION     c00253_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 14:13:07
            Original ID  :: gnl|Prokka|AFBBEMON_253
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5890
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5890
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[3523:5890]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3524..5890
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..5890
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5890
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             complement(163..747)
                     /locus_tag="ctg253_1"
                     /transl_table=11
                     /translation="MTTKLKDLFDTFLNIAASSQKLGINLIRVAILIIFVWIGGLKFYN
                     YEAEGIVPFVANSPFMSFFYAKSAPEYKEYKLKEGEFNEAKHQWHVENNTYIFSHGLGI
                     MIMAIGILTFLGIFSPKIGLIGSGLAIIMTLGTLSFLVTTPEVWVPDLGSGEHGFPLLT
                     GAGRLVIKDTAIIAGAIVVLSDCAQRILKKK"
     CDS             complement(925..2139)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Radical_SAM"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg253_2"
                     /sec_met_domain="Radical_SAM (E-value: 7e-15, bitscore:
                     44.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSTKRTKSSFRTMSTNRKLESVFLFVTGRCNAKCKMCFYAEDMDK
                     KEKDLTFEEIKTISETAGQFNRLWVSGGEPTLREELPEIIEMFYKNNEIKDVNIPTNGL
                     KPDRIVAWVKQIRQNCPELNINLSLSLDGFGDTHDIQRGVPGNFYKAMGTLKLIEKNFA
                     DDGRVLKNLATVITKYNENEVEDLMIWMLGRFHLSNHIIEAARGMTRDDGVKVLTEPTL
                     RALQDKVVPIYNVYGERMAEETGGFRKPIARFFYLGFIRTLYNVRATNIDHPTPWGMDC
                     TAGETTLVIDYDGRFRACELREPLGNVKDYDCNIAKIMESDAMKQEIAAIGHGDKANCW
                     CTHGCWITSSLVFNPRKMITQVYKGYREMKKLNRPLNISEELLQSIERKYALDEQKLVE
                     LGVRN"
     CDS             2380..3225
                     /locus_tag="ctg253_3"
                     /transl_table=11
                     /translation="MEDNIPKFDLPVDFIVGNSITGEILNQYGRFPCKIKAGIFVLCVQ
                     GTVHATVNLTEFTIRKNDFITLPPGCFIQIHEVSDDVKLCFAGFSSVFVSHINYIKTIT
                     NYLPVIFDSPIMPLPEHISLLYQQAFDLLINAYSMPKTIENKEIIKAIFTIFMQGVIEL
                     YRNHTPYSNAPMTRNMEICREFVQLAMENYTKEHSVSYYAKRLNITLQHFCYVIKKVSG
                     RTALDILSNIIIMDAKAQLKSTDMPVKKIAFALGFDNLSFFNKYFRQHVGMTPQEYRES
                     "
     CDS             complement(3524..4279)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_8"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg253_4"
                     /sec_met_domain="Fer2_3 (E-value: 3.4e-27, bitscore: 83.2,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_8 (E-value: 1.1e-12, bitscore: 36.8,
                     seeds: 153, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.2e-06, bitscore: 17.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDIKVTLKVWRQKSPKDKGQFETYQIDKINQSVSFLEMLDILN
                     ERLVSEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYMRRFNDGETITIE
