LOCUS       c00423_gnlPro..         4164 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|ADJPAMPF_423.
ACCESSION   c00423_gnlPro..
VERSION     c00423_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 19:21:26
            Original ID  :: gnl|Prokka|ADJPAMPF_423
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4164
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             1..270
                     /locus_tag="ctg423_1"
                     /transl_table=11
                     /translation="DLGSLQIGLNSEGAASVKEFSGSMIKGAHLIFRPGKTLAELMKIL
                     SYQQVQTRAVAQAGAGDGGGEDPDKNPDSGGDGSGDEEAPDPTV"
     protocluster    1..4164
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[925:3875]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      926..3875
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4164
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4164
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             330..497
                     /locus_tag="ctg423_2"
                     /transl_table=11
                     /translation="MKEIVSRILDVIMYLIPFFGKRKRDRVVREVRYHTTCKEVCKVKT
                     TEREKEHEKD"
     CDS             484..933
                     /locus_tag="ctg423_3"
                     /transl_table=11
                     /translation="MRKISLIVIHCSATRVDCDFTAKDVDTAHRYRGFSCWGYHYYIRK
                     SGEIEPMRDKDTVGAHARGYNAISLGVCYEGGLDENGKAADTRTPRQKEALHRLVHELL
                     QRYPEAKVVGHRDLSPDTNYNGIVDPWERIKECPCFEVIGEFSIN"
     CDS             complement(926..1633)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 64.7;
                     E-value: 3.5e-20)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg423_4"
                     /sec_met_domain="Radical_SAM (E-value: 2.7e-24, bitscore:
                     74.8, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MMINVHSYESMGTFDGPGLRLVVFLQGCNFRCLYCANPDTIAGKG
                     GTPTXXXXQRPFFGKRGGITFSGGEPTFQAKALVPLVRELKEKGIHVCLDSNGGLWNED
                     VEELFRLTDLVLLDIKEFNPNRHQTLTGRSNEQTIRTAAWLEEQEKPFWLRYVLVPGYS
                     DFEEDIRALGEALGKYKMIQRVEILPYHTLGVHKYEAMGQEYKMKGVKENTPEQLEKAA
                     EVFKEYFTTVVVN"
     CDS             complement(1647..3875)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate1"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg423_5"
                     /sec_met_domain="Gly_radical (E-value: 3.1e-47, bitscore:
                     148.1, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate1 (E-value: 0.0, bitscore:
                     1173.8, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MELNKIFKDGLWSSEINVRDFVSHNITPYYGDASFLEGPTERTKA
                     VWNRCLEALAEERENNGVRSLDNVTVSTITSHKAGYIDKENELIVGLQTDELLKRAIKP
                     FGGINVVSKACHENGVEVDDRVKDIFTHYRKTHNDGVFDVYTEEIRSFRSLGFLTGLPD
                     NYARGRIIGDYRRMALYGIDRLIEAKKEDLHNLTGPMTEARIRLREEVAEQIKALKDMK
                     VMGEYYGLDLSRPAYTAQEAVQWVYMAYLAAVKEQDGAAMSLGNVSSFLDIYLEYELSK
