LOCUS       c00134_gnlPro..         3377 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|BBHMIDKK_134.
ACCESSION   c00134_gnlPro..
VERSION     c00134_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 14:05:00
            Original ID  :: gnl|Prokka|BBHMIDKK_134
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 3377
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..3377
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[98:3074]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      99..3074
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..3377
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..3377
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             complement(99..833)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 68.1;
                     E-value: 3.3e-21)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg134_1"
                     /sec_met_domain="Radical_SAM (E-value: 5.3e-34, bitscore:
                     105.7, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="VIKGKIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWNPK
                     GKVKYQMTPGELLTEVLQYKSFIARGGVTVTGGEPLLQPEFLKEFFRLCQEQGLHTALD
                     TSGFVCTSKAWEVLDYADLVLLDIKTLNPDLHPLLAGVKQDNTLLFLDELERRGIDTWI
                     RHVIVPGYTDNDEWLEALARYVSSYKVVRKVELLPYHTMGTYKYEQLGLDYPLKGVEPL
                     SKERLDNAKAIFSRYKPENNKA"
     CDS             complement(834..3074)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg134_2"
                     /sec_met_domain="Gly_radical (E-value: 1.2e-46, bitscore:
                     145.5, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 9.2e-283, bitscore:
                     926.7, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MKFENECWDHFKGETWKREINVRDFIQSNYTPYEGDDSFLVASSE
                     KTRKVWNKLTEMFKVEREKGVYDAETKLPQGIDVYGPGYIDKENEVIVGLQTDAPLKRG
                     IFPKGGIRMVENSLEAYGYHLDPMTKEIFTKYRKTHNEGVFSAYTEEMIAARRSAIITG
                     LPDAYGRGRIIGDYRRVALYGTAILIEEKKRFLNRLDIQEITEEIIQSREEISEQIKAL
                     KAFEKMCASYGFDVTRPAQNAREAVQFVYLAYLAAVKDQDGAAMSIGRTSTFLDIYIEK
                     DIREGKLTEEEAQELVDQLIIKLRIVRFLRTPEYNDLFSGDPVWVTESLGGQGVDGRSL
                     VTRTSYRYLHTLYNLGPAPEPNLTVLWFKNAPENWKRFCAKVSIDTSAIQYENDDLMRP
                     DYGDDYGIACCVSPMKIGKQMQFFGARANLAKCLLYAINGGRDERSGVQVAPMFEPVRG
                     EYLEYDEVMAKYEQMMRWLAKVYVNALKIIHYMHDKYAYEAFEMSLHDGDVERIRATGI
                     AGLSIVADSLAAIRDTKVRVIRDERGLAVDFEREGEYVPFGNNDDRTDSIAVEITEKFM
                     EYLRQHETYRHAKPTQSILTITSNVVYGKKTGTTPDGRIGGTPFAPGANPMNGRDTKGA
                     IAALASVAKLPFQHAHDGISYTFAVSPATLGKEREVQINNLVSLLDGYFTPDGGQHLNV
                     NVFDKDLLIDAMEHPEKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINHSL"
     CDS             complement(3245..3376)
                     /locus_tag="ctg134_3"
                     /transl_table=11
                     /translation="FSQGTGLGLTISRAIVEAAGGEVGFTSKLGVGSTFWAWLPLRP"
ORIGIN
        1 agtctaggca aaaaattatt actatcgttt aaaagctaat cggattagat tgttttccct
       61 gattagctgt tgcataatcg caaggcttga tcgagagatc aggccttgtt attttctggt
      121 ttgtagcgag agaatatcgc tttcgcatta tctaaacgtt cttttgataa gggttccacc
      181 ccctttaacg gataatcgag gcctaattgc tcatatttat aggttcccat cgtatgataa
      241 ggcaataatt ccactttgcg aaccacttta tatgaactga cataccgagc taacgcctcc
      301 agccactcgt cattatccgt atatccgggg acaataacat ggcgaatcca agtatctatg
      361 ccacgtctct ccaattcatc caagaacaga agcgtattat cctgcttgac acccgccagt
      421 aacggatgta aatccgggtt taaggttttg atgtccaata acactaagtc cgcataatcc