                     PWRSAGFPVIRDLMVDRYAYDKIIQAGGFVSVNTGGVPDANSIAIPRADAEMAMDAAAC
                     IGCGACAAACKNGSAMLFVSAKVSQLALLPQGKVEAARRAKAMVAKMDELGFGNCTNTR
                     ACEMECPKNISVSNIARLNREFICAKLQD"
     CDS             complement(4307..5890)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 131.1; E-value:
                     1.9e-40)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg253_5"
                     /sec_met_domain="FAD_binding_2 (E-value: 1.5e-41, bitscore:
                     131.1, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 2.6e-39,
                     bitscore: 122.6, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="SNAIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQTGQ
                     QLLLGAYSALSRMIGLNKVKMYTRHEMLDVVKVDGRARGIIARNLITGQIERFSAHAVV
                     VATGGYVNTFFLSTNAMASNGSAAWQCYKKGAYFANPCMVQIHPTCVPVKGDYQSKLTL
                     MSESLRNDGRIWVPKKLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDVAS
                     RAAKERCDAGFGVNNTGLAVFLDFSDAINRLGKDVVKAKYGNLFDMYEEITNDDPYETP
                     MMIYPALHYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYFVL
                     PYTIQNYLSDQIQVPRFSTDLPEFAEAEKGIKDRIKKLMSIKGKESVDSIHKKLGHIMW
                     EHVGMARDAKGLQEAIQMLKELKKEFWSNVYIPGEADNLNVELDKALRLADFIEIGTLM
                     AHDALDRAESCGGHFRTEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNYQF
                     VVPQTRNYKK"
ORIGIN
        1 ctaatactat tatgaaaaaa ccacgatgta aatatactgc ttaatccaaa cctgtcaaga
       61 ggttttatgc taaatggtgt ttcatacact ataagaaaag ctaacaaggt tcaatatgtt
      121 tgttttttgc ttccaaagtg ttaaaataaa cactttttgt tcctatttct tctttaatat
      181 acgttgtgca cagtcggaca ataccacgat cgctccggct atgattgccg tatcttttat
      241 taccagccga cctgctccgg tcagtaaggg gaaaccatgt tctccgcttc ccaaatcggg
      301 cacccatact tcaggtgttg tcaccaggaa tgaaagcgta cccaatgtca ttatgatcgc
      361 cagtccggaa cctatcaagc caattttcgg agaaaagata cccaggaaag tcaggatacc
      421 gatcgccatg atcataatgc ctagtccatg tgaaaaaata taggtattgt tttctacatg
      481 ccactgatgc ttggcttcat tgaattcacc ttctttcaac ttatattctt tgtattccgg
      541 tgcactcttt gcatagaaaa agctcataaa cggactgttt gccacaaaag gaacgatccc
      601 ttccgcttca taattataga atttcagccc gccgatccat acgaatataa tcaggatagc
      661 tacgcggatc aggttaatac ccagtttctg tgaagaagct gcaatgttca gaaatgtgtc
      721 gaataaatct tttaatttag tagtcatgtc tttctttgtt tatcagttta acttatgatg
      781 caaagatccg acatttttcc cctatgggaa atggcaattc taccagataa tttgacaatt
      841 attcccttaa acacaataaa gccggttaac agaggtatat ttcccgtcaa ccggctttat
      901 aaatgatccg ttatcggaac tattttaatt acgaaccccc agctccacca gtttctgttc
      961 gtccaaggca tatttgcgtt cgatgctctg caaaagctct tcactgatat tcagaggacg
     1021 gttcagcttc ttcatctccc ggtatccttt atagacctgc gtgatcatct tgcggggatt
     1081 gaaaacaagc gatgaagtga tccaacaccc atgcgtacac cagcagttag ccttgtcacc
     1141 atgaccgata gcggcaatct cctgtttcat ggcatcgctc tccataatct ttgcgatatt
