LOCUS       c00059_gnlPro..         4678 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|NBCCEGKM_59.
ACCESSION   c00059_gnlPro..
VERSION     c00059_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-07 20:20:57
            Original ID  :: gnl|Prokka|NBCCEGKM_59
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 4678
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     protocluster    1..4678
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[2:1344]"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      3..1344
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="2000"
                     /detection_rule="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /neighbourhood="10000"
                     /product="Pyruvate2acetate-formate"
                     /protocluster_number="1"
     cand_cluster    1..4678
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="((PFL_acetate1 or PFL_acetate2) and
                     Radical_SAM)"
                     /kind="single"
                     /product="Pyruvate2acetate-formate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..4678
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Pyruvate2acetate-formate"
                     /region_number="1"
                     /rules="((PFL_acetate1 or PFL_acetate2) and Radical_SAM)"
                     /tool="gutsmash"
     CDS             3..605
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: PFL_acetate2"
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Gly_radical"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg59_1"
                     /sec_met_domain="Gly_radical (E-value: 6.2e-48, bitscore:
                     150.3, seeds: 99, tool: rule-based-clusters)"
                     /sec_met_domain="PFL_acetate2 (E-value: 5.6e-21, bitscore:
                     63.0, seeds: 33, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="DFEREGEYVPFGNNDDRTDSIAVDITEKFMEYLRQHQTYRKATPT
                     QSILTITSNVVYGKKTGTTPDGRPGGTPFAPGANPMNGRDTKGAVAALASVAKLPFQHA
                     HDGISYTFAVSPATLGKERDIQINNLVSLLDGYFTPDGGQHLNVNVFDRDLLLDAMEHP
                     EKYPQLTIRVSGYAVNFVKLTREQQLDVISRTINSSL"
     CDS             622..1344
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Pyruvate2acetate-formate: Radical_SAM"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1015:4Fe-4S single cluster domain (Score: 75.2;
                     E-value: 2e-23)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg59_2"
                     /sec_met_domain="Radical_SAM (E-value: 1.4e-36, bitscore:
                     114.9, seeds: 518, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLGRIHSLESFGTVDGPGIRFVVFMQGCPLRCLYCHNPDTWEVKS
                     GTPYRMEPEALLAEVLRYKNFIAKGGVTVTGGEPLLQPEFLKEFFRLCRENGIHTALDT
                     SGYICSGKALEVLEQVDLVLLDIKTIDAGLHPRLTAVKLDNTLRFLDELEKRGIPVWIR
                     HVIVPGLTDDDEALSKLAEYISSYNVVQKAELLPYHTMGAYKYEAQGLDYKLKGVEPLS
                     AERLANAKAIFMKHGITV"
     CDS             complement(1347..1652)
                     /locus_tag="ctg59_3"
                     /transl_table=11
                     /translation="MRRASLYNTFSASLLLVCSAWLSSCVSQIPLTKEKPVNNSTYQVE
                     YLFEHEGCKVYRFMDMGHYIYFTNCEGDVTSIESDSVRTVNRISRKPIIIKEQTLD"
     CDS             1928..2494
                     /locus_tag="ctg59_4"
                     /transl_table=11
                     /translation="MEPIINSQVPEFKVQAFQNGSFKTVSSEDIKGKWAIFFFYPADFT
                     FVCPTELVDMADKYGKFQEMGVEVYSVSTDSHFVHKAWHDASETIRKIKYPMLADPTGA
