LOCUS       c00067_gnlPro..         3424 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|IMMOBLNO_67.
ACCESSION   c00067_gnlPro..
VERSION     c00067_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 13:21:57
            Original ID  :: gnl|Prokka|IMMOBLNO_67
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3424
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3424
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:2694]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..2694
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3424
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3424
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(3..410)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 54.4;
                     E-value: 5.5e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg67_1"
                     /sec_met_domain="Radical_SAM (E-value: 3.1e-22, bitscore:
                     67.4, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNRAPDGKNSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSADDVLREIARYRDFLQAAGGGVTLSGGDPLFQPDFTGA
                     VLKGCRKLGLHTCLDTSGHLGANADEKMLADT"
     CDS             complement(427..2694)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg67_2"
                     /sec_met_domain="Gly_radical (E-value: 1.3e-46, bitscore:
                     145.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 4.7e-282, bitscore:
                     924.3, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MISIVKDLTESTALPQEWRGFKPGAWTESIDVRDFIQHHYTPYSG
                     NEEFLSGPSQRTLRLWDELKVLLKREIDNGGVLDADEEVVSSITSHKPGYIDKELEVVV
                     GLQTDAPLKRALMPFGGLRMAQQALESYGFKMCEKTADIFKKIRKTHNEGVFDSYTSDI
                     RAARSAGIITGLPDAYGRGRIIGDYRRVALYGTDRLIAERRKDLKNREHSPLTDELIRL
                     REEMSEQIRALEELAQLGASYGCDLTRPAANAREAVQWTYLGYLAAVKEQNGAAMSLGR
                     VSTFFDIYFTRDLEQGLITEEEAQEIIDQFVMKLRIVRFIRTPDYNNLFSGDPTWVTES
                     IGGMGEDGRTLVTRSSFRMLQTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETSS
                     VQYENDDLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGK
                     QVAPPSPRYTEEVLHYDEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHD
                     PEILRTMATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVD
                     DIACSLVSNFMEKLRKLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRSGEPFAPGA
                     NPMHGRDRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAY
                     FAATGHHINVNVLERETLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTFH
                     TR"
     CDS             3244..3423
                     /locus_tag="ctg67_3"
                     /transl_table=11
                     /translation="MLTILLVVYCLVFSASSVPAPVLPATLKPEPHQRDPYDWNARHEA
                     VKKRNRTVKPEYVMI"
ORIGIN
        1 tccgtatccg ccagcatttt ttcgtctgca ttcgcgccca gatggccgga ggtgtccagg
       61 caggtgtgca gcccaagctt ccggcagcct ttcagcacgg cgccggtaaa atccggctgg
      121 aagagggggt ccccgccgga gagggtaacg ccgccgcccg ccgcctgcaa aaaatcccgg
      181 taacgggcta tttccctgag gacatcatcc gcgctcctga gccggccccg ccgcacgtag
      241 gaggtatccg gattgtggca gtagcggcag cgcaggctac agccggacag gaaaaggacg
      301 aagcggatgc cggggccgtc cacggtgccg caggattcca cggaatgaac caaaccggtg
      361 acggagtttt tcccgtccgg agcacggttt tgtggaaaaa cctgcggcat ccgtttgtct
      421 ttcgggttag cgggtgtgga aggtgcggtt gatgacttcc tgctgctgtt ccctagtcag
      481 cttgatgaaa ttcacggcat agccggaaac gcggatggta agctgcgggt atttttccgg
      541 gtggtccatg gcgtcgagaa gcgtttcccg ttccagtacg ttcacattaa tgtggtggcc
