LOCUS       c00004_gnlPro..         6454 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FEKMLOKM_4.
ACCESSION   c00004_gnlPro..
VERSION     c00004_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 13:38:16
            Original ID  :: gnl|Prokka|FEKMLOKM_4
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6454
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..726)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: AcetylCoA_hydro"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg4_1"
                     /sec_met_domain="AcetylCoA_hydro (E-value: 4e-45, bitscore:
                     143.3, seeds: 5, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSYKVLTPEEAASLIKHGHHIGLSGFTPAGTAKAVTAALAKIAEA
                     EHEKGNPFQVSVFTGASTGDSCDGILSRAKAIRYRAPYTTNVDFRKAVNNGEIAYNDIH
                     LSQMAQEVRYGFMGNVDIAIIEACEVTADGKIYLTAAGGIAPTICRLADKIIVELNAAH
                     SKSAMGLHDVYEPLDPPYRREISIFKPSDRIGKPYIQVDPKKIVGVVETNWPDEARSFA
                     AADPLTDKIGQNVADFLAAD"
     protocluster    1..6454
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:6373]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..6373
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..6454
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6454
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(788..1567)
                     /locus_tag="ctg4_2"
                     /transl_table=11
                     /translation="MNSAASFSTPPEEEGPCGDWTYHEWAEAFSTNDLARALPPGHIAV
                     CRVQTGGRGRFNRKWIGEEGGLWASFTVPLSAPEDTAAGVHWGHLPLVAGLALFNMLRG
                     MGLDSVRLRWPNDLLAGKSKLAGILVERPAENMAVIGIGINVFNDVSSIAGEIKDPPAR
                     LADLVRDCPSLHAVMASLGSHLNQVLSVFSHGGVPSLQEGLSRAWAGERPVSILTDDET
                     VRGIFTGIDDQGNPVLSLPAGTIRTIPAHLINRLLEE"
     CDS             complement(1605..2840)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg4_3"
                     /sec_met_domain="OAD_beta (E-value: 3.2e-147, bitscore:
                     479.1, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VRESQEKAKADAARLGDDSLTVDPNLKDFQNVTEDNGNEPVFLLT
                     NGEGTTVVRQQGVNYFDTSGNRIPVDLKTQKLEPLVVSAAGKYVAVGQHTQELLVTSIH
                     GGLYDWIGLGIKAEIFPPIIFLGVGALTDFGPLLAAPRTLLLGAAAQVGVAATFFMALF
                     MGFTPNESASIGIIGGADGPTSIFLTMKLAPHLLGAVAVAAYTYMSLVPLIQPPIMALL
                     TTKKERLIRMKSLRTVSKSEKLFFAVLVTIVTILLIPDASPLIGMLMLGNFLRECKVTE
                     RLVQASQNEIINIVTIFLGTSVGLTMQGDRFLQSETLLIILLGIVAFGVATAGGVIAAK
                     IMNLIWRKNPVNPLIGSAGVSAVPMAARVSHNVGQKYDPSNYLLMHAMGPNVAGVIGTA
                     VIAGYYIATLAK"
     CDS             complement(2809..3240)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg4_4"
