LOCUS       c00279_gnlPro..         3001 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|GKNAIOEI_279.
ACCESSION   c00279_gnlPro..
VERSION     c00279_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 11:59:25
            Original ID  :: gnl|Prokka|GKNAIOEI_279
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3001
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..504
                     /locus_tag="ctg279_1"
                     /transl_table=11
                     /translation="SLFPVKKAAPAEDAPILIAPFSRLGSASEWSWEQWRELVSLLPGR
                     TVLAALEEDRERAASLAEHLNVELAVGAPEALFPVMDGAAAAVAVDGDLPSLCSFRGLP
                     VVTLFSTRLPDVYRPMGTFNRSLYSHQCCSPCFRKECDRDVPCNRHIAVRDVLEALREI
                     SGKE"
     protocluster    1..3001
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[752:3000]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      753..3000
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3001
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3001
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(753..1532)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 53.7;
                     E-value: 9e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg279_2"
                     /sec_met_domain="Radical_SAM (E-value: 4.1e-32, bitscore:
                     99.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELHPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRQGGFPDCVH"
     CDS             complement(1549..3000)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg279_3"
                     /sec_met_domain="Gly_radical (E-value: 1.7e-46, bitscore:
                     145.0, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 1.3e-173, bitscore:
                     566.2, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="NGAAMSLGRVSTFFDIYFTRDLEQGLITEEEVQEIIDQFVMKLRI
                     VRFIRTPDYNNLFSGDPTWVTESIGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTV
                     LWSRNLPEAFKSFCAKVSIETSSVQYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFG
                     ARANLAKCLLYALNGGVDELKGKQVAPPSPRYTEEILNYDEVMALYDKMQDWLAKTYID
                     ALNIIHYMHDKYCYERIEMALHDPEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEE
                     GLIVDFKTEGEFPCYGNNDPRVDDIACSLVSNFMEKLRRLHTYRNSLPTQSILTITSNV
                     VYGKKTGNTPDGRRAGEPFAPGANPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSI
                     VPQALGKEERERRVKLVSLLDAYFAATGHHINVNVLERKTLLDAMDHPEKYPQLTIRVS
                     GYAVNFIKLTREQQQEVINRTFHTR"
ORIGIN
        1 tctttgttcc ctgtgaaaaa agcggccccg gcggaagatg cccccatttt aatcgctccg
       61 ttttcccgcc tggggagcgc cagtgaatgg agctgggagc agtggaggga gctggtttcc
      121 cttctgccgg gacggacggt gctggcggct ctggaagaag accgggagcg cgccgcgtcc
      181 ctggcggaac atttgaatgt ggagctggcg gtgggtgcgc cggaagctct ctttcccgtc
      241 atggacggcg ctgcggcggc agtagccgtg gatggcgact tgccgtccct ctgttctttc
      301 cgggggcttc cggtggtgac tctgttttcc acccggcttc cggatgttta ccgccctatg
      361 ggcacattca accgttccct ttacagccac cagtgctgtt ctccctgctt ccggaaggaa
      421 tgtgaccggg atgttccctg caaccgccac attgccgtgc gggacgtact ggaagccttg
