LOCUS       c00163_gnlPro..         4566 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|PAHHILEL_163.
ACCESSION   c00163_gnlPro..
VERSION     c00163_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 15:30:15
            Original ID  :: gnl|Prokka|PAHHILEL_163
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4566
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4566
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[149:4566]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      150..4566
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..4566
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4566
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             150..620
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg163_1"
                     /sec_met_domain="Glyoxalase_4 (E-value: 3.6e-26, bitscore:
                     80.1, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYCRPPPCIPYNHHTHMIQQIDHIGIAVKSLDATVPYYRDALGLG
                     EPHIEEVPTQKVRVAMFDVAGVHIELLEPTSPESAIAKAIEKKGEGIHHIAFKTNDIAG
                     NISQAKEAGLTVLNDPPVPGAHNTQVTFLHPKCTFGVLTEFCQHPGGCECGK"
     CDS             652..2217
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg163_2"
                     /sec_met_domain="CT_Vparvula (E-value: 4.6e-217, bitscore:
                     709.8, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAIDPKLIDDLNSRRKKVILSGGQEKIDKRHEKGEMTARDRMGYL
                     FEEGTFSEIGMHVRHNCHNFGMGKKEIPGDGVVSGFGLVDGRPVACAASDFLAQGGSLG
                     YMHAMKIADAQKYALKAGIPMVTVNDSGGARLQEGVAALSGYAQVFYNNVLASGVVPQI
                     SMILGPCAGGAAYSPALTDFIIMRNSGNAGMYITGPKVIEQVTYEKCTMDDIGSAAIHA
                     TVSGNVHFVADSDAHAMDILKRLLSFLPSNNTEEPPHKLDTPLDLSADEGMSDLIPGDN
                     RTPLDVQPIISRLVDNGDFLEVHKDFAKNVVVGFGRICGVVVGIIANQPNVKAGCLDID
                     SSDKAARFIRFCNAFNIPLVNLVDVPGFLPGKNQERGGIIRHGAKLIFAYSQATVPKVT
                     LIMRKAYGGAYIAMCCKDLGADAVFAWPGAEIAVMGAEGAVPVLYGRELKAVEDPAEKA
                     KRQGELLEEYREAFYNPYVAAGMGQITEVINPEETRAKIAFALRTLLNKKEVRPAKKHG
                     NIPL"
     CDS             2239..2691
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg163_3"
                     /sec_met_domain="OAD_gamma (E-value: 2.1e-10, bitscore:
                     29.7, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLTTLAFAFAQGIANVMNNLNYQVVGIMVVMLCLGGMAVILTVS
                     GSIAAAIQNRAKAKTAAPVAVSAAAPAAAGKPGMTPEMLAILSAAVDSAMTELTPEMVA
                     VISAAVDAGLDGMSHRIVEIKQSPGSGYAAAGRAEIFASHRIRPSR"
     CDS             2764..3165
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 58.8; E-value:
                     1.2e-18)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg163_4"
                     /sec_met_domain="Biotin_lipoyl (E-value: 9.9e-20, bitscore:
                     58.8, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKLRITVDGKVFDVSVELLDQVSSTTAAPAPAPVAAPAPAASAP
                     VAAPAPAPAPAPAPAAAGAGDVPSPLAGKVVSLDVAAGAPVKAGDQILTLEAMKMNTII
                     YAPASGTLTAFCVNPGDTVQEGQALAKIG"
     CDS             3283..4566
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg163_5"
                     /sec_met_domain="OAD_beta (E-value: 1.6e-105, bitscore:
                     341.2, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGVFSLSWQMVGMWGIAILLLYLGVAKQYEPLLMVPIAFGALIAN
                     IPDNGMLITQLNQQVISSNEQGEVTATSLNNVGFLRVHVAPLQQAPSKVPANLTTPESR
                     AQYQEIMQQPMQVYPGSQLTVSKIKSVRESQEKAKADAARLGDDSLTVDPNLKDFQNVT
                     EDNGNEPVFLLTNGEGTTVVRQQGVNYFDTSGNRIPVDLKTQKLEPLVVSAAGKYVAVG
                     QHTQELLVTSIHGGLYDWIGLGIKAEIFPPIIFLGVGALTDFGPLLAAPRTLLLGAAAQ
                     VGVAATFFMALFMGFTPNESASIGIIGGADGPTSIFLTMKLAPHLLGAVAVAAYTYMSL
                     VPLIQPPIMALLTTKKERLIRMKSLRTVSKSEKLFFAVLVTIVTILLIPDASPLIGMLM
                     LGNFLRECKVTERLVQASQNAIINIVTIF"
ORIGIN
        1 agaaataaca cggctaatcc actcttgaac atgcccctac catacctcat cttccggggc
       61 aatcaagcag gtgaaagggg cggccacgaa ttcgccattt ttccgctttt cgcttcacat
      121 cctaaaaaaa tacgctttaa taactcttga tgtattgcag gcctcccccc tgcattccct
      181 ataaccatca cacgcatatg attcagcaaa ttgaccatat cggcatcgcc gtcaagagtc
      241 ttgacgccac cgtcccctat taccgcgatg cgctcgggct cggagagccc cacattgagg
      301 aagttcccac tcaaaaggtc cgcgtcgcca tgtttgacgt tgccggagtc cacatcgaac
      361 tgctcgaacc cacctctccg gaaagcgcta tcgccaaggc tattgagaaa aaaggggaag
      421 gcatccacca catcgctttc aagaccaatg acatcgccgg aaatatctcc caggccaagg
      481 aagcaggcct taccgtgctg aacgacccgc ccgtccccgg cgctcacaac acgcaggtca
      541 ccttcctgca tcccaaatgc accttcggcg tcctgaccga attctgccag caccccggcg
      601 gctgcgaatg cggcaagtaa cattctccct attcaccata ccactagacc aatggctatt
      661 gatcccaaat tgattgacga tctgaacagc cgccgcaaga aagtcatcct cagcggcggt
      721 caggaaaaga tcgacaagag acatgaaaag ggcgaaatga cagcccgcga ccgcatgggg
      781 tacctctttg aggaaggcac cttctcggaa atcggcatgc atgtgcgcca caactgccac
      841 aacttcggca tgggtaagaa ggaaatcccc ggcgacggcg tcgtttccgg tttcggcctg
      901 gtggacggcc gcccggtagc ctgcgcggcc tccgatttcc tggcccaggg cggttccctc
      961 ggctacatgc acgccatgaa aattgcggat gcccagaagt atgcgctgaa agccggcatc
     1021 ccgatggtga ccgtgaacga ctccggcggc gcgcgcctcc aggaaggagt ggcggccctt
     1081 tccggatacg cccaggtgtt ttacaacaac gtgcttgcct ccggcgtggt tccgcaaatc
     1141 tccatgatcc tgggcccctg cgcgggcggc gcggcctact cccccgccct gacggacttc
     1201 atcatcatgc gcaattctgg caacgcgggc atgtacatca ccggtcccaa agtgattgaa
     1261 caggtcacgt atgaaaaatg cacgatggac gacatcggct ccgccgccat tcacgccacc
     1321 gtgtccggca acgtccattt cgtagcggac agcgacgcgc acgccatgga catcctgaag
     1381 aggcttcttt ccttccttcc ttccaacaac acggaagaac cgccccacaa actggacact
     1441 ccgctggacc tgagcgctga cgaaggcatg agcgacctga ttcccggcga caaccgcacg
     1501 ccgctggacg tccagcccat catcagccgt ctggtggaca acggggactt cctggaagta
     1561 cacaaggact tcgccaaaaa tgtcgtcgtc ggattcggcc gcatctgcgg cgtggtggtc
     1621 ggcatcatcg ccaaccagcc caatgtgaaa gccggctgcc tggatatcga ctcctccgac
     1681 aaggccgcgc gcttcatccg tttctgcaac gcgttcaata ttccgttggt gaacctggtg
     1741 gacgtgcccg gcttcctgcc cggcaagaac caggaacggg gcggcattat ccgccacggc
     1801 gccaaactta tcttcgccta ttcccaggcc acggtgccca aagtcaccct gatcatgcgg
     1861 aaagcctacg gcggcgccta catcgccatg tgctgcaagg acctgggcgc tgacgccgtc
     1921 ttcgcctggc ctggtgcgga aattgccgtt atgggtgcgg aaggcgccgt cccggtactc
     1981 tacggccgcg aactgaaggc tgtggaagac ccggcggaaa aagccaagcg ccagggagaa
