LOCUS       c00301_gnlPro..         6584 bp    DNA     linear   UNK 01-JAN-1980
DEFINITION  gnl|Prokka|KNHHKJLI_301.
ACCESSION   c00301_gnlPro..
VERSION     c00301_gnlPro..
KEYWORDS    .
SOURCE      
  ORGANISM  
            .
COMMENT     ##gutSMASH-Data-START##
            Version      :: 1.0.0-1555cd7(changed)
            Run date     :: 2024-10-05 13:49:03
            Original ID  :: gnl|Prokka|KNHHKJLI_301
            NOTE: This is a single cluster extracted from a larger record!
            Orig. start  :: 0
            Orig. end    :: 6584
            ##gutSMASH-Data-END##
FEATURES             Location/Qualifiers
     CDS             complement(1..312)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Fer2_3"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1052:Succinate dehydrogenase-2Fe-2S iron-sulfur
                     cluster binding domain (Score: 61.8; E-value: 1.8e-19)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg301_1"
                     /sec_met_domain="Fer2_3 (E-value: 1.5e-20, bitscore: 61.8,
                     seeds: 38, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MDKDIKVTLKVWRQKSPKDKGQFETYQIDKINQSVSFLEMLDILN
                     ERLVSEGKEPIVFDHDCREGICGMCSLYVNGHPHGPATGATTCQLYTRRFNDGETITIE
                     "
     protocluster    1..6584
                     /aStool="rule-based-clusters"
                     /contig_edge="True"
                     /core_location="[0:3042]"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
                     /tool="gutsmash"
     proto_core      1..3042
                     /aStool="rule-based-clusters"
                     /tool="gutsmash"
                     /cutoff="10000"
                     /detection_rule="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /neighbourhood="10000"
                     /product="Fumarate2succinate"
                     /protocluster_number="1"
     cand_cluster    1..6584
                     /candidate_cluster_number="1"
                     /contig_edge="True"
                     /detection_rules="(Succ_DH_flav_C and FAD_binding_2 and
                     (Fer2_3 or Fer4_7 or SucD_FumR))"
                     /kind="single"
                     /product="Fumarate2succinate"
                     /protoclusters="1"
                     /tool="gutsmash"
     region          1..6584
                     /candidate_cluster_numbers="1"
                     /contig_edge="True"
                     /product="Fumarate2succinate"
                     /region_number="1"
                     /rules="(Succ_DH_flav_C and FAD_binding_2 and (Fer2_3 or
                     Fer4_7 or SucD_FumR))"
                     /tool="gutsmash"
     CDS             complement(340..2283)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: Succ_DH_flav_C"
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: FAD_binding_2"
                     /gene_functions="biosynthetic-additional (smcogs)
                     SMCOG1027:FAD binding domain (Score: 195.7; E-value:
                     4.7e-60)"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg301_2"
                     /sec_met_domain="FAD_binding_2 (E-value: 3.8e-61, bitscore:
                     195.7, seeds: 62, tool: rule-based-clusters)"
                     /sec_met_domain="Succ_DH_flav_C (E-value: 3.8e-39,
                     bitscore: 122.1, seeds: 739, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MTQIDSKIPQGPLAEKWKNYKDHQKLVNPANKRRLDVIVVGTGLA
                     GASAAASLAEMGFNVLNFCIQDSPRRAHSIAAQGGINAAKNYQNDGDSVYRLFYDTIKG
                     GDYRAREANVYRLAEVSNSIIDQCVAQGVPFAREYGGLLDNRSFGGAQVSRTFYARGQT
                     GQQLLLGAYSALSRMIGLNKVKMYTRHEMLDVVKVDGRARGIIARNLITGQIERFSAHA
                     VVVATGGYVNTFFLSTNAMASNGSAAWQCYKKGAYFANPCMVQIHPTCVPVKGDYQSKL
                     TLMSESLRNDGRIWVPKKLEDAKALQAGTKKGKDIPEADRDYYLERRYPAFGNLVPRDV
                     ASRAAKERCDAGFGVNNTGLAVFLDFSDAINRLGKDVVKAKYGNLFDMYEEITNDDPYE
                     TPMMIYPALHYSMGGLWVDYELMTSIPGLFAIGEANFSDHGANRLGASALMQGLADGYF
                     VLPYTIQNYLSDQIQVPRFSTDLPEFAEAEKGIKDRIKKLMSIKGKESVDSIHKKLGHI
                     MWEHVGMARDAKGLQEAIQMLKELKKEFWSNVYIPGEADNLNVELDKALRLADFIEIGT
                     LMAHDALDRAESCGGHFRTEHQTEEGEALRHDDKFMYVSCWEYQGEDKDPVMLKEDLNY
                     QFVVPQTRNYKK"
     CDS             complement(2317..3042)
                     /gene_functions="biosynthetic (rule-based-clusters)
                     Fumarate2succinate: SucD_FumR"
                     /gene_kind="biosynthetic"
                     /locus_tag="ctg301_3"
                     /sec_met_domain="SucD_FumR (E-value: 8.7e-77, bitscore:
                     245.5, seeds: 10, tool: rule-based-clusters)"
                     /transl_table=11
                     /translation="MWLLNSSIGKKLIMSVSGLFLVLFLLFHLSMNVAAAFSGEAYNMI
                     CSLLGANWYAVAATLVLAAGVVIHFVYAVLLTLQNRKARGNDRYAINARPKGVEWASQN
                     MLVLGIIIILFMVLHFSQFWYKMMFAELLGNHMVTLCDGTTTVSPQDGAAFINYYFQGN
                     ILNTVLYLVWYVALWFHLTHGFWSAIQTIGWSNTIWMNRWICISKIVATVICVVFAIIT
                     IVFYLNGVGGDYVAVCAGH"
     CDS             3281..4678
                     /locus_tag="ctg301_4"
                     /transl_table=11
                     /translation="MLQRKMLSFLFMMIFIPSVRAQWKDSTRFINKEHEFETTYNKISE
                     NKVYQMLRVPVPLFAISAITYGQGDKFRTLRNTHTPNFHYRYDDFLQYTPLLAVYGMKL
                     GGVESRSSWPRMIVSNAFSATIMAATVNSMKYTIKRERPDGSKNNSFPSGHTATAFMAA
                     TIMHREYGLTRSPLYSIGGYTVATATAFSRQLNNRHWLSDVLAGAGIGILSTELGYFLA
                     DLIFKDKGLNLPDKLRESAPKGGRPSFLEFAVGYSIINGSIEVGKNIRLSSPGATNVSV
                     KGGYFFNPYFGIGGEITALASPMAFDMSLYTGTTPSTKYDITRIHADPTGVFTFTAGPY
                     FSLPVASKLLLGTKFQAGYSYLVKNEVEMDLQEKHPPYGTINNIAFMKGNGSSNYVLRT
                     GISATGIVSRNLGIRMFVDYDYTFLKAPYEILTSLRDSQPVYGESHLSKHKIHYFTIGA
                     TVTALFW"
     CDS             complement(4682..6505)
                     /locus_tag="ctg301_5"
                     /transl_table=11
                     /translation="MNKTILLVVFCFSLFVSGYSQGIRFDSYPSHPRLLVKKGEEQKIK