     1201 gcaatcataa tcctttacat taccgagcgg ttcgcgaagt tcgcaggcac ggaaacgacc
     1261 gtcatagtct attaccagtg ttgtttcgcc ggccgtacaa tccatacccc agggggtcgg
     1321 atgatcgata ttggtcgcac gtacattata taatgtacgt atgaaaccca ggtagaagaa
     1381 acgggcgata ggttttctga acccaccggt ttcctccgcc atacgctctc catatacatt
     1441 ataaatagga acaaccttat cctgcaaggc gcgtaaagta ggctcggtaa gaaccttcac
     1501 tccgtcgtcg cgggtcatgc cgcgtgcagc ttcgataata tgattggaaa gatggaaacg
     1561 tcccagcatc catatcatca gatcctcgac ctcattctcg ttatatttgg tgattacggt
     1621 ggccaggttt ttcaacaccc tgccgtcgtc agcaaagttt ttttcaatca gcttcagtgt
     1681 ccccatcgct ttgtagaaat tgccgggaac gccgcgctgt atatcatgcg tatcaccgaa
     1741 accgtccaaa gaaaggctca ggttgatatt cagttccgga caattctggc ggatctgttt
     1801 tacccaggcg acgatccggt caggcttcaa tccgttggta gggatattca cgtcctttat
     1861 ctcgttattc ttgtagaaca tctctataat ttcaggtaac tcctcacgca gtgtcggttc
     1921 accgccggat acccacaaac ggttgaactg cccggctgtt tcggagattg tcttgatttc
     1981 ttcaaaagtc aggtctttct cctttttatc catgtcttct gcatagaaac acattttaca
     2041 tttagcatta caccgtcctg tgacaaacag gaaaacagat tctaattttc tgtttgtgct
     2101 cattgtcctg aaagagctct ttgttctttt cgtactcatt gttattatcg attagatttc
     2161 tctattagta ttagctccca tatcttattt agcagataga caaatatata ccatcccaac
     2221 aaatctatca gcaagacttg aaaacattcg caaatttcaa ctattataca gcacaaaaca
     2281 atctccattt gtgggattcg atgttctata tttaagatta tcgactaact tgtctcaaaa
     2341 aaactatctt tgtacattca attaagtgta tataaaagta tggaagacaa tattcctaaa
     2401 ttcgacctcc ccgtagattt tattgtagga aacagtatta caggtgaaat actgaaccag
     2461 tacggacgtt tcccgtgcaa gatcaaggca ggcatattcg tgctttgcgt acaagggaca
     2521 gtccatgcga ctgtaaactt aactgagttt accatccgga aaaacgactt tatcaccttg
     2581 cccccgggtt gcttcattca gatacatgag gtttccgatg atgtaaaact gtgttttgca
     2641 ggcttttctt ccgtattcgt cagccatatc aattatataa agacgattac gaactatctg
     2701 cctgtcatat tcgacagtcc gatcatgcct ttgccggagc atatatcctt actctaccaa
     2761 caggctttcg acttgctgat caatgcttat tccatgccta aaaccatcga aaacaaggag
     2821 attatcaagg ctatctttac tatcttcatg caaggggtta ttgaattata caggaatcat
     2881 acgccatata gcaatgctcc catgacgcgt aatatggaaa tatgccggga gttcgtacag
     2941 ctggcgatgg aaaactacac gaaagagcat agcgtgtctt attacgccaa aaggctcaac
     3001 atcaccttgc agcatttctg ttatgtgata aaaaaggtca gcggacggac tgcattggat
     3061 atcctgagca atatcatcat tatggatgcc aaagcgcagc ttaaatcgac cgatatgccg
     3121 gtcaagaaaa ttgcattcgc cctgggcttc gacaaccttt cgttcttcaa caaatatttc
     3181 cggcaacatg taggaatgac tccgcaggaa tacagggaga gctaaaaaaa gggatatgcc
     3241 ggacgcaaac ccctataaac aattaaagcc tgtctcacga caagccctaa ccaactttgt
     3301 taaccttaaa tctaatacta ttatgaaaaa accatagtac aaagatacag gtattctcca
     3361 acttgtcaat agttaaaatg ctaattagtg ttgtatagca tataagaaac actaacaaca
     3421 ttcaagatat ttatctatga cttcagaaat gttaaaatag gggcaaaaaa gaagaggcgc
     3481 cgccataagc aacgcctctc cggacataca tatataatgt atattagtct tgcaatttag
