LOCUS       c00074_gnlPro..         7129 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PAHHILEL_74.
ACCESSION   c00074_gnlPro..
VERSION     c00074_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 15:30:15
            Original ID  :: gnl|Prokka|PAHHILEL_74
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 7129
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..7129
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[5144:7128]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      5145..7128
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..7129
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..7129
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             2..538
                     /locus_tag="ctg74_1"
                     /transl_table=11
                     /translation="GTGSYQARGNTTGAGFGAMYELAYDIALNEDRSVILQPLFNASIV
                     TTRMDGYRESGSAGNAGLKVEDQELTTAAVAVGARLSGLMGSNIFGREALGEVRVNVSQ
                     DMGDRRGQANVGFLADPSYTRPVYGAKVGSTAFQIGAGLSVPVGTQGVIFVDGNADIRS
                     GSSSINGSIGYRYNF"
     CDS             complement(660..1070)
                     /locus_tag="ctg74_2"
                     /transl_table=11
                     /translation="MTDHYYTSETENVGQFRCETTFIPSNVTFATDLPASFEGRAEFPS
                     PGVLLGSTLASCMMSLVSVVAARKGVDLKGMRVLARAAESSKGIEKIELKAIMPLEGSH
                     PLRAMLEKTALSCPVRQALRPDLETPIEWEWK"
     CDS             complement(1269..2432)
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Abhydrolase_1"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg74_3"
                     /sec_met_domain="Abhydrolase_1 (E-value: 2.5e-30, bitscore:
                     94.8, seeds: 48, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MQLPAVQTKFLDLPNSFSLRNGATLDKVRVAYEQYGTLTPNKDNA
                     ILLFHALSGSQHAYGYNPEVPGIGSLWKPENHEGWWNSIIGPGKPLDTNRFCIICANYL
                     GGCYGTTGPATPCPADGQPYGSRFPHVEAADQARLQALLLDSLGIERVHLMGPSVGGLI
                     ALSFACQFPERVRSFISIGSGYRASIEHRLSLFEQILAIELDPDFQGGDYYRGLAPKKG
                     LAFARIIGHKSFVYQEGLEQRARKEVGGNYGLLTWMTPTRSTQSYMLHQGTKFAERFDA
                     NAYIRIADMWAEFDIRDHTPDGTFQTALEGFRRAGIPALIFSIDTDCCFRPAEQQDFAA
                     QLEAAHIPTEFHTIASTKGHDSFLLEPELYAEPIRRILEARKPKGTA"
     CDS             complement(2519..2821)
                     /locus_tag="ctg74_4"
                     /transl_table=11
                     /translation="MLYCIFLIKASLRRRHLTFGKEMTLQFLTKRYISSPEPNTKPELY
                     TMKVLSSLASMKRRHADCQIVKRKGTLYVICKSNPKFKARQGATAGTRLSKKGVK"
     CDS             2971..4896
                     /locus_tag="ctg74_5"
                     /transl_table=11
                     /translation="MTDSSSPDSSPASRNTFAHHLGMKGFLLMEGLLKLVGMKTLYRLG
                     RAAGAVAWHLLPQRRNIVERNLRIVLDPALRGKELQKLSRENFKRTIANFLCSAKTATL