                     LSRAFGVMIEEEGMAYRGTFLVNPEGKIKIAEIHDNGIGRNADELLRKVEAAQFVASHP
                     NEVCPAKWKKGEATLKPSIDLVGKI"
     CDS             2616..4181
                     /gene_functions="biosynthetic-additional
                     (rule-based-clusters) Pyr_redox_2"
                     /gene_kind="biosynthetic-additional"
                     /locus_tag="ctg59_5"
                     /sec_met_domain="Pyr_redox_2 (E-value: 3.9e-49, bitscore:
                     155.8, seeds: 65, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MLDSAMKDQLSGIFSGLVSQYVFDIQVAPDHESRQELIDLLGDVA
                     SCSDHLSCSVADGEGLQFTIVKDGMPTGIRFRAVPNGHEFTSLLLAVLNSDGKGKNIPD
                     ESICARVRALNGPIKLTTYVSLTCTNCPDVVQALNVMATLNSRITHETVDGAINQKEVE
                     AMKVQGVPSVFADGKLINVGRSDLGELLSKLEAQYGINEGGVDNSVEKPVKNYDVLIVG
                     GGPAGAASAIYSARKGLNVAVIAERIGGQVKETVGIENLISVPQTTGKQLANDLLTHIN
                     DYPVDILEHRRVDKVELDGSAKLLTTSTGERFSAPALIVATGASWRKLNVPGEADYIGK
                     GVAFCPHCDGPFYKGKHVAVVGGGNSGIEAAIDLAGICSKVTVLEFMDELKADQVLQEK
                     AKSLPNVEVFLHSQSLEVVGDGDKVTGLRVKDRKTDAERVIDLDGIFVQIGLAANSGVF
                     RDVVETNKPGEIVIDAHCRTNVPGIYAAGDVSTVPYKQIIIAMGEGAKAALSAFEDRMR
                     GSIG"
ORIGIN
        1 tcgacttcga acgcgaaggc gaatatgtac ctttcggaaa caatgacgac cgcacggaca
       61 gtattgctgt cgacattacc gagaagttca tggaatatct gcgccagcat cagacatacc
      121 gcaaggcaac tccgacacaa tctatcctga cgatcacttc aaacgtggtt tacggaaaga
      181 aaaccggaac gactcctgac ggccgtccgg gtggtacgcc gttcgctccg ggagccaacc
      241 cgatgaacgg acgcgacacg aaaggggctg ttgccgcttt ggcttctgtt gccaaactgc
      301 ctttccagca tgcgcatgac ggtatctcct atacatttgc cgtttcaccg gctacattgg
      361 gtaaggagcg cgatattcag ataaataatt tggtgagcct gttggatggc tattttacgc
      421 ctgacggtgg ccagcatctg aatgtgaacg tgttcgatag ggacttgttg ttagatgcaa
      481 tggagcatcc ggagaaatac ccgcagctta cgatccgcgt ttcgggctat gccgtcaact
      541 tcgtgaagct gacacgcgaa cagcagctcg acgtcatctc gcgcaccatc aacagcagtt
      601 tataattgtc cattgattaa tatgttgggg cgtatacatt ctttagagag tttcgggacc
      661 gttgacggtc ccggaattcg ctttgttgtt ttcatgcagg gatgtccgct tcgttgtctg
      721 tattgccaca atccggatac ttgggaggta aaaagcggga cgccttatcg aatggaaccg
      781 gaagccttgc ttgccgaagt gctgcgctat aagaatttta tcgctaaagg tggtgttacc
      841 gtgaccggtg gtgagccttt gttacaacct gaattcctta aagagttttt ccgcctttgc
      901 cgtgaaaacg gtattcatac ggcgttggac acttccggct atatctgttc cggtaaagca
      961 ttggaggtgc tcgaacaggt cgatctggtt ctgttagata tcaagacgat cgacgccggt
     1021 cttcatcccc gtttgacggc cgtaaaactc gataatactt tacgctttct tgatgaactg
     1081 gagaaacgcg gcatccctgt atggattcgc cacgtgatcg tgcccggcct gacggacgat
     1141 gatgaagcct tgagcaagct ggccgaatat atcagttcat ataacgtggt ccagaaagcc
     1201 gaacttctgc cttatcatac aatgggagct tacaaatatg aggcacaagg cctggactat
     1261 aagctgaaag gtgtggaacc gctttcagca gagcgtctgg ccaatgcgaa agcgatcttt
     1321 atgaaacacg ggataaccgt ataagcctaa tcgagtgtct gctctttgat gatgatcggt
     1381 ttacggctga tccggttgac tgtgcggaca ctgtcgcttt caatactggt aacatctccc
     1441 tcgcagttgg tgaaatagat gtaatgtccc atatccatga aacgatagac cttacaccct
     1501 tcgtgttcga acagatattc gacttgataa gtggaattgt ttaccggttt ttcctttgta
     1561 aggggtattt gtgagacaca tgaagaaagc catgcgctgc aaacgagcaa tagacttgct
     1621 gaaaatgtat tatacaaact agctcttctc atcttcgaat atttttaagt taacatccaa
     1681 agttaacaat taaagaatca catggtatga tcgaaaggta ttttacctac aattaggtat
     1741 atttttattg ttccgaaaaa cattactttg cttcctattg tcatagtaga taagtatgct
     1801 tgcagataga aatccgtcta tcggcagata aaaattatca atttgaaaaa ttctttcaat