                     SDLALNPEMLRLHNYLLGQCDDLITTPVLAYKKEGKRLLAVSREALRRIFYLSYAYRMT
                     GEDKYRLRAEQEMIAVCSFTDWNPSHFLDTGEMTMAVAIGYDWLFDRLSPDARRLVQEA
                     ILNKGFAPSRDKRYNWFLRATNNWNQVCNAGLVYGALAVLEDHPEEAREIIERALSTVG
                     LTLKGYGPDGAYPEGYNYWGYGTTFQVLLNAALESALGTDGGLSSAEGFLASARFMQFM
                     TGTTGKCFNFSDSRESVFALPAMYWFADKLQDPTLLWNEKDMLSRAELSFSDEESRFLP
                     LIPIYGSQYKMNELVPPAMKLWAANGQTPVVLVRTGWENGEGFYLGIKGGTASTSHAHM
                     DAGSFVFDASGVRWVQDLGMQEYYSLEKENVDLWNMSQNGQRWNVFRYNNLAHNTLTVN
                     GKYHDVNGFVPVTNIYSEAHKLGASLNMTDLFGGELENAVREVALINEKYLSVIDRVKA
                     GSQPATVRWTIVTGAIPRVLDSHMVELTKDGKCMQLIVDSPSVTSVSVLDNVSKNSYDA
                     SNEGTCRIVFDVSLESGQESDIKVRLVPAAD"
ORIGIN
        1 ttcgattgta atagtttcgc catcattgaa gcgacgcgta taaagctggc aagtagttgc
       61 gccggttgca ggtccgtgcg gatgtccgtt tacatacaaa gaacacatac cgcagatacc
      121 ttcacgacag tcgtgatcga atacgatcgg ctctttacct tcactgacca aacgttcatt
      181 caaaatgtct aacatttcca ggaaagaaac actctgattg attttgtcta tttggtaagt
      241 ctcaaattgt cctttatctt tcggactttt ctgacgccaa actttaagtg ttacttttat
      301 atctttatcc atgattctaa tttttgtgat tgatgattat tacttcttgt agttacgtgt
      361 ttgaggaaca acgaactgat agttcaaatc ttccttcagc ataaccggat ctttgtcttc
      421 accctggtat tcccaacaag atacgtacat gaacttatcg tcatgacgca gggcttcgcc
      481 ttcctcggtc tggtgctctg tacggaagtg tcctccacaa gattcggcac ggtccaatgc
      541 atcgtgtgcc atcaatgtac caatctcaat aaagtcagcc aaacgcaatg ccttatccaa
      601 ctctacgttc aggttatcgg cttcacccgg aatatataca ttactccaga actctttctt
      661 cagctctttc aacatctgga tcgcttcctg caaacctttt gcatcacgtg ccatacctac
      721 atgttcccac atgatatgac ccagtttctt atggatagaa tctaccgact ctttaccctt
      781 gatactcatc aatttcttga tgcggtcttt gatccccttt tcagcttctg caaactcagg
      841 caggtcggtg ctgaaacgtg gaacctggat ctggtctgac agatagttct gaattgtata
      901 aggcaataca aagtagccat cggccaatcc ctgcatcaac gcagaagcac ccagacggtt
      961 agctccgtgg tcagagaagt tagcttcacc gatagcgaac agacccggaa tagaagtcat
     1021 caattcatag tcaacccaca gaccacccat cgagtagtgc aatgcaggat aaatcatcat
     1081 cggagtttcg tatggatcgt cgttggtaat ttcttcgtac atgtcgaaca agttaccata
     1141 tttagctttt acaacatctt tacccagacg gttgatggcg tcggagaagt cgaggaacac
     1201 ggccagacct gtgttgttca caccgaagcc ggcgtcgcaa cgttctttag cggcacgtga
     1261 agccacgtca cgcggaacca ggttaccgaa tgccggataa cggcgttcca gatagtagtc
     1321 gcggtctgct tcaggaatgt ctttcccttt cttagttccg gcctgtaatg cttttgcatc
     1381 ttccagtttc ttcggaaccc agatacgacc gtcgttacgc aatgattcag acatcaaagt
     1441 cagcttagac tggtagtcac ctttcaccgg tacacaagtc gggtggatct gcaccataca
     1501 agggttagcg aagtaagcac ctttcttgta gcactgccat gcagcagaac cgttggaagc
     1561 cattgcgtta gtagacagga agaaagtatt tacataacca cctgttgcca ccactactgc
     1621 gtgagcagaa aaacgttcga tctgacctgt gatcaggtta cgtgcgatga taccgcgggc
     1681 acggccatcc accttcacaa cgtccagcat ttcgtggcgg gtatacatct ttactttgtt