                     TDEQLKHCVTVGGHEQFAAPVLEGRGQVCAIAHSGNWEALARIRAFYPEVERYGSMYRQ
                     FDNPLMEEYVYQRRTERGTQMFSKEGGIKAPMKMLKEGGALGVLSDQFVWEGVYVPFFG
                     KVTGTTPLPALLRKRAGADMVAIAVRTDAPGHWIADMGNVVDFSRSDGSLAGDTIEVNR
                     GLETLIRESVLDVFWMHHRWKSVDRFAPQDKKTAALLENMELKPYRILVAVPRALDEAL
                     ATVPLIRALKTVRCDMQVNIVCPSAQLGIWKTVPEVTHVLPHDSLKQLREALAADEFYN
                     DGPLDLGVMLDGDMETLKALEPYGPMMFSGLDTHPGARKYKFRVKAPVLRAAPPAHRVQ
                     LYLQLGGLHGLDAATPSLFPVKKAAPAEDAPILIAPFSRLGSASEWSWEQWRELVSLLP
                     GRTVLAALEEDRERAASLAEHLNVELAVGAPEALFPVMDGAAAAVAVDGDLPSLCSFRG
                     LPVVTLFSTRLPDVYRPMGTFNRSLYSHQCCSPCFRKECDRDVPCNRHIAVRDVLEALR
                     EISGKE"
     CDS             complement(5145..5924)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 53.7;
                     E-value: 9e-17)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg74_6"
                     /sec_met_domain="Radical_SAM (E-value: 9.6e-32, bitscore:
                     99.6, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MPQVFPQNHDPDGESSVTGLVHSVESCGTVDGPGIRFVLFLSGCS
                     LRCRYCHNPDTSYVRRGRLRSADDVLKEIARYRDFLQAAGGGVTLSGGDPLFQPDFAGA
                     VLKGCRKLGLHTCLDTSGHLGVNAGEEMLADTDLVLLDIKAWNPKRYRALTGGELHPTL
                     EFAERLASLRKPVWLRYVLVPGVTDNLEEIEELSRHAALLGNVERVDVLPFHQLGRFKW
                     DELKLDYTLKDVPEASAELAEQVRGIFRQGGFPDCVH"
     CDS             complement(5941..7128)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg74_7"
                     /sec_met_domain="Gly_radical (E-value: 2.8e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2.4e-121, bitscore:
                     394.9, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="QTLYNLGPAPEPNLTVLWSRNLPEAFKSFCAKVSIETSSVQYEND
                     DLMRPHWGDDYGIACCVSAMRIGKQMQFFGARANLAKCLLYALNGGVDELKGKQVAPPS
                     PRYTEEILNYDEVMALYDKMQDWLAKTYIDALNIIHYMHDKYCYERIEMALHDPEILRT
                     MATGIAGLSVAADSLSAIKYATVKAIRNEEGLIVDFKTEGEFPCYGNNDPRVDDIACSL
                     VSNFMEKLRRLHTYRNSLPTQSILTITSNVVYGKKTGNTPDGRRAGEPFAPGANPMHGR
                     DRNGAVASMLSVAKLSYDDSLDGISYTFSIVPQALGKEERERRVKLVSLLDAYFAATGH
                     HINVNVLERKTLLDAMDHPEKYPQLTIRVSGYAVNFIKLTREQQQEVINRTFHTR"
ORIGIN
        1 cggtacgggg tcttaccagg cccgcggcaa tacgacggga gccggtttcg gcgccatgta
       61 cgagttggcg tatgacatcg ctctcaatga agacaggagc gtgattctcc agcctttgtt
      121 taacgcttcc atcgtgacga cccgcatgga cggctatcgg gaaagcggtt ctgccggaaa
      181 tgccggactg aaggtggaag accaggaact gactacggct gctgtcgctg tgggtgcccg
      241 gctctccgga cttatggggt ccaatatctt cgggcgtgaa gcgctgggag aagtacgggt
      301 gaacgtttcc caggatatgg gagaccgccg cgggcaggct aatgtgggct tcctggcaga
      361 tccctcctat acgcgtccgg tgtatggcgc caaggtcgga agcacggcct tccagattgg
