LOCUS       c00052_gnlPro..         4401 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NCBMFGBL_52.
ACCESSION   c00052_gnlPro..
VERSION     c00052_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 15:50:21
            Original ID  :: gnl|Prokka|NCBMFGBL_52
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4401
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4401
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:4050]"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..4050
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /neighbourhood="10000"
                     /product="succinate2propionate"
                     /protocluster_number="1"
     cand_cluster    1..4401
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(minimum(4, [AcetylCoA_hyd_C,
                     AcetylCoA_hydro, B12-binding, Biotin_lipoyl, Glyoxalase_4,
                     HMGL-like, MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma,
                     PYC_OADA]) and minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /kind="single"
                     /product="succinate2propionate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4401
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="succinate2propionate"
                     /region_number="1"
                     /rules="(minimum(4, [AcetylCoA_hyd_C, AcetylCoA_hydro,
                     B12-binding, Biotin_lipoyl, Glyoxalase_4, HMGL-like,
                     MM_CoA_mutase, MeaB, OAD_beta, OAD_gamma, PYC_OADA]) and
                     minimum(1, [CT_Btheta, CT_Propionyl_CoA1,
                     CT_Propionyl_CoA2, CT_Propionyl_CoA3, CT_Vparvula]) and not
                     Cytidylate_kin)"
                     /tool="gutsmash"
     CDS             complement(3..956)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_beta"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg52_1"
                     /sec_met_domain="OAD_beta (E-value: 5.8e-42, bitscore:
                     132.5, seeds: 229, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MSLFDSLITFLQGMGVFSLSWQMVGMWGIAILLLYLGVAKQYEPL
                     LMVPIAFGALIANIPDNGMLITQLNQQVISSNEQGEVTATSLNNVGYLRVHVAPLQQAP
                     SKVPANLTTPESRAQYQEIMQQPMQVYPGSQLTVSKIKSVRESQEKAKADAARLGDDSL
                     TVDPNLKDFQNVTEDNGNEPVFLLTNGEGTTVVRQQGVNYFDTSGNRIPVDLKTQKLEP
                     LVVSAAGKYVAVGQHTQELLVTSIHGGLYDWIGLGIKAEIFPPIIFLGVGALTDFGPLL
                     AAPRTLLLGAAAQVGVAATFFMALFMGFTPNESASIG"
     CDS             complement(1035..1436)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Biotin_lipoyl"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1024:Biotin-requiring enzyme (Score: 58.8; E-value:
                     1.2e-18)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg52_2"
                     /sec_met_domain="Biotin_lipoyl (E-value: 1.2e-19, bitscore:
                     58.8, seeds: 40, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKKLRITVDGKVFDVSVELLDQVSSTTAAPAPAPVAAPAPAASAP
                     VAAPAPAPAPAPAPAAAGAGDVPSPLAGKVVSLDVAAGAPVKAGDQILTLEAMKMNTII
                     YAPASGTLTAFCVNPGDTVQEGQALAKIG"
     CDS             complement(1509..1961)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: OAD_gamma"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg52_3"
                     /sec_met_domain="OAD_gamma (E-value: 1.4e-10, bitscore:
                     30.5, seeds: 234, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLLTTLAFAFAQGIANVMNNLNYQVVGIMVVMLCLGGMAVILTVS
                     GSIAAAIQNRAKAKTAAPVAVPAAAPAAAGKPGMTPEMLAILSAAVDSAMTELTPEMVA
                     VISAAVDAGLDGMSHRIVEIKQSPGSGYAAAGRAEIFASHRIRPSR"
