LOCUS       c00169_gnlPro..         5948 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|EFFDIHEF_169.
ACCESSION   c00169_gnlPro..
VERSION     c00169_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 19:42:26
            Original ID  :: gnl|Prokka|EFFDIHEF_169
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 5948
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..5948
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[109:3085]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      110..3085
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..5948
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..5948
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(110..844)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 67.6;
                     E-value: 4.7e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg169_1"
                     /sec_met_domain="Radical_SAM (E-value: 5.1e-34, bitscore:
                     106.2, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLRYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             complement(845..3085)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg169_2"
                     /sec_met_domain="Gly_radical (E-value: 1.6e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 2.3e-282, bitscore:
                     925.8, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRRVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             complement(3256..5559)
                     /locus_tag="ctg169_3"
                     /transl_table=11
                     /translation="MEQCEENSQENDDVIIEKEISKLIEEQRLFTESIYACLPVGIEIY
                     DAHGVLRSINDHALRMYGVDDRTSVVNIVNLYKSPFVDSELLARIQSGEDISLEFEYDF
                     DRVNKDAYYSSYNKDTMIYGVKVIPIWSRKKHLIGHILLANDMTSVKEVEFRTEESKKN
                     LEMAMKAANMASWVYDVNKKVFNPLYGLTVAKKNMPMEAVLDILHPQDRAPLIRLFSQL
                     IGNEIEQGQMTLRFYNEQEEMYRYYESRMRLSTEHRGKLQIIGTQMDITERLQMAKKAQ
                     DLIAKRDLAMKVSNIVHWDFDVNSQKFESYNDPINDYVSDRLLTVSEYMDVIYPEDRSV
                     FYDAMQSLIAGKNVTINFTCRIQTKYDETWQYCDFMGVPFDQDENGDIIRYTGFRQNIP
                     KIQQLNRELKERNYKIELSFKTVGMSYWDFDVKSLKFSAFNDPINDFYSEKTITPEDYL
                     NATHPDDVVFVRKYMEHMFRGIDKDFNVKYRSKTKWDDEWQTLLVTGIQVERDKEGHVT
                     RYTGITINNTKWEKMIQQLKELKEKAELSDRLKSAFLANMSHEIRTPLNAIVGFSELMV
                     TCDDPEEKKEYINIIQSNNELLLRLINDILDLSKIESGIIERRKENFNLAKVCNELFVT
                     IQAKMTNPNVELRLDGPNSECWVLLDRNRLKQVWMNFLTNAVKCTKSGYIKMGYGIERK
                     GLRIYVEDTGIGIPDDLHDKVFTRFEKLNEFSQGTGLGLTISRAIVEAAGGEVGFTSKL
                     GVGSTFWAWLPLRP"
     CDS             5716..5946
                     /locus_tag="ctg169_4"
                     /transl_table=11
                     /translation="MRFTRLILALTSFLILITQTDLFASTVLHKTSMVDLSNNAIICMH
                     QDPDGYLWIGTYDGLNLYNGKDTYVYRFELNN"
ORIGIN
        1 tagtcaaggt tagtctaggc aaaaaattat tactatcgtt taaaagctaa tcggattaga
       61 ttgttttccc tgattagctg ttgcataatc gcaaggcttg atcgagagat caggccttgt
      121 tattttctgg tttgtagcga gagaatatcg ctttcgcatt atctaaacgt tcttttgata
      181 agggttccac cccctttaac ggataatcga gacctaattg ctcatattta taggttccca
      241 tcgtatgata aggcaataat tccactttgc gaaccacttt atatgaactg acataccgag
      301 ctaacgcctc cagccactcg tcattatccg tatatccggg gacaataaca tggcgaatcc
      361 aagtatctat gccacgtctc tccaattcat ccaagaacag aagcgtatta tcctgcttga
      421 cacccgccag taacggatgt aaatccgggt ttaaggtttt gatgtccaat aacactaagt