      421 cgcggggctg agcgttcccg tgggcaccca gggcgtcatt tttgtggacg gcaacgcgga
      481 tatccgcagc ggctccagct ccatcaacgg gagtatcggc taccgctaca acttctaaag
      541 gaaaaaacgt cggccaattg gaaagccgct gcggatgatt ccgcagcggc ttttttcatg
      601 aatgggcggg atttctttaa ggataacttc aagcttgggt tgacgtccct gatggctgct
      661 tacttccatt cccattcaat gggcgtttcc agatcaggcc gcagcgcctg gcgcacaggg
      721 caggacaagg cggttttttc cagcatggcg cggagagggt ggctgccttc caggggcatg
      781 attgccttca gttcaatttt ttcaattccc ttggatgatt ccgccgcacg cgccagaacg
      841 cgcatgcctt tcaaatccac tccttttctg gccgcgacca cggaaaccag gctcatcatg
      901 caggaggcga gcgtggaacc gagcaaaact ccgggggagg ggaattctgc gcggccttca
      961 aaagaagcgg gcaggtccgt ggcaaacgta acgttggaag gaataaaggt ggtttcgcag
     1021 cggaattgtc cgacgttctc cgtttcggag gtgtaataat gatcagtcat gaaggtgtga
     1081 cagcgaaaac cctgttcggg ttcatccggt tcaggttatt ttcacgttga agagccgtga
     1141 agttccgctt tgcagggaac ctggccgttc cttcatgaca tggttgattt ttccctcttg
     1201 cttccggttc ccggcatcac ttctttttaa gcgttgctcc gccgtgtctt cttgcggcgg
     1261 gaaaaagctt acgccgtccc cttcggcttc cttgcctcca gaatgcgccg gatgggttcc
     1321 gcataaagtt ccggctccag caggaaggaa tcgtgtccct tggtggaagc gatggtatgg
     1381 aactccgtgg gaatatgggc ggcttcaagc tgcgccgcga aatcctgctg ttccgccggg
     1441 cggaaacagc agtctgtatc aatggaaaag ataagcgcgg gaatccctgc gcggcggaag
     1501 ccttccaggg cagtttgaaa tgttccgtcc ggggtgtggt cgcggatgtc gaactccgcc
     1561 cacatatccg caatacggat ataggcgttg gcgtcaaagc gctccgcgaa cttggttccc
     1621 tggtgaagca tgtagctttg cgtgctgcgg gtgggggtca tccacgtgag caggccgtag
     1681 ttgcctccca cctcttttct ggcgcgctgt tccagcccct cctggtaaac aaatgatttg
     1741 tggccgatga tacgggcgaa cgccagccct tttttgggcg ccagtccccg gtagtaatcc
     1801 ccgccttgga aatccgggtc aagctcaatg gccaggattt gttcaaacag ggacaggcgg
     1861 tgttcgatgg aagcccggta accggagccg atggagatga agctccggac ccgttccggg
     1921 aactggcacg cgaagctgag ggcgatcagg ccgcccacgg aggggcccat aagatgaacg
     1981 cgttctatgc ccaggctgtc cagcagaagg gcctggagac gtgcctggtc tgcggcctcc
     2041 acatgcggga agcgggagcc gtagggctgg ccgtccgcag ggcagggggt agcggggcct
     2101 gtggtgccgt agcagcctcc caggtaattg gcgcagatga tgcagaagcg gtttgtgtcc
     2161 agcggcttgc cggggccgat gatgctgttc caccagccct cgtggttttc cggtttccag
     2221 agggaaccga tgcccgggac ttccggattg taaccgtagg cgtgctggct gccggaaaga
     2281 gcatggaaca gcaggatggc gttgtcttta ttcggcgtca gggttccgta ttgttcgtag
     2341 gcgacgcgaa ccttgtccag cgtagctcca tttctcaggg agaaggagtt gggaagatcc
     2401 aggaatttgg tttggacggc aggaagctgc atggatgtac ggaaaggggc ttggtcagca
     2461 aaaaaagacc ggcacccggc gggcggcggt cttcttgaaa tctgtgctgg gccagggatt
     2521 acttaacgcc ctttttggaa aggcgcgtgc ctgccgtagc gccctggcgg gccttgaact
     2581 tgggattgct cttgcagatc acatacagcg tgcccttgcg cttcacgatt tggcagtctg