                     /sec_met_domain="OAD_beta (E-value: 6.3e-17, bitscore:
                     50.6, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MGVFSLSWQMVGMWGIAILLLYLGVAKQYEPLLMVPIAFGALIAN
                     IPDNGMLITQLNQQVISSNEQGEVTATPLNNVGYLRVHVAPLQQAPSKVPANLTTPESR
                     AQYQEIMQQPMQVYPXXXXQPADRFQDQVRAGIPGKSQG"
     CDS             complement(3358..3759)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 58.8; E-value:
                     1.2e-18)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg4_5"
                     /sec_met_domain="Biotin_lipoyl (E-value: 1.6e-19, bitscore:
                     58.8, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKLRITVDGKVFDVSVELLDQVSSTTAAPAPAPVAAPAPAASAP
                     VAAPAPAPAPAPAPAAAGAGDVPSPLAGKVVSLDVAAGAPVKAGDQILTLEAMKMNTII
                     YAPASGTLTAFCVNPGDTVQEGQALAKIG"
     CDS             complement(3832..4284)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg4_6"
                     /sec_met_domain="OAD_gamma (E-value: 1.8e-10, bitscore:
                     30.5, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLTTLAFAFAQGIANVMNNLNYQVVGIMVVMLCLGGMAVILTVS
                     GSIAAAIQNRAKAKTAAPVAVPAAAPAAAGKPGMTPEMLAILSAAVDSAMTELTPEMVA
                     VISAAVDAGLDGMSHRIVEIKQSPGSGYAAAGRAEIFASHRIRPSR"
     CDS             complement(4306..5871)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg4_7"
                     /sec_met_domain="CT_Vparvula (E-value: 7.3e-217, bitscore:
                     709.8, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAIDPKLIDDLNSRRKKVILSGGQEKIDKRHEKGEMTARDRMGYL
                     FEEGTFSEIGMHVRHNCHNFGMGKKEIPGDGVVSGFGLVDGRPVACAASDFLAQGGSLG
                     YMHAMKIADAQKYALKAGIPMVTVNDSGGARLQEGVAALSGYAQVFYNNVLASGVVPQI
                     SMILGPCAGGAAYSPALTDFIIMRNSGNAGMYITGPKVIEQVTYEKCTMDDIGSAAIHA
                     TVSGNVHFVADSDAHAMDILKRLLSFLPSNNTEEPPHKLDTPLDLSADEGMSDLIPGDN
                     RTPLDVQPIISRLVDNGDFLEVHKDFAKNVVVGFGRICGVVVGIIANQPNVKAGCLDID
                     SSDKAARFIRFCNAFNIPLVNLVDVPGFLPGKNQERGGIIRHGAKLIFAYSQATVPKVT
                     LIMRKAYGGAYIAMCCKDLGADAVFAWPGAEIAVMGAEGAVPVLYGRELKAVEDPAEKA
                     KRQGELLEEYREAFYNPYVAAGMGQITEVINPEETRAKIAFALRTLLNKKEVRPAKKHG
                     NIPL"
     CDS             complement(5903..6373)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg4_8"
                     /sec_met_domain="Glyoxalase_4 (E-value: 5.8e-26, bitscore:
                     80.1, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYCRPPPCIPYNHHTHMIQQIDHIGIAVKSLDATVPYYRDALGLG
                     EPHIEEVPTQKVRVAMFDVAGVHIELLEPTSPESAIAKAIEKKGEGIHHIAFKTNDIAG
                     NISQAKEAGLTVLNDPPVPGAHNTQVTFLHPKCTFGVLTEFCQHPGGCECGK"