      481 aagacttccc aagcttttga ggtacatacg aaaccagatg tgtccaaagc tgtgtgaaga
      541 ccttgctctt ggcacaaacg gaaaaactct ttcagaaact caggttgcaa taaaggttcc
      601 ccaccggtaa cagtcacccc cccacgggcg ataaaactct tatactgtaa gacttccgtc
      661 aatagctcgc cgggagtcat ctgatatttc accttccctt tcgggttcca agtatccggg
      721 ttatggcaat acaggcaacg caacgggcag ccctgcataa aaacgacaaa gcgaattccg
      781 ggaccatcta cggtcccgaa actctctaat gaatgtatct taccttttat cacttacagt
      841 gaatggttaa tggttcttga aataacatcc aactgttgtt cacgagtcag tttcacgaag
      901 tttaccgcat atccggatac acggatcgta agttgcggat atttctcagg atgttccata
      961 gcgtcgatca acaaatcctt atcaaatacg ttaacattca gatgctgacc tccatccgga
     1021 gtaaaatagc catccaataa gctaaccaga ttattgatct gcacttcacg ctcctttccc
     1081 aacgtagccg gagaaacagc gaaagtataa gagataccgt cgtgcgcatg ctggaatgga
     1141 agcttcgcta ccgaagctag cgcagcgata gcacctttcg tatcacgacc gttcataggg
     1201 ttcgcgcccg gagcgaaagg agttccaccg atacggccat ccggtgtcgt accagtcttc
     1261 ttaccatata ccacattaga ggtgatcgta aggatggact gcgtaggttt cgcatggcga
     1321 taggtctcgt gctgacgcaa atattccatg aacttctcgg tgatctctac agcgatacta
     1381 tccgtacgat catcgttatt accgaaagga acatattcgc cctcacgttc gaaatcaacc
     1441 gccaagccac gctcgtcacg gatcacacgt actttcgtat cacggatagc agccaacgaa
     1501 tcggcaacga tagaaagacc ggcgatacct gtcgcacgga tacgctctac atccccatcg
     1561 tgaagcgaca tctcgaatgc ctcatacgcg tatttatcat gcatataatg gattatcttc
     1621 aaggcgttca catacacttt cgccaaccag cgcatcattt gctcatactt agccatcacc
     1681 tcatcgtatt ccagatactc gccgcgaacc ggctcgaaca tcggggctac ctgtacgccg
     1741 ctgcgctcat cccgtcctcc gttgatcgca tataataaac atttcgccaa attcgcacgg
     1801 gctccgaaga actgcatctg cttaccgatc ttcataggag aaacgcaaca agcgatacca
     1861 taatcatctc cataatccgg acgcatcaaa tcgtcgttct catattggat agccgacgta
     1921 tcgatagaga ccttggcaca gaaacgtttc cagttctcgg gagcgttctt aaaccacaga
     1981 accgtcaaat tgggttctgg tgccggacct agattatata atgtatggag ataacgatag
     2041 gaagttcttg ttaccaaaga acggccatcc actccttggc cgcccaagga ctctgttacc
     2101 caaaccggat caccggagaa caaatcattg tattccggcg tacgtaagaa acgaacgata
     2161 cgcaacttga tgatcagctg gtctaccaat tcttgcgcct cctcttcggt caacttaccc
     2221 tcacggatgt ctttctcgat ataaatatcc aaaaacgtag aagtacgtcc gatagacatc
     2281 gccgcgccat cttggtcttt taccgccgcc agataagcta agtacacaaa ctgaactgcc
     2341 tcacgagcgt tttgtgccgg acgagtcaca tcaaagccat agctagcgca cattttctcg
     2401 aacgctttca aagccttgat ctgttcggaa atctcctcac ggctctggat gatctcttcc
     2461 gtgatctctt ggatatccaa acgattcaag aaacgctttt tctcctcgat caagattgcc
     2521 gtaccataca gagcgacacg acgatagtca ccaataatac gtcctctacc atatgcgtcg
     2581 ggcagaccgg tgataatagc cgaacgacgt gcggcaatca tctcttccgt atatgcggag
     2641 aaaacgccct cgttatgcgt cttacggtac ttcgtgaaga tctctttcgt catcgggtcc
     2701 aaatgataac cataagcttc caagctattc tcaaccatac ggataccgcc cttcgggaag
     2761 ataccacgtt tcagcggagc atcggtttgt aacccaacga tcacctcatt ctccttatca
     2821 atatagcccg ggccatatac atcgatccct tgaggtaatt ttgtctcggc gtcataaact
     2881 cccttttccc tctcgacctt aaacatctcg gtcagcttgt tccagacctt acgggtcttc
     2941 tcggaagaag cgactaaaaa ggaatcatcc ccttcataag gcgtatagtt actctggata
     3001 aaatctctta cattgatctc tcttttccag gtctcaccct taaagtgatc ccagcactca
     3061 ttctcaaatt tcataaacaa tcttatctaa taccacattt ttaccgccgc aaaggtagga
     3121 gaattatcgt catatttctg tcacatatgc tacaccagtt ctaaaatccc tataaattgc
     3181 gataaaaaag cttctcatcc ttcactattt taaaaggaca ttaccaataa atggggagtc
     3241 agacctaagg gcgaagaggc aaccatgccc aaaaagtaga tccgacacca agttttgaag
     3301 taaagcccac ttctccccct gccgcttcta cgatagctct ggagatcgtc aagccaagtc
     3361 cggtaccctg cgagaac
//