     CDS             complement(1983..3548)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: CT_Vparvula"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg52_4"
                     /sec_met_domain="CT_Vparvula (E-value: 5.5e-217, bitscore:
                     709.8, seeds: 132, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MAIDPKLIDDLNSRRKKVILSGGQEKIDKRHEKGEMTARDRMGYL
                     FEEGTFSEIGMHVRHNCHNFGMGKKEIPGDGVVSGFGLVDGRPVACAASDFLAQGGSLG
                     YMHAMKIADAQKYALKAGIPMVTVNDSGGARLQEGVAALSGYAQVFYNNVLASGVVPQI
                     SMILGPCAGGAAYSPALTDFIIMRNSGNAGMYITGPKVIEQVTYEKCTMDDIGSAAIHA
                     TVSGNVHFVADSDAHAMDILKRLLSFLPSNNTEEPPHKLDTPLDLSADEGMSDLIPGDN
                     RTPLDVQPIISRLVDNGDFLEVHKDFAKNVVVGFGRICGVVVGIIANQPNVKAGCLDID
                     SSDKAARFIRFCNAFNIPLVNLVDVPGFLPGKNQERGGIIRHGAKLIFAYSQATVPKVT
                     LIMRKAYGGAYIAMCCKDLGADAVFAWPGAEIAVMGAEGAVPVLYGRELKAVEDPAEKA
                     KRQGELLEEYREAFYNPYVAAGMGQITEVINPEETRAKIAFALRTLLNKKEVRPAKKHG
                     NIPL"
     CDS             complement(3580..4050)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     succinate2propionate: Glyoxalase_4"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg52_5"
                     /sec_met_domain="Glyoxalase_4 (E-value: 4.3e-26, bitscore:
                     80.1, seeds: 51, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MYCRPPPCIPYNHHTHMIQQIDHIGIAVKSLDATVPYYRDALGLG
                     EPHIEEVPTQKVRVAMFDVAGVHIELLEPTSPESAIAKAIEKKGEGIHHIAFKTNDIAG
                     NISQAKEAGLTVLNDPPVPGAHNTQVTFLHPKCTFGVLTEFCQHPGSCECGK"
     CDS             4168..4401
                     /locus_tag="ctg52_6"
                     /transl_table=11
                     /translation="MFKSGLAVLFLLASWFSAACAYELLPAHVAVVYNGKSELSRRMAR
                     EYARVRGVPEGNLVSLDCPMTSEISRKEYEDTI"
ORIGIN
        1 atgccgatgg acgcggattc attcggggtg aaccccatga acagggccat gaagaacgtg
       61 gcggctacgc ccacctgggc cgctgcgccc agaagcaggg tgcgcggagc ggcaagcagc
      121 gggccgaagt ccgtcagggc tcctacgccc agaaagatga tgggcgggaa aatttcagcc
      181 ttgatgccca gcccgatcca gtcatacagg cccccgtgga tggaggtgac cagcagttcc
      241 tgggtatgct ggcctacggc cacgtatttg cctgcggctg aaaccaccag gggctccagc
      301 ttctgggttt tcagatccac cggaatccgg ttgccgctgg tgtcaaagta attgacgccc
      361 tgctggcgga cgacggtggt cccttctccg ttcgtgagca ggaagaccgg ttcattgccg
      421 ttgtcttccg tcacgttctg gaaatccttc aggttggggt ccaccgtcag gctgtcgtcg
      481 cccaggcggg ccgcgtcagc ttttgctttt tcctgggatt cccgcacgga cttgatcttg
      541 gaaacggtca gctggctgcc ggggtaaacc tgcatgggct gctgcatgat ttcctgatac
      601 tgggcacggg attcaggcgt ggtcagattg gccggcacct tggagggagc ctgctgaagc
      661 ggagccacgt gaacgcgcag gtaccccacg ttgttcagag acgtggcggt tacttccccc
      721 tgttcattgg aggagatgac ctgctggttc agctgggtga tgagcattcc gttatccgga
      781 atgttggcaa tgagggcgcc gaaagcaatg ggaaccatca ggagcggttc gtattgcttg
      841 gctacaccca gatacaggag gagaatggca atgccccaca tccctaccat ctgccatgat
      901 agggagaaga cgcccatccc ctgtaaaaag gtgattaatg aatcaaaaag actcataggg
      961 aatatttttg tagcggttta ttatttgagt cggagcccgg tactgttggc gtttccgggc
     1021 tgccgccgcc gggattaacc aattttggcc agtgcctgtc cttcctgaac cgtgtcgccc
     1081 gggttcacgc agaaagcggt gagcgtgccg gaggcgggag cgtagatgat ggtgttcatc
     1141 ttcatggctt ccagcgtcag aatctggtca ccggccttca cgggtgcgcc tgctgccacg
     1201 tccagggaga cgaccttccc ggcaagcggg ctgggaacgt ctcctgcgcc ggcggcggcc
     1261 ggagccggcg caggtgcggg agccggggcc ggggcggcca cgggggcgga agccgcagga
     1321 gcgggagcgg ccacaggtgc gggggccggc gccgcagtgg tggaggacac ctggtccaga
     1381 agttctacgg atacgtcaaa tactttgccg tctacggtga tgcgaagttt cttcataata
     1441 gtgtgtggta tgggatatta agttaaatga ttccgcctgg gcggggtaag ggtgaataat