ORIGIN
        1 gtcggcagcc aggaaatcgg ccacgttttg cccgatttta tcggtcaggg gatccgctgc
       61 ggcgaaggaa cgggcttcat ccggccagtt ggtttccaca acgcccacaa tctttttcgg
      121 gtcaacctgg atgtacggct tgccgatgcg gtcgctgggt ttgaagatgg aaatttcgcg
      181 gcggtaggga ggatccaggg gttcgtaaac gtcgtgcagg cccatggcgc tcttgctgtg
      241 ggcggcgttt aattccacga taattttgtc tgccaggcgg cagatggtgg gagcgatccc
      301 gcccgcggcc gtcaggtaaa ttttgccgtc ggccgtgact tcacaggctt caataatggc
      361 gatgtccacg ttgcccatga acccgtagcg cacttcctgg gccatctggg acaaatggat
      421 gtcattatag gcgatttccc cgttgttcac cgccttgcgg aaatcaacgt tggtggtgta
      481 gggagcgcgg tagcggattg ccttggcccg ggaaaggatc ccgtcgcagg aatcgccggt
      541 ggaagcaccg gtgaagacgc tcacctggaa agggttcccc ttctcatgtt ccgcttctgc
      601 gatttttgcc agcgcagccg taacggcctt ggccgttccg gcgggcgtga atccgctcag
      661 cccaatatgg tggccatgct tgatcaggct tgctgcttct tctggtgtca gtaccttgta
      721 actcatgtca gtgatttttg ttggatgaaa aaagaaagaa accccgtgta aaaacggagc
      781 ggtaggctca ttcttcgagg agcctgttga tcaggtgcgc cggaatggtg cggatggtgc
      841 cggccggaag ggatagcaca ggattgccct gatcgtcaat ccctgtaaaa atgccccgga
      901 cggtttcgtc atccgtaaga atggaaaccg gcctttctcc ggcccatgcc cgggacagcc
      961 cctcctgaag ggaggggacg ccgccgtggg aaaagacgga aagaacctgg ttcaaatgac
     1021 tgcccaggga agccatgacg gcgtgcaggg aagggcagtc ccgaacaaga tccgccagcc
     1081 ttgccggagg gtccttgatt tccccggcga tggaggatac atcattgaaa acattgatgc
     1141 cgatgccgat gacggccatg ttctccgccg ggcgttccac cagtattccc gccagtttgg
     1201 atttgcccgc cagcaggtcg ttcggccagc gcaggcgcac ggaatccagc cccatgccgc
     1261 ggagcatgtt gaacagggcc agccccgcaa ccaggggcaa atgtccccag tgcacgccgg
     1321 ccgctgtatc ctcaggagcg gacaggggga cggtgaagga tgcccacagg ccgccttcct
     1381 cccctatcca tttgcggttg aacctgccgc ggcctcctgt ctgaacgcgg cagacggcaa
     1441 tatgccctgg ggggagggcg cgggccagat cattggtgga aaaagcctcg gcccattcat
     1501 gataagtcca gtcgccgcat ggtccttcct cctccggagg ggtggaaaat gaggctgcgg
     1561 aattcatggg acgggccgag gccgggagta attttcaatt ctggttattt cgccagagtg
     1621 gcgatgtagt agcccgcgat gactgcggtg ccgatcacgc ctgcaacgtt cggacccatg
     1681 gcgtgcatga gcaggtagtt ggaggggtcg tacttctggc ccacattgtg ggagacgcgg
     1741 gctgccatcg ggacggcgga gacgcctgcg gacccgatca gggggttgac cggattcttg
     1801 cgccagatga ggttcataat cttggcggca atcactccgc ccgctgttgc cacgccgaag
     1861 gcgacgatac ccagaaggat gatcagcagc gtttccgact gcaggaagcg gtcgccctgc
     1921 atcgtgagcc ccacggaggt tcccaggaag atggtgacga tgttgatgat ttcattctgg
     1981 gaggcctgaa ccaggcgttc cgtcaccttg cattcgcgca ggaagttgcc aagcatcagc
     2041 atgccgatca gcggggatgc gtccggaatc agcaaaatgg tgacaatggt taccagcacg
     2101 gcgaaaaata gtttttcgct cttgctgacc gttcgcaggg atttcatgcg gatcaggcgt