     1741 caaaccgatc atacggctca aagcagagta agcacccagc aacaactgct gtccggtctg
     1801 accgcgggca tagaacgtac gggatacctg agcaccaccg aatgaacggt tatccagcag
     1861 accaccgtat tcgcgagcga aaggaacacc ctgtgctacg cactggtcga taatagagtt
     1921 ggaaacttca gccagacgat aaacgtttgc ttcgcgggca cgatagtcac cacctttgat
     1981 tgtatcgtag aacagacggt aaacagagtc accatcattc tgataattct ttgcagcatt
     2041 gataccaccc tgtgcggcga ttgagtgtgc gcgacgggga gaatcctgga tacagaagtt
     2101 caatacgttg aatcccattt ctgccagaga ggcagcagca gaagcaccgg ccagaccagt
     2161 accaactaca atcacgtcca gacgacgttt gtttgcgggg ttcaccagtt tctgatgatc
     2221 tttataattt ttccacttct cagccaacgg gccctgaggg attttagaat ctatttgagt
     2281 cataatgatt tattttcaat attgttacac cttaaattaa tgtcctgcac aaacagctac
     2341 atagtcacca cctacaccat tcaggtagaa aacgatagta atgatagcaa acactacgca
     2401 aataacggtt gctacaatct tggaaatgca gatccaacgg ttcatccaga tcgtgttact
     2461 ccaaccgata gtctgaatag cgctccagaa cccgtgagtc aggtggaacc acaaagctac
     2521 gtaccatacc agatacagca ctgtattcag gatattaccc tggaaatagt agttgataaa
     2581 cgctgcaccg tcctgaggag atacggttgt tgttccgtcg caaagtgtta ccatgtgatt
     2641 gcctaggagt tcggcgaaca tcatcttata ccagaactgg ctgaagtgca acaccatgaa
     2701 caggataatg ataataccca gtaccagcat gttctgagat gcccattcca cacctttcgg
     2761 acgagcgtta attgcataac ggtcgttacc gcgagcctta cggttttgca gtgtaaggag
     2821 aacagcatac acaaagtgaa tcacaacgcc tgctgccagc accaatgttg ctgccacggc
     2881 ataccaattt gctcccaaca atgaacaaat catgttatag gcttctcccg agaaagcagc
     2941 cgccacattc attgacaagt ggaacaataa gaacaaaaca agaaaaaggc ctgatacact
     3001 cattatcagc ttcttcccga tagaagaatt aagtaaccac ataagataat aatttaagtt
     3061 atttaattgt ttttagaaat tcatatcaat atactcaatt atcaggcaaa ggtatgctaa
     3121 atacacgtat cacgcaaacg attaaagaaa tatttcgcca atcaaaccta tacatttcag
     3181 gagggacaaa cggtaaatcc tattcaataa ataaattaac gaaaatgtaa ccagtactaa
     3241 ccggcatttt tatatcttcg tggcataatc aggggatcgt atgttacaac gtaaaatgct
     3301 gagcttctta tttatgatga tttttatacc gtctgttcgt gctcagtgga aagatagtac
     3361 gagattcata aataaagaac atgaatttga aaccacttac aacaaaatat cagagaataa
     3421 ggtataccag atgctgcgtg tccctgttcc tcttttcgcc atttcagcca taacatatgg
     3481 gcagggcgat aaattccgga cattgcgcaa tacgcatacg cccaacttcc attacagata
     3541 cgatgatttt ctgcaatata ctccgttgct tgctgtttac gggatgaagc tcggcggtgt
     3601 ggaaagtcgt agttcatggc cccggatgat cgtatcgaac gctttttcgg caacgatcat
     3661 ggccgctacc gtcaacagca tgaaatatac gatcaaacgc gaacgccccg acggctcaaa
     3721 gaacaactca ttcccttcgg gacacacggc aaccgctttc atggctgcta ccatcatgca
     3781 ccgggaatat ggtctgaccc gcagcccgtt atatagtatc gggggatata cagtcgccac
     3841 agccaccgct ttcagccggc aactgaacaa ccgccactgg ctgagcgacg tactggccgg
     3901 ggccggtatc ggcatcttat ccacagagtt agggtatttt ctggcagacc tgatcttcaa
     3961 ggacaaaggc ttgaatctcc ctgacaagct cagggagtct gcccccaaag gagggcgccc
     4021 ttctttcctg gagttcgccg taggatacag tatcatcaac ggaagtatcg aggtaggtaa
     4081 aaatatacgc ctgagctcgc cgggagcaac caatgtcagc gtaaaaggcg gttacttctt
     4141 caatccttat ttcggtatcg gcggcgaaat aacagcgctg gcaagcccga tggcattcga