     3541 cacaaatgaa ctcgcggttc aaacgagcga tgttgctgac agagatgttc ttcggacatt
     3601 ccatttcgca agcacgcgtg ttggtacagt taccgaagcc cagttcgtcc atcttggcaa
     3661 ccattgcttt cgcacgacga gcagcttcta ccttaccctg aggcaacaga gccaactggc
     3721 taacctttgc agaaacgaac aacatagccg aaccgttctt acaagcagca gcacaagcac
     3781 cgcaaccgat acatgcagca gcatccatag ccatttcagc atctgcacga ggaatagcga
     3841 ttgaattggc atcaggtaca ccacctgtgt tcactgatac gaaaccacct gcctggataa
     3901 ttttatcgta agcataacgg tctaccatca agtcgcggat taccgggaaa ccggcagaac
     3961 gccacggttc gattgtaata gtttcgccat cattgaagcg acgcatataa agctggcaag
     4021 tagttgcgcc ggttgcaggt ccgtgcggat gtccgtttac atacaaagaa cacataccgc
     4081 agataccttc acgacagtcg tgatcgaata cgatcggctc tttaccttca ctgaccaaac
     4141 gttcattcaa aatgtctaac atttccagga aagaaacact ctgattgatt ttgtctattt
     4201 ggtaagtctc aaattgtcct ttatctttcg gacttttctg acgccaaact ttaagtgtta
     4261 cttttatatc tttatccatg attctaattt ttgtgattga tgattattac ttcttgtagt
     4321 tacgtgtttg aggaacaacg aactgatagt tcaaatcttc cttcagcata accggatctt
     4381 tgtcttcacc ctggtattcc caacaagata cgtacatgaa cttatcgtca tgacgcaggg
     4441 cttcgccttc ctcggtctgg tgctctgtac ggaagtgtcc tccacaagat tcggcacggt
     4501 ccaatgcatc gtgtgccatc aatgtaccaa tctcaataaa gtcagccaaa cgcaatgcct
     4561 tatccaactc tacgttcagg ttatcggctt cacccggaat atatacatta ctccagaact
     4621 ctttcttcag ctctttcaac atctggatcg cttcctgcaa accttttgca tcacgtgcca
     4681 tacctacatg ttcccacatg atatgaccca gtttcttatg gatagaatct accgactctt
     4741 tacccttgat actcatcaat ttcttgatgc ggtctttgat ccccttttca gcttctgcaa
     4801 actcaggcag gtcggtactg aaacgcggaa cctggatctg gtctgacaga tagttctgga
     4861 ttgtataagg caatacaaag tagccatcgg ccaatccctg catcaacgca gaagcaccca
     4921 gacggttagc tccgtggtca gagaagttag cttcaccgat agcgaacaga cccggaatag
     4981 aagtcatcaa ttcatagtca acccacagac cacccatcga gtagtgcaat gcaggataaa
     5041 tcatcatcgg agtttcgtat ggatcgtcgt tggtaatttc ttcgtacatg tcgaacaagt
     5101 taccatattt agcttttaca acatctttac ccagacggtt gatggcgtcg gagaagtcga
     5161 ggaacacggc cagacctgtg ttgttcacac cgaagccggc gtcgcaacgt tctttagcgg
     5221 cacgtgaagc cacgtcacgc ggaaccaggt taccgaatgc cggataacgg cgttccagat
     5281 agtagtcgcg gtctgcttca ggaatgtctt tccctttctt agttccggcc tgtaatgctt
     5341 ttgcatcttc cagtttcttc ggaacccaga tacgaccgtc gttacgcaat gattcagaca
     5401 tcaaagtcag cttagactgg tagtcacctt tcaccggtac acaagtcggg tggatctgca
     5461 ccatacaagg gttagcgaag taagcacctt tcttgtagca ctgccatgca gcagaaccgt
     5521 tggaagccat tgcgttagta gacaggaaga aagtatttac ataaccacct gttgccacca
     5581 ctactgcgtg agcagagaaa cgttcgatct gacctgtgat caggttacgt gcgatgatac
     5641 cgcgggcacg gccatccacc ttcacaacgt ccagcatttc gtggcgggta tacatcttta
     5701 ctttgttcaa accgatcata cggctcaaag cagagtaagc acccagcaac aactgctgtc
     5761 cggtttgacc gcgggcatag aatgtacgtg atacctgagc accaccgaat gaacggttat
     5821 ccagcaaacc accgtattcg cgagcgaaag gaacaccctg agctacgcac tggtcgatga
     5881 tagcatttga
//