     2161 tccttcttgg tcgtcagcaa cgccatgatg ggcggctgta tcagcggaac cagggacatg
     2221 tacgtgtatg cggccacggc cacggcgccc agcaggtgcg gggccagctt catcgtcagg
     2281 aagatggagg tggggccgtc agcgccgcca atgatgccga tggacgcgga ttcattcggg
     2341 gtgaacccca tgaacagggc catgaagaac gtggcggcta cgcccacctg ggccgctgcg
     2401 cccagaagca gggtgcgcgg agcggcaagc agcgggccga agtccgtcag ggctcctacg
     2461 cccagaaaga tgatgggcgg gaaaatttca gccttgatgc ccagcccgat ccagtcatac
     2521 aggcccccgt ggatggaggt gaccagcagt tcctgggtat gctggcctac ggccacgtat
     2581 ttgcctgcgg ctgaaaccac caggggctcc agcttctggg ttttcagatc caccggaatc
     2641 cggttgccgc tggtgtcaaa gtaattgacg ccctgctggc ggacgacggt ggtcccttct
     2701 ccgttcgtga gcaggaagac cggttcattg ccgttgtcct ccgtcacgtt ctgaaaatct
     2761 ttcaggttgg ggtccaccgt caggctgtcg tcgcccaggc gggccgcgtc agccttggct
     2821 ttttcctggg attcccgcac ggacttgatc ttggaaacgg tcagctggct gccnnnnnnn
     2881 nnnggggtaa acctgcatgg gctgctgcat gatttcctga tactgggcac gggattcagg
     2941 cgtggtcaga ttggccggca ccttggaggg agcctgctga agcggagcca cgtgaacgcg
     3001 caggtacccc acgttgttca ggggcgtagc ggttacttcc ccctgttcat tggaggagat
     3061 gacctgctgg ttcagctggg tgatgagcat tccgttatcc ggaatgttgg caatgagggc
     3121 gccgaaagca atgggaacca tcaggagcgg ttcgtattgc ttggctacac ccagatacag
     3181 gaggagaatg gcaatgcccc acatccctac catctgccat gatagggaga agacgcccat
     3241 cccctgtaaa aaggtgatta atgaatcaaa aagactcata gggaatattt ttgtagcggt
     3301 ttattatctg agccggagcc cgggactgtt ggcgtttccg ggctgccgcc gccgggatta
     3361 accaattttg gccagtgcct gtccttcctg aaccgtgtcg cccgggttca cgcagaaagc
     3421 ggtgagcgtg ccggaggcgg gagcgtagat gatggtgttc atcttcatgg cttccagcgt
     3481 cagaatctgg tcaccggcct tcacgggtgc gcctgctgcc acgtccaggg agacgacctt
     3541 cccggcaagc gggctgggaa cgtctcctgc gccggcggcg gccggagccg gcgcaggtgc
     3601 gggagccggg gccggggcgg ccacgggggc ggaagccgca ggagcgggag cggccacagg
     3661 tgcgggggcc ggtgccgcag tggtggagga cacctggtcc agaagttcta cggatacgtc
     3721 aaatactttg ccgtctacgg tgatgcgaag tttcttcata atagtgtgtg gtatgggata
     3781 ttaagttaaa tggttccgcc tgggcggggt aagggtgaat aatgcagtag ttcagcggga
     3841 aggccggatg cggtgggagg cgaaaatttc cgccctgccc gcggccgcat agccggaacc
     3901 cggggattgc ttgatttcca cgatgcggtg gctcatgccg tccaggcctg cgtccacggc
     3961 ggcggagatg acggccacca tttccggcgt aagctccgtc atggcggaat ccacggcggc
     4021 ggaaaggatg gcgagcattt ccggagtcat gcccggtttg cccgccgcag cgggagcggc
     4081 ggcaggcacg gctacgggag cagcggtttt ggctttggcc ctgttctgaa tggcggcggc
     4141 gatggagccg ctgacggtca gaatgacggc catgcctccc aggcacagca taaccaccat
     4201 gatgccgaca acctgatagt tcagattgtt cataacgttg gcgatgccct gtgcgaaggc
     4261 aaaagcgagt gttgtaagta acatatgaat ccgttgggat ggatgttaga gcggaatgtt