                     GTITESFAQELIDQFVIKLRMVRHLRMQSYNDIFAGDPTWVTESLGGRLNDGRTKVTKT
                     SFRFLQTLYNLGPSPEPNLTVLWSPELPEGFKEFCAKVSIDTSSIQYENDDLMREVRQS
                     DDYGIACCVSYQEIGKQIQFFGARCNLAKALLLAINGGRCENTGTVMVKNIPVLTSDTL
                     KFEEVMDNYKKVLTEIARVYNEAMNIIHYMHDKYYYEKAQMALVDTNPRINLAYGVAGL
                     SIALDSLSAIKYAKVTARRNDIGLTEGFDIEGEFPCFGNDNDKVDHLGVDLVYFFSEEL
                     KKLPVYKNARPTLSLLTITSNVMYGKKTGATPDGRAKGVAFAPGANPMHGRDKNGAIAS
                     LSSVAKLRYRDSQDGISNTFSIVPKSLGATDEDRIENLVTMMDGYFTKGAHHLNVNVLN
                     RDMLYDAMEHPENYPQLTIRVSGYAVNFVKLSREHQLEVISRSFHERM"
ORIGIN
        1 gatttgggca gtttgcagat aggactcaac agtgaaggtg cggcatcggt caaggaattt
       61 tccggttcaa tgattaaggg ggcgcatctt attttccgcc ccggtaagac gctggcggag
      121 ttgatgaaga ttctgagtta tcagcaagtg cagactcgtg cggtggcgca ggcgggtgca
      181 ggcgatggcg ggggagaaga ccctgacaag aatccggatt ccggtggtga tggctccggt
      241 gatgaagagg ctccggaccc tacggtataa ttggcagacg ggtcaaggta ataacgaatt
      301 agaataactt ccattaatat tttgataaga tgaaagaaat agtaagtcgg attttagatg
      361 tgatcatgta tctgattcct ttcttcggaa agagaaagcg ggacagggtg gtgagagaag
      421 tgcgctatca tactacttgt aaggaggtgt gcaaagtgaa aacgacggaa agggaaaagg
      481 aacatgagaa agattagtct gattgtgatc cattgttccg ccacccgtgt cgattgcgac
      541 tttacggcaa aggatgtgga tacggctcac cgttatcgtg gcttttcgtg ctgggggtat
      601 cattattata tacgcaagtc gggcgagatt gagccgatgc gggataaaga tacggtcgga
      661 gctcatgcac gtggctataa tgcgatcagc ttaggtgtat gttatgaggg cggtctggat
      721 gagaatggaa aagctgcgga tacgcgtaca ccccgtcaga aggaggcgct acatcgcttg
      781 gtacacgaat tgttgcagcg ttatccggaa gctaaagtag tagggcatcg ggatttaagt
      841 cccgacacca actataatgg gatcgtagat ccttgggaac ggattaaaga atgcccttgc
      901 tttgaggtca ttggagaatt ctccattaat taactaccac cgtagtgaaa tactctttga
      961 atacctccgc agctttttca agctgttcgg gggtattttc ttttacccct ttcatcttgt
     1021 attcctgtcc catcgcttcg tacttatgta cgcccagtgt atggtaaggc agtatttcca
     1081 cccgttggat catcttgtat tttccgagtg cttctcccag tgcgcgtatg tcttcttcaa
     1141 aatcgctgta tccgggcact agcacgtagc gtagccagaa aggtttctcc tgttcttcca
     1201 gccaggcggc cgtgcggatg gtttgctcgt tgcttcttcc ggtgagcgtt tgatggcggt
     1261 tagggttgaa ttcctttata tccaataaca caagatcggt cagtctgaat aattcttcta
     1321 cgtcttcgtt ccaaagtcct ccgttgctgt ccagacagac atggatgcct ttttctttca
     1381 gttcgcggac cagcgggacg agtgctttcg cctgaaacgt cggttcgcct ccggagaaag
     1441 tgattcctcc tcgtttcccg aagaaggggc gctggnnnnn nnnnngggta ggcgtgcctc
     1501 cctttcctgc tatcgtatcc ggattggcgc aatagagaca acggaagttg catccttgaa
     1561 gaaaaacgac gagccgtaag cccggcccgt cgaatgttcc catactttcg tatgaatgta
     1621 cgtttatcat catatagagt aatagattac atacgttcgt ggaagctacg gctgataact
     1681 tccagttggt gttcgcggct cagcttcacg aagtttacgg catagccgga aacgcggata
     1741 gtcagctgcg gatagttttc cggatgctcc atggcatcgt acagcatatc acggttcagt