      481 ccgcataatc caagacttcc caagcttttg aggtacatac gaaaccagat gtgtccaaag
      541 ctgtgtgaag accttgctct tggcacaaac ggaaaaactc tttcagaaac tcaggttgca
      601 ataaaggttc cccaccggta acagtcaccc ccccacgggc gataaaactc ttataccgta
      661 agacttccgt caatagctcg ccgggagtca tctgatattt caccttccct ttcgggttcc
      721 aagtatccgg gttatggcaa tacaggcaac gcaacgggca gccctgcata aaaacgacaa
      781 agcgaattcc gggaccatct acggtcccga aactctctaa tgaatgtatc ttacctttta
      841 tcacttacag tgaatggtta atggttcttg aaataacatc caactgttgt tcacgagtca
      901 gtttcacgaa gtttaccgca tatccggata cacggatcgt aagttgcgga tatttctcag
      961 gatgttccat agcgtcgatc aacaaatcct tatcaaatac gttaacattc agatgctgac
     1021 ctccatccgg agtaaaatag ccatccaata agctaaccag attattgatc tgcacttcac
     1081 gctcctttcc caacgtagcc ggagaaacag cgaaagtata agagataccg tcgtgcgcat
     1141 gctggaatgg aagcttcgct accgaagcta gcgcagcgat agcacctttc gtatcacgac
     1201 cgttcatagg gttcgcgccc ggagcgaaag gagttccacc gatacggcca tccggtgtcg
     1261 taccagtctt cttaccatat accacattag aggtgatcgt aaggatggac tgcgtaggtt
     1321 tcgcatggcg ataggtctcg tgctgacgca aatattccat gaacttctcg gtgatctcta
     1381 cagcgatact atccgtacga tcgtcgttat taccgaaagg aacatattcg ccctcacgtt
     1441 cgaaatcaac cgccaagcca cgctcgtcac ggatcacacg tactttcgta tcacggatag
     1501 cagccaacga atcggcaacg atagaaagac cggcgatacc tgtcgcacgg atacgctcta
     1561 catctccatc gtgaagcgac atctcgaatg cctcatatgc gtatttatca tgcatataat
     1621 ggattatctt caaggcgttc acatacactt tcgccaacca gcgcatcatt tgctcatact
     1681 tagccatcac ctcatcgtat tccagatact cgccgcgaac cggctcgaac atcggggcta
     1741 cctgtacgcc gctgcgctca tcccgtcctc cgttgatcgc atataataaa catttcgcca
     1801 aattcgcacg ggctccgaag aactgcatct gcttaccgat cttcatagga gaaacgcaac
     1861 aagcgatacc ataatcatct ccataatccg gacgcatcaa atcgtcgttc tcatattgga
     1921 tagccgatgt atcgatagag accttggcac agaaacgttt ccagttctcg ggagcgttct
     1981 taaaccacag aaccgtcaaa ttgggttctg gtgccggacc tagattatat aatgtatgga
     2041 gataacgata ggaagttctt gttaccaaag aacggccatc cactccttgg ccgcccaagg
     2101 actctgttac ccaaaccgga tcaccggaga acaaatcatt gtattccggc gtacgtaaga
     2161 aacgaacgat acgcaacttg atgatcagct ggtctaccaa ttcttgcgcc tcctcttcgg
     2221 tcaacttacc ctcacggatg tctttctcga tataaatatc caaaaacgta gaagtacgtc
     2281 cgatagacat cgccgcgcca tcttggtctt ttaccgccgc cagataagct aagtacacaa
     2341 actgaactgc ctcacgagcg ttttgtgccg gacgagtcac atcaaagcca tagctagcgc
     2401 acattttctc gaacgctttc aaagccttga tctgttcgga aatctcctca cggctctgga
     2461 tgatctcttc cgtgatctct tggatatcca aacgattcaa gaaacgcttt ttctcctcga
     2521 tcaagattgc cgtaccatat aaagcgacac gacgatagtc accaataata cgtcctctac
     2581 catacgcgtc gggcagaccg gtgataatag ccgaacgacg tgcggcaatc atctcttccg
     2641 tatatgcgga gaaaacgcct tcgttatgag ttttacggta cttcgtgaag atctctttcg
     2701 tcatcgggtc caaatgataa ccataagctt ccaagctatt ctcaaccata cggataccgc
     2761 ccttcgggaa gataccacgt ttcagcggag catcggtttg taacccaacg atcacctcat
     2821 tctccttatc aatatagccc gggccatata catcgatccc ttgaggtaat tttgtctcgg
     2881 cgtcataaac tcccttttcc ctctcgacct taaacatctc ggtcagcttg ttccagaccc
     2941 tacgggtctt ctcggaagaa gcgactaaaa aggaatcatc cccttcataa ggcgtatagt
     3001 tactctggat aaaatctctt acattgatct ctcttttcca ggtctcacct ttaaagtgat
     3061 cccagcactc attctcaaat ttcataaaca atcttatcta ataccacatt tttaccgccg
     3121 caaaggtagg agaattatcg tcatatttct gtcacatatg ctacaccagt tctaaaatcc