     4201 catgtcgctt tatacaggaa caaccccttc aaccaaatat gacatcaccc gcatccatgc
     4261 cgatccgacc ggtgttttta cttttacggc agggccttat ttctcgttac ctgtagccag
     4321 taaattgctt ttaggaacaa agttccaggc cggatattcc tatctggtga aaaatgaagt
     4381 ggaaatggac ttacaggaaa agcacccccc atacggaacc ataaataata ttgcttttat
     4441 gaaaggcaac ggtagcagca actatgtttt acgcacaggc atttcagcga ccgggatcgt
     4501 cagccgcaac ctgggtatcc gtatgttcgt agattacgat tatacgttcc tgaaagcacc
     4561 ttacgaaatc ttaaccagcc tcagagacag ccaaccagtc tacggcgaga gccatttaag
     4621 caagcacaaa atacactatt ttacaatagg ggcaaccgta acggcgctgt tctggtaatt
     4681 atcagtcagc agcaggaacc agccggactt tgatatcgct ctcctgcccg ctttccagac
     4741 ttacatcgaa aacgatacgg caggttccct cattggaagc atcatagcta ttcttcgata
     4801 cattatcaag gacggacaca gaagttacgg atggactatc aacgatcagt tgcatacatt
     4861 tgccgtcttt tgtcagttcc accatatgac tgtccaatac acggggaatg gccccggtta
     4921 caatcgtcca tctgacagtt gccggctgac tgccagcttt tacacgatca atcaccgaca
     4981 gatatttttc attgatcaga gcaacttcac ggacggcatt ttccaattct ccgccgaaca
     5041 agtccgtcat attcagagaa gctccgagtt tatgcgcttc cgaatagata tttgtcacag
     5101 gtacaaaacc gtttacatca tggtatttac cgtttacagt cagtgtatta tgcgccaggt
     5161 tattataacg gaacacattc cagcgctgcc cgttctgcga catattccat aaatctacat
     5221 tttccttttc cagcgaataa tattcctgca tccccaggtc ctgcacccag cgaacacccg
     5281 aagcatcgaa cacaaacgat ccggcatcca tatgggcatg gctggtcgaa gccgttcctc
     5341 cttttattcc cagatagaaa ccttctccat tctcccaacc cgtacggacc agcacgacag
     5401 gcgtctgtcc gttagccgcc cacaatttca tagcaggagg caccagttca ttcattttat
     5461 attgtgaacc ataaatcgga atcaacggaa ggaaacggct ctcctcatcc gaaaaagaca
     5521 actccgcccg actcaacata tccttttcat tccaaagcag tgtagggtct tgcagcttgt
     5581 cggcaaacca gtacatagcc ggcaaagcaa agacactctc acgggaatcg ctgaagttaa
     5641 agcatttacc ggtcgttccg gtcataaact gcataaaacg ggcagaagcc agaaaacctt
     5701 cagccgaaga gagaccgccg tcagtcccca aggcggattc caacgcggca ttcaaaagaa
     5761 cctggaaggt agtcccgtat ccccaataat tatatccttc gggataagcg ccatcgggac
     5821 catagccctt caaagtcaag ccgactgtag ataaagcacg ttcaatgatt tcacgggcct
     5881 cttccggatg atcttctaaa acggcaagtg caccgtaaac cagtccggca ttacagacct
     5941 ggttccagtt attggtcgct ctcagaaacc agttatatct tttatccctc gacggggcaa
     6001 aacctttatt cagaatcgcc tcctgcacca gcctccgggc atccggagat aaccgatcga
     6061 acaaccagtc ataaccgatt gcaaccgcca tagtcatttc gcccgtatcc agaaaatggg
     6121 acgggttcca atcggtaaac gaacacacgg caatcatttc ctgctctgcc cgcaaacgat
     6181 acttatcctc tcctgtcatc cggtaagcat atgaaagata gaagatacgc cgcaaggctt
     6241 cacgcgatac ggccagcaac cgtttccctt cttttttata tgccagcaca ggcgtcgtga
     6301 tcagatcgtc gcattgccca agtagatagt tatgcaaccg gagcatctcc ggattaagag
     6361 ccagatcgga tttaatcttt tgctcctctc ctttctttac cagcaaccgg ggatgagaag
     6421 ggtaagaatc aaagcgtatt ccctgtgaat accccgaaac gaacagggaa aagcaaaaca
     6481 caacaagcag gatagtttta ttcatacagg tattatattt aattggagtt acaaacttac
     6541 ataaaaattg agatttataa gcgcgtctaa taaaaataaa gacc
//