     1861 cagtatctta cgatttacct ttgtagggta aacaaaaata gtagtaacaa attaaatagt
     1921 aaaaatcatg gaaccaatta tcaattcaca agtacccgaa ttcaaagtac aggcttttca
     1981 gaatggtagt ttcaagacag taagtagcga agatatcaag ggcaaatggg ccatcttttt
     2041 cttctatcct gccgacttta cgttcgtatg tccgaccgaa ttggtcgata tggctgacaa
     2101 gtacggcaaa tttcaggaaa tgggtgtcga agtatattcc gtaagtacag actcgcactt
     2161 tgtacacaaa gcatggcatg atgcttccga aacgatccgt aagatcaaat atccgatgct
     2221 ggcagacccg acaggggctt taagtcgtgc gttcggtgtg atgattgaag aagagggtat
     2281 ggcttatcgc ggtacgttcc tggtaaatcc cgagggaaag atcaagattg ccgaaatcca
     2341 cgataacggt atcggccgta atgcagacga attgcttcgt aaagttgaag ctgcccagtt
     2401 cgttgcttct cacccgaacg aagtttgtcc tgcaaagtgg aaaaaaggcg aagcaacctt
     2461 gaagccgagc atcgacttgg ttggtaagat ctaaaaagaa tgatgtgcca attgattaat
     2521 gtgccaatat gccaattatt tatgaggcaa tacatcgctt tagtcgattg gtacattctt
     2581 taattggcac gttaataaat tgacatatta tttttatgtt agactcagca atgaaagacc
     2641 agctttcggg tattttctcc ggactggtat cccaatatgt atttgatata caagtcgctc
     2701 ccgatcacga aagccgtcag gagctaatag acctgcttgg tgatgtggca tcatgctcgg
     2761 atcatttgtc atgcagcgtt gcggatggtg aaggacttca gtttacaatt gtaaaagacg
     2821 gtatgcctac cggtatccgg tttcgtgcag tacctaacgg acatgagttt acatccttgt
     2881 tgttagctgt cctgaatagt gatggaaaag gtaaaaatat accggacgaa agtatctgtg
     2941 ctcgtgtcag agccttgaat ggtccgatca agctgacgac ttatgtttct cttacttgca
     3001 ccaattgtcc ggatgtagtg caggcactca atgtcatggc tactctcaat agccggatca
     3061 cccacgaaac ggtggatggc gctatcaacc aaaaagaagt tgaggcaatg aaagtacagg
     3121 gagtcccttc tgtttttgcc gatggcaaac tgatcaatgt ggggcgtagt gatttgggag
     3181 aattgctttc caaactcgaa gcccaatacg gaattaatga agggggtgtt gataactctg
     3241 ttgaaaaacc tgttaagaac tatgatgtgt taatagtggg gggtggacct gccggtgcag
     3301 cttctgctat ctactcggca cgtaaaggct tgaatgtcgc agtgatagcc gaacgtatcg
     3361 gaggacaggt caaagaaacg gtcggtatcg agaacctgat ttccgttcct cagactactg
     3421 gaaagcagtt ggcgaacgac cttttgacac atatcaacga ctatccggtc gatatactgg
     3481 agcatcgccg tgtggataaa gtcgaactgg atggatcggc gaaacttctg accacttcta
     3541 ccggtgaacg tttttctgct cctgccctta tcgtggcgac aggtgcaagc tggcgcaagt
     3601 tgaatgttcc gggcgaagcg gactatatcg gaaaaggtgt cgctttttgc cctcactgcg
     3661 atggtccgtt ctataaaggt aagcacgtag cggttgtcgg cggtggaaat tcgggtatcg
     3721 aagcggctat agatttggca ggcatttgtt cgaaagtgac ggttctggaa tttatggacg
     3781 aactgaaagc cgaccaagtg ttgcaggaaa aagcaaagag cttgccgaat gtggaagttt
     3841 tcttgcattc ccaatctttg gaagtggttg gtgacggtga taaggtaaca ggtcttcgtg
     3901 taaaagaccg taagacggat gcggagcggg tgatcgacct ggacggaatc tttgtccaaa
     3961 tcggcttggc agccaatagt ggcgttttcc gcgacgttgt cgaaacgaac aaaccgggtg
     4021 agattgtaat cgatgcgcat tgtcggacga atgttcccgg catctatgcg gcgggcgacg
     4081 tctcgaccgt accctataaa cagataatca tagcaatggg agaaggggca aaagcggctc
     4141 tatctgcttt tgaagaccgc atgagaggtt caatcggtta atatttagta tttgctatga
     4201 acgacgaagg tgtgcaaatt gaactacacg aatgtgtgtt caaaattgca caccttttct
     4261 tttttacgca caaagccccg actttcacaa cagggcaacc gcatcaaaat tttccaaggt
     4321 tgtgatctcc atacgaagtt tctacaaatc ctctatcact ctatcactac gaaggttgta
     4381 gctattaata ttcaagcgtt agaggagtga tagaggaaaa catcctgtat cactgcctct
     4441 atcactcctc taacaggacg tttttatcat atgattggaa accaatatat tgccttcttt
     4501 tgcttttgaa aaagcatata aaataatcga ataaacaaat gtaaaaacga atgcaatagg
     4561 tgttaaattg acgaaatacg gttcttcatt ccacaaaagc cctctctttt tttagcagaa
     4621 acaagcagat aattccgtaa acacccccat gttgccatcc ttaacatccg ggtgtaga
//