     2041 cttctggaag aataccggga agccttttac aacccgtatg tggcggccgg catgggacag
     2101 attacggaag tcatcaatcc ggaagaaacc cgcgccaaaa tcgccttcgc cctgcgcacc
     2161 ctgctgaaca agaaggaagt gcgcccggcc aagaaacacg gcaacattcc gctctaacat
     2221 ccatcccaac ggattcatat gttacttaca acactcgctt ttgccttcgc acagggcatc
     2281 gccaacgtta tgaacaatct gaactatcag gttgtcggca tcatggtggt tatgctgtgc
     2341 ctgggaggca tggccgtcat tctgaccgtc agcggctcca tcgccgccgc cattcagaac
     2401 agggccaaag ccaaaacagc cgctcccgta gccgtgtctg ccgccgctcc cgctgcggcg
     2461 ggcaaaccgg gcatgactcc ggaaatgctc gccatccttt ccgccgccgt ggattccgcc
     2521 atgacggagc ttacgccgga aatggtggcc gtcatctccg ccgccgtgga cgcaggcctg
     2581 gacggcatga gccaccgcat cgtggaaatc aagcaatccc cgggttccgg ctatgcggct
     2641 gcgggcaggg cggaaatttt cgcctcccac cgcatccggc cttcccgctg aactactgca
     2701 ttattcaccc ttaccccgcc caggcggaat catttaactt aatatcccat accacacact
     2761 attatgaaga aacttcgcat caccgtagac ggcaaagtat ttgacgtatc cgtagaactt
     2821 ctggaccagg tgtcctccac cactgcggcg ccggcccccg cacctgtggc cgctcccgct
     2881 cctgcggctt ccgcccccgt ggccgccccg gccccggctc ccgcacctgc gccggctccg
     2941 gccgccgccg gcgcaggaga cgttcccagc ccgcttgccg ggaaggtcgt ctccctggac
     3001 gtggcagcag gcgcacccgt gaaggccggt gaccagattc tgacgctgga agccatgaag
     3061 atgaacacca tcatctacgc tcccgcctcc ggcacgctca ccgctttctg cgtgaacccg
     3121 ggcgacacgg ttcaggaagg acaggcactg gccaaaattg gttaatcccg gcggcggcag
     3181 cccggaaacg ccaacagtcc cgggctccgg ctcagataat aaaccgctac aaaaatattc
     3241 cctatgagtc tttttgattc attaatcacc tttttacagg ggatgggcgt cttctcccta
     3301 tcatggcaga tggtagggat gtggggcatt gccattctcc tcctgtatct gggtgtagcc
     3361 aagcaatacg aaccgctcct gatggttccc attgctttcg gcgccctcat tgccaacatt
     3421 ccggataacg gaatgctcat cacccagctg aaccagcagg tcatctcctc caatgaacag
     3481 ggggaagtaa ccgccacgtc tctgaacaac gtggggttcc tgcgcgttca cgtggctccg
     3541 cttcagcagg ctccctccaa ggtgccggcc aacctgacca cgcctgaatc ccgtgcccag
     3601 tatcaggaaa tcatgcagca gcccatgcag gtttaccccg gcagccagct gaccgtttcc
     3661 aagatcaagt ccgtgcggga atcccaggaa aaagcaaaag ctgacgcggc ccgcctgggc
     3721 gacgacagcc tgacggtgga ccccaacctg aaggatttcc agaacgtgac ggaagacaac
     3781 ggcaatgaac cggtcttcct gctcacgaac ggagaaggga ccaccgtcgt ccgccagcag
     3841 ggcgtcaatt actttgacac cagcggcaac cggattccgg tggatctgaa aacccagaag
     3901 ctggagcccc tggtggtttc cgccgcaggc aaatacgtgg ccgtaggcca gcatacccag
     3961 gaactgctgg tcacctccat ccacgggggc ctgtatgact ggatcgggct gggcatcaag
     4021 gctgaaattt tcccgcccat catctttctg ggcgtaggag ccctgacgga cttcggcccg
     4081 ctgcttgccg ctccgcgcac cctgcttctg ggcgcagcgg cccaggtggg cgtcgccgcc
     4141 acgttcttca tggccctgtt catggggttc accccgaatg aatccgcgtc catcggcatc
     4201 attggcggcg ctgacggccc cacctccatc ttcttgacga tgaagctggc cccgcacctg
     4261 ctgggcgccg tggccgtggc cgcatacacg tacatgtccc tggttccgct gatacagccg
     4321 cccatcatgg cgttgctgac gaccaagaag gaacgcctca tccgcatgaa atccctgcga
     4381 acggtcagca agagcgaaaa actgtttttc gccgtgctgg taaccattgt caccattttg
     4441 ctgattccgg acgcatcccc gctgatcggc atgctgatgc ttggcaactt cctgcgcgaa
     4501 tgcaaggtga cggaacgcct ggttcaggcc tcccagaatg caatcatcaa catcgtcacc
     4561 atattc
//