     2641 cgtggcggcg cttcatagag gctaaggaag aaagaacttt catggtgtat aactccggtt
     2701 tggtgttcgg ttcgggagac gaaatatacc gtttagttaa gaattgtaaa gtcatttctt
     2761 tgccaaaagt aagatgccgt ctccgcagag atgcttttat gaggaagatg cagtaaagca
     2821 tgggatggct ttggattccg cgccggcgga aaaatttagg gaagacgtca tttagccggg
     2881 aaaataaaat gcgggaaggg atctctgccg ggggaataaa gggtttgtgg accggagggg
     2941 attcgtataa catcttttcc ggttttcctt atgactgatt cttcctctcc tgattcctcc
     3001 ccggcttccc ggaatacgtt tgcccatcat ttgggaatga aagggtttct gctcatggaa
     3061 gggctgttga agctcgtggg catgaaaacg ctgtaccggc tggggcgcgc ggcaggggcc
     3121 gtggcgtggc atctgcttcc ccagcgccgg aacattgtgg aaaggaatct gcgcatcgtt
     3181 ttggatccgg ccctgcgcgg aaaggaactg cagaaattaa gccgggaaaa tttcaaaagg
     3241 accattgcca attttttgtg ttccgcgaag acggccaccc tgacggatga gcagttgaag
     3301 cattgcgtga cggtcggagg gcacgaacag tttgccgcgc ctgtgctgga agggcgcggg
     3361 caggtttgcg ccatagccca ctccggcaac tgggaggcgc tggcgagaat acgcgcattt
     3421 tacccggagg tggagcgtta cgggtccatg taccgccagt ttgacaatcc gctgatggag
     3481 gaatacgtgt accagcggcg cacggaacgt ggaactcaga tgttctccaa ggagggaggc
     3541 atcaaggctc ccatgaaaat gctcaaggaa gggggagccc tgggcgtgct gagcgaccag
     3601 ttcgtatggg agggggtgta tgtccccttt ttcggcaagg tgaccggcac gactccgctg
     3661 cccgctctgc tgagaaaaag ggctggcgcg gacatggtgg ccatagcggt gcgcacggat
     3721 gctcccggcc actggatagc ggatatgggg aatgtggtgg atttttccag gtcggacggc
     3781 tctctggcgg gggacacgat tgaagtgaac agggggctgg aaacattgat ccgggaatcc
     3841 gtgctggatg tgttctggat gcaccatcgc tggaaatccg ttgaccgatt tgccccgcag
     3901 gataaaaaaa cggctgcgct cctggaaaat atggagctga agccgtaccg cattctggta
     3961 gccgttccca gggcgctgga tgaagccctg gcgactgttc ccctgattcg agctttgaaa
     4021 accgtccggt gcgacatgca ggtgaacatc gtctgcccgt ctgcccagct ggggatatgg
     4081 aagacggtgc cggaagtcac ccatgtgctg ccgcacgact ccctgaagca gcttcgggaa
     4141 gctttggcag cagatgaatt ttacaatgac ggaccgctgg atctgggcgt catgctggat
     4201 ggagatatgg aaaccctgaa agcgctggaa ccgtatggcc ccatgatgtt ttccgggctg
     4261 gacacgcatc ccggtgcccg gaagtacaag ttccgggtaa aggcccccgt cctgcgcgcc
     4321 gcccccccgg cgcaccgggt gcagctttat ctgcaattgg gggggctgca tgggctggac
     4381 gccgccaccc cgtctttgtt ccctgtgaaa aaagcggccc cggcggaaga tgcccccatt
     4441 ttaatcgctc cgttttcccg cctggggagc gccagtgaat ggagctggga gcagtggagg
     4501 gagctggttt cccttctgcc gggacggacg gtgctggcgg ctctggaaga agaccgggag
     4561 cgcgccgcgt ccctggcgga acatttgaat gtggagctgg cggtgggtgc gccggaagct
     4621 ctctttcccg tcatggacgg cgctgcggcg gcagtagccg tggatggcga cttgccgtcc
     4681 ctctgttctt tccgggggct tccggtggtg actctgtttt ccacccggct tccggatgtt
     4741 taccgcccta tgggcacatt caaccgttcc ctttacagcc accagtgctg ttctccctgc
     4801 ttccggaagg aatgtgaccg ggatgttccc tgcaaccgcc acattgccgt gcgggacgta
     4861 ctggaagcct tgcgggaaat ttccgggaaa gaataatctg ggaagggacg gaatgccgtc