     4321 gccgtgtttc ttggccgggc gcacttcctt cttgttcagc agggtgcgca gggcgaaggc
     4381 gattttggcg cgggtttctt ccggattgat gacttccgta atctgtccca tgccggccgc
     4441 cacatacggg ttgtaaaagg cttcccggta ttcttccaga agttctccct ggcgcttggc
     4501 tttttccgcc gggtcttcca cagccttcag ttcgcggccg tagagtaccg ggacggcgcc
     4561 ttccgcaccc ataacggcaa tttccgcacc aggccaggcg aagacggcgt cagcgcccag
     4621 gtccttgcag cacatggcga tgtaggcgcc gccgtaggct ttccgcatga tcagggtgac
     4681 tttgggcacc gtggcctggg aataggcgaa gataagtttg gcgccgtggc ggataatgcc
     4741 gccccgttcc tggttcttgc cgggcaggaa gccgggtacg tccaccaggt tcaccaacgg
     4801 aatattgaac gcgttgcaga aacggatgaa gcgcgcggcc ttgtcggagg agtcgatatc
     4861 caggcagccg gctttcacat tgggctggtt ggcgatgatg ccgaccacca cgccgcagat
     4921 gcggccgaat ccgacgacga catttttggc gaagtccttg tgtacttcca ggaagtcccc
     4981 gttgtccacc agacggctga tgatgggctg gacgtccagc ggcgtgcggt tgtcgccggg
     5041 aatcaggtcg ctcatgcctt cgtcagcgct caggtccagc ggagtgtcca gtttgtgggg
     5101 cggttcttcc gtgttgttgg aaggaaggaa ggaaagaagc ctcttcagga tgtccatggc
     5161 gtgcgcgtcg ctgtccgcta cgaaatggac gttgccggac acggtggcgt gaatggcggc
     5221 ggagccgatg tcgtccatcg tgcatttttc atacgtgacc tgttcaatca ctttgggacc
     5281 ggtgatgtac atgcccgcgt tgccggaatt gcgcatgatg atgaagtccg tcagggcggg
     5341 ggagtaggcc gcgccgcccg cgcaggggcc caggatcatg gagatttgcg gaaccacgcc
     5401 ggaggcaagc acgttgttgt aaaacacctg ggcgtatccg gaaagggccg ccactccttc
     5461 ctggaggcgc gcgccgccgg agtcgttcac ggtcaccatc gggatgccgg ctttcagcgc
     5521 atacttctgg gcatccgcaa ttttcatggc gtgcatgtag ccgagggaac cgccttgggc
     5581 caggaaatcg gaggccgcgc aggctaccgg gcggccgtcc accaggccga aaccggaaac
     5641 gacgccgtcg ccggggattt ccttcttacc catgccgaag ttgtggcagt tgtggcgcac
     5701 atgcatgccg atttccgaga aggtgccttc ctcaaagagg taccccatgc ggtcgcgggc
     5761 tgtcatttcg cccttttcat gtctcttgtc gatcttttcc tgaccgccgc tgaggatgac
     5821 cttcttgcgg cggctgttca gatcgtcaat caatttggga tcaatagcca ttggtctagt
     5881 ggtatggtga atagggagaa tgttacttgc cgcattcgca gccgccgggg tgctggcaga
     5941 attcggtcag gacgccgaag gtgcacttgg gatgcaggaa ggtgacctgc gtgttgtgag
     6001 cgccggggac gggcgggtcg ttcagcacgg taaggcctgc ttccttggcc tgggagatat
     6061 ttccggcgat gtcattggtc ttgaaagcga tgtggtggat gccttcccct tttttctcaa
     6121 tagccttggc gatagcgctt tccggagagg tgggttcgag cagttcgatg tggactccgg
     6181 caacgtcaaa catggcgacg cggacctttt gagtgggaac ttcctcaatg tggggctctc
     6241 cgagcccgag cgcatcgcgg taatagggga cggtggcgtc aagactcttg acggcgatgc
     6301 cgatatggtc aatttgctga atcatatgcg tgtgatggtt atagggaatg caggggggag
     6361 gcctgcaata catcaagagt tattaaagcg tattttttta ggatgtgaag cgaaaagcgg
     6421 aaaaatggcg aattcgtggc cgcccctttc acct
//