     3181 ctataaattg cgataaaaaa gcttctcatc cttcactatt ttaaaaggac attaccaata
     3241 aatggggagt cagacctaag ggcgaagagg caaccatgcc caaaaagtag atccgacacc
     3301 aagttttgaa gtaaagccca cttctccccc tgccgcttct acgatagctc tggagatcgt
     3361 caagccaagt ccggtaccct gcgagaactc attcaatttc tcaaaacggg taaatacttt
     3421 gtcatgcaaa tcatccggta taccgattcc cgtatcctca acatagatac gaagaccttt
     3481 cctttcaatg ccatatccca ttttaatata tccggatttg gtgcatttta ccgcattagt
     3541 gaggaaattc atccatactt gcttaagccg gtttctatcc agaagtaccc agcactccga
     3601 gttgggacca tctaaacgta actcgacatt cggatttgtc atcttcgcct gaatcgtaac
     3661 aaataactca ttacaaacct tagccagatt gaaattctcc ttcctacgtt caattatgcc
     3721 tgactctatc ttggaaagat caaggatatc gttgatcaat cgcaataaca actcattatt
     3781 ggattggatg atatttatat attctttttt ttcttccgga tcatcacaag ttaccattaa
     3841 ctctgaaaaa ccaacaatag cgtttaacgg ggtacgtatc tcgtgactca tattagcgag
     3901 aaacgcagac ttgagacgat ccgaaagttc cgctttctcc tttaattcct tcaattgctg
     3961 aatcatcttc tcccacttcg tattattgat cgtaataccg gtatagcgag tcacatggcc
     4021 ttctttatct ctttcaacct ggattccagt aaccaataag gtctgccact cgtcatccca
     4081 tttggttttg gaacgatatt taacgttgaa atctttatct atgccccgga acatatgctc
     4141 catatatttc cgcacaaaaa ccacatcatc cggatgtgta gcgttcaaat aatcctcggg
     4201 agtgattgtc ttttccgaat aaaaatcatt aataggatca ttaaaagcac tgaatttcag
     4261 gctttttaca tcaaaatccc aatacgacat accaaccgtc ttgaaagaaa gctctatctt
     4321 gtaatttctt tctttaagtt cccgattcaa ttgctggatc ttaggaatgt tttgcctgaa
     4381 tcccgtatag cgtattatat ctccattttc atcttgatcg aaagggacac ccataaaatc
     4441 acaatactgc caagtctcat catacttggt ttgtatgcga caggtaaaat tgatggtgac
     4501 attctttcca gctatcaggg attgcattgc gtcgtaaaaa acagaccggt cctcggggta
     4561 tataacatcc atatactccg aaacggtcaa taatcgatct gaaacgtaat cgttgatcgg
     4621 atcattataa gattcgaatt tctgggaatt tacatcaaaa tcccaatgaa caatattact
     4681 gactttcatg gccagatcac gttttgcgat cagatcctgc gctttcttgg ccatttgcaa
     4741 cctttcggtt atatccattt gtgtcccgat gatctgcaat tttccacgat gttctgtcga
     4801 taggcgcatc cggctctcat aataacgata catctcttct tgctcgttat agaaacgcaa
     4861 agtcatttgt ccttgttcta tctcatttcc tatcaattga gagaagagcc ggattaacgg
     4921 agctcgatct tggggatgca aaatatccag taccgcctcc attggcatat tttttttggc
     4981 tacggttagt ccatataatg gattaaaaac ctttttattc acatcatata cccaagaagc
     5041 catattagcc gctttcatgg ccatctccag atttttcttg ctttcttccg tacggaattc
     5101 cacctctttc acggaagtca tatcatttgc gagtaatata tgtccgataa gatgtttttt
     5161 tctactccat atcgggataa ctttaacccc atagatcatc gtgtctttat tataggaaga
     5221 ataatatgca tccttattaa cccgatcaaa atcatactca aactccaagg aaatatcttc
     5281 acccgattgt atccgagcta aaagttctga atcaacaaat ggacttttgt aaagattgac
     5341 tatattcacc accgatgtcc ggtcatccac accatacatc ctcaacgcat ggtcgtttat
     5401 actacgcagc acaccatgtg cgtcataaat ttcaataccc accggcagac aagcatatat
     5461 actctccgta aataaacgct gttcttctat taatttagaa atttctttct ctattatcac
     5521 atcgtcattt tcttgagaat tctcttcaca ttgttccatc tgtcacttac tttatttcgt
     5581 attatcatac gtcagacatt ctaatttata cgaaagccct gctttttgca aaagtaaaca
     5641 aaaaagtgaa ttcttttctg ttcgttcata aagatattat ctttgcgctg aaaacaaaca
     5701 ctaaatctta aatacatgag attcacaagg cttattctag cgttaaccag tttccttata
     5761 ttgatcacgc aaacagacct atttgcctca accgtattgc ataaaacttc tatggttgat
     5821 ctctcaaata acgctattat ttgcatgcat caagatccgg atggctattt gtggatcggg
     5881 acttacgacg gacttaactt atacaacgga aaagatacgt atgtatatcg tttcgaatta
     5941 aataacaa
//