     4921 ccccggcgtt ggcggaaaaa acggcgggaa tgctgccgtt gccgtaaaga atgatgaaat
     4981 aacgaaagga attgcggaaa tgccgtattc cggagcaatt ccagaaatgc gtcttccttt
     5041 ttgcatggat tctgcagtgg aggggagagg gcccctcatc cggaagaggg ggatgcctgc
     5101 gtccggtttg ctgcggttgg tggcgccaaa gcgcgaaaat ccggttaatg cacgcaatcc
     5161 ggaaagccgc cctgccggaa aatgccgcgg acctgttccg ccagttctgc ggacgcttcc
     5221 gggacgtcct tcaaggtata gtccagcttc aattcatccc atttgaatct ccccagctgg
     5281 tggaaaggga gcacgtccac gcgttccacg ttgcccagaa gcgccgcatg gcgggagagt
     5341 tcctcaattt cttccagatt gtccgtaacg ccaggtacca gcacatacct cagccacacg
     5401 ggcttgcgga gggaggccag gcgttccgca aactccagtg tagggtgcag ttccccgcct
     5461 gtcagggcgc gataccgttt cggattccat gccttgatat ccagcaggac cagatccgta
     5521 tccgccagca tttcttcgcc cgcattcacg cccagatggc cggaggtgtc caggcaggtg
     5581 tgcaggccca gtttccggca gcctttcagc acggcaccgg caaaatccgg ctggaagagg
     5641 gggtccccgc cggagagggt gacgccgccg cccgccgcct gcaaaaaatc ccggtaacgg
     5701 gctatttcct tgaggacatc atctgcgctc ctgagccggc ccctccgcac gtaggaggta
     5761 tccggattat ggcagtagcg gcagcgcagg ctgcaaccgg acaggaaaag gacgaagcgg
     5821 atgccggggc cgtccacggt gccgcaggat tccacggaat gaaccaaacc ggtgacggag
     5881 ctttctccgt ccggatcatg gttttgtgga aaaacctgcg gcatccgctt gcagtccggg
     5941 ttaacgggtg tggaaagtgc ggttgatgac ctcctgctgc tgttcccggg tcagcttgat
     6001 gaaattcacg gcatagccgg aaacgcggat ggtaagctgc gggtattttt ccgggtgatc
     6061 catggcgtcg agaagcgttt tccgttccag tacgttcaca ttaatgtggt gacctgtagc
     6121 ggcaaagtag gcgtccagca gggagacgag cttgacgcgg cgttcacgtt cttccttgcc
     6181 cagggcctga ggaacgatgg agaaggtgta ggagatgccg tccagggagt cgtcatagga
     6241 cagcttggct acggagagca tggaggccac ggctccgttc ctgtcccttc cgtgcatggg
     6301 gttggctccg ggggcgaacg gttccccggc tcggcggccg tccggcgtgt tgcccgtctt
     6361 tttgccgtac accacgttgg aggtgatcgt caggatggac tgggtgggca gggaattgcg
     6421 gtaagtgtgg agcctgcgca gtttttccat gaaattgctg accaggctgc atgctatgtc
     6481 gtccacgcgc gggtcattgt tcccgtaaca ggggaattcc ccttccgtct tgaagtccac
     6541 aatcagccct tcttcattgc ggatggcttt tacggtggca tacttgatgg cggacaggga
     6601 gtccgccgcc acggaaagcc cggcgattcc cgtagccatc gtgcgcagaa tttccggatc
     6661 atgcagcgcc atttcaatgc gttcatagca gtatttgtcg tgcatgtagt ggatgatgtt
     6721 cagcgcgtca atgtaagttt tggcgagcca gtcctgcatc ttgtcgtaca gggccatcac
     6781 ctcatcatag ttgagaattt cctccgtgta gcggggggag ggcggggcca cctgcttgcc
     6841 cttgagttca tccacgccgc cgttcagggc gtagagaagg cacttggcga ggttggcgcg
     6901 cgctccgaag aactgcatct gcttgccgat gcgcatggcg gacacgcagc atgcgatgcc
     6961 gtagtcgtcg ccccaatgcg ggcgcatcag gtcgtcgttc tcgtattgga cggaggacgt
     7021 ttcaatggaa accttggcgc agaagctttt gaaggcttcc ggcaggttcc gggaccacag
     7081 aacggtcagg ttcggttccg gagccggccc caggttgtac agggtctgc
//