     1501 gcagtagttc agcgggaagg cctgatgcgg tgggaggcga aaatttccgc cctgcccgcg
     1561 gccgcatagc cggaacccgg ggattgcttg atttccacga tgcggtggct catgccgtcc
     1621 aggcctgcgt ccacggcggc ggagatgacg gccaccattt ccggcgtaag ctccgtcatg
     1681 gcggaatcca cggcggcgga aaggatggcg agcatttccg gagtcatgcc cggtttgccc
     1741 gccgcagcgg gagcggcggc aggaacggct acgggagcgg ctgttttggc tttggccctg
     1801 ttctgaatgg cggcggcgat ggagccgctg acggtcagaa tgacggccat gcctcccagg
     1861 cacagcataa ccaccatgat gccgacaacc tgatagttca gattgttcat aacgttggcg
     1921 atgccctgtg cgaaggcaaa agcgagtgtt gtaagtaaca tatgaatccg ttgggatgga
     1981 tgttagagcg gaatgttgcc gtgtttcttg gccgggcgca cttccttctt gttcagcagg
     2041 gtgcgcaggg cgaaggcgat tttggcgcgg gtttcttccg gattgatgac ttccgtaatc
     2101 tgccccatgc cggccgccac atacgggttg taaaaggctt cccggtattc ttccagaagt
     2161 tcgccctggc gcttggcttt ttccgccggg tcttccacag ccttcagttc gcggccgtag
     2221 agtaccggga cggcgccttc cgcacccata acggcaattt ccgcaccagg ccaggcgaag
     2281 acggcgtcag cgcccaggtc cttgcagcac atggcgatgt aggcgccgcc gtaggctttc
     2341 cgcatgatca gggtgacttt gggcaccgtg gcctgggaat aggcgaagat aagtttggcg
     2401 ccgtggcgga taatgccgcc ccgttcctgg ttcttgccgg gcaggaagcc gggcacgtcc
     2461 accaggttca ccaacggaat attgaacgcg ttgcagaaac ggatgaagcg tgcggccttg
     2521 tcggaggagt cgatatccag gcagccggct ttcacattgg gctggttggc gatgatgccg
     2581 accaccacgc cgcagatgcg gccgaatccg acgacgacat ttttggcgaa gtccttgtgt
     2641 acttccagga agtccccgtt gtccaccaga cggctgatga tgggctggac gtccagcggc
     2701 gtgcggttgt cgccgggaat caggtcgctc atgccttcgt cagcgctcag gtccagcgga
     2761 gtgtccagtt tgtggggcgg ttcttccgtg ttgttggaag gaaggaagga aagaagcctc
     2821 ttcaggatgt ccatggcgtg cgcgtcgctg tccgctacga aatggacgtt gccggacacg
     2881 gtggcgtgaa tggcggcgga gccgatgtcg tccatcgtgc atttttcata cgtgacctgt
     2941 tcaatcactt tgggaccggt gatgtacatg cccgcgttgc cggaattgcg catgatgatg
     3001 aagtccgtca gggcggggga gtaggccgcg ccgcccgcgc aggggcccag gatcatggag
     3061 atttgcggaa ccacgccgga ggcaagcaca ttgttgtaaa atacctgggc gtatccggaa
     3121 agggccgcca ctccttcctg gaggcgcgcg ccgccggagt cgttcacggt caccatcggg
     3181 atgccggctt tcagcgcata cttctgagca tccgcaattt tcatggcgtg catgtagccg
     3241 agggaaccgc cctgggccag gaaatcggag gccgcgcagg ctaccgggcg gccgtccacc
     3301 aggccgaaac cggaaacgac gccgtcgccg gggatttcct tcttacccat gccgaagttg
     3361 tggcagttgt ggcgcacatg catgccgatt tccgagaagg tgccttcctc aaagaggtac
     3421 cccatgcggt cgcgggctgt catttcgccc ttttcatgtc tcttgtcgat cttttcctga
     3481 ccgccgctga ggatgacctt cttgcggcgg ctgttcagat cgtcaatcaa tttgggatca
     3541 atagccattg gtctagtggt atggtgaata gggagaatgt tacttgccgc attcgcagct
     3601 gccggggtgc tggcagaatt cggtcaggac gccgaaggtg cacttgggat gcaggaaggt
     3661 gacctgcgtg ttgtgagcgc cggggacggg cgggtcgttc agcacggtaa ggcctgcttc
     3721 cttggcctgg gagatatttc cggcgatgtc attggtcttg aaagcgatgt ggtggatgcc
     3781 ttcccctttt ttctcaatag ccttggcgat agcgctttcc ggagaggtgg gttcgagcag
     3841 ttcgatgtgg actccggcaa cgtcaaacat ggcgacgcgg accttttgag tgggaacttc
     3901 ctcaatgtgg ggctctccga gcccgagcgc atcgcggtaa taggggacgg tggcgtcaag
     3961 gctcttgacg gcgatgccga tatggtcaat ttgctgaatc atatgcgtgt gatggttata
     4021 gggaatgcag gggggaggcc tgcaatacat caagagttat taaagcgtat ttttttagga
     4081 tgtgaagcga aaagcggaaa aatggcgaat tcgtggccgt ccctttcacc tgcttgattg
     4141 ccccggaaga tgaggtatgg taggggcatg ttcaagagtg gattagccgt gttatttctg
     4201 ctggcgtcgt ggttttccgc cgcgtgtgcc tatgagctgc tgcccgctca tgtggccgtg
     4261 gtttataacg gaaaatccga gctgagccgc cgcatggcca gggagtatgc gcgggtgcgc
     4321 ggcgttccgg aagggaatct cgtttccctg gattgcccca tgaccagcga gatttcgcgg
     4381 aaggaatatg aggatacgat c
//
