LOCUS       c00225_gnlPro..         2368 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|FEKMLOKM_225.
ACCESSION   c00225_gnlPro..
VERSION     c00225_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 13:38:16
            Original ID  :: gnl|Prokka|FEKMLOKM_225
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 2368
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..2368
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[1:2168]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      2..2168
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..2368
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..2368
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             2..1486
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 96.1; E-value:
                     8.4e-30)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg225_1"
                     /sec_met_domain="FAD_binding_2 (E-value: 4.1e-31, bitscore:
                     96.1, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 3.5e-39,
                     bitscore: 121.5, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="QLKRTFYARGQTGQQLLLGAYQALEKEVAKGHIEMFSRHEMMDLV
                     LVDGEAKGIVTRNLVTGEIKSYAGDTVLLCTGGYGNVFFLSTNAMGCNVTAAYKAYKRG
                     AFFANPCFTQIHPTCIPVKGDYQSKLTLMSESLRNSGRIWVPKSRETAEKIRKKEIKPS
                     DVPEEERDYYLERKYPSFGNLAPRDISSRAAKEACDDGRGVAVTGKGVFLDFADEIKRM
                     GREWIDGQYGNLFDMYEEITDDDPHETPMMIYPASHYTMGGLWVDYNLMSNVKGLHVLG
                     EANFSDHGANRLGASALMQGLSDGYFVIPATLPAYLTTTKPGAVTTAAPEFKAAEDEVK
                     ERINKLLNVKGTKTVDSFHRELGLIMWEDCGMARTEESLTHAIERIPQLRKEFWENVRV
                     TGTADGINAELEKACRVADFLEFAQLLCYDALNRQESCGAHFRVEYQLPDGEAKRNDDE
                     YAYVAAWEYTGVDSLPVLHKEPLTFENVHLAIRSYK"
     CDS             1527..2168
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Fer4_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg225_2"
                     /sec_met_domain="Fer2_3 (E-value: 6.7e-25, bitscore: 75.1,
                     seeds: 38, tool: rule-based-clusters)"
                     /sec_met_domain="Fer4_4 (E-value: 1.6e-07, bitscore: 19.5,
                     seeds: 79, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAKTLNLTLKVWRQENREAKGRIETYAAKDIPVDASFLEMLDIVN
                     EGLVKEGKEPIHFDHDCREGICGMCSLTINGIPHGPERQVTTCQLHMRKFKDGDTVWIE
                     PFRAKAFPILRDLMVDRSALDRIIAAGGYIDVRTGSAPNANNIAVEKVKADAAFDAAAC
                     IGCGACVASCKNASAMLFTSAKIAHLNLLPQGQPERSKRVLAXXXXXXXP"
     CDS             2165..2317
                     /locus_tag="ctg225_3"
                     /transl_table=11
                     /translation="MMKQMEAEGFGNCTNQYECEAACPKGISVDNIARLNRDFMRANAK
                     EGLCF"
ORIGIN
        1 ccagctcaag cgaaccttct acgcacgcgg acagacgggc cagcagctac tgctgggtgc
       61 ctaccaggcc ctggaaaagg aagtggccaa gggacacatt gaaatgttct cccgccatga
      121 aatgatggat ctggtgctgg tggacggcga agccaagggc atcgtcaccc gcaatctggt
      181 taccggcgaa atcaaatcct acgcagggga taccgtcctg ctctgcacgg gcggctacgg
      241 caacgtgttc ttcctgtcca ccaacgcgat gggttgcaac gtcactgccg cctacaaggc
      301 ctacaagcgc ggtgccttct tcgccaaccc ctgcttcacg caaatccacc ccacctgcat
      361 tccggtcaag ggcgactatc agtccaaact gacgctgatg tcggaatccc tccgcaacag
      421 tggccgcatc tgggtgccca agtcccgtga aaccgcagaa aaaatccgta aaaaggaaat
      481 caagccttcc gatgttcccg aagaagaacg ggactactac ctggaacgca agtatccctc
      541 cttcggcaac ctggctccgc gcgacatttc ctcccgcgcc gccaaggaag cctgcgacga
      601 cggccgcggc gtggccgtca ccggcaaggg cgtcttccta gattttgcgg atgaaatcaa
      661 gcgcatgggc cgcgaatgga ttgacggcca atacggcaat ctgtttgaca tgtacgaaga
      721 aatcacggac gacgaccccc atgaaacgcc catgatgatc taccccgcct cccactacac
      781 catgggtggc ctgtgggtgg actacaacct catgtccaat gtgaaggggc tgcacgtcct
      841 cggtgaagcc aacttctccg accacggcgc caaccgcctg ggagcctccg ccctgatgca
      901 gggcctgtct gacggctact tcgtcattcc cgccacgctg cccgcttacc tgacgaccac
      961 caaacccggc gccgtcacca ccgccgcccc ggaattcaag gctgcggaag acgaagtgaa
     1021 ggaacgcatc aacaagctcc tgaatgtgaa gggcaccaag acggtggact ccttccaccg
     1081 ggaactgggc ctcattatgt gggaagactg cggcatggcc cgcacggaag aaagcctgac
     1141 ccacgccatt gaacgcattc cccagctccg caaggaattc tgggaaaacg tgcgcgtaac
     1201 cggcacggcg gacggcatca acgcggaact ggaaaaagcc tgccgcgtgg ccgacttcct
     1261 ggaattcgcc cagctgctct gctatgacgc cctgaaccgc caggaatcct gcggcgccca
     1321 cttccgcgtg gaataccagc tgccggacgg cgaagctaag cgcaacgacg atgaatacgc
     1381 ctatgtagcc gcctgggaat acactggcgt ggacagcctg cctgttctcc acaaggaacc
     1441 gctgaccttc gagaacgtgc accttgccat ccgcagctac aagtaaagat agccaataca
     1501 ttaacactca aaagaaagat tataacatgg ctaaaacact caatctcact ctcaaggtct
     1561 ggcgccagga aaaccgggaa gccaagggcc gcattgagac ctatgccgcc aaggacattc
     1621 cggtggacgc ctccttcctg gaaatgctgg acatcgtcaa tgaaggcctg gtcaaggaag
     1681 gcaaggaacc catccatttt gaccacgact gccgggaagg catctgcggc atgtgctccc
     1741 tgaccatcaa cggcattccc cacgggccgg aacgccaggt gaccacttgc cagcttcaca
     1801 tgcgcaagtt caaggatggc gataccgtct ggattgaacc cttccgcgcg aaggctttcc
     1861 caatcctgcg ggacctgatg gtggaccgct ccgccctgga ccgcatcatc gccgctggag
     1921 gctatattga cgtgcgtacc ggttccgcgc cgaacgccaa caacatcgcc gtggaaaaag
     1981 tgaaagcgga cgccgccttt gacgcggccg cctgcatcgg ctgcggcgcc tgcgtggcct
     2041 cctgtaaaaa cgcctccgcc atgctgttca cctccgccaa gatcgcccat ctcaatctgc
     2101 tgccgcaggg ccagccggaa cgttccaagc gcgtcctggc cannnnnnnn nnnnnnnnnn
     2161 ggccatgatg aagcagatgg aagcggaagg ctttggcaac tgcacgaacc agtatgaatg
     2221 tgaagccgcc tgcccgaaag gcatcagcgt ggacaacatc gcccgcctca accgcgactt
     2281 catgcgggcc aacgccaagg aaggcctctg tttctaaaga agattccttc ttaagacgct
     2341 ctgtaaaaac tcccggcggc atcggctg
//