      481 cgggaaattt ccgggaaaga ataatctggg aagggacgga atgccgtccc ccggcgttgg
      541 cggaaaaaac ggcgggaatg ctgccgttgc cgtaaagaat gatgaaataa cgaaaggaat
      601 tgcggaaatg ccgtattccg gagcaattcc agaaatgcgt cttccttttt gcatggattc
      661 tgcagtggag gggagagggc ccctcatccg gaagaggggg atgcctgcgt ccggtttgct
      721 gcggttggtg gcgccaaagc gcgaaaatcc ggttaatgca cgcaatccgg aaagccgccc
      781 tgccggaaaa tgccgcggac ctgttccgcc agttctgcgg acgcttccgg gacgtccttc
      841 aaggtatagt ccagcttcaa ttcatcccat ttgaatctcc ccagctggtg gaaagggagc
      901 acgtccacgc gttccacgtt gcccagaagc gccgcatggc gggagagttc ctcaatttct
      961 tccagattgt ccgtaacgcc aggtaccagc acatacctca gccacacggg cttgcggagg
     1021 gaggccaggc gttccgcaaa ctccagtgta gggtgcagtt ccccgcctgt cagggcgcga
     1081 taccgtttcg gattccatgc cttgatatcc agcaggacca gatccgtatc cgccagcatt
     1141 tcttcgcccg cattcacgcc cagatggccg gaggtgtcca ggcaggtgtg caggcccagt
     1201 ttccggcagc ctttcagcac ggcaccggca aaatccggct ggaagagggg gtccccgccg
     1261 gagagggtga cgccgccgcc cgccgcctgc aaaaaatccc ggtaacgggc tatttccttg
     1321 aggacatcat ctgcgctcct gagccggccc ctccgcacgt aggaggtatc cggattatgg
     1381 cagtagcggc agcgcaggct gcaaccggac aggaaaagga cgaagcggat gccggggccg
     1441 tccacggtgc cgcaggattc cacggaatga accaaaccgg tgacggagct ttctccgtcc
     1501 ggatcatggt tttgtggaaa aacctgcggc atccgcttgc agtccgggtt aacgggtgtg
     1561 gaaagtgcgg ttgatgacct cctgctgctg ttcccgggtc agcttgatga aattcacggc
     1621 atagccggaa acgcggatgg taagctgcgg gtatttttcc gggtgatcca tggcgtcgag
     1681 aagcgttttc cgttccagta cgttcacatt aatgtggtga cctgtagcgg caaagtaggc
     1741 gtccagcagg gagacgagct tgacgcggcg ttcacgttct tccttgccca gggcctgagg
     1801 aacgatggag aaggtgtagg agatgccgtc cagggagtcg tcataggaca gcttggctac
     1861 ggagagcatg gaggccacgg ctccgttcct gtcccttccg tgcatggggt tggctccggg
     1921 ggcgaacggt tccccggctc ggcggccgtc cggcgtgttg cccgtctttt tgccgtacac
     1981 cacgttggag gtgatcgtca ggatggactg ggtgggcagg gaattgcggt aagtgtggag
     2041 cctgcgcagt ttttccatga aattgctgac caggctgcat gctatgtcgt ccacgcgcgg
     2101 gtcattgttc ccgtaacagg ggaattcccc ttccgtcttg aagtccacaa tcagcccttc
     2161 ttcattgcgg atggctttta cggtggcata cttgatggcg gacagggagt ccgccgccac
     2221 ggaaagcccg gcgattcccg tagccatcgt gcgcagaatt tccggatcat gcagcgccat
     2281 ttcaatgcgt tcatagcagt atttgtcgtg catgtagtgg atgatgttca gcgcgtcaat
     2341 gtaagttttg gcgagccagt cctgcatctt gtcgtacagg gccatcacct catcatagtt
     2401 gagaatttcc tccgtgtagc ggggggaggg cggggccacc tgcttgccct tgagttcatc
     2461 cacgccgccg ttcagggcgt agagaaggca cttggcgagg ttggcgcgcg ctccgaagaa
     2521 ctgcatctgc ttgccgatgc gcatggcgga cacgcagcat gcgatgccgt agtcgtcgcc
     2581 ccaatgcggg cgcatcaggt cgtcgttctc gtattggacg gaggacgttt caatggaaac
     2641 cttggcgcag aagcttttga aggcttccgg caggttccgg gaccacagaa cggtcaggtt
     2701 cggttccgga gccggcccca ggttgtacag ggtctgcagc atgcggaagg aactgcgggt
     2761 gacgagggtg cgcccgtcct ctcccatgcc gccgatggat tccgtcaccc aggtggggtc
     2821 tccggagaac aggttattgt aatcaggcgt gcggatgaaa cggacgatgc gcagcttcat
     2881 cacgaactgg tcgatgattt cctgaacttc ctcttccgtg atgagcccct gttccaggtc
     2941 gcgggtgaaa taaatgtcga agaaagtgga aacgcggccc agggacatgg ccgctccatt
     3001 c
//