     1801 acgtttacgt tcaggtggtg ggcacctttg gtgaagtaac cgtccatcat cgttacgagg
     1861 ttctcgatac ggtcttcgtc cgtagcaccc agtgatttcg gaacgataga gaaggtgttg
     1921 ctgataccgt cctgtgagtc acggtaacga agtttcgcta cagaactcag agaagcgatc
     1981 gcaccattct tgtcgcgtcc gtgcatcggg ttggcacccg gagcgaaggc aacacctttg
     2041 gcacgtccgt cgggagtagc accggtcttt ttgccgtaca tcacattgga agtgatagtc
     2101 agcaaggaga gggtaggacg ggcattcttg taaacaggca atttcttcaa ttcttcgctg
     2161 aagaagtata ccaggtctac gccaaggtgg tctaccttgt cgttgtcgtt accgaagcaa
     2221 gggaattctc cttcgatgtc gaagccttct gtcagaccga tatcattgcg gcgggcagtt
     2281 actttcgcat atttgatggc agacagtgaa tcgagggcga tggaaagtcc ggctacaccg
     2341 taagctaggt tgatgcgcgg gttcgtatct acaagagcca tttgggcctt ctcgtagtaa
     2401 tacttgtcgt gcatatagtg gatgatgttc atcgcttcgt tatatacgcg ggcgatttcg
     2461 gtcagcactt ttttgtagtt gtccattact tcttcgaact tcagcgtgtc actggtcagt
     2521 acggggatgt tcttcaccat gactgtaccg gtattctcgc aacgtccgcc gttgatggca
     2581 agcaacaggg ctttggccag gttgcaacgg gcaccgaaga actgaatctg cttacctatt
     2641 tcctggtatg atacgcagca ggcgattccg tagtcgtcgg actgacgtac ttcgcgcatc
     2701 aggtcgtcat tttcgtactg gatagaagaa gtgtcgatag aaacctttgc acagaattct
     2761 ttgaatcctt ccggaagttc cgggctccag agtacggtca ggttcggttc cggtgaaggg
     2821 ccgaggttgt acaatgtctg caggaaacgg aaggaggtct ttgtcacttt ggtacgtccg
     2881 tcgttgagac gtccgcccag agattcggtt acccaggtcg ggtcgcctgc aaagatgtcg
     2941 ttgtatgatt gcatacgcag gtggcggacc atacgcagtt tgatgacaaa ctggtcgatc
     3001 agctcttgcg cgaatgattc ggtgatggtt cctttgctca gttcatattc cagatagata
     3061 tcgaggaaag aagaaacgtt acccagtgac atggcggcac cgtcttgttc tttgacggcg
     3121 gcaaggtaag ccatatatac ccactgtacg gcttcttgtg ccgtgtaagc gggacggctc
     3181 aggtcgagac cgtagtattc gcccatcact ttcatgtctt tcagtgcctt gatctgttct
     3241 gccacttctt cgcgcaggcg gatgcgggcc tctgtcatcg gaccggtgag gttgtgcaaa
     3301 tcttccttct ttgcttcgat cagacggtcg atgccgtaaa gtgccatacg gcggtagtca
     3361 ccgatgatgc gtccgcgtgc ataattgtcg ggaagtccgg taaggaatcc cagcgaacgg
     3421 aaggaacgga tttcttccgt atatacgtcg aatactccgt cgttgtgcgt cttgcggtag
     3481 tgagtgaaaa tatcttttac gcggtcgtcc acttccacgc cgttttcgtg acaggctttg
     3541 ctgactacgt tgatacctcc gaaaggcttg atggcacgtt tcagaagttc gtctgtctgc
     3601 aggccgacga tcagttcgtt ttccttgtcg atatatccgg ccttgtggga agtgatggtt
     3661 gatacggtaa cattatctaa tgagcggaca ccgttgtttt ctctttcttc tgccagtgct
     3721 tcgaggcagc ggttccatac ggctttggta cgttctgtag gtccttcgag gaatgaagca
     3781 tctccgtaat acggagtgat gttatgactt acgaaatctc tgacgttgat ttcgctgctc
     3841 caaagaccgt ctttaaagat cttgtttaat tccataaaat gataaaatta gaggttgtag
     3901 ttatcctatt ctttagagcg cacaaagtta caatcatttt tcatataatc agcataaata
     3961 tgctctattt tatgcttttc aacataaaat acctattaat tgctatcctt aggaaaagat
     4021 tagattaaat ttttagtgaa aactcttgca agtttaaaat atcttcgtac ctttgcaccg
     4081 cttttaacga aaagcacttc tgataaggaa gttttggaga ggtggcagag tggtcgattg
     4141 cggcggtctt gaaaaccgtt gtac
//