      601 tgtagcggca aagtaggcgt ccagcaggga gacgagcttg acgcggcgtt cccgttcctc
      661 cttgcccagg gcctgcggaa cgatggagaa ggtgtaggag atgccgtcca gggagtcgtc
      721 ataggacagc ttggctacgg agagcatgga ggccacggct ccgttcctgt ccctgccgtg
      781 catggggttg gcgccgggag cgaacggttc cccggagcgg cggccgtccg gcgtgttgcc
      841 cgtctttttg ccgtacacca cattggaggt gatcgtcagg atagactggg tgggcaggga
      901 attgcggtaa gtgtggagct tgcgcagttt ttccatgaaa ttgctgacca ggctgcatgc
      961 tatgtcgtcc acgcgcgggt cattgttccc ataacagggg aattcccctt ccgtcttgaa
     1021 gtccacaatc agcccttctt cattgcggat ggctttcaca gtggcgtact tgatggcgga
     1081 cagggagtcc gccgccacgg aaagcccggc gattcccgtg gccatcgtgc gcaggatttc
     1141 cggatcatgc agcgccattt caatgcgttc atagcagtat ttgtcgtgca tgtagtggat
     1201 gatgttcagc gcgtcaatgt aagttttggc gagccagtcc tgcatcttgt cgtacagggc
     1261 catcacttca tcatagtgga gaacttcctc cgtatagcgg ggggagggcg gggccacctg
     1321 cttgcccttg agttcatcca cgccgccgtt cagggcgtaa agaaggcact tggcgaggtt
     1381 ggcgcgcgct ccgaagaact gcatctgctt gccgatgcgc atggcggaca cgcagcacgc
     1441 gatgccatag tcgtcgcccc agtgcggacg catcaggtca tcgttctcgt attggacgga
     1501 ggatgtttca atggaaacct tggcgcagaa gcttttgaag gcttccggca ggttccggga
     1561 ccacagaacg gtcaggttcg gttccggagc cggccccagg ttgtacaggg tctgcagcat
     1621 gcggaaagaa ctgcgggtga cgagggtgcg gccgtcctct cccatgccgc cgatggattc
     1681 cgtcacccag gtggggtctc cggagaacag attattgtaa tccggcgtgc ggatgaaacg
     1741 gacgatgcgc agcttcatca cgaactggtc gatgatttcc tgagcttcct cttccgtgat
     1801 gagcccctgt tccaggtcgc gggtgaaata aatgtcgaag aaagtggaaa cacggcccag
     1861 ggacatggcc gctccgttct gttccttcac ggcggccagg tagcccaggt acgtccattg
     1921 cacggcttcg cgggcgtttg cagcggggcg cgtgaggtcg cagccatagg aggcgcccag
     1981 ctgggcgagt tcttccaggg cgcgtatttg ttcgctcatt tcctcacgca gacggatcag
     2041 ctcatccgta aggggggaat gttcacggtt tttgagatct ttgcggcgtt cggcaatcag
     2101 cctgtctgtg ccgtacaggg ccacccgtcg gtagtccccg ataatgcggc cgcggccgta
     2161 ggcgtccgga aggccggtga taatgccggc ggaacgggcc gcccggatgt cggaggtgta
     2221 ggagtcgaaa acgccttcat tgtgcgtctt gcgtatcttt ttgaaaatat ccgcggtttt
     2281 ctcgcacatc ttgaacccgt aggattccag ggcctgctgg gccatgcgca ggccgccaaa
     2341 gggcatcagg gcccgtttca ggggggcgtc cgtctggagg cctacgacca cttccagttc
     2401 cttgtcaatg tagccgggct tgtgggatgt gatggaggat actacttctt catcagcatc
     2461 cagtacgccg ccgttgtcta tttcccgttt cagcaggact ttcagttcat cccataaacg
     2521 aagcgtgcgc tgggaagggc cggaaagaaa ctcttcattg ccggaatagg gtgtataatg
     2581 atgctggatg aagtcccgta cgtcaatgga ctccgtccat gctcccggct tgaatccccg
     2641 ccattcctgc ggaagggcgg tggattccgt caagtctttt actatgctga tcataaatgg
     2701 aatgtagtga agaagaccgg agaaaaacgt tccgcaaccc ctgaaaggcc ggaaacggca
     2761 aaactccgcg ccgcgtaatt atatcgtgaa ttccggggga caacaaggct aattctttgt
     2821 gaacggaata tgggagcgtt ggcttggcat gtttcacctg tttgtccgtt gcggaacggg
     2881 agatgagggg ggaaatacga ttgcgggaaa gtggcggaaa caacgtattt ttctccaatc
     2941 ccggcattgc tgaagaaaat ggaccgcaag tacggcaaac tgccctctct ctgatggaag
     3001 tagaataaga ggggagatcg ctcctgttcc aggagaggcc gggagatgga tgaaatggaa
     3061 tgcattttgg aaaaggccgg acgcggtgtt tgcctggggt cttatgatgg cgggatttgc
     3121 cggacgggca gtggtctttt ttattccgcc ggcggaagtg ccgcctgcgg tcttgatcgc
     3181 ccgacgggat ttgcttaaaa tgaaaaccgg tttcactttt ttatcatgaa gttcctatcc
     3241 tgcatgctca ccatattgct ggtggtttac tgtctggtgt tttctgcatc ttccgttccg
     3301 gcccccgtgt tgcccgctac cctgaagccg gagccccatc aacgggaccc gtatgattgg
     3361 aatgcccggc atgaagccgt taaaaaaaga aaccggaccg tcaaacctga atacgtgatg
     3421 atag
//
